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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Re: Cordyceps Militaris [Re: bebop]
    #26965938 -

Does anybody have any advice on the above? Sorry to annoy, I can expand the LC to more LC today if my agar mixture will work, or I can order the stuff from jake's recipe - just don't want to waste my LC.

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Re: Cordyceps Militaris [Re: bebop] * 1
    #26966023 -

I believe the culture itself will grow in any standard agar or LC recipe for expansion, it's just for fruiting that you need all those other additives.

And no you don't need to use your whole 10ml syringe to start a pint of LC, just use 1ml and save the syringe you got as a backup.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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Re: Cordyceps Militaris [Re: bebop] * 1
    #26966026 -

Sorry man it’s been a crazy week yeah you can substitute other sugars there are tons of recipes that will work this will just give you a starting point and a base idea but yeah man use what you have and see what works don’t go too far from the path but supplementing one sugar for another won’t be a problem


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JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #26966074 -

Perfect! Thank you both. I'll make a standard LC with corn syrup & use 1-2 tsp of my agar mix for the broth. :cool:

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Re: Cordyceps Militaris [Re: bebop]
    #26985698 -

experimenting with these now.. just loaded tons of jars into a tent.

i have brown rice, but it would be way more local for me to use rye.  From what i can determine, a major difference between brown rice nutrient and rye is the bran contained with the rice.  Instead of hot loading from rice cooker, i about to pressure cook rye bags which are supplemented with bran.. inoculate cordycep lc into bag, and once colonized load them onto monotubs.

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Re: Cordyceps Militaris [Re: trippleblack]
    #26988121 -

I had better luck doing bulk tubs with bags and pressure cooker....I only had good luck using the instapot for deli containers


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Where am I at?


[/url]
“To be godless is probably the first step to innocence," he said, "to lose the sense of sin and subordination, the false grief for things supposed to be lost."
So by innocence you mean not an absence of experience, but an absence of illusions."
An absence of need for illusions," he said. "A love of and respect for what is right before your eyes.”― Anne Rice, The Vampire Lestat

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #27201074 -

hi, im searching around for some cordyceps teks, is your recipe and rice/broth ratio still up to date?

i got some LC thats probebly ready in a couple of days

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Re: Cordyceps Militaris [Re: Boile]
    #27225524 -

Hi!

I am also gathering all the info I can while waiting for my cordyceps mycelium transfers to grow on the agar plates.
It is doing well and I will soon go for the fruiting step.
I want to experiment with different fruiting substrates, but the problem is that I am not sure to be able to find all the supplements.
I could get some vitamine B1 and ordered peptone and K2HPO4 that are on the way.
But other chemicals and azomite will be much more difficult to find here in Spain.
Meanwhile, I wonder if I could use a more natural substrate with good results. I can buy some dehydrated silkworm pupae, and my question is if they could provide all the nutrients that the chemicals do.
So, would it be a good idea to mix 50% whole rice + 50% silkworm pupae?
Am I missing any important supplement here?
My aim is that the fruits have as many medicinal properties as possible.

Thanks!

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Re: Cordyceps Militaris [Re: karloff]
    #27253412 -

Ok, finally I could find all the ingredients but the ammonium citrate (but it is on the way for my next culture).
So I made 7 jars for fruiting the plates I had. The plates were classic MEA where I put the original cordyceps tissue (from a specimen found in the wild that was sporulating, so it is mostly a MS culture). I got nice mycelium and did up to T2 transfers.
My plan is to try getting some fruits and use the best specimen to make a clone and use it for the main culture, with more jars, but now I have a doubt... does it really work making clones and growing them or do I need to cross more than one single specimen?
Another questions that have arised in the proccess:

-Following the original formula (30 gr. brown rice + 44 gr. nutrient solution in each jar) I found that, after pressure cooking, the rice grains were not loose, but in a compact cake that is impossible to break as much as I hit the jar. Is this normal?

-I am not doing a LC at this stage, just putting a wedge into the fruiting jars. Is it ok or will it have a lack in the ammount of liquid?

-In three of the jars I have added 5 gr. of crushed dry silkworm pupae, just for experimenting. Has anyone tried this kind of supplement with good results?

That's all for now. Thanks for your help

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Re: Cordyceps Militaris [Re: karloff]
    #27260596 -

I'm getting cordys soon...Wanted to ask if when you spore to plates if they need to be kept in the dark or not? Obviously, they'll be in the light while doing the flow work.

Awesome tek and thanks for putting this together!!


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"Never miss a good chance to shut up"
                Best advice ever...in this hobby

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Re: Cordyceps Militaris [Re: ShroomyToons]
    #27262840 -

There are lots of broth recipes that will work you do not have to follow mine exactly I change mine around slightly all the time looking for improvements I haven’t ran these in a few months but I am about to gear up a very large grow of them I will keep this thread updated with my progress


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JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #27262851 -

Why couldn't you colonize in a filter patch bag, or a silicon dough bag like mushroom revival uses to sterilize there mixture?

Could you spawn colonized substrate to a SANITIZED container?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
 DMT for IandI

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Re: Cordyceps Militaris [Re: QM33]
    #27262861 -

I’ve tried using bags before however in my experience cordyceps culture really does not respond well to being disturbed none of the bags I ran fruited... Maybe if you never touched the bag after inoculating it would work


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JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #27262869 -

What do you mean distributed? Like colonized, broken up then left to reconsolidate? Or colonized, broken up with addition new substrate, then left to recolonize.
You mean not touching the bag after inoculating like, mix the sub with LC in the bag, then just leave it completely? As compared to what?
And potentially just fruit in the bag? I was thinking of trying to lay them long ways also.

Ide like to make some culture in bags, inoculate, get spawn then just lay it down in a tub. You don't think so?
Rather than sterilizing a bunch of containers with the sub in it.
Maybe colonize a bag of sub then break it into a few containers or one big one that has just been sanitized, rather than sterilized, porltentially saving alot of room/time/energy. Cords cube style?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
 DMT for IandI

Extras: Filter Print Post Top
Re: Cordyceps Militaris [Re: QM33]
    #27262873 -

Like all of it, once you inoculate it you do not want to disturb the Grain at all it sits in total darkness for two weeks afterwords it is fully colonized and you start the light cycle you definitely can’t break it apart after the dark cycle put it in a new container and then fruit it, it will not work. even just doing it in a bag and then picking the bag up out of your dark closet to move to your fruiting area disturb The grain too much and I only got a few tiny fruits popping up in what I assume was the couple areas that were least disturbed by the jostling of the bag being moved. Their growth requirements are completely unique from any other mushroom cult


--------------------
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #27262883 -

Well there goes that idea.....

Make since man. And I'm sure as fuck not going to try and backtrack when you clearly figured out why people aren't easiing their production.

So where I'm at then, of I want to grow in anything bigger than jars I'm going to need UV sterilization.... Or maybe I can find some thick appropriate plastic containers I can fit into my 55gallon steamer?.. have you had any ideas on expanding past jars?

What's an average yield on a good jar, dry?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
 DMT for IandI

Extras: Filter Print Post Top
Re: Cordyceps Militaris [Re: QM33]
    #27262889 -

Yeah you can do larger totes that’s what I’m doing next


--------------------
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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Re: Cordyceps Militaris [Re: Jakeoncid419]
    #27262893 -

how are you sterilizing them?
Could I put the substrate in the tote then in my steamer?
Rather than sterilizing the substrate in a bag then mixing with LC in the container. Maybe just put the sub in the container, sterilize, then mix the LC in, in the container..?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
 DMT for IandI

Extras: Filter Print Post Top
Re: Cordyceps Militaris [Re: QM33]
    #27262895 -

I actually had better luck with bags....that and it's alot easier to run a few bags through the pc than do a bunch of instapot runs


--------------------
Where am I at?


[/url]
“To be godless is probably the first step to innocence," he said, "to lose the sense of sin and subordination, the false grief for things supposed to be lost."
So by innocence you mean not an absence of experience, but an absence of illusions."
An absence of need for illusions," he said. "A love of and respect for what is right before your eyes.”― Anne Rice, The Vampire Lestat

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Re: Cordyceps Militaris [Re: redeyesmj]
    #27263112 -

You colonized and fruited in a filter patch bag also?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
 DMT for IandI

Extras: Filter Print Post Top
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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Mushroom-Hut Grow Bags

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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