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mushboy
modboy
Registered: 04/24/05
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Making Liquid Culture 48
#26022486 - 05/30/19 08:27 AM (4 years, 10 months ago) |
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MAKING LIQUID CULTURE
LC should ONLY BE MADE from clean agar plates. this is a point of logic not a 'can it be done' statement. for the sake of making the 10 millionth 'why did i fail?' thread, just take my word and DONT MAKE LCs WITH SPORES.
WHAT IS LC? LC would be the growing culture inside a nutrient rich liquid. the liquid is technically called a broth. this is also the case with agar. so if you see the word BROTH, it simply means the liquid you have prepared. AGAR and LC are the same thing(broths). only agar..has agar WHY LC?because mushrooms HOW TO MAKE THE BROTHmeasure out desired amounts of water. i like doing ~250ml water per broth. you can make gallon LCs if you want i do smaller amounts because they colonize quicker and 250ml of LC can last a long time. also cuts back on how much media you expose while inoculating and so on.. INOCULATION TEKSJOSEX BIOPSY POKE - mandatory reading WEDGE METHOD(tek TBA) LETS MAKE THE BROTHweigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water. add the powder first THEN add COLD water. this prevents clumping. do NOT use hot water to make broths the hot water can cook the grain powder giving you lumps shake it up good..sometimes i stash the prepared broth in the fridge for awhile(after grain cycles run) but you dont have too. cover with foil, back off the cap and pc 15min @ 15psi. liquids sterilize easily. 30min+ can caramelize the sugars/wastes time. i remove from the PC the moment psi hits zero and tighten the cap other pointsunused broths have long shelf lifes. ive let them sit for weeks and they are ready for inoculation when you are. i use unmodified lids. i screw the lid on tight after inoculation. the culture is suspended in liquid and they dont need much GE anyway. NUTE TYPES:you can use many nute sources to make broths. as with agar, the type of nute will determine clarity LIGHT MALT EXTRACTBRF unfilteredGRAIN WATERive really only made three types. light malt, brf and grain water. LC DISCLAIMERsome people say lc is advanced or contamination prone ect.. IME this is not correct. however, LC can and will exploit any cracks in your cultivation armour. grains prepped too wet? cultures not entirely clean? SAB technique not down?? youll grow nothing but green real truthhoney/sugary LCs are so 1995
Edited by mushboy (05/30/19 08:59 AM)
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Chwyn
Bacteria Rancher
Registered: 08/21/16
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Re: Making Liquid Culture [Re: mushboy] 1
#26022522 - 05/30/19 08:48 AM (4 years, 10 months ago) |
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Camera93
We got dicks like Jesus
Registered: 08/15/18
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Re: Making Liquid Culture [Re: Chwyn] 1
#26022597 - 05/30/19 09:33 AM (4 years, 10 months ago) |
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-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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SFS96
AstroMan
Registered: 12/09/18
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Re: Making Liquid Culture [Re: Camera93] 1
#26022626 - 05/30/19 09:45 AM (4 years, 10 months ago) |
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Good write up that’s about as easy as it gets
-------------------- How I make and preserve tea Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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mushboy
modboy
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Re: Making Liquid Culture [Re: SFS96] 5
#26022636 - 05/30/19 09:50 AM (4 years, 10 months ago) |
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i like using media bottles for LC because its easy to pour into grain jars.
the mechanics of inoculating the broth and then using the lc to pour into grain jars is the same as pouring petris or doing g2g/a2g. easy peasy
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ShaperDreaming
Weirdo
Registered: 10/30/18
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Re: Making Liquid Culture [Re: mushboy] 4
#26022640 - 05/30/19 09:53 AM (4 years, 10 months ago) |
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Nice! This ... kinda makes a tek I was going to write up unnecessary! Good work
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mushboy
modboy
Registered: 04/24/05
Posts: 32,782
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ooops.
post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people
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micelio
Song of Silence
Registered: 04/22/14
Posts: 1,547
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Re: Making Liquid Culture [Re: mushboy] 1
#26022662 - 05/30/19 10:00 AM (4 years, 10 months ago) |
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Nice Teks. What's nice about LC is It doesn't need the gas exchange. There for just a non modified lid will do, as long as you have some air gap in the jar.
I would like to know your method on how you disperse your lc into your grain jars..? Also the amount of lc to each quart grain jar?
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mushboy
modboy
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Re: Making Liquid Culture [Re: micelio] 6
#26022670 - 05/30/19 10:03 AM (4 years, 10 months ago) |
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i just pour them and eye ball it.
250ml of lc is enough for at least 25 jars. 50 if you got skills. i usually get about 30ish but im lazy like that.
i dont shake after inoculation either. just let them chill. i need to know if i contaminate from the liquid or from technique. impossible to tell if you shake shit up right away.
this means you did it correctly
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ShaperDreaming
Weirdo
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Re: Making Liquid Culture [Re: mushboy] 2
#26022675 - 05/30/19 10:07 AM (4 years, 10 months ago) |
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Quote:
mushboy said: ooops.
post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people
Haha, no oops, solid work here form a TC. I'll likely still post it, but it's been lowered in priority now
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micelio
Song of Silence
Registered: 04/22/14
Posts: 1,547
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Thanks for the info. I been using a 60ml. syringe for dispersing. I have to break that habit and just learn how to pour with accuracy. I'm always worried that I'll get my grain to wet.. I normally use 10 ml. per grain jar..
Edited by micelio (05/30/19 10:39 AM)
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mushboy
modboy
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Re: Making Liquid Culture [Re: micelio] 1
#26022740 - 05/30/19 10:41 AM (4 years, 10 months ago) |
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the only times ive had the grains get too wet is if i prepped them kinda wet
OR, if i use the lc before its ready(colonized). like this one.
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Camera93
We got dicks like Jesus
Registered: 08/15/18
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Re: Making Liquid Culture [Re: micelio] 1
#26022749 - 05/30/19 10:44 AM (4 years, 10 months ago) |
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Quote:
micelio said: Thanks for the info. I been using a 60ml. syringe for dispersing. I have to break that habit and just learn how to pour with accuracy. I'm always worried that I'll get my grain to wet.. I normally use 10 ml. per grain jar..
I wanna be able to aspirate to syringes so I can cap and store edible cultures. Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method
Mushboy, you think pouring for that tek is fine, repeatable?
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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mushboy
modboy
Registered: 04/24/05
Posts: 32,782
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Re: Making Liquid Culture [Re: Camera93] 1
#26022753 - 05/30/19 10:46 AM (4 years, 10 months ago) |
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i dunno. never done it before.
but i made this little cake by dumping some lc ontop but it took awhile to colonize.
id imagine the syringe injected lc would be better suited but aspirating into syringes is a different tek and requires modded lids.
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ShaperDreaming
Weirdo
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Re: Making Liquid Culture [Re: Camera93] 1
#26022764 - 05/30/19 10:53 AM (4 years, 10 months ago) |
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Quote:
Camera93 said: Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method
I've been messing around with Mudda bottles and grains, and let me just tell you that is NOT the route to go with them.
I started getting into LC simply because I want to keep doing mudda bottles for gourmet work and grains fucking suck with them. I have ideas on how to make grains work... but why when I can just pour in some LC. I'm a week or so out from my next test (I have 400ml of LC growing out right now, about 60% done), and I'll be pouring from media bottles (same as this tek essentially). I think, if anything, the small amount of extra hydration will be helpful for mudda bottles (the zip-lock twist kind).
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Camera93
We got dicks like Jesus
Registered: 08/15/18
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Nice!! Okay cool, Ill find some lid teks, Quote:
ShaperDreaming said:
Quote:
Camera93 said: Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method
I've been messing around with Mudda bottles and grains, and let me just tell you that is NOT the route to go with them.
I started getting into LC simply because I want to keep doing mudda bottles for gourmet work and grains fucking suck with them. I have ideas on how to make grains work... but why when I can just pour in some LC. I'm a week or so out from my next test (I have 400ml of LC growing out right now, about 60% done), and I'll be pouring from media bottles (same as this tek essentially). I think, if anything, the small amount of extra hydration will be helpful for mudda bottles (the zip-lock twist kind).
Yeah grains in the bottle is tough, I just did the same but didn't supplement and PC because I was pretty certain I was gonna make a mess in the SAB trying to pour the grain. I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir that one has been the fastest by far. Sub is just lipa teked fuel pellets, redistributed to the pints
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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sandman420
Saint PP
Registered: 06/17/04
Posts: 5,384
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Re: Making Liquid Culture [Re: Camera93] 2
#26022800 - 05/30/19 11:25 AM (4 years, 10 months ago) |
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Wow 0.4% malt, that's pretty low. I might have to try that and cut back on my malt usage haha. Higher percents like 1-2% definitely give more sediments with malt, no problem though. nice tek bud.
Edited by sandman420 (05/30/19 11:30 AM)
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ShaperDreaming
Weirdo
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Re: Making Liquid Culture [Re: Camera93] 1
#26022817 - 05/30/19 11:37 AM (4 years, 10 months ago) |
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Ahhh, I realize I'm talking the same shit in two threads.
Quote:
Camera93 said: I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir that one has been the fastest by far.
Yeah, the dude from FreshCap Mushrooms shows using a wooden spoon handle to create a hole in the center before pc, then pouring into that hole:
This was my next idea... but still you can't shake/distribute it. I feel like the best option would be to do LC and the hole in the center, the give it a mean shake after you close the lid (then unscrew it a 1/4 turn for GE).
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Camera93
We got dicks like Jesus
Registered: 08/15/18
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last derailing comment, but what about colonized dowels?
I agree tho, LC seems like the way to go for sure
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Psicomb
shroom or die
Registered: 01/13/18
Posts: 4,751
Loc: the womb
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Re: Making Liquid Culture [Re: mushboy] 1
#26022869 - 05/30/19 12:16 PM (4 years, 10 months ago) |
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Thank you mushboy! Good shit mon
-------------------- When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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