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Offlinestuckinatree90
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first time failed, won't give up. * 1
    #26043846 - 06/10/19 03:10 PM (1 year, 8 months ago)

hey guys, I started 2 rye grain bags with pe6 and put them in compost I got from mycohaus. none grew and all of my trays spoiled. I went by the book. I tried to keep my area as clean as possible. airing and fanning it. keeping sure its humid enough.
I got some more spores and sterilized some wbs. now I have 7 jars of TC. 2 of them seem to be complete, but I don't think it's wise to jump into this without a professional opinion.
do these jars seem OK to put into a bulk substrate? am i even growing mycelium? lol they've been growing for i'd say about 2 weeks now.







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OfflineProfessor X
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Re: first time failed, won't give up. [Re: stuckinatree90] * 1
    #26043855 - 06/10/19 03:14 PM (1 year, 8 months ago)

I can't tell if it's just bad pictures but those appear to be heavily contaminated. Did you use a MS syringe? You should start with agar.


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OfflineShaperDreaming
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Re: first time failed, won't give up. [Re: stuckinatree90]
    #26043863 - 06/10/19 03:17 PM (1 year, 8 months ago)

Your phone seems a bit like a potato in picture quality... so it's hard to say. The bottoms look not quite finished. The myc is super thick with these guys, which could indicate a few issues (looks a bit bacterial, and possible mold), but I think you're on the right path! When the bottom looks covered in white you might be in good shape, and try to take a better picture in the mean time :smile:


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OfflineLogicaL ChaosM
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Re: first time failed, won't give up. [Re: stuckinatree90]
    #26043924 - 06/10/19 03:46 PM (1 year, 8 months ago)

Those last few jars kinda look like they are infected but it could just be the lighting/camera.


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Offlinestuckinatree90
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Re: first time failed, won't give up. [Re: ShaperDreaming]
    #26043932 - 06/10/19 03:49 PM (1 year, 8 months ago)

sorry for the bad pictures





maybe those are a bit better. its kind of hard to get a good picture with this phone. i was really worried it would be mold. it looked very fluffy and something about that seemed off to me. i just figured that the spores were loving the seeds. it showed life within a few days and really picked up quickly.


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OfflineFractalMind
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Re: first time failed, won't give up. [Re: LogicaL Chaos]
    #26043934 - 06/10/19 03:50 PM (1 year, 8 months ago)

Millet based WBS seems way more resistant to contamination than rye. I have at least double the comtams using rye. Rye grain really should only be used for sclerotium.

Break them up one more time, if they are fully colonized after they recover you should be ok to move on to the next step.


Edited by FractalMind (06/10/19 03:52 PM)


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OfflineShaperDreaming
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Re: first time failed, won't give up. [Re: FractalMind]
    #26043948 - 06/10/19 03:57 PM (1 year, 8 months ago)

Here's the thing, they don't look perfect. They are bacterial at the very least. Would I try to spawn them? Yeah, coir's cheap and who know's what you'll get. It's not deadly looking. Just monitor the grow closely and if it turns nasty colors dump it out.

Another thing, if you want to see how it's doing just shake the shit out of the jar until all the grains are (mostly) loose and bouncing around. Give it 48 hours to see if it starts regrowing fluffy mycelium. If so, let it recolonize then you're good to spawn.


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Offlinestuckinatree90
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Re: first time failed, won't give up. [Re: ShaperDreaming]
    #26043990 - 06/10/19 04:16 PM (1 year, 8 months ago)

shaking the crap out of these jars wont hurt them at all? i mean... if it's bacteria then it doesnt really matter, huh? haha
thanks i'll try that. i have some sterilized mushroom compost. would it be beneficial in any way to put coco coir in it or just go straight up compost?


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OfflineJimAndGiantShrooms
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Re: first time failed, won't give up. [Re: stuckinatree90]
    #26044014 - 06/10/19 04:25 PM (1 year, 8 months ago)

No, it will not hurt the jars. Shaking half-way colonized jars is standard practice as it redistributes the mycelium and speeds colonization.


Edited by JimAndGiantShrooms (06/10/19 04:26 PM)


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OfflineShaperDreaming
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Re: first time failed, won't give up. [Re: JimAndGiantShrooms]
    #26044185 - 06/10/19 05:50 PM (1 year, 8 months ago)

Yeah, I often have to use the palm of my hand to smack jars against. It doesn't feel fun! Some people have broken jars over their hands this way (who are you strong fuckers?!), and Roger Rabbit recommends bouncing the jar off a bike tire... So whatever you choose just choose something that is strong and soft at the same time (i.e. it doesn't work when you beat it against a bed, too soft).


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Re: first time failed, won't give up. [Re: ShaperDreaming]
    #26044260 - 06/10/19 06:16 PM (1 year, 8 months ago)

Pasteurize compost at 160-165ish degrees Fahrenheit for 90 mins, sterilization of compost is not recommended. You can do this in a water bath of some sort or use a foil pan in the oven. Candy thermometers are readily available at grocery stores.

In theory, even if there's a small bacterial contamination, if your mushrooms eventually fully colonize the substrate, they will have become the dominant species in the jar and you will have a higher degree of success.

Breaking up jars approximately once weekly on a pillow, bike tire, or something soft enough not to break the jar, is standard procedure. What you have seems to be very well colonized and healthy, and so breaking it up may allow the rest of the grains to fully colonize.
If they do not recover completely  I would say that the bacteria is too established to get efficient growth and may not fully establish itself on the bulk substrate.

I have seen slightly contaminated casing still fruit, albeit less efficiently. Probably you will only get 1 flush.


Edited by FractalMind (06/10/19 08:06 PM)


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Offlinestuckinatree90
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Re: first time failed, won't give up. [Re: FractalMind]
    #26052288 - 06/14/19 02:16 PM (1 year, 8 months ago)

update. those 2-3 jars didnt survive. it was definitely bacteria. although all of my other jars recovered just fine.

thanks for the help, guys


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