|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS 5
#25984964 - 05/10/19 09:23 PM (5 years, 8 months ago) |
|
|
Hey Again Shroomerites! It's your old pal Mycologist217 here, and you guessed it! It's time for our annual summer grow log again! I'd like to welcome back any readers from my previous summer logs as well as new readers alike! I invite you to turn up your AC as we get hot in heavy in this summer's log! This season we are proud to present to you the debut log featuring my very own M217-b Penis Envy Isolate! It is a second generation clone originating from our very well loved friend:
The M217-a clone:

From our latest M217-a grow we went ahead and made the move to try.out a second generation clone. Unsurprisingly, a couple of beautiful isolate plates were close to easy to scale to:

Which brings us to the announcement of this grow log! If you are unfamiliar with my work and contribution here on the Shroomery, I encourage you to check out my best works Here! So!! With open hearts and an empty stomach let's jump right into our:
EXPERIMENT: 2ND Generation Clone Grow: PE M217-b; Agar>LC>Grains>CVG Monotub
LEARNING OBJECTIVES: -Demonstrate prolific knowledge of home mushroom cultivation as it applies to the Psilocybe Cubensis. -Demonstrate profound practical skill in home mushroom cultivation as it applies to the Psilocybe Cubensis. -Demonstrate advance practical knowledge of such concepts as: no pour Agar work, strain isolation, tissue cloning, liquid cultures, grain spawn, and of course the Monotub -Successfully use a 2nd Generation Clone to propagate a vivaciously successful magic mushroom grow. -Maintain an extremely high rate of contamination free mycology. -Utilize homemade original designs of vessels, lids, and/or tools throughout a successful grow. -Observe how identical genetics differ in phenotype across a dozen individual Monotubs.
MATERIALS We will be using a myriad of equipment this go around. If you need help looking into more specific materials I encourage you to inspect my INVENTORY OF REQUIRED MYCOLOGY SUPPLIES. But! Past that generalized list we will need: -Genetics (M217-b on agar) -Pressure Cooker -Scalpels -Syringes -Liquid Culture Vessel -Liquid Culture supplies -Preferred Grain spawn -Preferred Spawn Vessels (in this case quart sized ball jars w/ modified lids -Monotub Supplies -Substrate Components (in this case coir, vermiculite, and gypsum -Casing Materials (Jiffy Mix) -Positive Vibes and a Good attitude.
PROCEDURE/METHODOLOGY Alright you guys! So for the remainder of the time this thread is active it will be updated with the procedure in real time in the form of edits to this OP as well as entries in the thread below! Check back often throughout the next 45ish days for regular updates as we make magic!
We will be starting in the next couple days by preparing a new batch of blank agar plates to expand one of our featured plates on to. We will also prepare a preliminary liquid cultures as well as a couple grain jars to inoculate directly with our second featured plates! We will be using my very own Liquid Culture Tek. And for our grains we will be using Whole Oats for some and WBS for others!
Strap in everyone... I encourage you to subscribe, contribute, and enjoy another Mycologist217 Real Time Grow Log
EDIT: WORK IN PROGRESS
So we started working on this project round about the 10th of May . The project was started by compiling the experiment idea, learning objectives, materials list, and announcing the project via the starting of this grow manual!
By May 11 we had acquired all the necessary materials to begin our project:
  Our project began with the expansion of our existing culture stock onto 8 new plates. We used materials such as 4oz glass jars, medical tape, painters coveralls, agar powder, instant mashed potatoes, scalpel, a pressure cooker, and other agar supplies detailed more thoroughly in the log below:

We took that opportunity to refresh ourselves in proper sterile technique and had our lab stocked with nitrile gloves and facemasks, 70% isopropyl, an alcohol lamp, and bleach cleaning solution with paper towels. A Still Air Box was also constructed for use during all sterile work for the project.
After the expansion run on to 8 new plates, the second initial culture plate was used to inoculate 2 Liquid cultures and 3 grain jars. The Liquid Cultures were prepared using a solution of water and honey at a ratio of 285g water/15g honey. And was housed and sterilized in custom built LC vessel lids with protected GE filters and Self Healing Injection Ports:
   
We then entered a kind of quiet before the storm phase while we waited for our LC's to come to life and our agar inoculated grains to colonize. In the interim more.supplies was acquired for the Fuller grow spectrum including coir, vermiculite, gypsum, and we started construction of more sophisticated style of grain lids featuring SHIPs for easy inoculation via LC.
We also allowed our newly prepared round of 8 Culture plates colonize before moving them into cold storage. One very pretty candidate was selected from the lot and used to prepare a third Liquid Culture to be used in the latter half of this grow.:

Around 10 days after inoculation, our premier Liquid Cultures were deemed ready to start using, and we started the process of soaking and sterilizing and inoculating 5-7qts of grain a day until we had 33 LC inoculated grain jars:

We made the decision to wait until these jars are all fully colonized and spawned before doing more grain, which allows our third prepared LC time to colonize and boom to life.
On June 2nd, around 19days after inoculating our first two LC's and the three preliminary grain jars, it was time to spawn a minimono tub with them as a teaser of things to come! We spawned in a 28ish qt tub using half a brick of coir, 1.25qts vermiculite, a handful of gypsum, and 2.75-3ish qts water.
We did not fruit at spawning and just let the mycelium work for 5 days before casing and fruiting:
    
More soon
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (06/07/19 01:05 PM)
|
Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,405
Loc: Over by your Mama's house
Last seen: 4 hours, 9 minutes
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25985039 - 05/10/19 10:13 PM (5 years, 8 months ago) |
|
|
I'm on board for this ride 😁
|
Mr. Funguy
Quasi Frodo


Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 5 years, 2 months
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Crackatoa]
#25985044 - 05/10/19 10:19 PM (5 years, 8 months ago) |
|
|
This looks interesting...
--------------------
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,382
Loc: eating the cats
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Crackatoa]
#25985046 - 05/10/19 10:19 PM (5 years, 8 months ago) |
|
|
|
Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,487
Last seen: 7 hours, 38 minutes
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: mushboy]
#25985066 - 05/10/19 10:40 PM (5 years, 8 months ago) |
|
|
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: mushboy]
#25985081 - 05/10/19 10:56 PM (5 years, 8 months ago) |
|
|
Day 0-
So just a few more opening day footnotes:
The first thing is that this is a fairly advanced grow style I will be employing as far growing cubes go, I have a decent grasp on it and will usually talk from a perspective expecting the reader to also. If you are a newer cultivator on board for this grow feel free to ask any questions you might have about the techniques demonstrated and I will explain the why's and how's of it.
Thing number 2 of it is that many many Monotubs will be grown in this grow. At least 10.
The number 3 of it is I moved into a larger home in March than I had ever previously lived in as an adult person. It has a two car garage featuring a very nice utility closet with large space and U shaped shelves. Might be a good place to set up a SAB, if not I think I'll go with the laundry room for the SAB. I used to use my laundry room i my tiny apartment when I started out years and years ago. At my last house I used the Harry Potter Closet under the stairs. I almost blew myself up with my alcohol lamp a couple times but I'm better at making them now...which leads us to the subject of this footnote: alcohol lamps and sterile technique.
I am a huge fan of the homemade alcohol lamp. Back in the day I fueled them with 91% Isopropyl and never really had any problems but a few years back I switched to denatured alcohol. I really enjoy it. It burns hot, getting needles red hot in a second or two and scalpels in a couple of seconds.
I have done experimenting with which jars to use for home made alcohol lamps and the vessel I've had the most success with is done with a small minced garlic jar:  
Very simple design...make hole in the lid of the jar with pliers and pull a stretched out cotton ball through the hole...half way. Fill the jar with fuel and allow cotton wick to soak in the fuel for a while before lighting. Cool little trinket to build to start a grow off.
Alright see you when we are ready to make our blank plates where we talk about that methodology in great detail.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25985101 - 05/10/19 11:19 PM (5 years, 8 months ago) |
|
|
Myco what's up man glad to see you around
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Boogieman47]
#25985111 - 05/10/19 11:28 PM (5 years, 8 months ago) |
|
|
Just living the dream. Really good to hear from you! I love.these forums
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25985151 - 05/10/19 11:53 PM (5 years, 8 months ago) |
|
|
Yeah same here my friend cant wait to see your grows you always kill em woth that culture
|
shroomify

Registered: 04/14/19
Posts: 51
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25985347 - 05/11/19 04:31 AM (5 years, 8 months ago) |
|
|
Awesome, looking forward to this!  How do you store your alcohol lamp when you're finished? Does it evaporate after some time?
|
dr psilocybin
Stranger

Registered: 01/22/19
Posts: 27
Last seen: 4 years, 10 months
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: shroomify]
#25985378 - 05/11/19 05:23 AM (5 years, 8 months ago) |
|
|
following
-------------------- http://
[
|
newtomyc
enthusiast


Registered: 12/24/18
Posts: 1,038
Loc: here there and everywhere
Last seen: 1 year, 8 months
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: shroomify]
#25985380 - 05/11/19 05:24 AM (5 years, 8 months ago) |
|
|
Following
-------------------- JJ
Draw unto others as they have been drawn to you.... WSP
|
J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: newtomyc]
#25985408 - 05/11/19 05:53 AM (5 years, 8 months ago) |
|
|
*pulls up stool, pad and pencil*
--------------------
the j stands for jesus.2020 new years grow along
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: shroomify]
#25985584 - 05/11/19 08:38 AM (5 years, 8 months ago) |
|
|
I usually just it out. Soared fuel evaporate very quickly. I try my best by now to be very conservative with how much fuel I fill it with since a lot goes to waste.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Asura
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
Last seen: 3 hours, 41 minutes
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25985846 - 05/11/19 11:14 AM (5 years, 8 months ago) |
|
|
|
Failboat
Free man

Registered: 02/01/18
Posts: 8,736
Last seen: 1 month, 1 day
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Asura]
#25985898 - 05/11/19 11:41 AM (5 years, 8 months ago) |
|
|
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Failboat]
#25986838 - 05/11/19 08:54 PM (5 years, 8 months ago) |
|
|
DAY 0ii-
So today was the chore day to get the ball rolling. I trashed a bunch of my equipment when I moved into this new house so I had to go buy a lot of supplies to make sure I'm good to begin. Total Costs today: 224$. I still have to spend another 100$ or more on more jars, grain spawn, coir, verm, gypsum, and tubs...12 tubs has some serious overhead cost. But I purchased a smaller tote to inoculate with just 1 quart of spawn to hopefully cover the costs before the 12 tubs fruit.
So what did I buy today?:

Yeah...ton of stuff but we got everything we need to start making some new plates and get a few grain jars and LC's going which speaking of LC we constructed a few of our custom LC jar lids:

After I got the silicon setting in the three LC lids I made 8 agar lids and a few preliminary grain jar lids:
     
.
After that I started getting everything organized for my sterile Tek lab with the SAB. I went ahead and constructed a new alcohol lamp and prepared a jar of isopropyl soaked cotton balls. Got paper towels set up and my gloves, facemasks, and disinfectant.
After all that I went ahead and prepared 8 agar plates for sterilization and threw them in the pressure Cooker:
 For this agar recipe we combined 125ml water with 2.5g agar powder, 2.5g potato flake, 1.75g dextrose, and .15g nutritional yeast. I also colored it with 1 drop blue and 1 drop red food coloring. These will be inoculated tomorrow when they are cool from one of the two featured M217-b plates for the purpose of expanding the culture and inoculant stock.
Tomorrow when the silicon in the LC lids are set I'll finish them off and pressure cook 3 different jars of liquid culture. We will talk in much greater detail then about LC recipe and preparation
Edited by Mycologist217 (05/11/19 09:30 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,382
Loc: eating the cats
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217]
#25986845 - 05/11/19 08:57 PM (5 years, 8 months ago) |
|
|
why not upgrade to a torch for flaming tools? weeeelllll worth it.
and you can take dabs while you work
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: Mycologist217] 1
#25987028 - 05/11/19 10:52 PM (5 years, 8 months ago) |
|
|
Nice tag.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 29 days, 15 hours
|
Re: ~INTERACTIVE SUMMER GROW 2019~ PRESENTING THE M217-B PE CLONE~ LAB MANUAL STYLE WITH PICS [Re: bodhisatta] 1
#25988116 - 05/12/19 01:34 PM (5 years, 8 months ago) |
|
|
What?! Holy Smokes!! Thanks you guys!!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
|