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OfflineTweeq
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Re: Making Liquid Culture [Re: verum subsequentis] * 2
    #26133831 - 08/13/19 01:38 PM (4 years, 5 months ago)

Nice write up! If I may propose another simple recepy. For those of us who regularly eat boiled potatoes: mix some of the potato water that is left after boiling with some regular tapwater to get very clear LC



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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: Tweeq]
    #26133835 - 08/13/19 01:44 PM (4 years, 5 months ago)

:creepylurker:

..that is clear. Thanks man!


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InvisibleSunnyDayze
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Re: Making Liquid Culture [Re: mushboy]
    #26133846 - 08/13/19 01:51 PM (4 years, 5 months ago)

hmm...i'm boiling potatoes as we speak.... :awesomenod:


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: SunnyDayze]
    #26133862 - 08/13/19 02:05 PM (4 years, 5 months ago)

So, most of these now aren't using stir plates... any reason why? I've noticed that with a stir plate I can get close to a full 200ml of usable LC within 3-5 days with a stir plate.

Also, I've been going dirty on my recipe. 200ml water + 2.5g LME + 1tsp (approx) dextrose. I get much faster results and the culture looks clear within about 3-5 days, but still brown and full of neutes.

Quote:

verum subsequentis said:
Flame sterilize a loop and dip it in the culture. [...] Just be sure that the only thing that goes in or over your open liquid has been flamed.




How in gods name would you flame sterilize the whole loop enough to make it "clean" to go over the top? Seriously, not trying to be annoying, I'd rather do this than waste a whole syringe on testing it out.




Edited by ShaperDreaming (08/13/19 02:09 PM)


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OfflineLtLurker
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Re: Making Liquid Culture [Re: ShaperDreaming] * 1
    #26133867 - 08/13/19 02:09 PM (4 years, 5 months ago)

tilt your jar. Then you don't need to be above it with your hand and handle.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: LtLurker]
    #26133874 - 08/13/19 02:14 PM (4 years, 5 months ago)

Your answer is so simple...

:manofapproval:


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: LtLurker]
    #26133876 - 08/13/19 02:14 PM (4 years, 5 months ago)

Also use long ass loops.



I dont use stir bars because I dont need lc under a deadline.


Edited by mushboy (08/13/19 02:33 PM)


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InvisibleBph
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Re: Making Liquid Culture [Re: mushboy]
    #26133888 - 08/13/19 02:21 PM (4 years, 5 months ago)

Quote:

LtLurker said:
tilt your jar. Then you don't need to be above it with your hand and handle.





Ahhhhh. Tilt the jar.

Quote:

mushboy said:
Also use long ass lopes



I dont use stir bars because I dont need lc under a deadline.




That is a long ass loop. Now I can see how it all works. Gonna go rig me up something out of bailing wire. Nothing like getting your questions answered. Thanks everyone.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: Bph]
    #26133934 - 08/13/19 02:57 PM (4 years, 5 months ago)

Yep, the ol jar tilt. worth it's weight in gold


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Offlineprimelines
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26133995 - 08/13/19 03:37 PM (4 years, 5 months ago)

Sorry for all the questions I just want to make sure this will work:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26134006 - 08/13/19 03:41 PM (4 years, 5 months ago)

Just checking in on my liquid and I found something perfect for the conversation. The first two pics are the same lc. It's been neglected for a while, full of chunks and has an entire myc layer on top. I'm pretty sure it's clean. I'll throw it in a bag and check.... In the name of science. The other pictures are random other LCS that are tested on agar and clean. One is already in a bunch of bags and the other is on Deck.



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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26134007 - 08/13/19 03:41 PM (4 years, 5 months ago)

👍 that'll work.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26134028 - 08/13/19 03:52 PM (4 years, 5 months ago)

Honestly Verum, I'd feel pretty confidant with that mycelium island not being contam. I've let a couple go that ended up doing that, put them back on the stir plate and they went back under and turned out fine. My guess is that the myc prefers to not drown while it grows... this is very unscientific of me.

Though... when that happened it was a huge PITA to break up the myc and it got lodged in my syringe and... well, it eventually worked so :shrug: Still, not contaminated.

I'm excited to see that grow out.


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InvisibleSunnyDayze
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Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26134049 - 08/13/19 04:03 PM (4 years, 5 months ago)

These were forming on top too but I swirled a few days ago.  That layer is just suspended now. Very neglected but grew out clean :reckon:



As I was straining I had a thought....Shit, how about heavily salted potato water??  Can't boil potatoes without a shit ton of salt.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: SunnyDayze]
    #26134062 - 08/13/19 04:09 PM (4 years, 5 months ago)

Looks like an agar puck dropped in there Sunny :thumbup:

Also, good point on the salt. Have I ever commented that every time I make grains I have the strongest desire to add salt and pepper? Every. Damn. Time. I once had to stop myself, I had the damn box of salt in my hand!


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Offlineprimelines
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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26134085 - 08/13/19 04:20 PM (4 years, 5 months ago)

Quote:

ShaperDreaming said:
Looks like an agar puck dropped in there Sunny :thumbup:




Why do you only drop in a wedge? Not a huge chunk of an entire plate that's been cut up tic tac toe style?

Easier to break up/agitate or just less chance of contam being in a smaller wedge?


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: primelines] * 1
    #26134106 - 08/13/19 04:30 PM (4 years, 5 months ago)

most folks advise putting a tiny piece of agar into an lc to drop the chances of transferring something other than cube myc. Nutritious Liquid is a god damn feeding frenzy for any living thing. Any contam at all will completely ruin the entire lc. The same agar may do perfectly fine in a grain jar. A lot of peoples agar is not as clean as they think it is.

Also, LC colonizes plenty quickly with a tiny piece.


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Offlinethe_sonic16
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26134195 - 08/13/19 05:16 PM (4 years, 5 months ago)

So, for the vessel lid, any solid/regular vessel lid will work as long as it has a synthetic filter in it? (On account of the exploding and infection factors). Am I correct here? This is a rad tek for the LC itself, but the vessel construction/use still escapes me. Maybe I'm over thinking it.


--------------------
Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: the_sonic16] * 1
    #26134223 - 08/13/19 05:30 PM (4 years, 5 months ago)

I dont put filters on my lids. Dont want to mod the media bottles cap.

Leave the lid loose and covered with foil. Just like making agar. Same exact process.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: mushboy]
    #26134228 - 08/13/19 05:34 PM (4 years, 5 months ago)

i put filters on my lids. Do you leave the lid loose during colonization to allow the culture to breath?


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