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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: Chwyn] * 1
    #26022597 - 05/30/19 09:33 AM (4 years, 7 months ago)

:camping:


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineCamera93
We got dicks like Jesus
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Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: micelio] * 1
    #26022749 - 05/30/19 10:44 AM (4 years, 7 months ago)

Quote:

micelio said:
Thanks for the info. I been using a 60ml. syringe for dispersing. I have to break that habit and just learn how to pour with accuracy. I'm always worried that I'll get my grain to wet..
I normally use 10 ml. per grain jar..




I wanna be able to aspirate to syringes so I can cap and store edible cultures. Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method

Mushboy, you think pouring for that tek is fine, repeatable?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: ShaperDreaming] * 1
    #26022781 - 05/30/19 11:04 AM (4 years, 7 months ago)

Nice!!
Okay cool, Ill find some lid teks,
Quote:

ShaperDreaming said:
Quote:

Camera93 said:
Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method



I've been messing around with Mudda bottles and grains, and let me just tell you that is NOT the route to go with them.

I started getting into LC simply because I want to keep doing mudda bottles for gourmet work and grains fucking suck with them. I have ideas on how to make grains work... but why when I can just pour in some LC. I'm a week or so out from my next test (I have 400ml of LC growing out right now, about 60% done), and I'll be pouring from media bottles (same as this tek essentially). I think, if anything, the small amount of extra hydration will be helpful for mudda bottles (the zip-lock twist kind).





Yeah grains in the bottle is tough, I just did the same but didn't supplement and PC because I was pretty certain I was gonna make a mess in the SAB trying to pour the grain.
I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir
that one has been the fastest by far.
Sub is just lipa teked fuel pellets, redistributed to the pints


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: ShaperDreaming] * 1
    #26022829 - 05/30/19 11:44 AM (4 years, 7 months ago)

last derailing comment, but what about colonized dowels?

I agree tho, LC seems like the way to go for sure


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: Failboat]
    #26356139 - 12/01/19 07:33 PM (4 years, 1 month ago)

You adjust pc times for the stir bar?
Feel like the answer is no but...?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: SpunkyMonkey88]
    #26358755 - 12/03/19 09:27 AM (4 years, 1 month ago)

anyone got a pic of LC on agar test?
I promise I tried the SF within the last 5 years, images ect.
Keyword was "LC test on agar"

I tried in this thread and get results of discussion but no pics.
I streaked mine last night and am looking for a rough time frame for growth


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26358875 - 12/03/19 10:51 AM (4 years, 1 month ago)

:heytherebadboy:
Awesome, Thank you Verum


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Edited by Camera93 (12/03/19 10:53 AM)


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
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Re: Making Liquid Culture [Re: Digit]
    #26425275 - 01/09/20 07:13 PM (4 years, 19 days ago)

Quote:

verum subsequentis said:
"LC test on agar"




Those pics were probably at two days. I can't remember. I know that i can usually be fairly certain of cleanliness/ filthiness  at 2 or 3 days. Apparently i don't have any pictures of test plates a little farther along. This is surprising. Oh well.





--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: A.k.a]
    #26425299 - 01/09/20 07:27 PM (4 years, 19 days ago)

That is how I did it.
I seen good recovery in about 2 or 3 days.


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: A.k.a]
    #26426221 - 01/10/20 09:23 AM (4 years, 19 days ago)

day 2


day 5


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: sandman420]
    #26463494 - 02/01/20 11:31 AM (3 years, 11 months ago)



My rustywhyte is doing the same
I keep it on a low swirl and crank it once a day or so to really blend
Think im gonna shut off for the weekend and let it fluff up


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: mushboy]
    #26507177 - 02/27/20 08:20 PM (3 years, 10 months ago)

Rustywhyte and ATL#7


Quote:

Bobbit said:

My question to the mushboy, how much more growth can I expect?

Your mix seems low nute when compared with older LC teks. Can I expect the whole jar to fill? Or is there an upper limit?

I guess you give a good shake before pouring into grain jars?




Is there much more growth to expect? These are 150ml


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Edited by Camera93 (02/27/20 08:23 PM)


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: fightingcherries]
    #26682613 - 05/20/20 06:00 AM (3 years, 8 months ago)



made some broth up, and PC'd for 20 min at 15psi

there was a lot of sediment, but I went ahead and inoculated it via agar wedge last night.

any reason to be thrown off by the sediment, or will it be okay (if clean) just not as pretty?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: rickyswamps]
    #26682684 - 05/20/20 06:47 AM (3 years, 8 months ago)

thanks ricky :thumbup:


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 23 hours
Trusted Cultivator
Re: Making Liquid Culture [Re: Pastywhyte]
    #26773803 - 06/22/20 08:45 AM (3 years, 7 months ago)



7 LC broths PC'd last night.

150ml at 1% with around 1.5g LME

Stir bar


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Unfilter Print Post Top
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