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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help
#25960439 - 04/28/19 10:36 AM (5 years, 8 months ago) |
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Good morning everyone hope everyone is having a great day today I've had a problem the past couple of months and I will not give up on my hobby. But this sucks I have like 30 jars of blue meanie white mycelium looks and smells beautiful but I have no growth when I transfer to monotubs. I did notice the liquid culture that I was using was about 8 months old would this cause this problem? I was also having this problem in the humid months in Northern California have not had problems in spring summer and fall I'm wondering if the humidity might have helped any kind of input would be greatly appreciated. Have a great day thank you
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70] 1
#25960443 - 04/28/19 10:40 AM (5 years, 8 months ago) |
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pix? I kant reed.
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: mushboy]
#25960458 - 04/28/19 10:57 AM (5 years, 8 months ago) |
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Post pics if you want help.
-------------------- Learn about breeding
 
C10βs agar guide
Good surface conditions = Good pinsets
Read more, post less.
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π° πΌ π΄ π
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π΄ π° πΌ π² π» πΈ π½ πΆ π
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: AyePlus]
#25960497 - 04/28/19 11:29 AM (5 years, 8 months ago) |
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  thanks for your help I will post more pictures
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25960506 - 04/28/19 11:35 AM (5 years, 8 months ago) |
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 okay guys there's a couple more pics thank you for your help
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Psilanthropist
Noob

Registered: 06/23/16
Posts: 66
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25960516 - 04/28/19 11:40 AM (5 years, 8 months ago) |
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They look bacterial
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Psilanthropist]
#25960529 - 04/28/19 11:50 AM (5 years, 8 months ago) |
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Just out of curiosity what are you seeing that makes you say that they were all shaken and have come back as well.
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Eazybreezy02


Registered: 02/07/19
Posts: 23
Last seen: 1 year, 11 months
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Psilanthropist]
#25960536 - 04/28/19 11:54 AM (5 years, 8 months ago) |
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What tub tek are you using? What is the setup, conditions, etc? How long have the tubs been inoculated? Post pics of the tubs too please.
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Apc123
Mushroom momma



Registered: 04/26/18
Posts: 1,243
Loc: United States
Last seen: 18 days, 23 hours
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Eazybreezy02]
#25960543 - 04/28/19 12:02 PM (5 years, 8 months ago) |
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LC are best at there peek. Normally about a month from inoculation.
Those jars are definitely bacterial. How long ago did u spawn to the mono? Maybe u just need to give it some more time...
-------------------- The thing about deceit is, you don't know your being deceived...
The only thing I am sure about is that I know nothing - socrates
Believe nothing you hear and half of what u see.
  
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Psilanthropist
Noob

Registered: 06/23/16
Posts: 66
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Apc123]
#25960615 - 04/28/19 12:42 PM (5 years, 8 months ago) |
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The wet spots and the areas where the myc is smashed against the glass indicate bacteria. This is some good info from another thread:
Quote:
Mad Season said: This is from a wild print right? Usually wild prints have insane fluctuations with genetics compared to the domestic ones. They're usually the ones to show the most phenotypes, and is the reason I'd love to use a wild print one day.
The fact they're all blobbing says a few things.. Lack of fae, too much nutrition and/or contams would cause this. Since this is a wild print id bet fae iz a major player, also the fact is that the last pic of grains posted is not exactly the best looking ones, and they took pretty long to colonize, with some visible wet spots. I've always used this as a measure for good grains vs slightly bacterial:
Quote:
Mad Season said: Oh dear lord no. I'll get a quote from when I had this debate awhile back. There's a big difference between fully colonized and cleanly colonized.
Quote:
Mad Season said: Well there's a difference between colonized and cleanly colonized.
This is colonized: 
Lots of members have noticed cobweb and trich from jars that look like this or better. Wet spots are bacteria. Not wet grains or wet spots. To colonize cleanly is the hardest. I had to change my pc times to 2 hours to reduce it.
This is (ALMOST) cleanly colonized, that's how hard it is to have it completely clean. This isn't even completely clean:

This is completely clean spawn:

Anyone who's done this for awhile will testify this is the hardest part of the hobby and is what will dictate the success or failure of bulk.
All those jars had a mushroomy smell. A miniscule amount of bacteria will get overpowered by the mushroom musk. Especially if it is covering the surface
Quote:
Mad Season said: They didn't have wet grains stuck to the jar side. It was bacteria. A properly pasteurized bulk substrate has beneficial microbes that fight bacteria off. In fact if mycelium takes the upper hand like the jars in those pics, more often than not it won't be a problem. However it will ruin pinsets, slow down colonization rates, and EXTREMELY increase chances of other contaminations like trich or cobweb.
All those jars were used to make their own separate trays. The ones that were clean easily pumped out twice as much weight.
Dirty spawn:

Clean spawn:

This has been my experience. I've definitely noticed that grains colonized with absolutely no wet spots perform the best. I noticed your jars last posted had wet spots, although very minor, but as you can tell even minor wet spots have ime caused issues. This is what makes spawn the fuckin hardest part of this hobby. Especially in a contam ridden environment. I'm glad you're getting mushrooms coming in. I think you seriously need to up the fae tho.
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Psilanthropist]
#25962514 - 04/29/19 11:53 AM (5 years, 8 months ago) |
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http://  I appreciate your help with that thanks for the info. So here I have another blue meanie one that looks a lot better I don't know if I should do a mini monotub because it looks better and I don't even know where to start to look for where this contamination problems coming from I don't know if it's grain preparation my liquid culture any clues on how to find out? Thanks
Edited by JackStaw70 (04/29/19 11:54 AM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25962645 - 04/29/19 01:04 PM (5 years, 8 months ago) |
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Quote:
JackStaw70 said: So here I have another blue meanie one that looks a lot better I don't know if I should do a mini monotub because it looks better and I don't even know where to start to look for where this contamination problems coming from I don't know if it's grain preparation my liquid culture any clues on how to find out? Thanks
Yeah, they a bit bacterial, but it shouldn't be so bad that they don't produce fruits. Few questions:
- What teks/materials are you using to spawn?
- How long did they go before you say you have "no growth"?
- Where did you get the spores from (don't list a vendor please, was it prints from a friend, spores from a vendor, etc?)
- Are you using agar first?
- Do you have any pictures of your lack of harvest?
It'll be easier to help if we know how you got to where you are. Part of me is thinking you'll just need to wait longer or possibly you're spawning into bad substrate (like, if it's way above field capacity).
With that said, rather than a mini-mono I highly recommend going straight coir to shoeboxes. It is the perfect no muss-no-fuss arrangement. Spawn it at 1:1 or 1:2 (spawn:coir) ratios. Here's some good teks for it:
Bod's Bucket Tek for coir Bod's Stupid Simple Shoeboxes
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70] 1
#25963068 - 04/29/19 05:29 PM (5 years, 8 months ago) |
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Idk those look pretty fuckin bad.. Kinda doubt they'd even recover from a shake. Not surprised the myc is barely growing when spawned. I would seriously recommend you stop using LC > grains, and focus on agar cleanup. Every jar posted is hella bacterial. If you can get clean agar, you at least have clean inoculant and a good idea of what open media sterile tek in a sab is like, then work on spawn prep and whatnot. Work on things in steps, starting with the first step, which is the inoculant.
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Mad Season]
#25963696 - 04/30/19 01:14 AM (5 years, 8 months ago) |
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Thank you guys so to give more detail as per requested. These tubs have sat for more than 5 weeks . I had several and they all contaminated except for one and it never flushed so I soaked it and agitated the top surface like I would do for a second flush and I finally got a first flush the barely over an ounce dried. I use Damions 5050s coir tek and doc wbs tek. I have had great results in the past but I think I narrowed it down from just what you guys stated it's got to be my liquid culture. The liquid culture came from a Spore print that I took myself from one of my first harvest. And all my first grows that had great results came from a Spore syringe bought from a reputable Spore Supply. So it seems I need to go back to square one and get some reputable spores and then I want to try agar because from what I've read liquid culture it's not that reliable and the agar is the only way to truly know if im contaminant-free to inoculate my jars. I really appreciate your time I'm sorry I don't have more pictures. But I do have some pictures of some Costa Rican good I'm going to post tomorrow if that's okay cuz I guess I'm having a hard time identifying the contaminant I see the wet spots at and I looked at some of the old pictures of my first grain spawn and the mycelium from my original grows from the Spore syringe. And yes I see how much healthier they look. I will definitely start taking more pictures with any issues that I have so that I can really resolve this. I stopped posting for a while I must admit I got a couple snotty comments when I was genuinely just trying to ask a real question I know the world isn't perfect but and some people just have bad days or bad attitudes I have to accept that. But I appreciate I really appreciate you guys taking the time and put in the effort out to help me identify this problem thanks again Mad Season and ShaperDreaming
Edited by JackStaw70 (04/30/19 01:37 AM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25964244 - 04/30/19 11:23 AM (5 years, 8 months ago) |
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Sounds like you're on the right track! Best of luck moving forward!
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: ShaperDreaming]
#25964580 - 04/30/19 03:16 PM (5 years, 8 months ago) |
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So this is my latest Costa Rican no grain to grain yet I was just about to grain to grain it but from what it looks like and from the pictures it's contaminated to where do I find references for this I looked up in the search engine. Under signs of contamination in mycelium. Any clues on where I can find this so I'm not relying on you guys I appreciate your help but I need to know this for myself thanks for your help.
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Psicomb
monotub with eyes



Registered: 01/13/18
Posts: 4,917
Loc: WA
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25964586 - 04/30/19 03:21 PM (5 years, 8 months ago) |
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Looks safe enough to me. Not perfect but it should get you shrooms from the jars you transfer to.
https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1 this is a really good link for how to sort out what everything should (and shouldn't) look like.
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand
Edited by Psicomb (04/30/19 06:08 PM)
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SpikeSpiegel
Mold Hand



Registered: 07/30/18
Posts: 162
Loc: EUSSR
Last seen: 5 months, 10 days
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: Psicomb]
#25964855 - 04/30/19 05:53 PM (5 years, 8 months ago) |
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You don't need more pictures. If you're looking at past grows and seeing that the cultures looked healthier than your current jars then you know what to look for. And Mad Season's pics upthread are perfect.
LC is not unreliable, it just takes close to flawless sterile technique to do it reliably. If you had success in the past and you feel like you know what you're doing then I recommend you just add an agar step in between spores and LC. Get a perfect looking plate and pull from that to make your LC. It doesn't take much of anything to innoc LC. Check out Josex's biopsy poke tek. Great way to get clean LC from agar.
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XnMe
Somth'n of a Somth'n



Registered: 06/23/16
Posts: 1,284
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: JackStaw70]
#25964882 - 04/30/19 06:16 PM (5 years, 8 months ago) |
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Quote:
JackStaw70 said: So this is my latest Costa Rican no grain to grain yet I was just about to grain to grain it but from what it looks like and from the pictures it's contaminated to where do I find references for this I looked up in the search engine. Under signs of contamination in mycelium. Any clues on where I can find this so I'm not relying on you guys I appreciate your help but I need to know this for myself thanks for your help.
Looks like more bacteria imo. Clean spawn looks like this..(oats) See how the myc isn't so thick and fluffy, and barely has excess water on the walls of the jar.
 A2G day 14 w/ 2 shakes. Do a smell test on that too, if it smells like feet or it's fruity it's no bueno..
--------------------
Thanks too all
Best Tek List, click it
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southbounpachyderm
Stranger
Registered: 07/20/18
Posts: 282
Last seen: 4 years, 5 months
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: XnMe]
#25964956 - 04/30/19 06:56 PM (5 years, 8 months ago) |
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Personally I would not grain to grain that jar. I g2g'd a similar looking jar and while I got fruits. 3 of the tubs I made out of the g2g'd jars ended up with mold and the other 2 tubs barely gave me 3oz's each and did not last for a second flush.
Fruit what you have in shoeboxes and start working on your agar skills.
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JackStaw70
learning


Registered: 07/31/18
Posts: 59
Loc: Nor Cal.
Last seen: 4 years, 1 month
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Re: Nice white mycelium in wild bird seed jars but once transferred to monotub no growth please help [Re: southbounpachyderm]
#25965492 - 05/01/19 12:56 AM (5 years, 8 months ago) |
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Thanks so much for the info. back to the drawing board. but at least I have a bunch of good grows under my belt to inspire me to get back to that. Someone told me in my beginning grows that I did really well with that now the key was to stay consistent. Truer words were never spoken I can't thank everyone enough peace.
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