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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: mushboy] * 4
    #26022640 - 05/30/19 09:53 AM (4 years, 7 months ago)

Nice! This ... kinda makes a tek I was going to write up unnecessary! Good work :smile:


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: mushboy] * 2
    #26022675 - 05/30/19 10:07 AM (4 years, 7 months ago)

Quote:

mushboy said:
ooops.

post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people:shrug:



Haha, no oops, solid work here form a TC. I'll likely still post it, but it's been lowered in priority now :wink:


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Camera93] * 1
    #26022764 - 05/30/19 10:53 AM (4 years, 7 months ago)

Quote:

Camera93 said:
Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method



I've been messing around with Mudda bottles and grains, and let me just tell you that is NOT the route to go with them.

I started getting into LC simply because I want to keep doing mudda bottles for gourmet work and grains fucking suck with them. I have ideas on how to make grains work... but why when I can just pour in some LC. I'm a week or so out from my next test (I have 400ml of LC growing out right now, about 60% done), and I'll be pouring from media bottles (same as this tek essentially). I think, if anything, the small amount of extra hydration will be helpful for mudda bottles (the zip-lock twist kind).


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Camera93] * 1
    #26022817 - 05/30/19 11:37 AM (4 years, 7 months ago)

Ahhh, I realize I'm talking the same shit in two threads.
Quote:

Camera93 said:
I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir
that one has been the fastest by far.




Yeah, the dude from FreshCap Mushrooms shows using a wooden spoon handle to create a hole in the center before pc, then pouring into that hole:



This was my next idea... but still you can't shake/distribute it. I feel like the best option would be to do LC and the hole in the center, the give it a mean shake after you close the lid (then unscrew it a 1/4 turn for GE).


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Failboat] * 1
    #26046214 - 06/11/19 03:59 PM (4 years, 7 months ago)

Quote:

Quirkmeister92 said:
So just shake it? I've always failed with LC, might try again soon.



My success rate is much lower than I'd like, but I can make LC happen. Try with these lower nutes and fewer ingredients, it's a little easier to work with. I almost feel ready to start using tiny wedges to inoculate over a poke!


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: micelio] * 3
    #26046244 - 06/11/19 04:14 PM (4 years, 7 months ago)

I just swirl it like it's a wine glass. I'm half tempted to get magnetic stirrers and a stirring plate for no other reason than I think it would look bad ass and super science-y!

:superscience:


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Re: Making Liquid Culture [Re: micelio] * 1
    #26046302 - 06/11/19 04:37 PM (4 years, 7 months ago)

Quote:

micelio said:
I'm half tempted to get a glass of wine..Ha..



:hereyougo:


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: sandman420] * 1
    #26047588 - 06/12/19 08:28 AM (4 years, 7 months ago)

Quote:

sandman420 said:
Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.




I recently had a 75% colonized LC of 400ml, it hadn't really done anything in a while so I decided to unscrew the media bottle 1/4 turn in case I was suffocating it (I have a tendency to keep my unmodded lids a little on the tight side unfortunately due to paranoia). 3 days later I noticed mold around the inner rim :facepalm: It'd been growing out for almost 4 weeks w/o issue and one little 1/4 twist and BAM green.

:allsodisappointed:

Yeah, prickly whore is an understatement.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: micelio]
    #26047785 - 06/12/19 10:20 AM (4 years, 7 months ago)

Quote:

verum subsequentis said:
I wouldn't recommend unprotected lids for LC. If you want to use plastic lids for LC, wether modded or un, I would recommend wrapping twice or thrice with cling a para while still in the flow or sab.



That's a solid idea... shame I didn't think of it lol. I'm still very new at making LC, so these mistakes were totally expected. I have such little investment in this it's not funny. I have a jar of PE LC, my first that didn't contam. I've been letting it sit for almost a month now because I don't trust that it won't contam... I should probably pour it lol.

Quote:

micelio said:
Last night I found at Amazon a stirrer for 29.80 dollars with one magnetic stir bar and free shipping.
Now that's a decent price for someone to get started in stirring your LC.



That's been sitting in my cart for like a month now lol. I just need the $$$


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Dactylium] * 1
    #26050439 - 06/13/19 03:13 PM (4 years, 7 months ago)

Quote:

Dactylium said:
Quote:

verum subsequentis said:
@shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)




I wrap the threads of my jars several times with plumber's tape to form a nice seal with my plastic lids.



:breakthrough:

That stuff is good to PC? Damn, genius, I've always loved that shit.


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Dactylium]
    #26050580 - 06/13/19 04:32 PM (4 years, 7 months ago)

Quote:

Dactylium said:
(620F in freedom units)




LOOK YOU. :rofl: I just did a spit take from that. Literally. Good work friend :thumbup:


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Re: Making Liquid Culture [Re: sandman420]
    #26051816 - 06/14/19 07:43 AM (4 years, 7 months ago)

Wait, I thought it was plumbers tape on the media bottle threads...


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Re: Making Liquid Culture [Re: sandman420]
    #26051901 - 06/14/19 08:32 AM (4 years, 7 months ago)

Huh... I thought those weird caps (GL-45 or something like that?) were designed specifically to not create a 100% seal even in that case. Damn.

More on topic: I pulled the trigger on a stirplate and got 2 - 250ml media bottles... but I'm cheap and found the cheapest one. The fucking measure lines weren't even level and the damn drip-rim thing wouldn't stay on :facepalm: (you can see a gap in the image)



Returning them today, ordering other ones. I figure that if I'm going to do this I may as well do it right and get the right vessels. 500ml is just a little too big, these will be perfect and I can just PC them with the stir sticks in and trade off on the plate.

I think I'll stick with the cling-wrap over the cap and ignore the plumbers tape.

I also got that cheap ass stir plate, and it works but it's not great at low speeds (stalls out)... still. It works.


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Re: Making Liquid Culture [Re: sandman420]
    #26051916 - 06/14/19 08:40 AM (4 years, 7 months ago)

HUH! That must be nice. All of my media bottles are sans gaskets like this:



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Re: Making Liquid Culture [Re: SunnyDayze]
    #26133862 - 08/13/19 02:05 PM (4 years, 5 months ago)

So, most of these now aren't using stir plates... any reason why? I've noticed that with a stir plate I can get close to a full 200ml of usable LC within 3-5 days with a stir plate.

Also, I've been going dirty on my recipe. 200ml water + 2.5g LME + 1tsp (approx) dextrose. I get much faster results and the culture looks clear within about 3-5 days, but still brown and full of neutes.

Quote:

verum subsequentis said:
Flame sterilize a loop and dip it in the culture. [...] Just be sure that the only thing that goes in or over your open liquid has been flamed.




How in gods name would you flame sterilize the whole loop enough to make it "clean" to go over the top? Seriously, not trying to be annoying, I'd rather do this than waste a whole syringe on testing it out.




Edited by ShaperDreaming (08/13/19 02:09 PM)


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: LtLurker]
    #26133874 - 08/13/19 02:14 PM (4 years, 5 months ago)

Your answer is so simple...

:manofapproval:


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Re: Making Liquid Culture [Re: verum subsequentis]
    #26134028 - 08/13/19 03:52 PM (4 years, 5 months ago)

Honestly Verum, I'd feel pretty confidant with that mycelium island not being contam. I've let a couple go that ended up doing that, put them back on the stir plate and they went back under and turned out fine. My guess is that the myc prefers to not drown while it grows... this is very unscientific of me.

Though... when that happened it was a huge PITA to break up the myc and it got lodged in my syringe and... well, it eventually worked so :shrug: Still, not contaminated.

I'm excited to see that grow out.


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Re: Making Liquid Culture [Re: SunnyDayze]
    #26134062 - 08/13/19 04:09 PM (4 years, 5 months ago)

Looks like an agar puck dropped in there Sunny :thumbup:

Also, good point on the salt. Have I ever commented that every time I make grains I have the strongest desire to add salt and pepper? Every. Damn. Time. I once had to stop myself, I had the damn box of salt in my hand!


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Re: Making Liquid Culture [Re: El Chupacabra]
    #26136931 - 08/15/19 10:28 AM (4 years, 5 months ago)

How much beer do you add % wise? I have some skunky beer I've never thrown away in my fridge :rofl:

Does this just replace your LME, or do you also use that? I want a beer recipe because I like beer!


Edited by ShaperDreaming (08/15/19 10:29 AM)


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Re: Making Liquid Culture [Re: verum subsequentis]
    #26136970 - 08/15/19 10:54 AM (4 years, 5 months ago)

Verum, that's accurate. Here's from the OG Josex Poke Recipe:

Quote:

The standard ratio of LME to water is 1g LME for 500ml of water.
The broth will come out very clear using that ratio which is nice, but I personally prefer to pack in more nutrition when using LME, so I add 2 grams instead of 1. The broth will be darker but that's not a problem for me. You need to experiment to see which ratio you like.




I've made a few using this little neutes, but I tend to go for more. I do about 2.5g per 200ml now.

EDIT: My reason to up the neuts and getting a stir plate was that it took AGES for shit to grow in that recipe. You can still get very clear LC (like crystal clear) using 2.5g per 200ml, but it takes longer. With 2.5g LME + A splash of Dextrose I can get a fully colonized jar in 4-5 days on a stir plate.


Edited by ShaperDreaming (08/15/19 10:57 AM)


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