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InvisibleR.I.P.Zappa
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25988389 - 05/12/19 04:25 PM (4 years, 10 months ago)

:ohyeah:


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-The heaviest thing one will ever carry is a thought-
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Offlinethecynicalstoic
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: R.I.P.Zappa]
    #25988396 - 05/12/19 04:36 PM (4 years, 10 months ago)

Okay. This is cool. Totally following along.

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OfflineHugh Jorgan
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25988442 - 05/12/19 05:02 PM (4 years, 10 months ago)

Nice to see ya myco. Looks like a thread to definitely follow!!


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OfflineMycologist217
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Hugh Jorgan] * 1
    #25988827 - 05/12/19 09:18 PM (4 years, 10 months ago)

DAY 0iii-

Alright so a fair amount to talk about today as it was this grow's first encounter with sterile work, but before we jump into that we will talk about what we did first today.

So while we should have started the day with the sterile work, we had to spend all afternoon at the girl's mom's house. By the time we got home the silicon applied to our new LC lids had fully cured so I started with completing the Liquid Culture Lids that should end up being the Flagship of this entire grow. Keep in mind our LC will be used to inoculate 60 grain jars. That means a syringe needle will be piercing our SHIP a couple to several dozen times. I am very confident in the performance of the high temp silicon and have used it for grows of this caliber before. So finishing off the procedure for the LC lid...pictorial:


So after we got a couple sturdy lids constructed for our LC's, we got an area of our house ready to set up our SAB to inoculate the 8agar plates we prepared yesterday.

MATERIALS
-Still Air Box (SAB)
-Scalpels
-Alcohol Flame Lamp
-70% Isopropyl Alcohol
-Cleaning Solution w/ Bleach
-Exam Gloves
-Facemask
-Long Sleeve Shirt
-Duct Tape

We got a small nook cleaned up and wiped down with our cleaaner with bleach product. Before setting our SAB in place in our nook. We placed the alcohol lamp outside of it along with the isopropyl alcohol. We brought our cool pressure cooker into the area and opened it and placed from it our 8 new blank plates and our foil wrapped scalpel into our SAB. We then sprayed the SAB down with cleaner w/bleach again.

We left the area and scrubbed our face clean and  brushed our teeth and used mouthwash. We donned a clean long sleeve t-shirt and put in a pair of nitrile exam gloves. We used duct tape to secure our sleeves to the gloves so as none of the skin on our arms was exposed. a facemask was put on and After spraying down our arms with cleaner w/bleach we entered the area with the SAB.


Following techniques outlined in the Agar work videos link in my signature i proceeded to make 8 transfers from one of the featured M217-b plates (1 to each of our 8 new plates)

I feel fairly good about the procedure and expect good results:


After the new blank plates were inoculated, we put them in a nice clean closet that stays around 75F and then we cleaned and got soaking enough WBS for a few quarts of grain spawn to inoculate along with our LC's!:


Tomorrow will really be kicking things off as we will be preparing and sterilizing a couple blank LC's with the hopes they come out clean and vivacious. If they do they will carry us throughout this whole grow.

The two grain jars inoculated along side the LC's will also be prepared and sterilized tomorrow which puts us at a Tuesday inoculation of our LCs and two preliminary grain jars. That will Mark the official launch of the grow ship and be the official Day 1 leaving us with tomorrow as Day 0iv.

Our two grain jars tomorrow have a couple of purposes/possible destinations. First foremost clean colinzation of grains inoculated alongside the Liquid cultures will be a good indicator our LC is also clean given it was prepared correctly (my forte). Should our grains colinize but our LCs not the grains will be used as masters to go ahead and start a G2G (grain to grain) grow process. If the LC's and grains both colonize cleanly (expected) the two grain jars will be used in the mini "shoebox" style tub we purchased yesterday for a small grow for a soeedier route to pin porn for this log! And a good way to break up the longer road ahead aiming at a 12 tub grow.

Cheers yall! See ya tomorrow!


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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OfflineCorundum
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25988863 - 05/12/19 09:48 PM (4 years, 10 months ago)

:takingnotes:


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:samus::samus:

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OfflinePuduwoke
mushroom enthusiastic

Registered: 06/25/17
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Corundum]
    #25988874 - 05/12/19 09:54 PM (4 years, 10 months ago)

:threadmonitor:


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:sporedrop:Trade List:sporedrop:

LAGM2021

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OfflineMycologist217
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Puduwoke]
    #25990971 - 05/13/19 09:17 PM (4 years, 10 months ago)

-DAY 0iv-

Alright so here we are on the eve of what will be Day 1. I like to start the counter there officially because it is when our primary inoculants for the coming grow will be seeded. It gives a real scale of time for how long it actually takes to go from a single wedge on a healthy agar plate to what will be 12 Monotubs worth of fruit.

So before we talk about what we did during today's prepratory session let's recap what we've done so far:

In day 0 we conceptualized the grow and made a roadmap for it. It included announcing the project and starting this log

In day 0ii we purchased supplies to go ahead and get the grow started. After the supplies was organized at home we began construction of LC lids and constructed lids for agar plates and prepared and sterilized 8 fresh agar plates

In Day 0iii we inoculated our 8 fresh agar plates with wedges from one of two featured M217-b plates. Afterward we finished construction of our liquid culture lids and we got enough grain soaking for 3 preliminary grain jars which brings us into

Day 0iv. Today we prepared and sterilized two 300ml batches of LC medium. The recipe we followed is 285ml water and 15grams 100% honey loaded into jars and steam sterilized at 15psi for 12minutes:


As our LCs ran their short PC cycle we drained and strained our grain from last night. Got it tissue test passable and loaded it into three of our jars. We covered the mouths with pieces cut from our painters coveralls then screwed on our grain spawn lids from Day 0ii:


Grain jars sterilized for 90 minutes at 15psi.

It should be noted all LC and grain jars had their mouths wrapped in foil before going into the pressure cooker.

So after tomorrows SAB sterile work procedure inoculating our LC's and preliminary grain jars, we will have 5-7 days down time while everything starts colonizing. We will fill the gap with progress pics of our agar plates from last night as well as the grains from tomorrow's inoculation!

See ya then!


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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InvisiblePsicomb
shroom or die
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Registered: 01/13/18
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25990999 - 05/13/19 09:34 PM (4 years, 10 months ago)

So pumped to watch this :awetongue:


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
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Offlinelovepotion
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Psicomb]
    #25991026 - 05/13/19 09:43 PM (4 years, 10 months ago)

:thumbup:


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Staying Out to Lunch

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OfflinePuduwoke
mushroom enthusiastic

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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: lovepotion]
    #25991045 - 05/13/19 09:51 PM (4 years, 10 months ago)

Keep it up!
Looking forward to this :takingnotes:


--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021

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OfflineMycologist217
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Puduwoke] * 1
    #25992829 - 05/14/19 05:35 PM (4 years, 10 months ago)

DAY 1

I'd like to start today's entry by saying FUCK Alexandria Ocasio-Cortez...she's hot n all but she's a cunt.

Anyway Day 1 ticked on here today as we mangled our second of 2 featured M217-b plates. By mangled of course I mean that we orchestrated a breezy session in the SAB today inoculating the two prepared LC's from last night as well as all three prepared grain jars. Admittedly less than a third of an agar plate per quart is a little low on the vector scale but I believe we will manage fine.

I've been doing my sterile work in front of my laminar flowhood for so long now being back in a SAB for a featured home grow is a little challenging...very nostalgic.

So here are a few pics of today's bounty:



And now for the next week or so it is the Waiting Game. Good news: the 8 agar plates we inoculated are all about to take off. They definitely alive and kicking.

Alright.

Will post pics of the agar plates when they feeling more photogenic...and once our grain jars start making moves we will keep you updated on those too...other than that...we wait for our Flagship LC's to colonize and mentally prepare to inoculate 60 jars of grains ...oh joy.


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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InvisibleJust_A_Noob
Breathing
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25992868 - 05/14/19 05:58 PM (4 years, 10 months ago)

:headbang3:


--------------------

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Complying = Consent
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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217] * 1
    #25992884 - 05/14/19 06:09 PM (4 years, 10 months ago)

Quote:

Mycologist217 said:
DAY 1

I'd like to start today's entry by saying FUCK Alexandria Ocasio-Cortez...she's hot n all but she's a cunt.






takes one to know one... carry on.

:classictroll:


--------------------
 
the j stands for jesus.
2020 new years grow along

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OfflineMycologist217
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: J. Jack Flash]
    #25992907 - 05/14/19 06:19 PM (4 years, 10 months ago)

Whoevers guess between now and the time the first grain jar gets inoculated with LC is closest to the actual date of the appearance of the first pin produced by this grow will receive one of the 8 plates from Day 0iii. These plates do feature M217-b.

Unfortunately I have retired M217-a and the actual genetics have been exhausted. However whomever can predict the dry weight of the first flush of the first tub of this grow  to the closest gram will receive Spore swabs from M217-a mushrooms.


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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InvisibleBoogieman47
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25992970 - 05/14/19 06:42 PM (4 years, 10 months ago)

I'd say you will see pins 16 days into fruiting ..


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Offlinelovepotion
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
    #25993657 - 05/15/19 01:26 AM (4 years, 10 months ago)

Quote:

However whomever can predict the dry weight of the first flush of the first tub of this grow to the closest gram will receive Spore swabs from M217-a mushrooms.





!!! 164.6 grams dry !!!  (Mostly just wanted an excuse to quote someone saying “however whomever”)

Edited by lovepotion (05/15/19 05:34 AM)

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InvisibleNothingsChanged
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: lovepotion]
    #25993701 - 05/15/19 02:12 AM (4 years, 10 months ago)

:popcorn:


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InvisibleSunnyDayze
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: NothingsChanged]
    #25993867 - 05/15/19 06:32 AM (4 years, 10 months ago)

:klanwatch:

Really wanted to participate but have to settle for just following along!

Fun idea!


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Offlinenewtomyc
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: SunnyDayze]
    #25994043 - 05/15/19 09:27 AM (4 years, 10 months ago)

I guessing, 14 days until you start seeing pinning from inoculation.


Edit: June 8th for pins.
I gave you 4 days for LC to grow and a couple days for you to check it on agar. Then innoc, 4days to colonize jars,spawn, and 10 days to pin. Then threw a few days on just in case my timing is wrong.

Hope I'm close. I have never done LC so just going by what I have read.

Edited by newtomyc (05/15/19 12:26 PM)

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OfflineMycologist217
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: newtomyc]
    #25994045 - 05/15/19 09:28 AM (4 years, 10 months ago)

Guess dates y'all


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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