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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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Offlinemiha
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Registered: 09/20/03
Posts: 24
Loc: Slovenia
Last seen: 9 years, 1 month
Problem
    #2601325 - 04/25/04 04:08 PM (12 years, 7 months ago)

I got a shroom from a friend and made a print. His casing was  getting contaminated at the time. From then I have tryed PFtek several times and always got contaminated. Mycelium developes very nicely in jars but 3 days from birth they all get contaminated with cobweb. It starts spreading from the center where first mycelium formatted. In days jars get gray and later green.
Am I not paying enough attention to sterility or is the print contaminated and I can't do anything about it? Help. What can I do this is my only print :frown: . Help.thx


Edited by miha (04/25/04 04:09 PM)


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OfflineSuntzu
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Registered: 10/15/99
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Re: Problem [Re: miha]
    #2601412 - 04/25/04 04:39 PM (12 years, 7 months ago)

If a print is contaminated with mold, there is little that can be done to clean it up. Peroxide won't help much, because the spores won't germinate well. Antibiotics won't help much against mold. The best and only thing I can come up with involves agar work, where you streak out the print and try to transfer clean sections as soon as possible. This is a lot of work, considering a clean print can be obained from one of the fine shroomery vendors inexpensively. You may even be able to find a free one from many kind folks around here.
In short, if the print came from a mushroom growing off a moldy casing, and you're getting mold problems from that print. . .the number one thing to replace is the print itself.


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Offlinemiha
Stranger

Registered: 09/20/03
Posts: 24
Loc: Slovenia
Last seen: 9 years, 1 month
Re: Problem [Re: Suntzu]
    #2603689 - 04/26/04 05:35 AM (12 years, 7 months ago)

THX, I will get a new print.


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InvisibleAbuse
end of the line


Registered: 08/08/08
Posts: 6,039
Re: Problem [Re: miha]
    #16626358 - 08/01/12 05:04 AM (4 years, 4 months ago)

Quote:

miha said:
I got a shroom from a friend and made a print. His casing was  getting contaminated at the time. From then I have tryed PFtek several times and always got contaminated. Mycelium developes very nicely in jars but 3 days from birth they all get contaminated with cobweb. It starts spreading from the center where first mycelium formatted. In days jars get gray and later green.
Am I not paying enough attention to sterility or is the print contaminated and I can't do anything about it? Help. What can I do this is my only print :frown: . Help.thx




I'm going through this same problem, OP. BTW, you spelled 'tried' wrong.


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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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