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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 2 hours, 57 minutes
Trusted Cultivator
Re: Making Liquid Culture [Re: newtomyc]
    #26046530 - 06/11/19 06:28 PM (4 years, 9 months ago)

Quote:

mushboy said:
real truth
honey/sugary LCs are so 1995
:begone:





Uhh you can pry the dextrose from my cold, dead hands!

jk :lol:

Nice write up, man.

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InvisibleBph
Stranger
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Registered: 10/11/18
Posts: 1,466
Re: Making Liquid Culture [Re: Asura]
    #26046602 - 06/11/19 06:55 PM (4 years, 9 months ago)

Following this when I get home!!! Thanks mushboy!

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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Making Liquid Culture [Re: Bph] * 2
    #26046656 - 06/11/19 07:17 PM (4 years, 9 months ago)

Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
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Re: Making Liquid Culture [Re: sandman420]
    #26046741 - 06/11/19 08:00 PM (4 years, 9 months ago)

I agree with that

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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 2 months
Re: Making Liquid Culture [Re: sandman420] * 1
    #26047588 - 06/12/19 08:28 AM (4 years, 9 months ago)

Quote:

sandman420 said:
Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.




I recently had a 75% colonized LC of 400ml, it hadn't really done anything in a while so I decided to unscrew the media bottle 1/4 turn in case I was suffocating it (I have a tendency to keep my unmodded lids a little on the tight side unfortunately due to paranoia). 3 days later I noticed mold around the inner rim :facepalm: It'd been growing out for almost 4 weeks w/o issue and one little 1/4 twist and BAM green.

:allsodisappointed:

Yeah, prickly whore is an understatement.

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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
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Re: Making Liquid Culture [Re: ShaperDreaming] * 1
    #26047650 - 06/12/19 09:15 AM (4 years, 9 months ago)

I wouldn't recommend unprotected lids for LC. If you want to use plastic lids for LC, wether modded or un, I would recommend wrapping twice or thrice with cling a para while still in the flow or sab.

Also, an interesting observation is that that same contam would have made it in no matter what was in the jar. That's why I quit using unmodified plastic lids.

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Invisiblemicelio
Song of Silence
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Registered: 04/22/14
Posts: 1,547
Re: Making Liquid Culture [Re: verum subsequentis]
    #26047711 - 06/12/19 09:41 AM (4 years, 9 months ago)

I use six magnetic stirrers. I built all six using computer fans to drive the magnets. I don't use any speed controllers. I can probably build one for twenty dollars..
Last night I found at Amazon a stirrer for 29.80 dollars with one magnetic stir bar and free shipping.
Now that's a decent price for someone to get started in stirring your LC.

https://www.amazon.com/Magnetic-stirrer-magnetic-Stirring-Capacity/dp/B072K24X5P?ref_=Oct_BSellerC_318023011_0&pf_rd_p=9e5f3db3-eeed-5343-9148-b378931e1e87&pf_rd_s=merchandised-search-6&pf_rd_t=101&pf_rd_i=318023011&pf_rd_m=ATVPDKIKX0DER&pf_rd_r=ABPZKAQH09YN9XDAV0T3&pf_rd_r=ABPZKAQH09YN9XDAV0T3&pf_rd_p=9e5f3db3-eeed-5343-9148-b378931e1e87&ie=UTF8&linkCode=ll1&tag=theshro-20

:cookiemonster:


--------------------
     

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OfflineShaperDreaming
Weirdo
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Registered: 10/30/18
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Re: Making Liquid Culture [Re: micelio]
    #26047785 - 06/12/19 10:20 AM (4 years, 9 months ago)

Quote:

verum subsequentis said:
I wouldn't recommend unprotected lids for LC. If you want to use plastic lids for LC, wether modded or un, I would recommend wrapping twice or thrice with cling a para while still in the flow or sab.



That's a solid idea... shame I didn't think of it lol. I'm still very new at making LC, so these mistakes were totally expected. I have such little investment in this it's not funny. I have a jar of PE LC, my first that didn't contam. I've been letting it sit for almost a month now because I don't trust that it won't contam... I should probably pour it lol.

Quote:

micelio said:
Last night I found at Amazon a stirrer for 29.80 dollars with one magnetic stir bar and free shipping.
Now that's a decent price for someone to get started in stirring your LC.



That's been sitting in my cart for like a month now lol. I just need the $$$

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InvisibleElseta2
Away for a few months
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Registered: 05/14/19
Posts: 75
Loc: Faraway
Re: Making Liquid Culture [Re: ShaperDreaming]
    #26047808 - 06/12/19 10:32 AM (4 years, 9 months ago)

LC. What's the longest it can last in one sigle contaiter?


--------------------

Need prints of Zapotecorum Pm me

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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 7 hours, 36 minutes
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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26047813 - 06/12/19 10:33 AM (4 years, 9 months ago)

I used to have a couple digital magnetic stirrer hot plate combos. Never knew they ran like $500 till I had to give em up hah

Edited by Failboat (06/12/19 10:34 AM)

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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 9 months
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Re: Making Liquid Culture [Re: Failboat] * 1
    #26048362 - 06/12/19 02:59 PM (4 years, 9 months ago)

@shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)

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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
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Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26048717 - 06/12/19 05:44 PM (4 years, 9 months ago)

LC can  reliably last on the shelf for about a month or two.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineLtLurker
Lost Sailor
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Registered: 01/03/18
Posts: 7,535
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Re: Making Liquid Culture [Re: sandman420]
    #26048787 - 06/12/19 06:14 PM (4 years, 9 months ago)

:skol:

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Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: Making Liquid Culture [Re: LtLurker]
    #26048953 - 06/12/19 07:59 PM (4 years, 9 months ago)

:cool:

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OfflineMcGrimm
Professor of Peace


Registered: 04/24/17
Posts: 772
Last seen: 30 days, 19 hours
Re: Making Liquid Culture [Re: gizmo1]
    #26048958 - 06/12/19 08:03 PM (4 years, 9 months ago)

:crazy2::takingnotes:


--------------------
In Peace or Pieces we shall move forward.

Grimm

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InvisibleElseta2
Away for a few months
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Registered: 05/14/19
Posts: 75
Loc: Faraway
Re: Making Liquid Culture [Re: sandman420]
    #26050306 - 06/13/19 01:55 PM (4 years, 9 months ago)

Quote:

sandman420 said:
LC can  reliably last on the shelf for about a month or two.



In the fridge?


--------------------

Need prints of Zapotecorum Pm me

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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Making Liquid Culture [Re: Elseta2] * 1
    #26050328 - 06/13/19 02:07 PM (4 years, 9 months ago)

3 or 4 months is the max I have kept anything in the fridge personally.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineDactylium
Don't touch me I'm sterile

Registered: 08/12/16
Posts: 696
Last seen: 1 year, 17 days
Re: Making Liquid Culture [Re: verum subsequentis] * 2
    #26050415 - 06/13/19 02:58 PM (4 years, 9 months ago)

Quote:

verum subsequentis said:
@shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)




I wrap the threads of my jars several times with plumber's tape to form a nice seal with my plastic lids.

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OfflineShaperDreaming
Weirdo
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Registered: 10/30/18
Posts: 3,429
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Re: Making Liquid Culture [Re: Dactylium] * 1
    #26050439 - 06/13/19 03:13 PM (4 years, 9 months ago)

Quote:

Dactylium said:
Quote:

verum subsequentis said:
@shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)




I wrap the threads of my jars several times with plumber's tape to form a nice seal with my plastic lids.



:breakthrough:

That stuff is good to PC? Damn, genius, I've always loved that shit.

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OfflineDactylium
Don't touch me I'm sterile

Registered: 08/12/16
Posts: 696
Last seen: 1 year, 17 days
Re: Making Liquid Culture [Re: ShaperDreaming]
    #26050571 - 06/13/19 04:29 PM (4 years, 9 months ago)

Sure is, it's made of PTFE which has a melting point of 327C (620F in freedom units)

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds


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