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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Trich during / after first flush on almost all my tubs - help me diagnose why?
#25954118 - 04/25/19 06:07 AM (5 years, 8 months ago) |
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After getting a few very decent first flush harvests from my first batch of cube bulk grows, I've had an epidemic of trich hit over the past week, and it's really shaken my confidence. Hoping to get some feedback to help figure out if this is a problem in my technique, my environment, or just bad luck.
Relevant details:
- Between 3/24 and 4/7, I spawned various grains to bucket tek CVG (using Plantonix coir bricks) in ratios between 1:1 and 1:3, with a 1/4" to 1/2" top layer on each. In total I made 6 shoeboxes and one 30 qt mini-mono, all unmodified tubs.
- All grains were sterilized properly (90-120 minutes at 15 PSI) in a PC. Most were from clean agar, though I did have a few inoculated from spores before I got my agar work up and running.
- Other than the spore inoculations (through SHIPs), all work was done in a SAB. I'm still pretty new, but based on my results on about a hundred agar plates (about a 90% clean success rate), I'd say my technique is at least average I think.
- Of the shoeboxes, I had two (one GT and one PF Classic) put out decent first flushes and then show trich before second flush (subs were dunked for a couple hours in between). Another GT box triched out before first flush but after the top layer colonized. Two more (both PE uncut - these were the ones straight from spores) started putting out small, sparse first flush fruits, then stalled before triching out. The sixth shoebox had a decent first flush and is now most of the way into second flush and looks clean.
- The mini mono is almost ready to harvest first flush (first veil of a thick set broke overnight) and fruits look good, but just noticed a spot of trich on the side this morning, so I'm giving it the day outside for fruits to mature a little more and then will harvest tonight.
- The mini mino was my "dump tub" of misfit grains that were left over because they colonized slowly or didn't take after the first spore injection, so I wouldn't be surprised if a bunch of those grains were dirty, but all the shoeboxes were grains that looked and smelled very clean before spawning.
- Everything was grown indoors in my apartment with natural light and room temperature air, mostly still except for an air purifier on low across the room.
Any red flags yet? Here's what I'm thinking could be going on.
- My main theory is that I kept subs too wet. I think I've got field capacity down, but misted whenever the tops looked dry and bottom watered mid-flush when the subs pulled from the sides. After first flush on the ones that got there, the subs still felt pretty heavy, so my suspicion is that it was too much and perhaps should've let them dry out more. It's been really dry in my apartment though, so I've been a bit paranoid about drying out too much.
- I'm also worried, though, that the spores are airborne throughout my grow space now. Going to pause on spawning for a bit til I can give it a deep cleaning and air out more just in case.
- The coir is organic and the manufacturer says it doesn't have trich, but I might try proper pasteurization in the future just in case.
- Other than the GT tub that triched before first flush, all others either made it through a flush or had suspect grains (the mini mono "dump tub" and the PEU spores), so I suppose it could just be bad luck? (If so, would love some reassurance here.)
- Also had a bunch of cakes and top-fruited jars in a sgfc nearby, and got trich in a couple jars there before it really hit the bulk, so maybe it started there and migrated? (The air purifier could be a vector, though it's a household-quality HEPA model so at least shouldn't be making things worse...)
Anyone else have other ideas here? I've got a bunch of jars incubating, but right now I'm feeling like I should pause everything until I figure this out so I'm not just running a giant trich farm. Help? Thanks!
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NPK
Stranger

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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25954317 - 04/25/19 08:53 AM (5 years, 8 months ago) |
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I'm new too so can't offer much. However, I use trich to grow plants, and have it all over the house, with no trich yet in my tub. I've just been trusting the spawn. Pretty sure it all about the spawn. I'm on a decent second flush. Hope you get it fixed man.
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SawCeeJack


Registered: 01/25/19
Posts: 1,036
Last seen: 5 hours, 40 minutes
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: NPK]
#25954322 - 04/25/19 08:59 AM (5 years, 8 months ago) |
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Quote:
NPK said: I'm new too so can't offer much. However, I use trich to grow plants, and have it all over the house, with no trich yet in my tub. I've just been trusting the spawn. Pretty sure it all about the spawn. I'm on a decent second flush. Hope you get it fixed man.
Yeah ironic... trich's great for plants.
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: NPK]
#25954463 - 04/25/19 10:49 AM (5 years, 8 months ago) |
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I'mQuote:
NPK said: I'm new too so can't offer much. However, I use trich to grow plants, and have it all over the house, with no trich yet in my tub. I've just been trusting the spawn. Pretty sure it all about the spawn. I'm on a decent second flush. Hope you get it fixed man.
Thanks. One other idea I'm wondering about is if I'm spawning too early. The surface of the grains always looks fully colonized, but maybe I'm missing uncolonized grains or they're not colonized throughout? Maybe an extra week or two would help?
Most likely, though, I'm probably keeping my subs too wet. I have one newly spawned shoebox from this week, and I'm challenging myself to not mess with it at all until first flush is done, no matter how dry it looks. Will see if that helps.
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Mr. Funguy
Quasi Frodo


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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25954503 - 04/25/19 11:14 AM (5 years, 8 months ago) |
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Jumping the gun...
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Mr. Funguy]
#25954574 - 04/25/19 11:52 AM (5 years, 8 months ago) |
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Do you have pictures of your spawn? If your grains were too wet & were bacterial it could have given trich an invitation to the party.
-------------------- LAGM 2.022
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ttching8475]
#25954649 - 04/25/19 12:44 PM (5 years, 8 months ago) |
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Quote:
ttching8475 said: Do you have pictures of your spawn? If your grains were too wet & were bacterial it could have given trich an invitation to the party.
I don't unfortunately, but that's definitely a possibility. They were all white and smelled fine, but could've been hiding something.
Going forward I'm going to try to simplify things a bit - for example, not using WBS because getting the moisture right in that has been way harder than wheat or rye, and using quarts rather than pints since every extra jar is another potential vector for contams - and seeing if I can use that to find indicators of when things are likely to go bad. One problem I've noticed on here is that anytime there's a picture of a jar, someone will say that it's bacterial, so it's not much help for issues that aren't totally obvious. Got to figure that out for myself I think.
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ShaperDreaming
Weirdo



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25954797 - 04/25/19 02:06 PM (5 years, 8 months ago) |
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So, trich is everywhere, not much you can do to get rid of it, but to know how much there actually is (as seen in your tubs) is important. As for being shaken in your confidence, I'm right there with you. I just moved and went from less than 10% contam rates to over 40% with nothing else changing. I was informed it is mold season, so take that into account too. We can get through this phase! If you want to me ever about this I'm happy to chat and offer support. I'm having kind of a tough time with it too.
Outside of spores to grains (you said you moved over to AGAR, which is good) I saw a couple of possible points to clean things up. You mention SAB, but you're likely still doing some things in that SAB that may not be as clean as you want. I'm currently going over many different SAB videos where people do SAB work and am comparing it to mine. (Like Bod's SAB videos)
Quote:
Durgin said: My main theory is that I kept subs too wet. I think I've got field capacity down, but misted whenever the tops looked dry and bottom watered mid-flush when the subs pulled from the sides. After first flush on the ones that got there, the subs still felt pretty heavy, so my suspicion is that it was too much and perhaps should've let them dry out more. It's been really dry in my apartment though, so I've been a bit paranoid about drying out too much.
WOW This is way too much water (well for the shoeboxes at least). If you hit field capacity just right you shouldn't need to mist the boxes at all. This helped the trich if anything though, it didn't create it. Shoeboxes should be completely ignored (with the occasional exception, like PE needs some misting towards the end I find) for the entire first flush after it's spawned.
I did this really frequently when I first started. It created environments that trich'ed out after 2-3 flushes and created smaller, weirder looking fruits. Eliminating that will create better mushrooms and a more challenging environment for trich.
Quote:
Durgin said: Also had a bunch of cakes and top-fruited jars in a sgfc nearby, and got trich in a couple jars there before it really hit the bulk, so maybe it started there and migrated? (The air purifier could be a vector, though it's a household-quality HEPA model so at least shouldn't be making things worse...)
BINGO! Right here. It didn't start there or migrate, this shows that it was in your spawn to begin with.
If you had cakes that went green it is in your spawn. 9/10 trich comes from dirty spawn. Try doing more transfers from agar until you're sure they're clean. Post more pictures on here to check with others if they look clean. Likely it was sneaking in with your myc on agar, it can be hard af to spot and looks "clean" often.
For me I've gone back to working on making sure my spawn is cleaner right now. It's a pain in the ass because I thought I had this down. I had 4/16 jars mold on me recently and 8/20 plates. Unfortunately trich is one of those that doesn't leave great indicators of contam (like mold and bacteria do), so we have to work even harder to not let it get in.
The air around you is dirty, you can't do much about it. If you get a fully colonized clean plate and put it into a clean grain jar you won't get trich (well, you're very very unlikely) once you spawn. When my technique was down and had less than 10% contam rate I had literally sneezed into boxes that went for 5 flushes w/o contam.
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Mr. Funguy
Quasi Frodo


Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 5 years, 2 months
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25954802 - 04/25/19 02:08 PM (5 years, 8 months ago) |
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Ha! Awesome pic shaper!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Mr. Funguy]
#25954812 - 04/25/19 02:12 PM (5 years, 8 months ago) |
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No pictures = no advice just guesses based on your interpretations of what's happening.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25954817 - 04/25/19 02:15 PM (5 years, 8 months ago) |
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Quote:
Durgin said: Going forward I'm going to try to simplify things a bit - for example, not using WBS because getting the moisture right in that has been way harder than wheat or rye, and using quarts rather than pints since every extra jar is another potential vector for contams - and seeing if I can use that to find indicators of when things are likely to go bad. One problem I've noticed on here is that anytime there's a picture of a jar, someone will say that it's bacterial, so it's not much help for issues that aren't totally obvious. Got to figure that out for myself I think.
Ok, so some of these recommendations won't help you too much (like the jar size is fine at pint, just wait until you're down to less than 10% contamination rate to move up to quarts). But some are great ideas, here's another: Switch to only coir. CVG works, it's not that it doesn't, but every additional thing is a vector for contamination. BOD's tek is great, and check his results pictures to see the difference between CVG and coir only.
Here's the thing about bacteria: it's going to take a long time to not get mildly bacterial grows. Don't fucking worry about it. Seriously, just know that you need to create environments where your mushroom mycelium has the leg up (i.e. don't over water). Keep posting your pictures, just take the bacterial comments with a grain of salt, and spawn them anyways. I've had a couple jars where I was like "these are beyond bacterial as fuck... fuckit" and spawned them anyways and they both went for 5 flushes before the toss w/o going bacterial/contaming out. Do pay attention to the comments, do improve your sterile technique as a result, but don't throw out bacterial jars that aren't changing colors wildly. Anything beyond sweaty white is too much, don't let it go too yellow and you're good.
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ShaperDreaming
Weirdo



Registered: 10/30/18
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: bodhisatta]
#25954820 - 04/25/19 02:18 PM (5 years, 8 months ago) |
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Quote:
bodhisatta said: No pictures = no advice just guesses based on your interpretations of what's happening.
Also this. It would be a lot of work, but if you had images of any of these tubs/boxes from spore to trich it would be helpful (agar picture, jar colonizing pictures, box colonizing pictures, and then the trich all lined up). Otherwise the best advice we can really give is that you need cleaner spawn.
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Palmer1997
do€ob do£od



Registered: 03/16/19
Posts: 124
Loc: United States
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25954824 - 04/25/19 02:20 PM (5 years, 8 months ago) |
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I’m a noob but what I’m learning is do you really have all those little things down? Like not having your hand over stuff etc. idk just thought ide chime in cause it doesn’t sound like your sterilizing media wrong.
Or extra spores floating around the house?
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AyePlus
Stony Danza



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: bodhisatta]
#25954828 - 04/25/19 02:22 PM (5 years, 8 months ago) |
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Just an informed guess but in all likelyhood your spawn was dirty and your sub was over saturated. If your sub was shrinking away from the sides but still heavy you were overhydrating your coir. Try cutting the water back by 1/5th or so and don’t mist before first flush unless your surface is dry with no moisture whatsoever. If the spawn was visibly bacterial you’re better off with underhydrated than over.
No way to be sure without pictures. Post some of your newer jars and we’ll have a better/more accurate critique for you.
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jboredone
Money-The root of all evil....



Registered: 01/19/12
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25954851 - 04/25/19 02:29 PM (5 years, 8 months ago) |
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Not sure where you live but in most places its a good time to "plant" used spawn. Dig a shallow hole in a somewhat shady spot and crumble up your used mycelium and cover loosely with soil. Water occasionally.
-------------------- Peace Pot Micro-Dot God Loves You High or Not!!!
In order to grow old and wise, you must once have been young and dumb!

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nulls


Registered: 01/30/19
Posts: 158
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: jboredone]
#25954938 - 04/25/19 03:08 PM (5 years, 8 months ago) |
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Sorry to hear, dude. Can't offer much advice other than praise the move to agar, but also realize that it can be hard to spot contams even in petris. That aside, I'm realizing that my subs are over saturated as well because I was trying to maintain the surface conditions that I'd seen people praise so many times. Don't have trich or anything, but my fruits have that overly wet sub fuzz at the bottom for one. Also the fruits often feel soggy at the bottom when I pick them. Then I read Bod's or RustyWhitSup's posts and realize that I'm overdoing it. I did the same thing when I was first learning to grow cannabis. Anyway, good luck!
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ShaperDreaming
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: nulls] 2
#25954974 - 04/25/19 03:28 PM (5 years, 8 months ago) |
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nulls, you got shoeboxes? Them surfaces are not going to look like the pictures from the "proper surface conditions" thread. Took me a min to realize those conditions only work for dialed in mono-tubs. If you're going un modded it's a bit different.
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Durgin
Amateur Mad Scientist



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25955168 - 04/25/19 05:07 PM (5 years, 8 months ago) |
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Thanks everyone (even bod for classic bod snark). I tossed the tubs before reading these, but did harvest a fresh pound and a half from that mini mono beforehand so that makes me feel slightly better. I've slowed down for the moment but will take more pics in a few weeks when the next batch is ready to spawn.
And in the meantime, I'll enjoy the one second flush tub that's still going (there's a nice monster coming up and a couple dozen others) and leave my newest shoebox alone for the next week or so and hopefully that will go better.
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Seahag
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25955276 - 04/25/19 06:12 PM (5 years, 8 months ago) |
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In for insight as well. First 3 tubs all contaminated during second flush. Next one I'm leaving alone. No. Watering I promise
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nulls


Registered: 01/30/19
Posts: 158
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25955581 - 04/25/19 08:49 PM (5 years, 8 months ago) |
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Quote:
ShaperDreaming said: nulls, you got shoeboxes? Them surfaces are not going to look like the pictures from the "proper surface conditions" thread. Took me a min to realize those conditions only work for dialed in mono-tubs. If you're going un modded it's a bit different.
Ahh, thanks for that info! And yes, I'm working with mostly shoeboxes.
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ShaperDreaming
Weirdo



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin] 1
#25956339 - 04/26/19 08:50 AM (5 years, 8 months ago) |
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Quote:
nulls said:
Quote:
ShaperDreaming said: nulls, you got shoeboxes? Them surfaces are not going to look like the pictures from the "proper surface conditions" thread. Took me a min to realize those conditions only work for dialed in mono-tubs. If you're going un modded it's a bit different.
Ahh, thanks for that info! And yes, I'm working with mostly shoeboxes.
Shoeboxes are a bit more like a leap of faith. Don't be afraid to use your finger to test for saturation. Don't touch exposed grains or mushrooms, but a quick poke to the surface to feel if it's still damp, should be like poking a damp towel basically with nice spring back. But really it just takes some faith in knowing that you're set until your first flush finishes up. Leave that shit sealed the whole time, don't flip lids, don't fan, don't mist. Unless you're inspecting it for moisture just leave the damn lid on and latched until the last minute (or use bags like bod does).
I really need to put together my pseudo-casing tek... I'm spawning some shoe boxes this weekend, I'll try to put together a tek for how I prep my shoeboxes.
Quote:
Durgin said: Thanks everyone (even bod for classic bod snark). I tossed the tubs before reading these, but did harvest a fresh pound and a half from that mini mono beforehand so that makes me feel slightly better. I've slowed down for the moment but will take more pics in a few weeks when the next batch is ready to spawn.
And in the meantime, I'll enjoy the one second flush tub that's still going (there's a nice monster coming up and a couple dozen others) and leave my newest shoebox alone for the next week or so and hopefully that will go better.
Honestly, that'll get you where you're going until you grow more and get this figured out. So many people before even getting fruits, so you're doing more right than a lot of folks.
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Bph
Stranger



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25956560 - 04/26/19 10:52 AM (5 years, 8 months ago) |
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My advice is a total restart. Use it as an excuse to dive back into this hobby head first. Get yourself a flowhood thats how I handled it. Lol. Going to be going laminar this weekend! But one thing is for sure. "Gonnies never say die"
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Professor X
School for the Gifted


Registered: 04/18/19
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Bph]
#25956582 - 04/26/19 11:05 AM (5 years, 8 months ago) |
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I've started doing a coffee gypsum soak on my WBS and adding very to the jars, it's a little ugly but it works awesome to control moisture and eliminate bacteria which almost always prefaces trichoderma.
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ShaperDreaming
Weirdo



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Professor X] 1
#25956614 - 04/26/19 11:21 AM (5 years, 8 months ago) |
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Quote:
Professor X said: I've started doing a coffee gypsum soak on my WBS and adding very to the jars, it's a little ugly but it works awesome to control moisture and eliminate bacteria which almost always prefaces trichoderma.
What?! Where did you get this information about coffee eliminating bacteria?
If you're soaking your seeds before sterilization it doesn't matter what the hell you put into it... it comes out sterile, the bacteria will still get in if you put it there during inoculation. Coffee is super acidic and attracts lots of molds to it that grow way faster than mushroom mycelium. This is a risky option, especially if you're having problems with contam.
Seriously, stop spreading shit info all over these threads. Try citing some info and not just pumping out your personal thoughts w/o showing any proof that you know what you're doing. You're basically just making shit up.
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Professor X
School for the Gifted


Registered: 04/18/19
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25956851 - 04/26/19 01:25 PM (5 years, 8 months ago) |
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Quote:
ShaperDreaming said:
Quote:
Professor X said: I've started doing a coffee gypsum soak on my WBS and adding very to the jars, it's a little ugly but it works awesome to control moisture and eliminate bacteria which almost always prefaces trichoderma.
What?! Where did you get this information about coffee eliminating bacteria?
If you're soaking your seeds before sterilization it doesn't matter what the hell you put into it... it comes out sterile, the bacteria will still get in if you put it there during inoculation. Coffee is super acidic and attracts lots of molds to it that grow way faster than mushroom mycelium. This is a risky option, especially if you're having problems with contam.
Seriously, stop spreading shit info all over these threads. Try citing some info and not just pumping out your personal thoughts w/o showing any proof that you know what you're doing. You're basically just making shit up.
I use vermiculite for moisture control. I don't have contamination issues. I'm at a 0 percent contamination rate unless I do something stupid. As for shit info, you are the one telling people to open contaminated shit in their homes. Where did you find that TEK? I have personally read volumes on Mycology, and have never once read to take your contaminated jar to your SAB inside your home and open it. Wasn't there a guy not too long ago on this board that went to the ER after he opened a jar and gave it a little sniff. You can get as mad at me as you want but you are the one giving shit advice.
By the way, yes coffee is acidic and favors trich, that's why I use alkaline water to balance the PH, test strips confirmed. The mycelium loves it as well as the gypsum. I wasn't telling him to soak his millet in acidic slush I was telling him to use vermiculite in the millet jars to control moisture and fight contamination. I guess I'm just some idiot asshat child that spreads bad information. Sorry I didn't get that information from a tek, it came from Stephen Russell and Paul Stammets. Oh, I'm sure Roger Rabbit would agree, unless you have confirmed which of the 89 species of trichoderma you are dealing with through identification and taxonomy you should get it the hell out of your home like last week. Sure it could be a helpful plant symbiotic strain or it could be aggresivum which will almost surely require an entire home heat treatment similar to bed bug treatment you ever hope to grow any fungus other than it in your home again.
I agree a lot of fear mongering concerning contaminants takes place in this hobby/profession period but encouraging newbies to follow poor procedures that quite literally no one but you follows and is preached against to the point of force feeding is a major disservice not to mention dangerous (a member was hospitalized).
Any ways, I wholeheartedly disagree with your advice. You have a good day.
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ShaperDreaming
Weirdo



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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Professor X] 1
#25956905 - 04/26/19 01:52 PM (5 years, 8 months ago) |
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Quote:
Professor X said: As for shit info, you are the one telling people to open contaminated shit in their homes. Where did you find that TEK?
RR saying to transfer away from contam on a plate
RR saying it again 6 years later
Eagle's Gift, a TC saying it
Oh another TC saying it
Here's Munchauzen saying it
Bod saying it
Unless you can think of a way to transfer off agar without opening a dish... then maybe you got something going for you.
You can say you read everything in the world on the topic, doesn't make you right. Quote Stamets all you want, but he has a poor reputation here for a reason.
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Professor X
School for the Gifted


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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: ShaperDreaming]
#25957789 - 04/26/19 08:57 PM (5 years, 8 months ago) |
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We aren't talking about a plate, this guy has tubs full of green monster. Dude.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Professor X] 1
#25957831 - 04/26/19 09:27 PM (5 years, 8 months ago) |
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Oh so like this
 You dump it in your trash and wash it out in your shower with some soap and use it again
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: bodhisatta]
#25957877 - 04/26/19 09:56 PM (5 years, 8 months ago) |
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Quote:
bodhisatta said: Oh so like this
 You dump it in your trash and wash it out in your shower with some soap and use it again
For the record, they weren't nearly that bad, but I took them out to the deck and dumped in a trash bag and rinsed with a hose before throwing in the dishwasher to start fresh. So... Basically I'm ctrl-alt-deleting my last grows and will post pics of my next jars before spawning to double-check that it all looks right and then will take it from there.
In the meantime, I bought a proper dehydrator (based on bod's snarky-but-not-wrong critique of my use of my oven's dehydrate feature) and got a bit over an ounce cracker dry off the mini-mono. Also have a couple more dry ounces in the freezer (not the prettiest but definitely potent), and am expecting close to a dry ounce from the second flush of the one tub that's still going (the first two fruits were 33g wet, and there are a few dozen more fat ones still growing). So given that I've consumed maybe 10g dry in my entire life, I think I'll be ok for a while...
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Professor X
School for the Gifted


Registered: 04/18/19
Posts: 2,719
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Professor X]
#25957882 - 04/26/19 10:03 PM (5 years, 8 months ago) |
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By the way RR is the only one who says anything about transferring from mold. The rest are bacteria threads. Got a bacterial jar? Dump it and bleach the jar. Hell you can even birth it and get fruits sometimes. Moldy jar? Dispose of immediately. Like I said and correct me if I'm wrong but according to forum rules we are not supposed to hand out dangerous advice here. Opening a trichoderma culture that has not been positively identified is dangerous, some of the 89 species produce mycotoxins and are extremely dangerous to be around. The professionals wear hazmat suits when they deal with this shit. There's a poor guy in another thread right now that just keeps losing everything to green monster. Want to make a bet it started by opening a jar inside?
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25957883 - 04/26/19 10:04 PM (5 years, 8 months ago) |
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For what it's worth, this is the remaining shoebox on second flush, after harvesting two very decent fruits (33g combined wet) that started to open up before the rest:

Some of the sub looks like it might be a little moldy, but I haven't misted or bottom watered this one at all so it seems like it's not growing, and the fruits are totally clean. Going to ride it out for the time being and see what I can get from this flush, then after harvest may dunk briefly and place outside to see if I can get anything else out of it before it's a lost cause.
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Anoobus
Thrillionaire


Registered: 01/20/19
Posts: 242
Loc: Earth
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Durgin]
#25957936 - 04/26/19 10:30 PM (5 years, 8 months ago) |
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Agar will help you find your issue. That and a S.. A. B. BOD HAS A SERIES OF SIMPLE AF TEKS Aand pasty has a no pour agar tek. Pasty no pur agar link
Bods easy af teks link (Chek out Sab link)
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 8 months, 20 days
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Re: Trich during / after first flush on almost all my tubs - help me diagnose why? [Re: Anoobus]
#25957961 - 04/26/19 10:45 PM (5 years, 8 months ago) |
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Thanks, but see original post - I'm using agar and a SAB. (A couple of these tubs came from spawn that I started before that, but most were A2G in my SAB.)
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