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OfflineStampy
Elephant in the Woods

Registered: 08/18/19
Posts: 212
Loc: N 42nd Parallel
Last seen: 1 month, 18 days
Re: Making Liquid Culture [Re: meaculpaUIO]
    #26349295 - 11/27/19 06:33 PM (5 years, 1 month ago)

Ok second go around, first jars were bunk,

2 media bottle diluted grainwater LCs with Josex poke
4 baby food jar grainwater LCs with agar scrape




The media bottles are the most watered down, the baby food jars are
all different strengths, haven't noticed much difference.
The white balance was off during pictures they aren't that dark really!
Any comments or criticisms?

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OfflinemeaculpaUIO
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Registered: 08/26/19
Posts: 956
Last seen: 5 years, 1 month
Re: Making Liquid Culture [Re: Stampy]
    #26349348 - 11/27/19 06:55 PM (5 years, 1 month ago)

I dont know shit about LCs. Made two transfers and went to bed

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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
Last seen: 16 hours, 51 minutes
Trusted Cultivator
Re: Making Liquid Culture [Re: meaculpaUIO]
    #26349398 - 11/27/19 07:17 PM (5 years, 1 month ago)

Ok mushboy, I have done all kinds of experiments here and...I'm back to high
nutes. 5g LME per 500ml. Yeah, there's sediment and the photo ops aren't
as nice, but it just grows out quicker.

And when everything is settled, there is still a clarity of the broth that
lets me know it's all working out.

Don't hate me!


--------------------
Cultivating Panaeolus cyanescens


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Offlinespiritlands
 User Gallery


Registered: 06/21/10
Posts: 1,616
Loc: Flag
Last seen: 1 year, 1 month
Re: Making Liquid Culture [Re: Asura]
    #26349433 - 11/27/19 07:43 PM (5 years, 1 month ago)

Beer lc


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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OG Cultivator
Re: Making Liquid Culture [Re: Asura]
    #26349502 - 11/27/19 08:42 PM (5 years, 1 month ago)

Quote:

Asura said:
Ok mushboy, I have done all kinds of experiments here and...I'm back to high
nutes. 5g LME per 500ml. Yeah, there's sediment and the photo ops aren't
as nice, but it just grows out quicker.

And when everything is settled, there is still a clarity of the broth that
lets me know it's all working out.

Don't hate me!





youre right though:cheers:

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InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Making Liquid Culture [Re: mushboy]
    #26353874 - 11/30/19 01:22 PM (5 years, 1 month ago)

Anyone have tried agave nectar LC? I found this.

Quote:

Gretchenmeister said:
Fuck yes it's my new favorite.  The stuff I made last night looks "darker" than the first batch I made.

I did liquid SITR, malt, and Light agave, the agave performed as well as the malt..which is my favorite, and had ZERO sediment. My grains colonized nicely and the bulk trays I fruited performed as I expect from this monoculture.

The first batch I did by eyeballing, and I used very little..just enough to tinge the water slightly yellow. Last night I used varied percentages and a different brand of Light Agave. Nocced up today with Chitwan and Burma. We will see how it does this time. Hope it does good cuz I only did one malt of each.  But yes based on my last trial of agave it rocks.



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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
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Re: Making Liquid Culture [Re: Mr. Alien]
    #26353919 - 11/30/19 01:41 PM (5 years, 1 month ago)

Normally, when I find something that works well I just stick with it :shrug: LME works
so well I don't feel the need to experiment. The only exception I have found is
zapotecorum. I've tried twice to make an LC with it and it just doesn't respond.


--------------------
Cultivating Panaeolus cyanescens


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OfflineFailboat
Free man

Registered: 02/01/18
Posts: 8,736
Last seen: 1 month, 1 day
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Re: Making Liquid Culture [Re: Asura]
    #26354425 - 11/30/19 06:34 PM (5 years, 1 month ago)


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,487
Last seen: 2 hours, 34 minutes
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Re: Making Liquid Culture [Re: Failboat]
    #26356139 - 12/01/19 07:33 PM (5 years, 1 month ago)

You adjust pc times for the stir bar?
Feel like the answer is no but...?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Making Liquid Culture [Re: Camera93]
    #26356165 - 12/01/19 07:48 PM (5 years, 1 month ago)

no

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OfflineEdmunter
Mr
Male User Gallery


Registered: 05/01/13
Posts: 5,699
Last seen: 3 months, 7 days
Re: Making Liquid Culture [Re: verum subsequentis]
    #26358722 - 12/03/19 09:03 AM (5 years, 1 month ago)

How many jars should I go for wit 250cl?  I want speed and optimum growth.



ALSO

Can I just inoculate another jar with this while doing g2g in front of the hood?

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OfflineEdmunter
Mr
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Registered: 05/01/13
Posts: 5,699
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Re: Making Liquid Culture [Re: Edmunter]
    #26358725 - 12/03/19 09:05 AM (5 years, 1 month ago)

Quote:

Edmunter said:
How many jars should I go for wit 250cl?  I want speed and optimum growth.



ALSO

Can I just inoculate another jar with this while doing g2g in front of the hood?




Obs im gunna test first

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InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Making Liquid Culture [Re: Asura]
    #26358746 - 12/03/19 09:20 AM (5 years, 1 month ago)

Quote:

Asura said:
Ok mushboy, I have done all kinds of experiments here and...I'm back to high
nutes. 5g LME per 500ml. Yeah, there's sediment and the photo ops aren't
as nice, but it just grows out quicker.

And when everything is settled, there is still a clarity of the broth that
lets me know it's all working out.

Don't hate me!




Isn't that only 1% ? I just made 500G of agar with 10grams of lme

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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,487
Last seen: 2 hours, 34 minutes
Trusted Cultivator
Re: Making Liquid Culture [Re: SpunkyMonkey88]
    #26358755 - 12/03/19 09:27 AM (5 years, 1 month ago)

anyone got a pic of LC on agar test?
I promise I tried the SF within the last 5 years, images ect.
Keyword was "LC test on agar"

I tried in this thread and get results of discussion but no pics.
I streaked mine last night and am looking for a rough time frame for growth


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
Trusted Cultivator
Re: Making Liquid Culture [Re: Camera93]
    #26358860 - 12/03/19 10:37 AM (5 years, 1 month ago)

"LC test on agar"




Those pics were probably at two days. I can't remember. I know that i can usually be fairly certain of cleanliness/ filthiness  at 2 or 3 days. Apparently i don't have any pictures of test plates a little farther along. This is surprising. Oh well.

@Edmunter, In my experience, a quick but not stingy free pour splurge drops about 15 cc, give or take 5 in either direction. I don't really trip on it though. I'd just make up as many bags as you want to fruit and stretch it across them.

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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,487
Last seen: 2 hours, 34 minutes
Trusted Cultivator
Re: Making Liquid Culture [Re: verum subsequentis]
    #26358875 - 12/03/19 10:51 AM (5 years, 1 month ago)

:heytherebadboy:
Awesome, Thank you Verum


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


Edited by Camera93 (12/03/19 10:53 AM)

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InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Making Liquid Culture [Re: Camera93]
    #26359220 - 12/03/19 02:23 PM (5 years, 1 month ago)

I need numbers!

What ratio LME to water?

I've heard anything from 2g to 5g per 500ml...

I thought agar was supposed to be 10%? Is it different with lcs?

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OfflineEdmunter
Mr
Male User Gallery


Registered: 05/01/13
Posts: 5,699
Last seen: 3 months, 7 days
Re: Making Liquid Culture [Re: SpunkyMonkey88]
    #26359237 - 12/03/19 02:29 PM (5 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
I need numbers!

What ratio LME to water?

I've heard anything from 2g to 5g per 500ml...

I thought agar was supposed to be 10%? Is it different with lcs?




2% not 10...... Usually 10 grams

So if you want a 2% nutes LC add 10g of lme to 500ml

I went lower and it worked for me.

Does anyone lC2LC

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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Re: Making Liquid Culture [Re: Edmunter] * 1
    #26359261 - 12/03/19 02:37 PM (5 years, 1 month ago)

No each LC gets it's own wedge. Going lc2lc just sounds like a bad idea..

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OfflineEdmunter
Mr
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Registered: 05/01/13
Posts: 5,699
Last seen: 3 months, 7 days
Re: Making Liquid Culture [Re: mushboy]
    #26360213 - 12/03/19 11:54 PM (5 years, 1 month ago)

Quote:

mushboy said:
No each LC gets it's own wedge. Going lc2lc just sounds like a bad idea..




So if you had a tested lc and you want to go to grains, why not add a little to another jar of nute?  I mean you need to test it anyway and mine would be in front of a hood.  It might shit the bed but it seems like a good idea, less vectors than dropping a wedge.....reason to try....mushrooms

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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Injection Grain Bag


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