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OfflineSmurfTurf
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ANTI-BACTERIAL substrate
    #25938263 - 04/16/19 03:17 PM (4 years, 10 months ago)

Ive done about 40-50 small grows over the last 10 years or so. I havent been very active on the forums, but I think its time to get back into it. Ill share my limited knowledge and hopefully someone can give me a decent answer to my question.

WHAT SUBSTANCE/CHEMICAL/DRUG CAN BE USED TO MAKE A SUBSTRATE ALMOST COMPLETELY ANTI-BACTERIAL AND NOT INHIBIT FUNGI GROWTH OR FRUITING?

I have tried many things. I dont have an issue with contaminations but, it would be a lot easier if things were just sterile and antibacterial from the beginning. It would open up a lot more outdoor grows and things like that.

Things I have tried with limited degrees of success.

Commercial agar with antibacterial mixture - slow growth
Tea extracts - no noticeable difference

.2% hydrogen peroxide in my humidifier(reptifogger) - Only works indoors, works very well, destroys the machine after 1000 hours - also really good for getting rid of chest colds if you inhale a few puffs, actually recommended to me by a doctor


I would like something that works outdoors that i can just mix in with the substrate and do log and basket teks etc.

Does anyone have any clue about a substance that can achieve this?


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InvisiblebodhisattaMDiscordReddit
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Re: ANTI-BACTERIAL substrate [Re: SmurfTurf] * 3
    #25938267 - 04/16/19 03:19 PM (4 years, 10 months ago)

Clean spawn is the magic additive you're looking for. You don't want to make super bacteria or any kind of resistance by inappropriately using antibiotics as a preventative. It also will help you not be able to notice yourself starting to have problems. You can't fix a systemic issue if you're using something that will cover it up.

Also in hospitals that use vaporized hydrogen peroxide  they work at 150-1500ppm but the safety literature puts 75ppm as critically dangerous to humans. Which means at even 10ppm it's probably still not good for you at all just not debilitating. At a concentration that doesn't cause immediate harm I would still imagine it's doing some harm. Don't breathe hydrogen peroxide your doctor is an idiot unless he pointed to compelling and peer reviewed research showing efficacy.

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OfflineFailboat
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Re: ANTI-BACTERIAL substrate [Re: bodhisatta]
    #25938292 - 04/16/19 03:36 PM (4 years, 10 months ago)

:horrified::canthelpbutlaugh:
Inhale a few puffs he said

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OfflineShaperDreaming
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Re: ANTI-BACTERIAL substrate [Re: Failboat]
    #25938335 - 04/16/19 03:59 PM (4 years, 10 months ago)

You can add Gentamicin Sulfate antibiotic powder to your agar to help you get that clean spawn bod is talking about.

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InvisibleAlchemycologist
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Re: ANTI-BACTERIAL substrate [Re: ShaperDreaming]
    #25938798 - 04/16/19 08:10 PM (4 years, 10 months ago)

You should only use gentamycin in extreme cases, such as a rare wild culture or something you can't replace. Like Bod said you'll end up breeding antibiotic-resistant bacteria and your problem will come back.

Clean spawn and properly prepared substrate (be it pasteurized or whatever) can be spawned outside or inside in open air.


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Yep.

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OfflineSFS96
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Re: ANTI-BACTERIAL substrate [Re: Alchemycologist]
    #25938835 - 04/16/19 08:24 PM (4 years, 10 months ago)

Clean spawn is key like stated. Shit i just picked some outdoor boomers and all i did was dump some clean spawn on the ground in a shady spot, then took a couple of cow parties and put them on top and watered. 3 weeks later after a heavy rain i had mushrooms. I didn’t pasturize the shit or anything. Now the mycelium has colonized most of the shit, im expecting more soon.


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood

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Offlinepoisoned
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Re: ANTI-BACTERIAL substrate [Re: SFS96]
    #25941772 - 04/18/19 03:00 PM (4 years, 10 months ago)

Quote:

SFS96 said:
cow parties



:psycow:


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How I do glass dishes

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OfflinePsilosopherr
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Re: ANTI-BACTERIAL substrate [Re: poisoned]
    #25944526 - 04/19/19 08:50 PM (4 years, 10 months ago)

I had this loaf of bread once that lasted months and months without molding and upon reading the label it had a food preservative thats not in most bread. Maybe try a food preservative

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Offlinesouthbounpachyderm
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Re: ANTI-BACTERIAL substrate [Re: Alchemycologist]
    #25944637 - 04/19/19 09:43 PM (4 years, 10 months ago)

Cant get gentamycin without a prescription anymore unfortunately :,( wanted to use some recently to clean up a wild print but no dice.


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InvisibleJozxyqk
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Re: ANTI-BACTERIAL substrate [Re: southbounpachyderm]
    #25944974 - 04/20/19 01:58 AM (4 years, 10 months ago)

There's a couple places you can get it still.  I just got some premixed agar with gentamycin from that dude who wrote a bunch of mushroom books. There's a sponsor here also who has it on their website, by the gram, or a 50 mg dose. There's an eBay listing for 100 g for around $100 which would last about 12 lifetimes, lol.

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Offlinesouthbounpachyderm
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Re: ANTI-BACTERIAL substrate [Re: Jozxyqk]
    #25945492 - 04/20/19 10:56 AM (4 years, 10 months ago)

Going on the hunt for that sponsor now! Thanks for letting me know :laugh:


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InvisiblebodhisattaMDiscordReddit
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Re: ANTI-BACTERIAL substrate [Re: southbounpachyderm]
    #25945513 - 04/20/19 11:08 AM (4 years, 10 months ago)

6+ years of growing and plenty of wild prints and clones and one thing ive never found a need for was antibiotics

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Offlinesouthbounpachyderm
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Re: ANTI-BACTERIAL substrate [Re: bodhisatta]
    #25945517 - 04/20/19 11:11 AM (4 years, 10 months ago)

Maybe one day I'll be there. Lots of patience in my future.


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InvisiblebodhisattaMDiscordReddit
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Re: ANTI-BACTERIAL substrate [Re: southbounpachyderm]
    #25945557 - 04/20/19 11:35 AM (4 years, 10 months ago)

Antibiotics don't help you get there. They keep you from learning really easy good technique. There's people their first post is agar grow log and canopy flushes. Imagine every kid that fell down learning how to ride a bike kept training wheels on for years. You fumble a few times then you ride forever.

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InvisibleJozxyqk
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Re: ANTI-BACTERIAL substrate [Re: bodhisatta]
    #25945660 - 04/20/19 12:36 PM (4 years, 10 months ago)

I have no disagreement with you there Bod. My post above may have sounded like I am pro-antibiotic but the opposite is true. After a couple of years of doing agar "the right way", I thought I'd give the antibiotics a try with a dirty syringe that I couldn't get to germ on agar.  As it turned out, the spores still did not germinate, and the bac grew as if there was no antibiotic present. I've had better luck with BRF with contaminated syringes.  Tried the gentamycin once, don't plan to use it again.  It's a crutch as you say, and the only legitimate use I thought would be what I had intended it for, but even then it didn't work. Indiscriminate use of antibiotics nearly killed my wife (not exaggerating) and has cost me hundreds of k's in the last five years due to the resultant health issues. So I'm about as anti-antibiotic as you can get. I was just commenting that it was still available without a prescription. As a public service announcement to all humans anywhere, if a doctor ever wants to prescribe you Clindamycin, run away as fast as you can. It is the go-to antibiotic for dentists and oral surgeons for some reason. C. difficile is resistant to it and God help you if you get an overgrowth of that in your guts. It will change your life forever, or possibly end it. Ok bye.

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OfflineSmurfTurf
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Re: ANTI-BACTERIAL substrate [Re: Failboat]
    #25949335 - 04/22/19 03:37 PM (4 years, 10 months ago)

Quote:

Quirkmeister92 said:
:horrified::canthelpbutlaugh:
Inhale a few puffs he said





LOL it sounds bad but its worked wonders for me during the winter


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OfflineSmurfTurf
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Re: ANTI-BACTERIAL substrate [Re: ShaperDreaming]
    #25949338 - 04/22/19 03:39 PM (4 years, 10 months ago)

Quote:

ShaperDreaming said:
You can add Gentamicin Sulfate antibiotic powder to your agar to help you get that clean spawn bod is talking about.





So clean spawn is spawn that was transferred from agar containing Gentamicin?

Or is clean spawn just really clean spawn?


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OfflineSmurfTurf
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Re: ANTI-BACTERIAL substrate [Re: Psilosopherr]
    #25949347 - 04/22/19 03:43 PM (4 years, 10 months ago)

Quote:

Psilosopherr said:
I had this loaf of bread once that lasted months and months without molding and upon reading the label it had a food preservative thats not in most bread. Maybe try a food preservative





That is a pretty interesting idea... if i ever come across something like that i will give it a shot.


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OfflineShaperDreaming
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Re: ANTI-BACTERIAL substrate [Re: SmurfTurf]
    #25949351 - 04/22/19 03:44 PM (4 years, 10 months ago)

Quote:

SmurfTurf said:
Quote:

ShaperDreaming said:
You can add Gentamicin Sulfate antibiotic powder to your agar to help you get that clean spawn bod is talking about.





So clean spawn is spawn that was transferred from agar containing Gentamicin?

Or is clean spawn just really clean spawn?



No, sorry, I was being sarcastic. As everyone is saying, no need to go that far. Clean spawn is spawn w/o any contaminant.

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