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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26052537 - 06/14/19 02:51 PM (4 years, 8 months ago)

Asura's tek is sound.  And a good write up.


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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26052561 - 06/14/19 03:04 PM (4 years, 8 months ago)

Quote:

MacMerdin said:
Quote:

1WithU said:
Is that from Sara's old spores?




Yes.  And I'm getting a print from dOurd3n so I'll have 2 strains going.





Sweeeeeeet!  Curious if you see an differences side by side of the vars.  If I were to cut my "Warrens" print in half (thus no longer "sterile", but probably not sterile anyways) would you like half the print for a threeway side by side by side comparison?  Or is that just too much for you?


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26052594 - 06/14/19 03:26 PM (4 years, 8 months ago)

I have enough on my plate.  Thanks.

Suggestion though.  Have you thought of making swabs with the print instead of cutting it?  You could probably get 5-10 swabs per print.  And would be more sterile if you did it in a SAB.


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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26052659 - 06/14/19 04:02 PM (4 years, 8 months ago)

Quote:

MacMerdin said:
I have enough on my plate.  Thanks.

Suggestion though.  Have you thought of making swabs with the print instead of cutting it?  You could probably get 5-10 swabs per print.  And would be more sterile if you did it in a SAB.




That's a GREAT idea!  It'll be a whiiiiile before first fruits to print, and surely newbies will join in this thread soon wanting some.  A few swabs might find a good home in the near future.  BTW still waiting for Subfinder's prints to come in the mail.  Hoping for next week.  Hope to get a plate started ASAP.  Gonna hunt locally this weekend (been drizzling today).  Ok, gonna make a few swabs in SAB and once d0urd3n runs out in his giveaway I can take care of a few others.  Thanks for the great suggestion MacMerdin!


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26052684 - 06/14/19 04:19 PM (4 years, 8 months ago)

Actually since it is my idea, I will do the same with my d0urd3n print.  Now I have to learn how to swab.. lol


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OfflineAsuraS
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin] * 1
    #26052691 - 06/14/19 04:21 PM (4 years, 8 months ago)

Quote:

MacMerdin said:
Actually since it is my idea, I will do the same with my d0urd3n print.  Now I have to learn how to swab.. lol




You would not believe how convenient sterile q-tips are. I buy them in bulk and always seem to use them up!

And as a side note, I swear I've pointed about 5 different people to your pan tek this week. You might be getting
some :pm: :lol:


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Invisibled0urd3n
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: Asura]
    #26052772 - 06/14/19 05:16 PM (4 years, 8 months ago)

I'll try and remember to send you my fattest print then Mac if you're gunna make swabs out of it.


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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: d0urd3n]
    #26052801 - 06/14/19 05:53 PM (4 years, 8 months ago)

Quote:

d0urd3n said:
I'll try and remember to send you my fattest print then Mac if you're gunna make swabs out of it.




This collaboration is awesome!!

Thanks 1WithU for starting this experiment. And thanks dOur3n for the print!!!


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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26053016 - 06/14/19 08:25 PM (4 years, 8 months ago)

Quote:

MacMerdin said:
Quote:

d0urd3n said:
I'll try and remember to send you my fattest print then Mac if you're gunna make swabs out of it.




This collaboration is awesome!!

Thanks 1WithU for starting this experiment. And thanks dOur3n for the print!!!




Indeed it is!  What a great group of people!  And the fun has only just begun...

You're welcome!  I am grateful to have found a way to contribute something worthwhile to this great Shroomery community!  Truly a pleasure knowing that you & others are enjoying this, and I am having fun here too!


--------------------
Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: Asura]
    #26053036 - 06/14/19 08:42 PM (4 years, 8 months ago)

Quote:

Asura said:
Quote:

MacMerdin said:
Actually since it is my idea, I will do the same with my d0urd3n print.  Now I have to learn how to swab.. lol




You would not believe how convenient sterile q-tips are. I buy them in bulk and always seem to use them up!

And as a side note, I swear I've pointed about 5 different people to your pan tek this week. You might be getting
some :pm: :lol:




Is there a good thread to post a link to on this thread for q-tip teks, both how to make, and how to use?  I might have an extra print (kept some reserved) that might be best to disect onto q-tips to accommodate more people... but they would need to know what to do with a swab once they get it.

OMG how did I not notice MacMerdin's Pan Cake Tek post before?!?!?!?
https://www.shroomery.org/forums/showflat.php/Number/18357343&nbsp; Shall read it too to gather ideas that might translate to cincts too.  Thanks MacMerdin & Asura for sharing such valuable information!


--------------------
Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26053041 - 06/14/19 08:46 PM (4 years, 8 months ago)

Quote:

MacMerdin said:
Actually since it is my idea, I will do the same with my d0urd3n print.  Now I have to learn how to swab.. lol




:sunny:


--------------------
Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26053403 - 06/15/19 03:21 AM (4 years, 7 months ago)

Using a swab is easier than using a print since the spores are already on the device you use to inoculate (the swab).  Just put it to agar.

Making them is what I need to learn.


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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26053443 - 06/15/19 04:07 AM (4 years, 7 months ago)

Quote:

MacMerdin said:
Using a swab is easier than using a print since the spores are already on the device you use to inoculate (the swab).  Just put it to agar.

Making them is what I need to learn.




Is there something more to it then PCing a bunch of qtips in a container, swiping tip across print, seal in baggie, all in SAB?

I will look for a tek on how to use one too to post a link so that people know how to use it once they get it.  This could be some folks first time playing with agar or AS.  Will find a tek to make agar and nutrient cardboard too to share posts.


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



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OfflineZakkery
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26053471 - 06/15/19 04:38 AM (4 years, 7 months ago)

Dont mind me, just following along out of interest and the good vibes in this thread.


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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: Zakkery]
    #26053644 - 06/15/19 07:42 AM (4 years, 7 months ago)

1WithU

You're a hard worker.

From what I gather it is better to use medical swabs since the stick is much longer so you don't have to reach into the agar dish potentially contaminating it.

Pluse medical swabs already come sterile in the little bag they come in..

From what I gather is put a print in a SAB.  Open swab in SAB and swab print.

Hopefully you can find us a good tek to follow.


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Offline1WithU
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: MacMerdin]
    #26053668 - 06/15/19 08:10 AM (4 years, 7 months ago)

Thank you for the compliment MacMerdin!

I had a thought and am interested in opinions.  As a side project with DnDRnD who kindly offered to send me Jalisco spores from a syringe on paper (wet), I had what I hope would be a pragmatic solution for our Cinct dissemination project.

What if from my print from Subfinder I do this?

Cut coffee filter paper into half inch squares.  PCed.  Put a tiny smudge of black spores on each paper.  Put two papers in a little ziplock baggie.  Mail those out.  Then recipients could simple take with tweezers one of the sheets out of the bag (reserve the second sheet for another attempt later if necessary) and drop the paper face down on an agar plate or other agar substitute (I love the simplicity of 1teaspoon brf to 1Tablespoon water for starting spores, then transfer to clean).  I believe that this could be a simple way of taking my print and making enough to send out to say 20 more people.

I am curious to hear other's thoughts on this idea.

Edit:  I think this could be called "Seed Sheets"


--------------------
Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"



Edited by 1WithU (06/15/19 08:13 AM)


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OfflineFailboat
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26053677 - 06/15/19 08:18 AM (4 years, 7 months ago)

I like your seed sheets idea. I got some spores on a card, I assume I must use a loop or swab to use em. I tried with a scalpel handle and IDK yet lol


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OfflineMacMerdin
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26053684 - 06/15/19 08:23 AM (4 years, 7 months ago)

Interesting concept 1WithU.

Only way to know is to try I guess.

Also interesting you mentioned BRF/water agar substitute.  I was just reading about that for my lions mane.


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Edited by MacMerdin (06/15/19 08:24 AM)


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OfflineMr. Funguy
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: 1WithU]
    #26053743 - 06/15/19 09:15 AM (4 years, 7 months ago)

Quote:

1WithU said:
Cut coffee filter paper into half inch squares.  PCed.  Put a tiny smudge of black spores on each paper.  Put two papers in a little ziplock baggie.  Mail those out.  Then recipients could simple take with tweezers one of the sheets out of the bag (reserve the second sheet for another attempt later if necessary) and drop the paper face down on an agar plate or other agar substitute (I love the simplicity of 1teaspoon brf to 1Tablespoon water for starting spores, then transfer to clean).  I believe that this could be a simple way of taking my print and making enough to send out to say 20 more people.

I am curious to hear other's thoughts on this idea.

Edit:  I think this could be called "Seed Sheets"




Wrap them in foil like an acid tab, and you will be perfect...
Also for BRF Agar. The ratio is usually 2gramsbrf/500mlh20...


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OfflineKapnDank
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Re: Blue Pearls Explorer's Thread - Panaeolus Cinctulus Sclerotia - Blue Truffles [Re: Mr. Funguy]
    #26053853 - 06/15/19 10:41 AM (4 years, 7 months ago)

MacMerdin everything I remember reading states they take the swabs from the fruit and not the print. The print should still work but it may be a little more difficult vs swabbing some gills. That's just speculation though
Edit: also they may only swab fruits because the fruits they were talking about were hard to print. I've heard asura mention using swabs. Perhaps they can chime in.


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Edited by KapnDank (06/15/19 10:49 AM)


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