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OfflineZaphoid
Zaphoid

Registered: 03/30/04
Posts: 22
Loc: U.S.
Last seen: 12 years, 7 months
Breaking up clumps of spores in syringe
    #2599749 - 04/25/04 02:32 AM (13 years, 5 months ago)

A suggestion from a fellow shroomer. When he makes his own syringes he prints in a sterile jar with a couple of Stainless Steel 6-32 nuts in the jar. When loading the syringe these are shaken to break up the print. He also adds the same SS nut (1) to his syringes. The small SS nut is inserted by removing the plunger. The syringe is sterilized using the coffee cup of water in the microwave. The SS is inert and will not rust or affect the spores in any way. If the nut were to crush a few spores while it's breaking up the clumps so what. When you're making half a dozen syringes from one fresh print it ain't gonna matter. Then again it may not matter at all with all those fresh spores. Just thought I'd pass this along. I haven't heard of this before. Sorry if it's old news. I did a quick search and didn't find anything. Enjoy your shrooms!  :cool:


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God? My imaginary friend's name is Bobo!

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OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,035
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2599773 - 04/25/04 02:44 AM (13 years, 5 months ago)

Ive thought abotu this only my idea was using a small glass ball or beador or a SS bearing. I wouldnt want a nut scratching up my syringe's insides. Only down side is you loose a cc or so when the nut or ball bottoms out, it would help allot with mixing though and give you more even growth between jars with the same syringe.


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Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2600100 - 04/25/04 06:12 AM (13 years, 5 months ago)

You shouldn't put steel in the microwave, and you can't sterilise things in the microwave anyway


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Invisiblebobcat
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Registered: 04/24/04
Posts: 8
Loc: Land of The Lost
Re: Breaking up clumps of spores in syringe [Re: zeta]
    #2600312 - 04/25/04 09:53 AM (13 years, 5 months ago)

you can steralize things in the microwave,but yiou shouldnt put metal in there.It will ruin your microwave.


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OfflineSuntzu
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Registered: 10/15/99
Posts: 1,394
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2601248 - 04/25/04 03:42 PM (13 years, 5 months ago)

Another option is to use a very tiny amount of detergent; It's easy to use too much, but a drop of liquid soap in about 100 mL water should work. I've only used tween out of convenience, so the amount may vary with other detergents. Properly diluted, the solution can be sterilized without foaming all over the place.

Perhaps triclosan or whatever antibiotic they put in soaps can help with dirty prints as well?


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
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Re: Breaking up clumps of spores in syringe [Re: Suntzu]
    #2604468 - 04/26/04 01:58 PM (13 years, 4 months ago)

Other than detergent, rinse aids for dishwashers are commonly used (aka jetdry).

In addition to that, I always liked to blend spore solutions in an eberbach (or it's diy version as detailed in 9er's tek or shroomdadi's mycomachine).

I am firmly convinced that PF used to blend his solutions as well as add an anti-clumping agent of some sort. This is based on his ambiguity in answering these questions when asked directly, as well as the equipment he was selling when he got popped. PF made the/some of the highest quality syringes ever marketed, if it was good enough for him it's good enough for the rest of us!


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Offlinezooey
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Registered: 04/29/04
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Last seen: 13 years, 4 months
Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2618702 - 04/29/04 10:54 PM (13 years, 4 months ago)

Go to a good jewelry store and buy an ultrasonic bath for cleaning rings (ultrasonics works by breaking the clumps of dirt into much smaller particles). Will work best if you use a glass vial w/rubber top or glass syringe full of spores as plastic tends to deaden ultrasonic waves. About 60 seconds of ultrasonics does nicely.


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OfflineZaphoid
Zaphoid

Registered: 03/30/04
Posts: 22
Loc: U.S.
Last seen: 12 years, 7 months
Re: Breaking up clumps of spores in syringe [Re: zooey]
    #2651148 - 05/07/04 08:16 PM (13 years, 4 months ago)

Just came across this post again. You most certainly can sterilize in the microwave. He isn't putting the syringe in there. He heats up a coffee cup of water and then sucks up the boiling water in the syringe and spits it back out into the sink a couple of times. It's in one of the teks. He says he don't give a rats ass about losing a cc of spores because he's got plenty of prints to work with. The detergent and jet dry sounds kinda scary. I'd be afraid it would affect the spores. The ultrasonic device sounds like it would work great. His old lady is a jewlery freak. She probably has one of them. I'll let him know, Thanks!


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God? My imaginary friend's name is Bobo!

[/image]http://www.shroomery.org/forums/files/04-19/413223381-thumb_10_9_1341.gif[/image]


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Offlinecharvo
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Registered: 03/09/03
Posts: 654
Loc: In The Hand Of The World
Last seen: 3 years, 4 months
Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2654595 - 05/08/04 09:25 PM (13 years, 4 months ago)

i 've used jet dry many time it seems to have no negative affects on the sporse.it does however stop the sporse form clumping up and sticking to the rubber part of the plugger.


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InvisibleMykro_Guy
Myco Student
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Registered: 05/08/04
Posts: 317
Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2654789 - 05/08/04 10:42 PM (13 years, 4 months ago)

yea i dont like using the wave box


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: charvo]
    #2657517 - 05/09/04 06:13 PM (13 years, 4 months ago)

I doubt the effectiveness of ultrasonic machines, but can not only personally vouch for jet-dry, I bet you've bought spores that were suspended in them from vendors before.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #2658677 - 05/10/04 01:31 AM (13 years, 4 months ago)

Just an idea -- a .1% Tween-80 buffer might help, or, if you can't get this, any very mild detergent (surfactant -- SDS or maybe even electrasol -- who knows?)

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Invisiblemycofile
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Loc: Uranus
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Re: Breaking up clumps of spores in syringe [Re: micro]
    #2659683 - 05/10/04 03:31 PM (13 years, 4 months ago)

Quote:

Just an idea -- a .1% Tween-80 buffer might he



What is it, why would it be used (I assume it's a surfactant of sorts), where would one get it etc etc.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Breaking up clumps of spores in syringe [Re: mycofile]
    #2661448 - 05/11/04 01:14 AM (13 years, 4 months ago)

Tween-80 is just a detergent (surfactant) and I'm sure it can't be controlled, so maybe somewhere online? It's used in biological solutions and I believe to help treat balding.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleHippie3
mycotopiate
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Registered: 11/07/99
Posts: 3,090
Loc: mycotopia.net
Re: Breaking up clumps of spores in syringe [Re: mycofile]
    #3471119 - 12/09/04 04:35 PM (12 years, 9 months ago)

Quote:

mycofile said:
I doubt the effectiveness of ultrasonic machines




well,
don't.
see http://www.mycotopia.net/discus/messages/2/175585.html?1102627771


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Admin @ mycotopia.net
Mycotopia


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InvisibleHippie3
mycotopiate
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Registered: 11/07/99
Posts: 3,090
Loc: mycotopia.net
Re: Breaking up clumps of spores in syringe [Re: zooey]
    #3471253 - 12/09/04 05:02 PM (12 years, 9 months ago)

Quote:

zooey said:
Go to a good jewelry store and buy an ultrasonic bath for cleaning rings (ultrasonics works by breaking the clumps of dirt into much smaller particles). Will work best if you use a glass vial w/rubber top or glass syringe full of spores as plastic tends to deaden ultrasonic waves. About 60 seconds of ultrasonics does nicely.




never saw your post until today
but your suggestion works.
i'd have given you credit
if i had known.
if you're still around,
pm me & i'll be happy to give equal credit
to you in our write-up.
peace


--------------------
Admin @ mycotopia.net
Mycotopia


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Invisiblewhiterasta
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Registered: 04/09/02
Posts: 1,780
Loc: Oregon
Re: Breaking up clumps of spores in syringe [Re: Suntzu]
    #3473882 - 12/10/04 01:10 AM (12 years, 9 months ago)

Triclosan is a marketing term for Chloroform.
A sonicare toothbrush held against the syringe for a minute prior to use is all I've ever needed to break up and disperse spores nicely. It will ultrisonicly stir a plastic syringe and also keeps my teeth clean and white :lol:
WR:wexican:


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To old for this place


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OfflineAnnoA
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Registered: 06/18/99
Posts: 24,123
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Last seen: 2 months, 27 days
Re: Breaking up clumps of spores in syringe [Re: whiterasta]
    #3474305 - 12/10/04 02:31 AM (12 years, 9 months ago)

Triclosan is a powder, chemically C12H7Cl3O.
Chloroform is a liquid, chemically CHCl3.


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Invisiblewhiterasta
Day careobserver
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Registered: 04/09/02
Posts: 1,780
Loc: Oregon
Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3475189 - 12/10/04 09:54 AM (12 years, 9 months ago)

My bad Anno. Should've referenced the triclosan better. Still don't think I want it on my fungi.
The toothbrush works well and I have been using it now for a year or so w/o dispersants.
Now correct me here is Tween-80, polysorbate-80?
WR:wexican:


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To old for this place


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Invisibleagar
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Registered: 11/21/04
Posts: 9,056
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #3475226 - 12/10/04 10:18 AM (12 years, 9 months ago)


This is very effective, cheap & easy to get & without negative effect after 3 month syringe storage in fridge & still viable. Toilet flush simple. Why do more?


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