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Pinback
Stranger


Registered: 07/20/02
Posts: 837
Loc: Europe
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3475617 - 12/10/04 10:59 AM (20 years, 1 month ago) |
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Found this paper about ultrasonical preparation of mycelial suspensions when I searched this topic:
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themills
Posts do notindicateIntelligence

Registered: 07/24/04
Posts: 177
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Re: Breaking up clumps of spores in syringe [Re: Pinback]
#3482487 - 12/11/04 04:33 PM (20 years, 1 month ago) |
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So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better. Duh Duh (Law & Order sound)
-------------------- There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
_________________________________________________________________________________________________
Sibling Gatling Gun of Looking at All Sides of the Question
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: themills]
#3485274 - 12/12/04 04:54 AM (20 years, 1 month ago) |
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Quote:
themills said: So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better. Duh Duh (Law & Order sound)
yes, indeed. it works and well at that. and with sonicators going for $20-30 on ebay i think lots of folks could benefit from this tek.
-------------------- Admin @ mycotopia.net
Mycotopia
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3485798 - 12/12/04 11:01 AM (20 years, 1 month ago) |
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$20-30 is a sweet price and well within my range, I bet the wife would also love this for cleaning her rings and things! I really can't see this so much for spore dispersal so much but if this breaks up myc so well say in the jar this would indeed be an invaluable tool. I love toys
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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MushroomFriend
I smell a conspiracy!


Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 10 months, 4 days
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Re: Breaking up clumps of spores in syringe [Re: hyphae]
#3485860 - 12/12/04 11:14 AM (20 years, 1 month ago) |
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What is Jet dry used for, Im trying to find a local equivalent then.... It is no soap is it?
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 4 days, 10 hours
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Re: Breaking up clumps of spores in syringe [Re: MushroomFriend]
#3486043 - 12/12/04 12:09 PM (20 years, 1 month ago) |
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Dishwasher rinsing liquid.
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MushroomFriend
I smell a conspiracy!


Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 10 months, 4 days
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3486414 - 12/12/04 01:42 PM (20 years, 1 month ago) |
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Hmmz never use that, the supermarket might have it though. Thanks!
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: hyphae]
#3490146 - 12/13/04 08:02 AM (20 years, 1 month ago) |
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"if this breaks up myc so well say in the jar this would indeed be an invaluable tool."
yep. spores or tissue matters not to the sonicator. same principles in play. just tissue is more fragile so less time is needed.
-------------------- Admin @ mycotopia.net
Mycotopia
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3491841 - 12/13/04 02:53 PM (20 years, 1 month ago) |
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Hey hip, I've been wrong before!  this tek looks good to me. I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath? Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3496049 - 12/14/04 05:39 AM (20 years, 1 month ago) |
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Quote:
mycofile said: Hey hip, I've been wrong before!  this tek looks good to me. I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath? Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?
hmm, not yet but we'll get on it now. your thinking is it would speed growth by creating more points yes ?
-------------------- Admin @ mycotopia.net
Mycotopia
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3496059 - 12/14/04 05:42 AM (20 years, 1 month ago) |
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some new pix of sonicator in action on a old dry print



-------------------- Admin @ mycotopia.net
Mycotopia
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3498036 - 12/14/04 03:30 PM (20 years, 1 month ago) |
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My thinking is that it would create more points, yes, by breaking any clumps of spores up constantly. But also that the constant mixing might be helpful in some way. Most people just shake their jars every day or so, but lets remember that the pros use stirplates to constantly agitate their liquids. Perhaps these sonicators might offer some benefit as yet unknown?
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3502583 - 12/15/04 06:11 AM (20 years, 1 month ago) |
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i see where you're going. might have merit. but i'd imagine like most things there's a limit , perhaps a 2-3 second pulse once a day might be a good place to start testing. we can increase it from there if results are positive. i'll get our 'topiates to work on some new experiments asap. thx for the input.
-------------------- Admin @ mycotopia.net
Mycotopia
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sinfull7
Stranger

Registered: 12/15/04
Posts: 11
Last seen: 19 years, 10 months
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3507409 - 12/16/04 01:48 AM (20 years, 1 month ago) |
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there is also a Syringe needle That B&D makes .it filters particles down to .5 microns its the brown precision glides 1 1/2 in 18GA needles they sell them at almost every place that Ive got the regular pink needles. i use these to draw water into the syringe then just add a pink regular tip to draw up the spores. do you use a lot of spores in a typical syringe. because i only scrape the inoc loop on it twice. so mine are not that dark at all it just seems to help a lot . in the fact that i don't get a 100 sub-strains on one plate (just lets me see what i wanna grab a Lil better) . I've got black syringes from some vendors and even after diluting them 5 or more times its still dark . also i don't get any clumping using this ( its a spore dilution tech ) stamets method. am i alone in this because Ive noticed as of late vendors offering super DARK prints .
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: sinfull7]
#3507506 - 12/16/04 03:55 AM (20 years, 1 month ago) |
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I prefer an almost clear syringe.
My rule of thumb is, if on CLOSE inspection I can see a few..... THERE ARE PLENTY IN IT.
Frankly, a dark syringe is a waste of spores. All you need is enough to get the job done .
Enough to get the job done 1000 times over in a single syringe, just doesnt make sense . At least to frugal agar. 
As an example, this WBS jar contains one 0.5 CC squirt from an almost clear syringe.

This jar contains 1 CC in 2 squirts

This print contains about a million ++ spores.

If you are frugal, this one print could produce 100 VIABLE syringes.
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3507517 - 12/16/04 04:11 AM (20 years, 1 month ago) |
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actually i must be honest, i didnt read the whole thread
but heres a good way to break up clumps:
simply dismantle ur syringe and throw in a lil glasshed,
put it back together and autoclave.
once u drew up the syringe, prior to use u simply shake it around a bit
and the glasshed will do its job to mix up the solution quite properly.
u shouldnt worry too much about it plunding the needle,
since u simply hold it at an angle and all is good.
simple and works every time.
peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
Edited by ohmatic (12/16/04 04:28 AM)
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
#3507524 - 12/16/04 04:15 AM (20 years, 1 month ago) |
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A stainless steel BB works, to.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3507661 - 12/16/04 06:35 AM (20 years, 1 month ago) |
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the point isn't to make dark syringes, i'll agree. the ones in the pix above are far darker than needed, it's just an example. one could easily use much more water and dilute the concentration 10 fold and still get stellar success.
one advantage of the sonication method over adding a bb, etc. is that the sonic tek eliminates one more vector thru which contams might get in. no need to add anything, no bb, no jet dry, nadda. just water and spores. dilute to the desired concentration. as simple and as effective as one can possibly get.
-------------------- Admin @ mycotopia.net
Mycotopia
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3508644 - 12/16/04 12:27 PM (20 years, 1 month ago) |
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Quote:
Hippie3 said: one advantage of the sonication method over adding a bb, etc. is that the sonic tek eliminates one more vector thru which contams might get in.
well u tell me how contams are gonna get on a glass shed i place into a empty syringe prior to autoklaving please... once its toasted, there's not a single contam left, or do u know some contams withstanding a whopping 20 psi over 90 mins? peace ohm
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MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
#3515005 - 12/17/04 07:42 PM (20 years, 1 month ago) |
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there are quite a few spores that can survive a 'killing' heat, some wont germinate until that heat has been reached, there are also seeds that will only germinate after they have been burned, these high temp spores are something that many of us will never have to worry about but they do exist
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