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Offlinethisbliss
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Registered: 02/27/19
Posts: 399
Last seen: 4 years, 7 months
Storing fruit tissue
    #25917221 - 04/05/19 09:44 AM (5 years, 9 months ago)

People how could I store a piece of mushroom meat so it would keep for at least a couple weeks and then I could clone it.

I'm waiting on agar equipment to arrive but I have a great cake fruiting a number of great flushes now that I think would be worthy of cloning.

I was thinking of just cutting a sterile section from the centre of a fruit and placing in a sterile jar. But this will probably mold in a week before I get to place on agar. Any ideas ? Thanks in advance

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OfflineMr. Funguy
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Registered: 01/19/19
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Re: Storing fruit tissue [Re: thisbliss]
    #25917257 - 04/05/19 10:04 AM (5 years, 9 months ago)

Freezer is my best guess...


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OfflineSFS96
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Re: Storing fruit tissue [Re: Mr. Funguy] * 1
    #25917263 - 04/05/19 10:06 AM (5 years, 9 months ago)

Id just wait untill u have the agar stuff in honesty :shrug:
Hopefully you will have some new fruits to clone by then.


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood

Edited by SFS96 (04/05/19 10:07 AM)

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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Storing fruit tissue [Re: SFS96]
    #25917286 - 04/05/19 10:20 AM (5 years, 9 months ago)

I would probably just wait as well. Maybe try some experiments with sterile water or something?

Dont freeze them either. When they start to thaw they’ll turn all nasty.


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Fail to plan and you plan to fail.

Enter the Ban Lottery

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Offlinethisbliss
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Registered: 02/27/19
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Re: Storing fruit tissue [Re: SFS96]
    #25917307 - 04/05/19 10:34 AM (5 years, 9 months ago)

True it's possible there'll be another flush after this one but cakes looking a bit spent.

I think I'll try the freezer sure why not. Also I could just put some in a new brf jar - that's like cloning right?

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InvisiblemushboyMDiscord
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Re: Storing fruit tissue [Re: thisbliss]
    #25917318 - 04/05/19 10:39 AM (5 years, 9 months ago)

freezing fresh shrooms will fuck with the cell structure. i dont think youll want to clone thawed out liquid mushroom goop.

just wait. there will always be more clones to take:awesome:

Quote:

thisbliss said:
Also I could just put some in a new brf jar - that's like cloning right?




you could but i wouldnt. just be patient.

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OfflineUncleHoyphae
Curious noob
I'm a teapot


Registered: 03/31/19
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Last seen: 5 years, 5 months
Re: Storing fruit tissue [Re: mushboy]
    #25917473 - 04/05/19 12:15 PM (5 years, 9 months ago)

Clone them now onto cardboard. Read up on the cardboard cloning tek, it's very simple and it does work. Once your agar arrives yu can transfer the healthy MYC from your cardboard to agar :smile:

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Offlinethisbliss
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Re: Storing fruit tissue [Re: UncleHoyphae]
    #25917639 - 04/05/19 01:37 PM (5 years, 9 months ago)

Thanks buds 👍

Must have a look at that cardboard.

Another thing I was wondering. Say I just let the cake sit about to keep the myc alive. Surely I can cut into the middle of it where it should still be sterile and take some myc from that? I know lot will probably frown at this but I'm thinking it might be a good project to learn on agar to clean it up - hone some skills

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OfflineUncleHoyphae
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I'm a teapot


Registered: 03/31/19
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Re: Storing fruit tissue [Re: thisbliss]
    #25918394 - 04/05/19 08:34 PM (5 years, 9 months ago)

I'm a noob so not really sure RE brf cakes. I've read that a cake can look super healthy on the outside but be contaminated in the middle when you cut it in half. I don't think the inside is necessarily sterile.

Oh and with the cardboard cloning tek. One of them talks about dipping in hydrogen peroxide first. DON'T do that. I tried and it killed all my samples and no MYC grew

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Offlinethisbliss
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Registered: 02/27/19
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Re: Storing fruit tissue [Re: UncleHoyphae]
    #25918718 - 04/06/19 01:15 AM (5 years, 9 months ago)

Sweet don't have any peroxide so was thinking of just skipping that stage lol


Might use the oven to sterilise some cardboard and then add sterile water to give it right consistency. Seems easiest. Should buy me a couple weeks

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OfflineUncleHoyphae
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Re: Storing fruit tissue [Re: thisbliss]
    #25919035 - 04/06/19 08:08 AM (5 years, 9 months ago)

Just boil it, put it in a small jar and then steam sterilise that 👍

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Offlinethisbliss
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Re: Storing fruit tissue [Re: UncleHoyphae]
    #25947093 - 04/21/19 08:37 AM (5 years, 8 months ago)

Unclehoyphae cheers dude for the heads up about the cardboard Tek. Got it going and managed to preserve enough mycelium to inoculate my agar plates when they arrived. Here's one plate I've done. I'm super proud it's my first forage into agar!

url=https://files.shroomery.org/files/19-16/585441696-IMG_20190421_143027746.jpg][image]http://www.shroomery.org/forums/thumbs/19-16/585441696-thumb_IMG_20190421_143027746.j

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Offlinethisbliss
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Registered: 02/27/19
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Last seen: 4 years, 7 months
Re: Storing fruit tissue [Re: thisbliss]
    #25947097 - 04/21/19 08:44 AM (5 years, 8 months ago)

Yep


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OfflineUncleHoyphae
Curious noob
I'm a teapot


Registered: 03/31/19
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Loc: Vietnam Flag
Last seen: 5 years, 5 months
Re: Storing fruit tissue [Re: thisbliss]
    #25948514 - 04/22/19 01:34 AM (5 years, 8 months ago)

Looks awesome dude! Well done :smile:

Got my first EVER pins today (been doing agar work with dried mushys for my first ever grow) and I'm super excited!


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Offlinethisbliss
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Registered: 02/27/19
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Re: Storing fruit tissue [Re: UncleHoyphae]
    #25948804 - 04/22/19 09:15 AM (5 years, 8 months ago)

👍 nice one. Some lovely mycelium action there. What you got going on in those tubs?

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OfflineUncleHoyphae
Curious noob
I'm a teapot


Registered: 03/31/19
Posts: 205
Loc: Vietnam Flag
Last seen: 5 years, 5 months
Re: Storing fruit tissue [Re: thisbliss]
    #25950288 - 04/23/19 04:53 AM (5 years, 8 months ago)

I'm just top fruiting a spawn jar cos I wanted to try get fruits quickly and with minimal risk before a 2 week trip beginning may. It's whole brown rice with a coir casing :smile:

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Offlinethisbliss
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Registered: 02/27/19
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Last seen: 4 years, 7 months
Re: Storing fruit tissue [Re: UncleHoyphae]
    #25950674 - 04/23/19 11:01 AM (5 years, 8 months ago)

Oh yeah? Lot of nice hyphae knots and pins there. Do you put a tub or plastic bag over the top to hold humidity?

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OfflineUncleHoyphae
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I'm a teapot


Registered: 03/31/19
Posts: 205
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Re: Storing fruit tissue [Re: thisbliss]
    #25950884 - 04/23/19 01:20 PM (5 years, 8 months ago)

Those ones are in a zip loc bag/an old plastic French bread stick bag. I also have some others inside 1l plastic containers with perlite at the bottom.

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Offlinethisbliss
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Registered: 02/27/19
Posts: 399
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Re: Storing fruit tissue [Re: UncleHoyphae]
    #25950972 - 04/23/19 02:12 PM (5 years, 8 months ago)

Good idea is that the french bag with the tiny holes in it? B perfect for fae. I use a lot of 2l soda bottles for FC among other things. Nearly best of the lot. Just a pita when fruits get big

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OfflineUncleHoyphae
Curious noob
I'm a teapot


Registered: 03/31/19
Posts: 205
Loc: Vietnam Flag
Last seen: 5 years, 5 months
Re: Storing fruit tissue [Re: thisbliss]
    #25952550 - 04/24/19 10:14 AM (5 years, 8 months ago)

Nah, just a long plastic bag type of thing really, but the perforated one would be a great idea.

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