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InvisibleEonTan
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Registered: 08/18/04
Posts: 468
Loc: very south
Re: I am looking for contaminated prints... [Re: LaughingJim]
    #3558426 - 12/29/04 06:01 PM (19 years, 1 month ago)

Grafting is grafting. Budding is just adding a single bud. Splicing is actually done at the Genetic level. That is why I asked.

All you are doing is Grafting a Scion, which can contain one or more buds, onto a rootstock. Upside Down. Most first time grafters Do this by accident quite frequently, that is why I found it ironic that they would award a patent to the TECHNIQUE, becasue it happens by accident frequently.

The Primary meristem (trunk) of a Tree is no different then the Axillary meristems of the same tree. A graft is a Graft. A single bud being grafted to a branch of a tree is BUDDING. Budding is Grafting.

Grafting is the broad term that refers to all forms of budding and grafting( whip, etc...)Regardless of whether it is a single bud on a branch, or the entire apical meristem of the rootstock is being replaced by a new variety. It is still GRAFTING.

Splice tends to refer to Adding genes from one plant to another. A splice as you are lableing it does not do this. You are simply grafting a apical meristem of one variety onto the rootstock of another. There is no genetic exchange or fusion. There is phenotype change based on the combination of scion/rootstock. Dwarfing, etc...

If buds are not removed from the rootstock, they will produce phenotypes consistent with the rootstock not the scion. Everything below the graft, is one variety, everything above is the other.

The term splicing is to general.

As far as your seeking resistant Strains of Cubensis by seeking ones that grow from contaminated spores, the resistance will not be passed on to the entire population of substrains that result from spores from that dikaryon. To find genetic resistance, you would have to isolate monokaryons of contaminate free spores, and test each of these in the presence of the known contaminat, and also matings of each of these in the presence of the known contaminat. Once you establish which monokaryons are responsible in which combinations you can then use these to possible breed resistance into new strains that may lack the resisatance. This is purely GENETIC RESISTANCE. Most resistance will probably rely on Environmnetal and Genetic variables. IE certain nutrients might be needed to create resistance in certain Substrains of certain strains.

Either way. The resistance will not breed true to all substrain originating from Spores of the DIKARYON that was resistant. Only some of the offspring will be resistant.

In the legal mushroom world you can buyand sell and trade CULTURES. Individual Dikaryons, so Your technique of random emmergence of resistant substrains, and this more eficient longer and more envolved technique both require the maintaining of DIKARYONS and the use of these clones. Not spores.

As far as resistance to competitors and pathogens, I would say the Puerto Rican is the Champ. But it is relative not each and every substrain is vigorous.

Good luck. If you find some resistant substrains, please clone and make duplicates, LOTS and LOTS of duplicates. You will need to test them. Remember, the contaminat itself Recombines it's DNA and the resistance is going to be for certain Variants of the Contaminant tested against, NOT all Substrains of the Contaminant. You see the Trich in your soil might be a tad bit differrent then the Trich in mine. Even in your soil there might be more then one Trich variant of a single species.

The most resistant Varieties of plants on the planet still succomb to disease, they just do so at more economically fisable levels.


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InvisibleEonTan
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Registered: 08/18/04
Posts: 468
Loc: very south
Re: I am looking for contaminated prints... [Re: EonTan]
    #3558479 - 12/29/04 06:17 PM (19 years, 1 month ago)

Now if you find a monokaryon from one strain PR and a monokaryon from another Strain GT and both are found to be the primary donors of resistance to their STRAINS. You might be in business. Mate the two, and possibly all of the resulting spores of that Dikaryon might contain the resistance when they mate amongst themselves?

Personally I prefer to just EXCLUDE COMPETITORS FROM MY GROW. If I were going to invest The TIME and MONEY it would be towards developing resistance in Commercial Edible MEdicinal Shrooms and the resistance would be towards PATHOGENS, not competitors.


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InvisibleCosm
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Registered: 05/07/04
Posts: 448
Loc: somewhere
Re: I am looking for contaminated prints... [Re: LaughingJim]
    #3558885 - 12/29/04 08:21 PM (19 years, 1 month ago)

i have contaminated reishi spores if you want some i have plenty.


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OfflineLaughingJim
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Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
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Re: I am looking for contaminated prints... [Re: EonTan]
    #3626974 - 01/14/05 02:09 AM (19 years, 17 days ago)

Quote:

EonTan said:
Splicing is actually done at the Genetic level.




I don't want to flame...

Genetic mutation can only be done by splicing, not the other way around... Splicing is not ONLY genetic, but it can be.

I never genetically spliced my audio tapes! I cut and tape, like we do with the plants.

(I thought I elaborated, that it was not the upside-down part that was patented, rather the variant and the method.)

Yes, if the parent is a survivor, then there is a high chance that the children/spores will be also. They get thier genetic code from the parent, so there is at least a 50% chance that the spores from the parent will have that same trait. No gene special tools and diagnostics are needed, just visual identification.

If I purposely drop these spores into a pile of already contaminated substrate, and they push it back or dominate, then I have succeded.

This "Random" emergence is one that already occurs, I have seen it, now I just have to increase the occurance of it.

A car is a car, if these repel the "Common" green mold that we all get, then it will repel it here, or there, on a plain, on a train, even on green eggs and ham.

There will always be acceptions, and for others there will not be. Molds don't adapt like viruses. They MUST have ideal situations to reproduce, and change very little. That is why they release Billions of spores, because they are a genetically almost incapeable of mutation, thanks to billions of years of simplicity. The green mold you see now, is almost the same as the green mold the dinosaurs had growing under thier nails.


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Offlinesean.staw5
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Registered: 05/07/19
Posts: 59
Last seen: 4 years, 2 months
Re: I am looking for contaminated prints... [Re: LaughingJim]
    #26065135 - 06/21/19 06:53 AM (4 years, 7 months ago)

why?


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