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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Question regarding agar to grain vs grain to grain
    #25951767 -

Hello! I have reached the point where I've done 2 transfers, and have about 8 good looking agar plates with healthy mycelium. I'm a little unclear on how to proceed. I'm hoping to make 10 1qt grain jars for a monotub, but have the following questions.

1. Should I use my 8 agar plates to inoculate 10 jars?

2. Should I use the best looking agar plate to inoculate one jar then do grain to grain transfers from that jar? If so, what should I do with the other agar plates?

3. What is the best way to grow in perpetuity? Should I clone the best looking mushroom to agar, do some transfers, and repeat?

Thanks in advance for any advice!

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Re: Question regarding agar to grain vs grain to grain [Re: tigerbalm]
    #25951816 -

You do it however you like mang.
And yes, clone good.

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Re: Question regarding agar to grain vs grain to grain [Re: Feasoghorm]
    #25951827 -

Probably best to do A2Gs at this point. You need clean master jars for G2G and there's a high chance they won't be clean enough on your first attempt. With multiple A2G transfers you're more likely to have at least some jars succeed.


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How I do glass dishes

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Re: Question regarding agar to grain vs grain to grain [Re: poisoned]
    #25951840 -

Ah ok, so do agar to grain in 10 jars to get a good master, then use the good master for grain to grain? Also, how much of an agar plate do you use to inoculate a grain jar? I think I've heard cut it into 4 sections, is that accurate? Thanks a ton for the assistance!

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Re: Question regarding agar to grain vs grain to grain [Re: tigerbalm]
    #25951877 -

I dump entire plates in and just see what happens. I then plan to clone the best fruit from each jar.

But you can chop it into 4 or 8 or as small as you like. Smaller just may take a little longer to colonise.

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Re: Question regarding agar to grain vs grain to grain [Re: tigerbalm]
    #25951881 -

Agreeing with poisoned.  I would however take a tiny little wedge to transfer to new plates before dropping them to grain.
I use the whole plate in the jar but not everyone does.  Some use half, quarters, whatever you are comfortable with.


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Re: Question regarding agar to grain vs grain to grain [Re: SunnyDayze]
    #25951961 -

I drop two pucks in each qt

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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