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Invisiblemicelio
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Re: Making Liquid Culture [Re: Camera93] * 1
    #26022899 - 05/30/19 12:39 PM (4 years, 7 months ago)

Mushboy..
Do you use leftover lc to expand out to new batches or do you start out with a wedge from a new plate..

I use Josex one poke method allot. To be honest It always turns out to be three pokes or more for me..


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Edited by micelio (05/30/19 12:52 PM)


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Offlinesan pedro guy
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Re: Making Liquid Culture [Re: micelio] * 1
    #26023003 - 05/30/19 01:44 PM (4 years, 7 months ago)

Honey will not work? Slow to colonize?

I liked the 90’s ....


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Invisiblemicelio
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Re: Making Liquid Culture [Re: san pedro guy] * 1
    #26023062 - 05/30/19 02:12 PM (4 years, 7 months ago)

Your right.. forget the honey.  I find It to be very slow also....


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: micelio] * 1
    #26023572 - 05/30/19 06:05 PM (4 years, 7 months ago)

Quote:

micelio said:
Mushboy..
Do you use leftover lc to expand out to new batches or do you start out with a wedge from a new plate..





no. always start with fresh wedges.

im bias towards honey lc because i tried it a few times and i always failed. plus every noob tries it and its as cliche as prom night acne.


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Invisibleshroomify

Registered: 04/14/19
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Re: Making Liquid Culture [Re: mushboy] * 1
    #26046093 - 06/11/19 02:50 PM (4 years, 7 months ago)

Great stuff. Looking forward to the wedge inoculation :takingnotes:


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: shroomify] * 1
    #26046107 - 06/11/19 02:57 PM (4 years, 7 months ago)

:awesomenod:In about a week


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OfflineMLPismyOPSEC
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Re: Making Liquid Culture [Re: mushboy] * 1
    #26046158 - 06/11/19 03:24 PM (4 years, 7 months ago)

I think i know the answer here but it's worth the question. Josex recommends "one LC, one use, one session." I have a smaller SAB and can load 8-10 quart jars fairly comfortably but no more. I load them through the arm holes, so the top stays on the whole time. Would i be able to noc up the first 8-10 jars, loosely place the lid back on the LC, unload/reload, and noc 8-10 more?


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Invisiblesandman420
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Re: Making Liquid Culture [Re: MLPismyOPSEC] * 2
    #26046182 - 06/11/19 03:39 PM (4 years, 7 months ago)

Yea josex probably just meant use the whole jar of LC at once, don't take out half and use it and expect to use the other half a week later. I think that is good advice.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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OfflineFailboat
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Re: Making Liquid Culture [Re: sandman420] * 1
    #26046188 - 06/11/19 03:41 PM (4 years, 7 months ago)

What do you do about breaking up the mycelium for inoculation?


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Invisiblemicelio
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Re: Making Liquid Culture [Re: MLPismyOPSEC] * 1
    #26046199 - 06/11/19 03:47 PM (4 years, 7 months ago)

As long as you kept the lid contaminate free there's no problem..


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OfflineFailboat
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Re: Making Liquid Culture [Re: micelio] * 1
    #26046201 - 06/11/19 03:49 PM (4 years, 7 months ago)

So just shake it? I've always failed with LC, might try again soon.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Failboat] * 1
    #26046214 - 06/11/19 03:59 PM (4 years, 7 months ago)

Quote:

Quirkmeister92 said:
So just shake it? I've always failed with LC, might try again soon.



My success rate is much lower than I'd like, but I can make LC happen. Try with these lower nutes and fewer ingredients, it's a little easier to work with. I almost feel ready to start using tiny wedges to inoculate over a poke!


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Invisiblemicelio
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Re: Making Liquid Culture [Re: Failboat] * 1
    #26046215 - 06/11/19 04:00 PM (4 years, 7 months ago)

You don't want to insert anything into the lc jar.
What I would do is put the jar on a table/counter and without lifting the jar off the table make some circular motions to stir the lc. By not lifting the lc jar it keeps the lc level low where it won't touch the lid or jar threads..
Some people use glass, I use a Magnet stirrer. Some use marbles. But all inserted before pc cycle.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: micelio] * 3
    #26046244 - 06/11/19 04:14 PM (4 years, 7 months ago)

I just swirl it like it's a wine glass. I'm half tempted to get magnetic stirrers and a stirring plate for no other reason than I think it would look bad ass and super science-y!

:superscience:


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Invisiblemicelio
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Re: Making Liquid Culture [Re: ShaperDreaming] * 2
    #26046296 - 06/11/19 04:35 PM (4 years, 7 months ago)

I'm half tempted to get a glass of wine..Ha..


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: micelio] * 1
    #26046302 - 06/11/19 04:37 PM (4 years, 7 months ago)

Quote:

micelio said:
I'm half tempted to get a glass of wine..Ha..



:hereyougo:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: ShaperDreaming] * 2
    #26046344 - 06/11/19 05:01 PM (4 years, 7 months ago)

I'd highly recommend getting stir bars and making our buying a stirrer. It's not needed but makes the LC grow faster, the myc status in tiny tiny little pieces with no clumps and it's truly set and forget.

You can absolutely use half, reload the SAB, and use the other half. You can even use half, let it sit for a couple weeks and use the other half. Just remember with LC, one wrong move and everything after that goes to shit. I highly recommend getting an LC on agar three or four days before use. Even if it's already been tested and used prior. If it's been sitting long, I test it again.


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26046360 - 06/11/19 05:10 PM (4 years, 7 months ago)

Quote:

ShaperDreaming said:
I just swirl it like it's a wine glass.




That's all I do. A good shake sometimes too :wink:


Quote:

verum subsequentis said:
You can absolutely use half, reload the SAB, and use the other half. You can even use half, let it sit for a couple weeks and use the other half.




:whathesaid:

Just like agar bottles.


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Offlinenewtomyc
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Re: Making Liquid Culture [Re: ShaperDreaming] * 1
    #26046434 - 06/11/19 05:43 PM (4 years, 7 months ago)

Quote:

ShaperDreaming said:
I just swirl it like it's a wine glass. I'm half tempted to get magnetic stirrers and a stirring plate for no other reason than I think it would look bad ass and super science-y!

:superscience:




That's funny!! When I initially started buying stuff, I bought a stirrer. Never have used it, but it looks cool on my work bench!


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: newtomyc] * 2
    #26046506 - 06/11/19 06:19 PM (4 years, 7 months ago)

Use it! You'll be surprised


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