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OfflineSquirellMaster
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Triche on wbs?
    #25947111 - 04/21/19 09:03 AM (5 years, 8 months ago)

I believe this is trich but I would like a confirmation. If it is how should I proceed? Go ahead and dump and start over? Thanks for your input friends.[url=http://files.shroomery.org/files/19-16/585889946-20190421_103750.jpg][image]http://www.shroomery.org/forums/thumbs/19-16/585889946-

Edited by SquirellMaster (04/21/19 09:06 AM)

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InvisibleShroomspective
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Re: Triche on wbs? [Re: SquirellMaster]
    #25947123 - 04/21/19 09:08 AM (5 years, 8 months ago)

Yup looks like it..failure of either sterilisation or your spores are contam. Ideally with grains you want to start off spores on agar, not directly inject jars

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OfflineSquirellMaster
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Re: Triche on wbs? [Re: Shroomspective]
    #25947130 - 04/21/19 09:15 AM (5 years, 8 months ago)

Thanks for the speedy reply. Agar would be ideal but gonna be impossible for me without a sab or flow hood. Used docs wbs tek and had alot of success in the past. Guess ima dumb this batch and start soaking again =)

Edited by SquirellMaster (04/21/19 09:15 AM)

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OfflineSquirellMaster
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Re: Triche on wbs? [Re: SquirellMaster]
    #25947143 - 04/21/19 09:25 AM (5 years, 8 months ago)

The syringe I used has no 0 problems on 10 cakes I knocked up the same day so I believe it to be a problem with sterilization. Apparently  a 12h soak and a 30minute simmer and 1h at 15psi was not enough?

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Re: Triche on wbs? [Re: SquirellMaster]
    #25947278 - 04/21/19 10:38 AM (5 years, 8 months ago)

No 60 mins is not enough..at least 90, and you need 10 mins clear venting with no weight on to ensure its hot enough before you start the clock, so closer to 120.

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OfflineWannaBScientist
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Re: Triche on wbs? [Re: Shroomspective]
    #25953862 - 04/24/19 11:52 PM (5 years, 8 months ago)

:thumbdown: like they said before me, try again a little longer next time

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