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Zaphoid
Zaphoid

Registered: 03/29/04
Posts: 22
Loc: U.S.
Last seen: 19 years, 11 months
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Breaking up clumps of spores in syringe
#2599749 - 04/25/04 12:32 AM (20 years, 8 months ago) |
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A suggestion from a fellow shroomer. When he makes his own syringes he prints in a sterile jar with a couple of Stainless Steel 6-32 nuts in the jar. When loading the syringe these are shaken to break up the print. He also adds the same SS nut (1) to his syringes. The small SS nut is inserted by removing the plunger. The syringe is sterilized using the coffee cup of water in the microwave. The SS is inert and will not rust or affect the spores in any way. If the nut were to crush a few spores while it's breaking up the clumps so what. When you're making half a dozen syringes from one fresh print it ain't gonna matter. Then again it may not matter at all with all those fresh spores. Just thought I'd pass this along. I haven't heard of this before. Sorry if it's old news. I did a quick search and didn't find anything. Enjoy your shrooms!
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Gr0wer
always improving


Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2599773 - 04/25/04 12:44 AM (20 years, 8 months ago) |
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Ive thought abotu this only my idea was using a small glass ball or beador or a SS bearing. I wouldnt want a nut scratching up my syringe's insides. Only down side is you loose a cc or so when the nut or ball bottoms out, it would help allot with mixing though and give you more even growth between jars with the same syringe.
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zeta
Stranger

Registered: 05/24/02
Posts: 3,972
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2600100 - 04/25/04 04:12 AM (20 years, 8 months ago) |
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You shouldn't put steel in the microwave, and you can't sterilise things in the microwave anyway
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bobcat
XFL Talent Scout

Registered: 04/24/04
Posts: 8
Loc: Land of The Lost
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Re: Breaking up clumps of spores in syringe [Re: zeta]
#2600312 - 04/25/04 07:53 AM (20 years, 8 months ago) |
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you can steralize things in the microwave,but yiou shouldnt put metal in there.It will ruin your microwave.
-------------------- Get it in Writing!
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Suntzu
Geek


Registered: 10/14/99
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2601248 - 04/25/04 01:42 PM (20 years, 8 months ago) |
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Another option is to use a very tiny amount of detergent; It's easy to use too much, but a drop of liquid soap in about 100 mL water should work. I've only used tween out of convenience, so the amount may vary with other detergents. Properly diluted, the solution can be sterilized without foaming all over the place.
Perhaps triclosan or whatever antibiotic they put in soaps can help with dirty prints as well?
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: Suntzu]
#2604468 - 04/26/04 11:58 AM (20 years, 8 months ago) |
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Other than detergent, rinse aids for dishwashers are commonly used (aka jetdry).
In addition to that, I always liked to blend spore solutions in an eberbach (or it's diy version as detailed in 9er's tek or shroomdadi's mycomachine).
I am firmly convinced that PF used to blend his solutions as well as add an anti-clumping agent of some sort. This is based on his ambiguity in answering these questions when asked directly, as well as the equipment he was selling when he got popped. PF made the/some of the highest quality syringes ever marketed, if it was good enough for him it's good enough for the rest of us!
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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zooey
Stranger
Registered: 04/29/04
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2618702 - 04/29/04 08:54 PM (20 years, 8 months ago) |
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Go to a good jewelry store and buy an ultrasonic bath for cleaning rings (ultrasonics works by breaking the clumps of dirt into much smaller particles). Will work best if you use a glass vial w/rubber top or glass syringe full of spores as plastic tends to deaden ultrasonic waves. About 60 seconds of ultrasonics does nicely.
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Zaphoid
Zaphoid

Registered: 03/29/04
Posts: 22
Loc: U.S.
Last seen: 19 years, 11 months
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Re: Breaking up clumps of spores in syringe [Re: zooey]
#2651148 - 05/07/04 06:16 PM (20 years, 8 months ago) |
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Just came across this post again. You most certainly can sterilize in the microwave. He isn't putting the syringe in there. He heats up a coffee cup of water and then sucks up the boiling water in the syringe and spits it back out into the sink a couple of times. It's in one of the teks. He says he don't give a rats ass about losing a cc of spores because he's got plenty of prints to work with. The detergent and jet dry sounds kinda scary. I'd be afraid it would affect the spores. The ultrasonic device sounds like it would work great. His old lady is a jewlery freak. She probably has one of them. I'll let him know, Thanks!
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charvo
JOURNEYMAN


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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2654595 - 05/08/04 07:25 PM (20 years, 8 months ago) |
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i 've used jet dry many time it seems to have no negative affects on the sporse.it does however stop the sporse form clumping up and sticking to the rubber part of the plugger.
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Mykro_Guy
Myco Student


Registered: 05/08/04
Posts: 317
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2654789 - 05/08/04 08:42 PM (20 years, 8 months ago) |
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yea i dont like using the wave box
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: charvo]
#2657517 - 05/09/04 04:13 PM (20 years, 8 months ago) |
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I doubt the effectiveness of ultrasonic machines, but can not only personally vouch for jet-dry, I bet you've bought spores that were suspended in them from vendors before.
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#2658677 - 05/09/04 11:31 PM (20 years, 8 months ago) |
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Just an idea -- a .1% Tween-80 buffer might help, or, if you can't get this, any very mild detergent (surfactant -- SDS or maybe even electrasol -- who knows?)
-- Micro
-------------------- Any research paper or book for free
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: micro]
#2659683 - 05/10/04 01:31 PM (20 years, 8 months ago) |
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Quote:
Just an idea -- a .1% Tween-80 buffer might he
What is it, why would it be used (I assume it's a surfactant of sorts), where would one get it etc etc.
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#2661448 - 05/10/04 11:14 PM (20 years, 8 months ago) |
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Tween-80 is just a detergent (surfactant) and I'm sure it can't be controlled, so maybe somewhere online? It's used in biological solutions and I believe to help treat balding.
-- Micro
-------------------- Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3471119 - 12/09/04 02:35 PM (20 years, 1 month ago) |
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Quote:
mycofile said: I doubt the effectiveness of ultrasonic machines
well, don't. see http://www.mycotopia.net/discus/messages/2/175585.html?1102627771
-------------------- Admin @ mycotopia.net
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: zooey]
#3471253 - 12/09/04 03:02 PM (20 years, 1 month ago) |
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Quote:
zooey said: Go to a good jewelry store and buy an ultrasonic bath for cleaning rings (ultrasonics works by breaking the clumps of dirt into much smaller particles). Will work best if you use a glass vial w/rubber top or glass syringe full of spores as plastic tends to deaden ultrasonic waves. About 60 seconds of ultrasonics does nicely.
never saw your post until today but your suggestion works. i'd have given you credit if i had known. if you're still around, pm me & i'll be happy to give equal credit to you in our write-up. peace
-------------------- Admin @ mycotopia.net
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whiterasta
Day careobserver

Registered: 04/09/02
Posts: 1,780
Loc: Oregon
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Re: Breaking up clumps of spores in syringe [Re: Suntzu]
#3473882 - 12/09/04 11:10 PM (20 years, 1 month ago) |
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Triclosan is a marketing term for Chloroform. A sonicare toothbrush held against the syringe for a minute prior to use is all I've ever needed to break up and disperse spores nicely. It will ultrisonicly stir a plastic syringe and also keeps my teeth clean and white  WR
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Anno
Experimenter



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Re: Breaking up clumps of spores in syringe [Re: whiterasta]
#3474305 - 12/10/04 12:31 AM (20 years, 1 month ago) |
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Triclosan is a powder, chemically C12H7Cl3O. Chloroform is a liquid, chemically CHCl3.
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whiterasta
Day careobserver

Registered: 04/09/02
Posts: 1,780
Loc: Oregon
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3475189 - 12/10/04 07:54 AM (20 years, 1 month ago) |
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My bad Anno. Should've referenced the triclosan better. Still don't think I want it on my fungi. The toothbrush works well and I have been using it now for a year or so w/o dispersants. Now correct me here is Tween-80, polysorbate-80? WR
-------------------- To old for this place
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#3475226 - 12/10/04 08:18 AM (20 years, 1 month ago) |
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 This is very effective, cheap & easy to get & without negative effect after 3 month syringe storage in fridge & still viable. Toilet flush simple. Why do more?
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Pinback
Stranger


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Loc: Europe
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3475617 - 12/10/04 10:59 AM (20 years, 1 month ago) |
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Found this paper about ultrasonical preparation of mycelial suspensions when I searched this topic:
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themills
Posts do notindicateIntelligence

Registered: 07/24/04
Posts: 177
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Re: Breaking up clumps of spores in syringe [Re: Pinback]
#3482487 - 12/11/04 04:33 PM (20 years, 1 month ago) |
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So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better. Duh Duh (Law & Order sound)
-------------------- There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
_________________________________________________________________________________________________
Sibling Gatling Gun of Looking at All Sides of the Question
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Hippie3
mycotopiate


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Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: themills]
#3485274 - 12/12/04 04:54 AM (20 years, 1 month ago) |
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Quote:
themills said: So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better. Duh Duh (Law & Order sound)
yes, indeed. it works and well at that. and with sonicators going for $20-30 on ebay i think lots of folks could benefit from this tek.
-------------------- Admin @ mycotopia.net
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hyphae
born to grow


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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3485798 - 12/12/04 11:01 AM (20 years, 1 month ago) |
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$20-30 is a sweet price and well within my range, I bet the wife would also love this for cleaning her rings and things! I really can't see this so much for spore dispersal so much but if this breaks up myc so well say in the jar this would indeed be an invaluable tool. I love toys
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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MushroomFriend
I smell a conspiracy!


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Re: Breaking up clumps of spores in syringe [Re: hyphae]
#3485860 - 12/12/04 11:14 AM (20 years, 1 month ago) |
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What is Jet dry used for, Im trying to find a local equivalent then.... It is no soap is it?
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Anno
Experimenter



Registered: 06/17/99
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Re: Breaking up clumps of spores in syringe [Re: MushroomFriend]
#3486043 - 12/12/04 12:09 PM (20 years, 1 month ago) |
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Dishwasher rinsing liquid.
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MushroomFriend
I smell a conspiracy!


Registered: 10/12/04
Posts: 4,055
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3486414 - 12/12/04 01:42 PM (20 years, 1 month ago) |
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Hmmz never use that, the supermarket might have it though. Thanks!
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Hippie3
mycotopiate


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Posts: 3,090
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Re: Breaking up clumps of spores in syringe [Re: hyphae]
#3490146 - 12/13/04 08:02 AM (20 years, 1 month ago) |
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"if this breaks up myc so well say in the jar this would indeed be an invaluable tool."
yep. spores or tissue matters not to the sonicator. same principles in play. just tissue is more fragile so less time is needed.
-------------------- Admin @ mycotopia.net
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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3491841 - 12/13/04 02:53 PM (20 years, 1 month ago) |
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Hey hip, I've been wrong before!  this tek looks good to me. I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath? Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3496049 - 12/14/04 05:39 AM (20 years, 1 month ago) |
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Quote:
mycofile said: Hey hip, I've been wrong before!  this tek looks good to me. I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath? Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?
hmm, not yet but we'll get on it now. your thinking is it would speed growth by creating more points yes ?
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Hippie3
mycotopiate


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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3496059 - 12/14/04 05:42 AM (20 years, 1 month ago) |
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some new pix of sonicator in action on a old dry print



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mycofile
Pooh-Bah


Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3498036 - 12/14/04 03:30 PM (20 years, 1 month ago) |
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My thinking is that it would create more points, yes, by breaking any clumps of spores up constantly. But also that the constant mixing might be helpful in some way. Most people just shake their jars every day or so, but lets remember that the pros use stirplates to constantly agitate their liquids. Perhaps these sonicators might offer some benefit as yet unknown?
-------------------- "From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
#3502583 - 12/15/04 06:11 AM (20 years, 1 month ago) |
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i see where you're going. might have merit. but i'd imagine like most things there's a limit , perhaps a 2-3 second pulse once a day might be a good place to start testing. we can increase it from there if results are positive. i'll get our 'topiates to work on some new experiments asap. thx for the input.
-------------------- Admin @ mycotopia.net
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sinfull7
Stranger

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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3507409 - 12/16/04 01:48 AM (20 years, 1 month ago) |
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there is also a Syringe needle That B&D makes .it filters particles down to .5 microns its the brown precision glides 1 1/2 in 18GA needles they sell them at almost every place that Ive got the regular pink needles. i use these to draw water into the syringe then just add a pink regular tip to draw up the spores. do you use a lot of spores in a typical syringe. because i only scrape the inoc loop on it twice. so mine are not that dark at all it just seems to help a lot . in the fact that i don't get a 100 sub-strains on one plate (just lets me see what i wanna grab a Lil better) . I've got black syringes from some vendors and even after diluting them 5 or more times its still dark . also i don't get any clumping using this ( its a spore dilution tech ) stamets method. am i alone in this because Ive noticed as of late vendors offering super DARK prints .
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: sinfull7]
#3507506 - 12/16/04 03:55 AM (20 years, 1 month ago) |
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I prefer an almost clear syringe.
My rule of thumb is, if on CLOSE inspection I can see a few..... THERE ARE PLENTY IN IT.
Frankly, a dark syringe is a waste of spores. All you need is enough to get the job done .
Enough to get the job done 1000 times over in a single syringe, just doesnt make sense . At least to frugal agar. 
As an example, this WBS jar contains one 0.5 CC squirt from an almost clear syringe.

This jar contains 1 CC in 2 squirts

This print contains about a million ++ spores.

If you are frugal, this one print could produce 100 VIABLE syringes.
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ohmatic
searcher


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Re: Breaking up clumps of spores in syringe [Re: agar]
#3507517 - 12/16/04 04:11 AM (20 years, 1 month ago) |
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actually i must be honest, i didnt read the whole thread
but heres a good way to break up clumps:
simply dismantle ur syringe and throw in a lil glasshed,
put it back together and autoclave.
once u drew up the syringe, prior to use u simply shake it around a bit
and the glasshed will do its job to mix up the solution quite properly.
u shouldnt worry too much about it plunding the needle,
since u simply hold it at an angle and all is good.
simple and works every time.
peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
Edited by ohmatic (12/16/04 04:28 AM)
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
#3507524 - 12/16/04 04:15 AM (20 years, 1 month ago) |
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A stainless steel BB works, to.
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3507661 - 12/16/04 06:35 AM (20 years, 1 month ago) |
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the point isn't to make dark syringes, i'll agree. the ones in the pix above are far darker than needed, it's just an example. one could easily use much more water and dilute the concentration 10 fold and still get stellar success.
one advantage of the sonication method over adding a bb, etc. is that the sonic tek eliminates one more vector thru which contams might get in. no need to add anything, no bb, no jet dry, nadda. just water and spores. dilute to the desired concentration. as simple and as effective as one can possibly get.
-------------------- Admin @ mycotopia.net
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3508644 - 12/16/04 12:27 PM (20 years, 1 month ago) |
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Quote:
Hippie3 said: one advantage of the sonication method over adding a bb, etc. is that the sonic tek eliminates one more vector thru which contams might get in.
well u tell me how contams are gonna get on a glass shed i place into a empty syringe prior to autoklaving please... once its toasted, there's not a single contam left, or do u know some contams withstanding a whopping 20 psi over 90 mins? peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
#3515005 - 12/17/04 07:42 PM (20 years, 1 month ago) |
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there are quite a few spores that can survive a 'killing' heat, some wont germinate until that heat has been reached, there are also seeds that will only germinate after they have been burned, these high temp spores are something that many of us will never have to worry about but they do exist
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: Prisoner#1]
#3515284 - 12/17/04 09:43 PM (20 years, 1 month ago) |
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Quote:
Prisoner#1 said: there are quite a few spores that can survive a 'killing' heat, some wont germinate until that heat has been reached, there are also seeds that will only germinate after they have been burned, these high temp spores are something that many of us will never have to worry about but they do exist
so, just to make my point clear. if i prepare a syringe with a glass shed inside, as stated above - then autoklave this syringe at 20 or even 25psi for 60mins,there wont be ANYTHING left bad and alive on it from my point of undestandment.
even, if the normal pressure cooker, running at 15psi, the same syringe with the same glasshed, will be sterile if pc'd for 60mins. prolly even shorter.
anyway, u get my point. i know, that for example, endospores on rye, can survive pcing if the rye isnt presoaked.
does the same factor apply to whatever may be on the syringe/glass shed and does this actually MATTER for hobbiest? peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
#3516180 - 12/18/04 02:29 AM (20 years, 1 month ago) |
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this issue is so not advanced...
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micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Breaking up clumps of spores in syringe [Re: Zen Peddler]
#3519326 - 12/19/04 01:07 AM (20 years, 1 month ago) |
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Buy a vortex. They go for like $20 on labx.xom
-------------------- Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: Zen Peddler]
#3556442 - 12/29/04 07:35 AM (20 years, 22 days ago) |
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Quote:
bluemeanie said:
this issue is so not advanced...
lol
i think perhaps
you just aren't 'advanced' enough in your thinking
to recognize potential when it's right in front of you.
but you're not alone in that.
kinda funny how just about everytime
something new & exciting comes along
lots of nay-sayers try to kill it fast.
first they delete the thread making the announcement,
so i tack my results on to this old thread.
suddenly a thread which had been in Advanced for almost EIGHT months
is no longer 'suitable'
and so they kick it down to here
where they hope fewer people will see it.
why ?
because it pains them to no end
that they didn't think of it,
but i did.
now here's some more
photo proof of just WHY
this ultrasonic method
is far better than anything we've used before.
from ultrasonics for spore/tissue suspension?
Troutlips sez-
Quote:
made up 12, half pint PF jars.I inoculated three groups of four jars with different syringes.
The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs., added water to the print and sat the jar on my ultrasonic humidifier for about an hour.These were B+.
On the fourth day the ultrasonic group were showing impressive growth while the other two groups showed little or no visible growth.
jars now 8 days old
as anyone [even those not-very-advanced] can plainly see,
the syringe exposed to the ultrasound
is producing extraordinary fast growth
compared to ordinary spore syringes
and even faster
than liquid culture [karo],
which supposedly is fastest of all.
-------------------- Admin @ mycotopia.net
Mycotopia
Edited by Hippie3 (12/30/04 03:53 AM)
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ticktock
Seeker


Registered: 10/02/04
Posts: 389
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3556756 - 12/29/04 09:54 AM (20 years, 22 days ago) |
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To break up clumps in a syringe; Heat needle to red in flame of alcohol lamp. With the tip still in the flame, draw 1 ml of hot air. The air space in the barrel allows some serious sloshing. Works for me.
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Don't panic!
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: ticktock]
#3560331 - 12/30/04 03:26 AM (20 years, 21 days ago) |
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another nice tip,
but this sonic method is still different
and i think 'better'
as no air is drawn in
hence less risk of introducing contams
but to each his own.
-------------------- Admin @ mycotopia.net
Mycotopia
Edited by Hippie3 (12/30/04 03:52 AM)
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boxtop703
ThesaurusLinguae Graecae


Registered: 06/26/01
Posts: 5,011
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3560373 - 12/30/04 03:55 AM (20 years, 21 days ago) |
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Quote:
Hippie3 said: does that give you nearly full colonization in just 4 days ? don't think so...
This looks valid and I'm going to begin incorporating this into the TLG syringe making process I do believe.
For more thoroughly dispersed microscopy samples of course.
It is very simple but who said an idea had to be complicated to be effective so while it may or may not be "advanced", it most certainly appears to be an advancement.
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3560835 - 12/30/04 09:57 AM (20 years, 21 days ago) |
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Quote:
The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.
While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.
If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing.
Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3561043 - 12/30/04 11:13 AM (20 years, 21 days ago) |
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Quote:
Anno said:
Quote:
The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.
While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.
If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing. Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.
Exactly what I was going to say. I'm gratful for the info and will end up with one of these (especially for liquid cultures..sounds very interesting) but I think a better experiment should be done...at least a control (unshaken, same spores) should have been used.
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troutlips
Stranger
Registered: 02/27/04
Posts: 8
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3562972 - 12/30/04 08:32 PM (20 years, 21 days ago) |
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You are right, Anno. I was not really trying to prove anything with that batch of three different strains,but when I noticed the result, I posted it in a similar thread to this one at 'Topia. Hippie just thought that it demonstrated the ultra sonic dispersal tek pretty well and posted it here. I was just trying it out for myself,not by any means a scientific experiment. I was just tryin' to grow some shrooms! But you are right. Next batch I build, I will do as you suggest and use the same print prepared in the three methods you mentioned above. Peace.
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djred
newbie

Registered: 08/27/04
Posts: 922
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Re: Breaking up clumps of spores in syringe [Re: troutlips]
#3563852 - 12/31/04 12:06 AM (20 years, 21 days ago) |
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i read this whole thread..well most of what seemed of use..still tick tock is the only one who i found to give results..somehow it wen tfomr syringes to ultra sonic jewlery cleaner..to somehting else..tlg is the bomb i still haven't broke up that syringe like it was the day i got it..hopefully i broke it up enough..it was 1 huge clump until i beat the shit out of it for 30 minutes..
thinking enough spores got jarred loose for success hopefully..?even though some clumps hang around?
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Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3583037 - 01/05/05 05:22 AM (20 years, 15 days ago) |
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Quote:
Anno said:
Quote:
The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.
While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.
If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing. Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.
people do this work in real life, with what they have on hand. while it might not meet all rigorous requirements of a truly 'scientific' study nevertheless anyone can see the very obvious difference in growth. that is a valid observation even if the methodology is flawed. now find something else to criticize, nit-pick, etc. if one doesn't create i guess all that's left is to destroy. have fun.
-------------------- Admin @ mycotopia.net
Mycotopia
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 4 days, 10 hours
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3583046 - 01/05/05 05:28 AM (20 years, 15 days ago) |
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>anyone can see the very obvious difference in growth. >that is a valid observation >even if the methodology is flawed.
Sure there is a difference in growth.
But to draw the conclusions you draw from it, is more than flawed. I am glad to see everybody (but you) realizes this.
>now find something else to criticize, nit-pick, etc.
Keep your hostile attitude at mycotopia, thank you.
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
#3583048 - 01/05/05 05:30 AM (20 years, 15 days ago) |
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well i stick to sterilizing my syringes with a little glass shed inside which is a super handy helper once the syringes are prepared since it breaks apart spores in no time.
no need for all this fancy super duper stuff  even the standard air bubble in the syring helps, the shed is even better. peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 12 years, 14 days
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
#3584624 - 01/05/05 03:10 PM (20 years, 15 days ago) |
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You put a whole shed in your syringe? And where do you get glass sheds? I've seen wooden ones and metal ones but I dont think I've ever seen a glass shed. It wouldnt be very good for keeping people away from your tools or lawnmower or anything. Plus I still think it would be difficult to put a whole shed into any of the syringes I have.
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 4 days, 10 hours
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
#3588096 - 01/06/05 08:26 AM (20 years, 14 days ago) |
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It is difficult, but it is possible
Well, here are the images from a test done at mycotopia like it is supposed to be done.
Syringes prepared from the same spore print , one syringe treated with ultrasonic, one not.
Would you call this a "very obvious difference" in growth? Or even "dramatic" ?
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 4 days, 10 hours
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
#3588107 - 01/06/05 08:30 AM (20 years, 14 days ago) |
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Quote:
scatmanrav said: And where do you get glass sheds?
You find them in the woods, of course!
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3588164 - 01/06/05 08:44 AM (20 years, 14 days ago) |
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Quote:
Anno said:
Quote:
scatmanrav said: And where do you get glass sheds?
You find them in the woods, of course!
 gotta love anno's humor 
peace ohm
--------------------
MONOTUB tek HEATBOMB tek
RIP #cultivation! ....can't associate? well FUCK U !
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3588199 - 01/06/05 08:52 AM (20 years, 14 days ago) |
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Cool.
 couldn't you just expell the whole syringe into a sterile container and draw it back inside to break up the clumps? And as for liquid cultures growing in ultrasound...try sticking your finger in an ultrasound bath!Would you feel like breeding in there? LOL sorry I don't mean to laugh but it's a favorite trick of mine to play on people"You gotta feel this,it's so wierd!" I have an ultrasonic mist maker that I use for certain things(ahem!) I will be adding "spore shaker"to the list though,nice pics,I'm convinced. I make prints on strips of spawnbag then throw them right into the syringe.
 This will really shake them loose!
Thanks!
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 12 years, 14 days
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3612958 - 01/11/05 01:41 PM (20 years, 9 days ago) |
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Quote:
Anno said:
Quote:
scatmanrav said: And where do you get glass sheds?
You find them in the woods, of course!
Ok but now I'm stuck finding syringes that the shed will fit into...thats a pretty big shed too.....
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
#3613054 - 01/11/05 02:07 PM (20 years, 9 days ago) |
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My foaf has a syringe it will fit in for $1 (no COD).
$987,734 for S&H . Cash only.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
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Re: Breaking up clumps of spores in syringe [Re: agar]
#3613079 - 01/11/05 02:17 PM (20 years, 9 days ago) |
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That damn well better come with a needle to fit....
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
#3613128 - 01/11/05 02:29 PM (20 years, 9 days ago) |
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Needle is only 0.25cents extra. Needle S&H is $79,431
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 4 days, 10 hours
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Re: Breaking up clumps of spores in syringe [Re: Peterthinks]
#3622526 - 01/13/05 07:46 AM (20 years, 7 days ago) |
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>I will be adding "spore shaker"to the list though,nice pics,I'm convinced.
Convinced? Of what? Take a look at the pictures again. There is virtually no difference at all between the growth inoculated with the ultrasonically threaded syringes and the normal syringes.
Much hype, not much better.
Here are the images from a test done at mycotopia.
Syringes prepared from the same spore print , one syringe was treated with ultrasonic, one not.
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boxtop703
ThesaurusLinguae Graecae


Registered: 06/26/01
Posts: 5,011
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Re: Breaking up clumps of spores in syringe [Re: Anno]
#3622657 - 01/13/05 08:51 AM (20 years, 7 days ago) |
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I see a clear difference between the two and it's too bad that a spore dispersal agent wasn't added into the mix.
Ultrasonic by itself is just limited to breaking up the spores, it's the dispersal agent that keep the spores suspended that makes for the real magic under the scope.
You would then notice a huge difference I do believe.
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MushroomFriend
I smell a conspiracy!


Registered: 10/12/04
Posts: 4,055
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Re: Breaking up clumps of spores in syringe [Re: boxtop703]
#3622882 - 01/13/05 10:37 AM (20 years, 7 days ago) |
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I think that importance of preventing, and breaking up, sporeclumps is overrated. In a fat purple syringe between the clumps there still are plenty of thousands of not millions of spores after a hefty shake.
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boxtop703
ThesaurusLinguae Graecae


Registered: 06/26/01
Posts: 5,011
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Re: Breaking up clumps of spores in syringe [Re: boxtop703]
#3623600 - 01/13/05 01:59 PM (20 years, 7 days ago) |
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And if anyone's looking, here's a nice digital Branson going fairly cheap on Ebay:
http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&category=494&item=4957547815&rd=1
It has gone up $30 since this morning though.
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