This tek is part of bod's cultivation is easy as fuck link list


Bod's So Easy A Caveman Could Do It™ Spore Syringe Making TEK


First you need a spore print, If you don't have your own you can obtain spores from a vendor(see the sticky posts), or trading(in the marketplace also see sticky posts), or by making your own
Making your own spore print TEK

and you will need a spore syringe

I like to re-use ones I've gotten along the way. They work until they don't, it's that simple. the rubber wears out they're bad they last a long time though.

You also need a still air box.
My Still Air Box TEK
or a flow hood

One spore print can make many syringes
See this post for more information on that
Necessary reading on spores


Spore prints are not axenic(google this word if you don't know). Spore syringes are therefore not axenic. Do not panic about doing everything perfect. The shear amount of spores to contaminants will make sure you have an easy time using your syringe on PF cakes or on agar. Of course the more care you take the better quality you syringe will be, just don't go overboard.

Let's Get Started
before you start get your SAB ready, smoke a bowl, get your music shit figured out, and get your shot glass prepared. Take a shot and also sterilize a shot glass. I actually like to use a little dish because it's more spacious for doing multiple syringes in bulk or even just one syringe.




You can sterilize your shotglass/dish wrapped in foil in the oven
https://en.wikipedia.org/wiki/Dry_heat_sterilization
or also wrapped in foil in a pressure cooker at 15psi for 15 minutes.

once you have all this set

Boil some water on the stove. I use a skillet because it's faster



take your syringe and aspirate boiling water at least 3 times and squirt it out into your sink (not back into the pan!)

on second to last time I like to put the needle cap over the needle and squirt boiling water out of it to sterilize it as well. then fill the syringe again and put the cap on to keep the needle sterile. This way you can avoid using a torch for the entire process to sterilize the needle tip later.

Alternatively you could pressure cook your syringes to sterilize (15m at 15psi wrapped in foil) them but the boiling water method is sufficient and leaves them full of sterile water



put your syringe in the SAB and let it cool down for at least an hour (10cc syringes cool quickly bigger ones will take longer) or do this step the day before and let it cool down overnight.

once everything is cool and wont burn your spores proceed.

unwrap your print and hold it over your glass





squirting a few drops at a time and being extremely gentle with the needle tip dislodge spores into the shot glass.



aspirate spore water

once you get down to the last bits crinkle the foil so your glass is tilted (shot glasses need not do this step)




now you have a full syringe(or more)



Easier than a caveman way

you don't really need to sterilize a shot glass or dish



you can use the print as a dish, but I feel better using the sterilized dish to make syringes.



be gentile dislodging spores, and careful with the plunger on the syringe.

Bodhisatta said:

You can't buy strains of actives. You can only buy spores.
Spores have names those names are varieties
Any two spores that mate, make a strain.
If you grow from spores you have 1000s of strains in a single grow.
Some good some bad you will always get average unpredictable results from spores.
Spores don't know they have a name on their syringe. You could mix every variety together and there would be no competition because all the spores know is that they're cubensis and can mate with other cubes.

You can't buy a spore syringe for potency.
A spore syringe from me is just as good as one you can make yourself.
Potency is 100% related to your own skill doing culture work or pure luck.

Anything but spores of actives are illegal. So we cannot trade out cultures. If active mushrooms were legal they actually would sell strains. Vendors call varieties(names on a syringe) strains as a marketing strategy aimed at people who don't do any research first.

Spores don't know their variety. Any Psilocybe cubensis spore will mate with any other Psilocybe cubensis spore that has a compatible mating type.

When you grow from spores any two spores that successfully mate create a strain

A strain is not the same as a variety

When you grow from spores you have 100s if not 1000s of strains working together as a single organism.
You may choose any variety of psilocybe cubensis. Some strains will be weak some strong. When you grow from spores you get a mixed bag. If you have a B+ or GT syringe you could do 10 different grows and each would have different results.

If you want to get uniform results with consistent potency you need to keep good strains around by cloning or isolating and using agar. Spores will give you basically random results regardless of the variety you choose.
Strains Varieties and Species

Don't worry too much about which variety of psilocybe cubensis to pick. Chose one with a name you like and go with it. You'll be able to trade spores later. Mushrooms make their own spores so you never really need to buy more. Making a spore print is easier than it seems. Most noobs avoid making spore prints for whatever reason, you will need to learn this skill. The best way to learn is to start somewhere and make mistakes. Don't put all your eggs in one basket so that a mistake becomes horribly detrimental.

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Quote:

fastfred said:
Cubensis is heterothallic, tetrapolar, and bi-factorial.

Meaning that individual spores are not self-fertile and require mating with another spore to form a diploid individual.  Tetrapolar means that the initial diploid, dikaryon germ cell undergoes meiosis twice to form 4 haploid spores.  Bi-factorial means that there are two mating factors involved, which gives rise to 4 possible mating types from each individual.

There has been some confusion surrounding hetero vs homothallic.  In botany heterothallic is usually used to mean that the sexual structures occur in different plants.  In other types of biology it usually means that the sexual organs simply reside in separate structures, meaning that no individual structure is self-fertile.  So the meaning depends on what kingdom and species you are talking about.  That's why there has been some confusion.


-FF

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SunnyDayze said:

Great write up Bod, thanks!

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UncleHoyphae said:

Nice. A friend of mine wants to get into growing so I'm gonna try this method this evening n give him a few syringes to make mold with hehe. Thanks bod!

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UncleHoyphae said:

Nice. A friend of mine wants to get into growing so I'm gonna try this method this evening n give him a few syringes to make mold with hehe. Thanks bod!

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Yeah I just passed this off to a buddy of mine.

Neat, easy to follow instructional. Nice

bodhisatta said:

SunnyDayze said:

Great write up Bod, thanks!

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UncleHoyphae said:

Nice. A friend of mine wants to get into growing so I'm gonna try this method this evening n give him a few syringes to make mold with hehe. Thanks bod!

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glad to hear it. I bet he does better than mold though

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Kainin said:

Thank you for that, me being new to what's going on here it's great to see that there is a large community of people that are willing to help teach. I so many questions but with this site am leaning  exponentially do you have one question that I can't find an answer to you. how do I get my hand on spores? You mentioned "sticky post" sorry I'm still new,  can you point me in the right direction?thanks again for the insightful  walk-through

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bodhisatta said:

you can use the print as a dish, but I feel better using the sterilized dish to make syringes.

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thegoonking said:

Which tech do I need to follow to inoculate my oat jars? do I just use a sterile box and inject these spores directly?

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socalchef00 said:

This is awesome. Was wondering best way to do. simple and easy

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DERRAYLD said:

Not sure why he suggested squirting water at the print when you can just use the needle to dislodge some spores into the glass.

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Kinoko314 said:

That would be far from ideal.  Dry sterilization has to be done at something like 350-400F, and you probably want to hold that temp for about an hour.  IDK if the shot glass will survive.  Honestly you may be better off putting the shot glass in a pot of boiling water for like 20 minutes.  Of course if your air fryer is also a PC, then that would be the way to go.

Neither way is really ideal, and I haven't had a need to try either, so don't take what I say as gospel.

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