Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Kratom Powder For Sale   Original Sensible Seeds High THC Strains

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
Offlinethe_sonic16
Mushy McMusherson
I'm a teapot User Gallery


Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 2 years, 17 days
Re: Making Liquid Culture [Re: mushboy]
    #26134234 - 08/13/19 05:38 PM (4 years, 5 months ago)

What he said. Also, there's no problem with contams as the broth cools and air is pulled back inside?


--------------------
Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: the_sonic16]
    #26134254 - 08/13/19 05:58 PM (4 years, 5 months ago)

Quote:

verum subsequentis said:
i put filters on my lids. Do you leave the lid loose during colonization to allow the culture to breath?




No I screw them tight after inoculation.

Quote:

the_sonic16 said:
What he said. Also, there's no problem with contams as the broth cools and air is pulled back inside?




Does it happen when pcing agar? Not unless you fuck up.


Extras: Filter Print Post Top
Offlinethe_sonic16
Mushy McMusherson
I'm a teapot User Gallery


Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 2 years, 17 days
Re: Making Liquid Culture [Re: mushboy]
    #26134267 - 08/13/19 06:11 PM (4 years, 5 months ago)

Word. Thanks, Mushboy.


--------------------
Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


Extras: Filter Print Post Top
OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 11 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: Tweeq]
    #26134413 - 08/13/19 07:48 PM (4 years, 5 months ago)

Quote:

Tweeq said:
Nice write up! If I may propose another simple recepy. For those of us who regularly eat boiled potatoes: mix some of the potato water that is left after boiling with some regular tapwater to get very clear LC






I'm going to try this. That looks pretty sweet, Tweeq.


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Making Liquid Culture [Re: primelines]
    #26134607 - 08/13/19 10:29 PM (4 years, 5 months ago)

Quote:

primelines said:
Quote:

ShaperDreaming said:
Looks like an agar puck dropped in there Sunny :thumbup:




Why do you only drop in a wedge? Not a huge chunk of an entire plate that's been cut up tic tac toe style?

Easier to break up/agitate or just less chance of contam being in a smaller wedge?




I highly recommend the Josex Poke, these ones were tiny wedges of agar though.

Like others say, less chance of contamination.  Don't need a big chunk, why bother?


Extras: Filter Print Post Top
OfflineTweeq
Tweeq of Nature
Male

Registered: 06/07/18
Posts: 2,043
Loc: Netherlands Flag
Last seen: 2 hours, 17 seconds
Trusted Cultivator
Re: Making Liquid Culture [Re: SunnyDayze]
    #26134700 - 08/14/19 12:06 AM (4 years, 5 months ago)

Thanks Mushboy and Asura! I'm not going back to grainwater. This is so much more 'see through' and colonizes just as fast. Btw, I have also never used stirring devices for LC


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Making Liquid Culture [Re: Tweeq]
    #26135264 - 08/14/19 10:22 AM (4 years, 5 months ago)

i don't give a rats arse about clear LC. It is pretty though


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: verum subsequentis]
    #26135293 - 08/14/19 10:37 AM (4 years, 5 months ago)

uhh.. not clear:nerd: LCs work just as well.



but the clear look is more visual i guess. it just looks so clean and pure


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Making Liquid Culture [Re: verum subsequentis]
    #26135396 - 08/14/19 11:33 AM (4 years, 5 months ago)

Quote:

verum subsequentis said:
i don't give a rats arse about clear LC. It is pretty though




Grain water lc is fun to watch.  I kinda like the murky stuff too.


Extras: Filter Print Post Top
Offlineprimelines
Agarth Brooks
 User Gallery

Registered: 02/25/16
Posts: 484
Last seen: 4 days, 11 hours
Re: Making Liquid Culture [Re: SunnyDayze]
    #26136282 - 08/14/19 07:38 PM (4 years, 5 months ago)

If there is still some undissolved LME floating around in the water after PC is that because it wasnt shaken enough before pc? Hope it's good.


--------------------


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Making Liquid Culture [Re: primelines]
    #26136305 - 08/14/19 07:51 PM (4 years, 5 months ago)

It's about dissolving the nutes into the liquid with heat. If i want it really chunk free I'll also strain it through cheese cloth before PC but it isn't needed. The myc will eat it all. Chunks are fine.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: verum subsequentis]
    #26136489 - 08/14/19 10:52 PM (4 years, 5 months ago)

Quote:

verum subsequentis said:
It's about dissolving the nutes into the liquid with heat.




thats not how you get clear clump free broth.

its also purposely written in the tek why you DONT use heat to 'dissolve' the nutes during the prep.


Quote:

add the powder first THEN add COLD water. this prevents clumping.

do NOT use hot water to make broths:nono: the hot water can cook the grain powder giving you lumps:facepalm:





nutes will be dissolved during the cycle but if you use hot water to prep then the powder wont dissolve.

Quote:

verum subsequentis said:
i don't give a rats arse about clear LC.




then dont give advice on how to make it:shrug:


Extras: Filter Print Post Top
OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 11 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: primelines] * 1
    #26136507 - 08/14/19 11:25 PM (4 years, 5 months ago)

Quote:

primelines said:
If there is still some undissolved LME floating around in the water after PC is that because it wasnt shaken enough before pc? Hope it's good.




You have to use a really low amount of LME or there will be sediment no matter what you do. I have 2 LC's going that are 2.5g LME + 20g dextrose and those
are pretty clear. This might be my new recipe. But the sediment doesn't matter. Myc seems to like it. Your jars just won't be as clear.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: Asura]
    #26136515 - 08/14/19 11:38 PM (4 years, 5 months ago)

whats the dextrose for?


Extras: Filter Print Post Top
OfflineEl Chupacabra
Stoned Ape
Male


Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 15 days
Re: Making Liquid Culture [Re: Asura]
    #26136516 - 08/14/19 11:39 PM (4 years, 5 months ago)

I just made my first LC. I made mine practically the same as I've been making my agar media... With beer.

Looks pretty clear too. Hope this cambo LC does well enough to save me some time expanding.:cool:
Thanks for this thread mushboy you rock :headbanger:


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: El Chupacabra]
    #26136518 - 08/14/19 11:43 PM (4 years, 5 months ago)

beer lc and no pic?:yuno:


Extras: Filter Print Post Top
OfflineEl Chupacabra
Stoned Ape
Male


Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 15 days
Re: Making Liquid Culture [Re: mushboy]
    #26136524 - 08/14/19 11:56 PM (4 years, 5 months ago)

Haha, I know better.


I literally JUST inoculated it so no growth :frown:
Beer has done me well in making agar culture media (or just "agar" as a normal person calls it) so I figure *fingers crossed* it should do well.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: El Chupacabra]
    #26136529 - 08/15/19 12:01 AM (4 years, 5 months ago)

:popcorn:


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Making Liquid Culture [Re: mushboy]
    #26136720 - 08/15/19 07:02 AM (4 years, 5 months ago)

There is a difference between clear and clump free, in my opinion. I've made a ton of lc and that's how it works for me. I don't give a rats about clear LC but i don't like chunks and sediment. Heating the water on the stove after adding it to the powder is how i attain semi chunk/ sediment free LC or agar. To make it nice and sediment free I'll strain through cheese clothe.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,263
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26136787 - 08/15/19 08:26 AM (4 years, 5 months ago)

Quote:

verum subsequentis said:
Heating the water on the stove after adding it to the powder is how i attain semi chunk/ sediment free LC or agar. To make it nice and sediment free I'll strain through cheese clothe.





Or follow my tek and use cold water.



Heating on the stove and cheese clothes? Hard pass.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Kratom Powder For Sale   Original Sensible Seeds High THC Strains


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 14,289 10 02/28/06 08:35 PM
by Limelight_Liver
* fidgeting with liquid culture Anonymous 1,317 6 03/02/03 09:17 AM
by Anonymous
* Starting a simple liquid culture method Garret_Hill 9,113 3 03/11/02 09:49 AM
by YourHighness
* Easy Liquid Culture Container Tek SixTango 20,216 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 17,741 7 05/05/20 03:40 AM
by poisoned
* liquid culture failure gray1 5,939 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,032 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,447 9 05/26/13 04:57 PM
by cronicr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
76,468 topic views. 31 members, 235 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.007 seconds on 13 queries.