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Offlinejphilly
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Registered: 11/09/03
Posts: 24
Last seen: 19 years, 6 months
how long is too long???
    #2604231 - 04/26/04 10:46 AM (19 years, 11 months ago)

I incubated 10 WBS about a month ago, i have been soo busy lately i havent had time to case. How long is too long for WBS to colonize. about half have no colinization and about 1/4 are about 98%, the other 1/4 about 1/2. What needs to be done to make this work??? please help.

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InvisibleMagash
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Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: how long is too long??? [Re: jphilly]
    #2604292 - 04/26/04 11:11 AM (19 years, 11 months ago)

Sounds like a air exchange. WBS should take no longer then two weeks.


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InvisibleMESCALATO
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Registered: 05/16/02
Posts: 1,025
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Re: how long is too long??? [Re: jphilly]
    #2604299 - 04/26/04 11:12 AM (19 years, 11 months ago)

how are they being icubated?
what kind of filter, tyvek,or polyfill?
strain might help too. give the 98% ones just a little more time, you could probably get rid of the ones that did not germinate, also how old were the spores used?


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Offlinejphilly
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Registered: 11/09/03
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Last seen: 19 years, 6 months
Re: how long is too long??? [Re: MESCALATO]
    #2604403 - 04/26/04 11:42 AM (19 years, 11 months ago)

the strain was tex, yb, and af.
i used polyfil, but no other filter.
jars in holder, then that in another one filled 1/3 with water.
covered w/ towel and heated at 82. there were some problems with temp in the beginning. first it was too hot for like 4 days, then i turned the heater off and it got too cold for like 4 days. That was the main problem.
The one that is like 98%...do you think it would be better to do a G2G or just case now.

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InvisibleMESCALATO
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Registered: 05/16/02
Posts: 1,025
Loc: Central Alabama
Re: how long is too long??? [Re: jphilly]
    #2605214 - 04/26/04 03:20 PM (19 years, 11 months ago)

do a little g2g and case the rest of that jar.....if that sounds good to you........
how hot did it get in the first 4 days?


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