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Offlinelindens
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Spore syringe on agar
    #26077628 - 06/27/19 03:55 PM (4 years, 6 months ago)

Using spore syringes of GT on agar, this is the result after 4 days.
I'm not sure how much you're supposed to squirt onto the agar but i wanted to get one of those black dots in each dish as seen in the first pic:

in the second pic i wasn't so lucky, only the clear liquid of the syringe made it in:

Are the black dots the actual GT spore? Do these look fine or is it too early to tell?


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Offlinelindens
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Re: Spore syringe on agar [Re: Trich]
    #26077668 - 06/27/19 04:18 PM (4 years, 6 months ago)

Thanks a lot! Will set up a few more plates with 1 drop of the syringe this time, and shake up that syringe to disperse the spores.

Another question, the agar i am using was made about 2 months ago (haven't had to chance to move forward until now), pressure cooked and kept sealed in a pyrex bottle (then gelled it up again in a microwave), is it possible it could've expired and is a source of the bacteria?


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Offlinelindens
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Re: Spore syringe on agar [Re: Yasuke]
    #26077772 - 06/27/19 05:13 PM (4 years, 6 months ago)

oooo i'll try this as well, i have some fresh loops. When you get it red hot, would a minute suffice for it to cool down before putting some solution on the loop?


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