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sandman420
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Re: Making Liquid Culture [Re: Camera93] 2
#26022800 - 05/30/19 11:25 AM (4 years, 7 months ago) |
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Wow 0.4% malt, that's pretty low. I might have to try that and cut back on my malt usage haha. Higher percents like 1-2% definitely give more sediments with malt, no problem though. nice tek bud.
Edited by sandman420 (05/30/19 11:30 AM)
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sandman420
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Yea josex probably just meant use the whole jar of LC at once, don't take out half and use it and expect to use the other half a week later. I think that is good advice.
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sandman420
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Re: Making Liquid Culture [Re: Bph] 2
#26046656 - 06/11/19 07:17 PM (4 years, 7 months ago) |
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Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.
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sandman420
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LC can reliably last on the shelf for about a month or two.
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sandman420
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Re: Making Liquid Culture [Re: Elseta2] 1
#26050328 - 06/13/19 02:07 PM (4 years, 7 months ago) |
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3 or 4 months is the max I have kept anything in the fridge personally.
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sandman420
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LOL you are totally wasting your time wrapping your threads with plumbers tape. The mason jarrs do not seal on the threads. They seal by pressing the top of the jar against the bottom of the lid where there is typically a gasket. There is absolutely no way you can get a seal on those course threads they are like 5 threads per inch and 1/16 inch tall. If you want to use plastic lids for LC just buy silicone gaskets they work perfectly. Not tattler brand the clear thick silicone gaskets on ebay or amazon. Easy peasy.
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sandman420
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Re: Making Liquid Culture [Re: Dactylium]
#26050844 - 06/13/19 06:47 PM (4 years, 7 months ago) |
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No. The lid is just sealing it like it usually does. The plastic lids only leak occasionally.
And even if it does work, that's totally a hillbilly approach because mason jars are just plain not designed to seal that way and there is an easily available way that IS designed to seal it properly.
Edited by sandman420 (06/13/19 08:14 PM)
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sandman420
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Re: Making Liquid Culture [Re: mushboy]
#26051115 - 06/13/19 09:25 PM (4 years, 7 months ago) |
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Yea your tek with the media bottle is really well done and will work great if people follow it 
Definitely superior to jars. Smaller hole = less chance of contam falling in the open lid when inoculating etc.
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sandman420
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Same thing...they are designed to seal by pressing the top of the jar on the bottom of the lid. The threads only exist to apply pressure on this area, not to make a seal. It is GL-45 threads of you want to google it if you don't trust me. Here is some pictures one of one of my GL-45 caps, this is a 3 piece GL-45 cap you can see it has a o ring that sits on the underside of the metal part that the plastic top presses on to seal it. Not all Gl-45 caps have the o-ring, just this particular type. But they are still designed to make their seal on the underside of the lid like literally every small capped liquid container in existence. Water bottles jugs etc...
Edited by sandman420 (06/14/19 08:40 AM)
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sandman420
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edited that post with a pic
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sandman420
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Yea that's the common type. These are custom pneumatic lids that must hold pressure.
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sandman420
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Re: Making Liquid Culture [Re: Nichrome] 1
#26463222 - 02/01/20 08:33 AM (3 years, 11 months ago) |
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You really only need to whip an LC with a stir bar once or twice a day for less than a minute so I don't know why you would complain about the noise it's not that loud. There's not much difference running it 24/7 and many stir plates will overheat your lc if you do that anyway because the motors can get warm especially when dialed down low because of the rheostat. If you do want to run your LC stirring for 24/7 you better test your stir plate by letting it run for a few days and feel if the base plate is warm where the LC sits. I once ruined a whole bunch of LC after finding out my old 4 position stirrer gets warm when run 24/7.
Edited by sandman420 (02/01/20 08:40 AM)
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sandman420
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Re: Making Liquid Culture [Re: Asura] 1
#26463344 - 02/01/20 10:00 AM (3 years, 11 months ago) |
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Once I did PE with 24/7 stirring and a star shaped bar and it grew these little spiked balls of mycelium that looked like naval mines. It was wild and I regret not getting any pics.
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sandman420
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Re: Making Liquid Culture [Re: Puduwoke]
#26463446 - 02/01/20 11:06 AM (3 years, 11 months ago) |
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oh snit wow yep just like that.
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sandman420
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Re: Making Liquid Culture - LME + How is 1g / 600 ml H2O 4% Sugar? [Re: MushBrain1]
#27380138 - 07/09/21 06:29 AM (2 years, 6 months ago) |
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Quote:
MushBrain1 said: Hey Stranger,
How is 1g / 600 ml H2O 4% Sugar for the common version of LME Liquid Media Culture? I calculate 0.16% by weight. If you can please explain the very light % with LME.
Thanks
4% LME makes for a very dark broth. Most people don't use that. I'm running 0.5%. It doesn't really matter but anything over about 1% starts to get pretty dark. The mycelium grows fine in low nutrient broths, at least with LME.
Edited by sandman420 (07/09/21 06:30 AM)
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