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 LorySF
Frog
Registered: 04/24/19
Posts: 8
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Last seen: 1 year, 8 months
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 No mycelium growth after 8 days 
#25952804 - 04/24/19 02:56 PM (1 year, 9 months ago) |
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I inoculated 4 1l wbs jars 8 days ago with a spore syringe that I bought in a reliable online shop and there's still no sign of mycelium growth. I keep them at around 20 °C in almost complete darkness.
Is this normal or should i worry?
P.S. English is not my mother tongue, please excuse any mistakes on my part
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Puduwoke
mushroom enthusiastic
Registered: 06/25/17
Posts: 1,657
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Re: No mycelium growth after 8 days [Re: LorySF]
#25952831 - 04/24/19 03:11 PM (1 year, 9 months ago) |
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Can take up to 3 weeks for spore to germinate, so I would not worry about that.
Colonizing in darkness is updated information tho.
No need to worry, oh also spores to grains is not recommended btw.
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LorySF
Frog
Registered: 04/24/19
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 Re: No mycelium growth after 8 days [Re: Puduwoke]
#25952841 - 04/24/19 03:17 PM (1 year, 9 months ago) |
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This is awesome news, thank you.
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WannaBScientist
Stranger
Registered: 01/02/19
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Re: No mycelium growth after 8 days [Re: LorySF]
#25953789 - 04/25/19 01:07 AM (1 year, 9 months ago) |
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you could also try to inoculate a small plate of agar or a form of BRF paste if you don't have access to agar.
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 Feasoghorm
Registered: 10/24/18
Posts: 2,915
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Dnt knw bout wbs but i've never had germination faster then 9 days with cakes. In my experience if you inject a shiteload of spore-nut it takes longer. If you shake the jars after you inject it cud take longer.
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LorySF
Frog
Registered: 04/24/19
Posts: 8
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Last seen: 1 year, 8 months
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Re: No mycelium growth after 8 days [Re: LorySF]
#25977996 - 05/07/19 04:19 PM (1 year, 9 months ago) |
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Now it's been 21 days since I inoculated the jars and there's still no visible mycelium growth (I even checked the inside by shaking them). I also started a LC a week ago and It didn't show any germination either.
Could the spore syringe I used be the problem? (I bought it from a company in the Shroomery vendor list)
Here are some pictures
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rizingfire
Mycoticus psychoticus
 
Registered: 09/10/07
Posts: 831
Loc: North-east USm'f'nA
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Re: No mycelium growth after 8 days [Re: LorySF]
#25978036 - 05/07/19 04:42 PM (1 year, 9 months ago) |
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LCs can be hit or miss...your grains may be too dry...I went back to brf for a while at one point cuz I had issues with wbs...try noccing up just 1 of em but put more than just a few drops and put em back...they didnt contaminate so that's at least a plus...
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Jxshy12
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Re: No mycelium growth after 8 days [Re: LorySF]
#25978052 - 05/07/19 04:51 PM (1 year, 9 months ago) |
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Spores can take 3 weeks or more sometimes to germinate, shaking them again when you didn’t see any growth probably hurt your chances a bit more
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DnDRnD
Hobby cultivator
Registered: 10/08/18
Posts: 2,887
Loc: Washington 
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Re: No mycelium growth after 8 days [Re: rizingfire]
#25978053 - 05/07/19 04:52 PM (1 year, 9 months ago) |
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What's your LC recipe? It could take awhile for spores to germinate (sometimes 2-3 weeks) spores directly to grain is highly advised against since syringes are inherently dirty, but when I did spores to grain (oats) I got growth in around 2 weeks or so.
Also give them some light, it's beneficial during all stages of growth
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LorySF
Frog
Registered: 04/24/19
Posts: 8
Loc:
Last seen: 1 year, 8 months
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Re: No mycelium growth after 8 days [Re: LorySF]
#25978098 - 05/07/19 05:26 PM (1 year, 9 months ago) |
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First of all thanks for your advise, anyways
1) I hydrated my grains for 24 hours, should be enough right?
2) I'll try to inoculate again one of the jars
3) It's been almost a month :/
4) I followed this guy's instructions
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DnDRnD
Hobby cultivator
Registered: 10/08/18
Posts: 2,887
Loc: Washington
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Re: No mycelium growth after 8 days [Re: LorySF]
#25978126 - 05/07/19 05:41 PM (1 year, 9 months ago) |
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Hmm, perhaps try a BRF cake just to see if the spores germinate or try some agar or BRF paste and see if they germinate
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JoshD
Stranger
Registered: 02/01/19
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Re: No mycelium growth after 8 days [Re: DnDRnD]
#25978213 - 05/07/19 06:28 PM (1 year, 9 months ago) |
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I'm new to this, but since the quality of the syringe is in question, wouldn't this be a good time for some agar work? A few petri dishes (or pasty plates for a DIY option) would be a good way to determine if the syringe is usable, wouldn't it?
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shroomify
Registered: 04/14/19
Posts: 51
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Re: No mycelium growth after 8 days [Re: JoshD]
#25980191 - 05/08/19 06:22 PM (1 year, 9 months ago) |
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Quite right, that's what I would do. Just remember only a single drop per dish will do. And cool the syringe after flame sterilisation by letting one drop go into your SAB floor.
Bear in mind that germination may take a while even in agar. Most likely a week, but sometimes it can take 2-3 weeks from what I have read.
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LorySF
Frog
Registered: 04/24/19
Posts: 8
Loc:
Last seen: 1 year, 8 months
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Re: No mycelium growth after 8 days [Re: LorySF]
#25982224 - 05/09/19 05:13 PM (1 year, 9 months ago) |
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Alright I'll try agar and I'll let you know how it goes
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