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Just_A_Noob



Registered: 12/30/16
Posts: 6,809
Loc: PNW
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26003067 - 05/20/19 09:59 AM (5 years, 7 months ago) |
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Damn I lost
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ibuprofemin
Stranger

Registered: 04/03/19
Posts: 151
Last seen: 4 years, 4 months
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: UncleHoyphae]
#26003112 - 05/20/19 10:24 AM (5 years, 7 months ago) |
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June 20 and 249 g
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: ibuprofemin]
#26005783 - 05/21/19 03:42 PM (5 years, 7 months ago) |
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DAY 8-
So it was 1 week ago today that we inoculated our first LC's and the three preliminary grain jars.
After a couple days recovery the grain jars were given a thorough shaking and allowed to recover and work over the last 4-5 days. Over the weekend, the two Liquid cultures hadn't really taken off yet or shown much activity, so we went ahead and prepared a 3rd one using not 1 but 2 wedges from the prettiest plate prepared on day 0iii.
Since Sunday...things have really booked to life in both of the first two LC's with the one in the squat pint jar looking particularly active. Part of me just wants to wait for the third one because of how active and pretty the mycelium was on the plate. The first two LC's we're prepared with a plate that had been in cold storage for several months. It will be a choice I'll be making on Friday or Saturday when the preceding LC's will be too ready to test/use to ignore anymore.

When we start soaking and sterilizing grain this weekend we will be rolling with whole oats. I've never featured whole oats in an active log on the Shroomery, but I use them pretty exclusively for my gourmets. They are way gentler on the labor than WBS and for 60 jars, oats is what I'm going with.
Went ahead and gave the three preliminary grain jars another shake today. Hopefully they won't need another. I'd estimate them each about 25-30% colonized. Not terrible after a week given that each was inoculated using only about 25-30% of one small agar plate. The grains look slightly dry, but I'm not too worried about it, the mycelium all looked nice. Hopefully they be done in another 5-7days.:

To be honest, I've been kind of not growing actives for a while and with the gourmets it's so much easier using the flow hood at the farm. For my home active grows I used the same Still Air Box from way back when I started growing in 2012 until I stopped growing actives last year. It had the arm holes cut on the short sides (one on each side) instead of both being in the broader front face of the box. For the one I built in my new house, I put the holes on the front and I miss my old SAB...still getting used to this style of it...hope the inoculations go well...I'm sure they will provided the LC is clean...and at least one of them will be.
We will see you again when we start soaking some oats!
Keep the good vibes coming. Have a good week.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217] 1
#26008077 - 05/22/19 07:47 PM (5 years, 7 months ago) |
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DAY 9
I thought I wouldn't be back with you guys til the weekend but the LC in the squat pint is simply too active to ignore anymore.
I got some bird seed soaking right bow (5qts) worth to sterilize tomorrow and noc up on Friday. We will know by Sunday if our LC is clean! I'm planning on doing about 3.5mls per jar so I should have 5-7 day colonization times for each quart.
The grain jars all 3 recovered beautifully from yesterday's shake and they are on their way to full colonization! We should be spawning the preliminary mini tub by Tuesdayish.
Don't forget to get your guesses in for the plate and spores give aways! Guesses gotta be in before I inoculate the first jars with LC on Friday! Whoever guesses to the closest day the emergence of the first pin of this grow wins an M217-b plate, and whoever guesses the weight to the closest dry gram of the first full size mono to flush wins M217-a spores.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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NothingsChanged
Striving for Excellence


Registered: 05/28/11
Posts: 10,676
Loc: North/Western WA
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26008103 - 05/22/19 08:05 PM (5 years, 7 months ago) |
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--------------------

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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26008336 - 05/22/19 10:57 PM (5 years, 7 months ago) |
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217, we’re going to need see some day 10 pics of the grains & the LC’s.
-------------------- LAGM 2.022
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: ttching8475]
#26008728 - 05/23/19 06:15 AM (5 years, 7 months ago) |
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DAY 10-
Quote:
ttching8475 said: 217, we’re going to need see some day 10 pics of the grains & the LC’s.

Just for you:

The myc in the grain jars still looking very healthy, clean, and unimpeded!
The myc density in the LC is getting greater all the time!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26008886 - 05/23/19 08:07 AM (5 years, 7 months ago) |
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-------------------- LAGM 2.022
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Zakkery



Registered: 10/15/18
Posts: 299
Loc: UK
Last seen: 4 months, 24 days
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: ttching8475] 1
#26009347 - 05/23/19 01:01 PM (5 years, 7 months ago) |
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Watching and learning. As well as making my move from lurker to poster.
Also June 15th, 212g.
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SamAtticus


Registered: 02/27/18
Posts: 175
Last seen: 1 year, 5 months
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26009509 - 05/23/19 02:20 PM (5 years, 7 months ago) |
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Following
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"I learn by going where I have to go." Theodore Roethke
Bod's AF Search Engine Tek
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,089
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: SamAtticus]
#26009513 - 05/23/19 02:22 PM (5 years, 7 months ago) |
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sh4d0ws is in the house

Looking forward to your fruits
Really thinking I should have cloned this MS PE I just ran that did super well...but I took none!
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: sh4d0ws]
#26009628 - 05/23/19 03:20 PM (5 years, 7 months ago) |
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Day 10 cont-
So we went ahead and got seven quarts of grain sterilizing right now to be inoculated in the morning! I wrapped an empty syringe in foil and put it in with the grain to sterilize.

We also made the decision to make some grain jar lids out of the metal lids that came with the jars. We drilled two 1/4" holes in 14 metal lids and went ahead and applied high temp silicon to one of the holes on each lid to create a ship. This will be easier than the poly filled stuff injection ports I usually use. The other hole on each lid will be crafted into a gas exchange filter. I'll post pictures tomorrow after the silicon sets and the filters are completed.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26011426 - 05/24/19 05:04 PM (5 years, 7 months ago) |
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Day 11-
It is now too late to submit guesses to enter into the give away. Good Luck to all those who submitted guesses!
With that said, the first seven grain jars have been inoculated with LC. Each of the seven jars was inoculated with 2.5-3.5mls of LC. I filled the syringe twice for the procedure.
On one of the inoculations, needle cooled so rapidly that it pulled the polyfill filter out of the lid. I replaced it promptly after the procedure.
I foresaw the issues with the polyfill filter and the hot needle, so yesterday I started construction of grain jar lids with SHIPs, and simple GE filters.

Working on cranking these out in Mass over the next few days. Went ahead and got 14 ready to keep soaking, sterilizing,and inoculating grain.
Will post pictures of inoculated grain as they start colonizing...clean or dirty.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26014342 - 05/26/19 01:05 PM (5 years, 7 months ago) |
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Day 13-
So Late last night I checked the jars inoculated Friday afternoon with our LC and all seven are showing distinct mycelial growth! This indicates a clean LC as any present contamination would show first thing first. We move forward!
What to expect about 36ish hours after inoculation with clean LC:

Very good! We went ahead and sterilized the next batch of grain last night and will be inoculating it this afternoon.
Our 3 preliminary grain jars are very quickly approaching full colonization! And should be ready to spawn by Wednesdayish:

We are still cranking out our SHIPped up grain jar lids:

And here's a photo of the tall LC which remains untested:

Keep the good Vibes coming. Happy Memorial Day
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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NothingsChanged
Striving for Excellence


Registered: 05/28/11
Posts: 10,676
Loc: North/Western WA
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26014446 - 05/26/19 02:10 PM (5 years, 7 months ago) |
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26014498 - 05/26/19 02:47 PM (5 years, 7 months ago) |
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Are you innoculating with the tall, untested LC pictured here?
-------------------- LAGM 2.022
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: ttching8475]
#26014527 - 05/26/19 03:07 PM (5 years, 7 months ago) |
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Not today. Today i'm going to use the squat one I already tested. I'll use the tall one tomorrow and we will know by Tuesday or Wednesday if it is clean.
And we still have the third LC catching up too
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26014540 - 05/26/19 03:13 PM (5 years, 7 months ago) |
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Got it. I had been thinking you’d already used the squat LC up. Did you aspirate the entire LC into syringes during the previous inoculation session or just enough for the test jars?
-------------------- LAGM 2.022
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: ttching8475]
#26014544 - 05/26/19 03:15 PM (5 years, 7 months ago) |
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I just fill up a single syringe during inoculations. Using about 3mls of LC per jar.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 9 months, 26 days
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Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#26014634 - 05/26/19 04:17 PM (5 years, 7 months ago) |
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A cultivation thread with learning objectives??? Love it!
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