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OfflineLorySF
Frog
I'm a teapot
Registered: 04/24/19
Posts: 8
Loc: Flag
Last seen: 1 year, 8 months
No mycelium growth after 8 days
    #25952804 - 04/24/19 02:56 PM (1 year, 9 months ago)

I inoculated 4 1l wbs jars 8 days ago with a spore syringe that I bought in a reliable online shop and there's still no sign of mycelium growth. I keep them at around 20 °C in almost complete darkness.
Is this normal or should i worry?

P.S. English is not my mother tongue, please excuse any mistakes on my part


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OfflinePuduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 1,657
Last seen: 14 hours, 30 minutes
Re: No mycelium growth after 8 days [Re: LorySF]
    #25952831 - 04/24/19 03:11 PM (1 year, 9 months ago)

Can take up to 3 weeks for spore to germinate, so I would not worry about that.
Colonizing in darkness is updated information tho.

No need to worry, oh also spores to grains is not recommended btw.


--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021


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OfflineLorySF
Frog
I'm a teapot
Registered: 04/24/19
Posts: 8
Loc: Flag
Last seen: 1 year, 8 months
Re: No mycelium growth after 8 days [Re: Puduwoke]
    #25952841 - 04/24/19 03:17 PM (1 year, 9 months ago)

This is awesome news, thank you.


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OfflineWannaBScientist
Stranger
I'm a teapot

Registered: 01/02/19
Posts: 56
Last seen: 1 year, 8 months
Re: No mycelium growth after 8 days [Re: LorySF]
    #25953789 - 04/25/19 01:07 AM (1 year, 9 months ago)

you could also try to inoculate a small plate of agar or a form of BRF paste if you don't have access to agar.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 2,915
Re: No mycelium growth after 8 days [Re: WannaBScientist]
    #25953965 - 04/25/19 04:01 AM (1 year, 9 months ago)

Dnt knw bout wbs but i've never had germination faster then 9 days with cakes. In my experience if you inject a shiteload of spore-nut it takes longer. If you shake the jars after you inject it cud take longer.


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OfflineLorySF
Frog
I'm a teapot
Registered: 04/24/19
Posts: 8
Loc: Flag
Last seen: 1 year, 8 months
Re: No mycelium growth after 8 days [Re: LorySF]
    #25977996 - 05/07/19 04:19 PM (1 year, 9 months ago)

Now it's been 21 days since I inoculated the jars and there's still no visible mycelium growth (I even checked the inside by shaking them). I also started a LC a week ago and It didn't show any germination either.
Could the spore syringe I used be the problem? (I bought it from a company in the Shroomery vendor list)

Here are some pictures








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Offlinerizingfire
Mycoticus psychoticus
Male User Gallery


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Re: No mycelium growth after 8 days [Re: LorySF]
    #25978036 - 05/07/19 04:42 PM (1 year, 9 months ago)

LCs can be hit or miss...your grains may be too dry...I went back to brf for a while at one point cuz I had issues with wbs...try noccing up just 1 of em but put more than just a few drops and put em back...they didnt contaminate so that's at least a plus...


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OfflineJxshy12
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Registered: 03/27/19
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Re: No mycelium growth after 8 days [Re: LorySF]
    #25978052 - 05/07/19 04:51 PM (1 year, 9 months ago)

Spores can take 3 weeks or more sometimes to germinate, shaking them again when you didn’t see any growth probably hurt your chances a bit more


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OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,887
Loc: Washington Flag
Last seen: 3 months, 9 days
Re: No mycelium growth after 8 days [Re: rizingfire]
    #25978053 - 05/07/19 04:52 PM (1 year, 9 months ago)

What's your LC recipe? It could take awhile for spores to germinate (sometimes 2-3 weeks) spores directly to grain is highly advised against since syringes are inherently dirty, but when I did spores to grain (oats) I got growth in around 2 weeks or so.

Also give them some light, it's beneficial during all stages of growth


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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OfflineLorySF
Frog
I'm a teapot
Registered: 04/24/19
Posts: 8
Loc: Flag
Last seen: 1 year, 8 months
Re: No mycelium growth after 8 days [Re: LorySF]
    #25978098 - 05/07/19 05:26 PM (1 year, 9 months ago)

First of all thanks for your advise, anyways
1) I hydrated my grains for 24 hours, should be enough right?
2) I'll try to inoculate again one of the jars
3) It's been almost a month :/
4) I followed this guy's instructions


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OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,887
Loc: Washington Flag
Last seen: 3 months, 9 days
Re: No mycelium growth after 8 days [Re: LorySF]
    #25978126 - 05/07/19 05:41 PM (1 year, 9 months ago)

Hmm, perhaps try a BRF cake just to see if the spores germinate or try some agar or BRF paste and see if they germinate


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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OfflineJoshD
Stranger
Registered: 02/01/19
Posts: 5
Last seen: 1 year, 7 months
Re: No mycelium growth after 8 days [Re: DnDRnD]
    #25978213 - 05/07/19 06:28 PM (1 year, 9 months ago)

I'm new to this, but since the quality of the syringe is in question, wouldn't this be a good time for some agar work?  A few petri dishes (or pasty plates for a DIY option) would be a good way to determine if the syringe is usable, wouldn't it?


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Invisibleshroomify

Registered: 04/14/19
Posts: 51
Re: No mycelium growth after 8 days [Re: JoshD]
    #25980191 - 05/08/19 06:22 PM (1 year, 9 months ago)

Quite right, that's what I would do. Just remember only a single drop per dish will do. And cool the syringe after flame sterilisation by letting one drop go into your SAB floor.
Bear in mind that germination may take a while even in agar. Most likely a week, but sometimes it can take 2-3 weeks from what I have read.


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OfflineLorySF
Frog
I'm a teapot
Registered: 04/24/19
Posts: 8
Loc: Flag
Last seen: 1 year, 8 months
Re: No mycelium growth after 8 days [Re: LorySF]
    #25982224 - 05/09/19 05:13 PM (1 year, 9 months ago)

Alright I'll try agar and I'll let you know how it goes


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Shop: Bridgetown Botanicals Bridgetown Botanicals   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Capsules, Buy Kratom Extract, Kratom Powder For Sale   Amazon ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish, Wild Bird Seed

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