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Re: Help With Trich Outbreak [Re: Kizzle]
    #25946307 -

Yes lol, like the splatter pattern at the top right of the plate. The difference is mine are trich green with a white border. I'd take pics of that too but that the edges aren't wrapped. They're just confined to a baggie til I use them.

As for the mites, I have no idea. Years ago I had issues with gnats during fruiting and maggots in some jars but nothing like that in a very long time.

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Re: Help With Trich Outbreak [Re: mushboy]
    #25946318 -

GE isn't necessary the way I was using those. It's far more empty space than spawn. There's plenty of air to colonize a thin layer of grass seed or rice.

Think of it like an LC but it uses blended grain.

You are correct now. I wouldn't try it now because my unopened jars are contaminating. In the past it was a non-issue. I lost very few doing that method but I did learn the GE problem from having one of those nearly filled with rice stall.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25946328 -

Ara16w said:
Yes lol, like the splatter pattern at the top right of the plate. The difference is mine are trich green with a white border. I'd take pics of that too but that the edges aren't wrapped. They're just confined to a baggie til I use them.

As for the mites, I have no idea. Years ago I had issues with gnats during fruiting and maggots in some jars but nothing like that in a very long time.


Here's another one. I put a grain from a jar that didn't even show any mold. You can see the little trail leading away from the grain and the mold colonies it left behind.


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Re: Help With Trich Outbreak [Re: Kizzle]
    #25946340 -



There's a finished one I forgot about in the same container using the same method. The mushrooms pushed the lid off lol. It's either straight grass seed or grass seed and vermiculite.

Some of my methods may be a little unorthodox but I'm far from a noob. That's why when I say nothing works it's concerning. I could try 20 different methods on every grain imaginable for various cook times and still only grow trich now. It's not even a plethora of contaminates, just trich.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25946344 -

I hear yeah. I was regularly getting Trich after the 2nd flush. Since I moved out of that place, which was well over a year ago I haven't run into Trich even once.


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Re: Help With Trich Outbreak [Re: Kizzle]
    #25946368 -

I really hope moving out is not the answer to this problem.

Here's some more pics to further bastardize the status quo:




Guess which jar is most likely to be clean mycelium. The two on the right are trich pretending to be mycelium. They stink and have yellow myc piss in the bottom which will grow into a nice green substrate. Trust me, I know :rolleyes:

The one with the aluminum foil lid is ancient like Zeus. It was healthy myc on wbs that stalled when it reached the exploded grain on the bottom. It probably has verm in it too but it dried and it shrank. Will probably bury it.




That's the last of my healthy "can't be done spawn". There's zero point in trying to use it though if I'm just going to be mixing it with trich.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25946411 -

What kind of odor? Minty, coconut, dirt, sour, garbage... ? Spawn with bacterial contamination may contaminate with Trich shortly after spawning even if the Trich isn't actually in the jar itself.


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Re: Help With Trich Outbreak [Re: Kizzle]
    #25946427 -

Sour if I'm not mistaken. It's been a while since I checked them. Even the ones that smelt normal like mushrooms turned into trich after being spawned to manure in 3 weeks. Checked after 2, still smelt and looked normal, just weren't finished colonizing.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25947572 -

What kind of filter do you use on your furnace unit? Get the best filter you can and change it every 6 months for general air flow around your house. Good for you, and good for your shrooms :P You can get a serta air filter unit thing at lowes for fifty bucks right now too, Im sure they aren't godly or anything but I have one set up in the room I work in and it seems to help, might be a placebo affect though haha

The pressure cooker thing is a bit odd though, don't know what to tell you about that on.e


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Re: Help With Trich Outbreak [Re: Nikanoru]
    #25947673 -

We don't have functioning furnace unit. As mentioned in another post, this house is a cheap pos. Found this earlier behind layers of temporary insulation. Appears to be black mold I've since cleaned and am quarantining back off. There's damage to the underpinning and essentially a hole that's trapping moisture.



Surprisingly, I've grown no black mold even with my SAB a few feet from it, just trich.

I did have an activated carbon furnace filter on the back of a box fan to reduce the smell of smoke. Initially, I had it behind my trash bag and / or SAB without any problems. I don't know that it helped but it was pulling air away from my inoculation site.

The bottom line is I caused the trich problem. The first time I got it I didn't catch it in time. The additional 8 or so tubs going green and me being slow to catch it released an astronomical amount of trich spores. That's the only explanation I have for PCed uninoculated quart jars going green in a week.

I'm bleach / vinegar bombing my house and we'll see what happens I guess. It's been a huge pain in the ass.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25947705 -

I separated the "sneeze" plate to get a pic as to illustrate the spore load.



Makes little sense to me how I can get something that contaminated, completely clean, and mostly clean in the same stack of plates doing one after the other. The MS plate should be equally filthy but it's not.



Edited by Ara16w (04/21/19 02:45 PM)

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25957766 -

Ara16w said:
We don't have functioning furnace unit. As mentioned in another post, this house is a cheap pos. Found this earlier behind layers of temporary insulation. Appears to be black mold I've since cleaned and am quarantining back off. There's damage to the underpinning and essentially a hole that's trapping moisture.



Surprisingly, I've grown no black mold even with my SAB a few feet from it, just trich.

I did have an activated carbon furnace filter on the back of a box fan to reduce the smell of smoke. Initially, I had it behind my trash bag and / or SAB without any problems. I don't know that it helped but it was pulling air away from my inoculation site.

The bottom line is I caused the trich problem. The first time I got it I didn't catch it in time. The additional 8 or so tubs going green and me being slow to catch it released an astronomical amount of trich spores. That's the only explanation I have for PCed uninoculated quart jars going green in a week.

I'm bleach / vinegar bombing my house and we'll see what happens I guess. It's been a huge pain in the ass.


That’s Fucked! Im sorry dude good luck, maybe try covering it with a plastic sheet, or use antifungal paint. Pick a room and seal it with antifungal paint, make that your room for stuff only open the pc in there ect. You should still have a SAB or a flowhood would help the air. Maybe even a regular air scrubber and use glove box.

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Re: Help With Trich Outbreak [Re: Palmer1997]
    #25957786 -

An open air flowhood won't fix that. Situations like these are why you never open a contaminated jar in your home.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: Help With Trich Outbreak [Re: Professor X]
    #25958884 -

What got me was most likely bad spawn. I had 6 or so 8-10 quart substrate tubs go green on me at once and I didn't catch them til after they sporulated inside my mini GH. When I went to check if they were done, opened it, released it, and had to carry it to the other end of the house (closed of course). It was just a ridiculous amount of trich spores.

That mold spot had been there this whole time and hadn't caused me any issues that I've noticed. I made up a solution of 10% or less bleach water with an equal amount of vinegar added last and sprayed the entire house with it several days in a row. Also, mopped with it and got a new activated charcoal furnace filter on the back of a box fan that I let run on high for 2 days.

Hopefully, that's enough to get the problem under control.

Like I tried to say before, I was happy with my sterile technique. I've been doing this on and off for years and this last comeback I was having roughly 95% success rates with jars. The other indicator was that it was the first time I was having to throw old substrates away to make room that didn't contaminate (beyond a 5th flush). When I got all that trich I had 7 tubs like that in my mini GH that still didn't go green.

I have changed my SAB procedure a bit. I spray it down on the inside with that bleach vinegar solution to hopefully catch and explode mold spores rather than have them blow around every time I take an arm in or out of it.

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Re: Help With Trich Outbreak [Re: Ara16w] * 1
    #25958963 -

Ara16w said:
I have changed my SAB procedure a bit. I spray it down on the inside with that bleach vinegar solution


:facepalm:
You should of changed more. Bleach vinegar? Your SAB must stink:lol:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Help With Trich Outbreak [Re: mushboy] * 2
    #25958983 -

Don't be a retard never mix bleach and vinegar unless you have a fume hood and are not using it to clean with. Chlorine gas induced chemical pneumonia I've seen one time. The person wanted to kill himself from the agony that lasted for probably a week


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Help With Trich Outbreak [Re: mushboy]
    #25958984 -

mushboy said:
Ara16w said:
I have changed my SAB procedure a bit. I spray it down on the inside with that bleach vinegar solution


:facepalm:
You should of changed more. Bleach vinegar? Your SAB must stink:lol:


Chlorine gas: just as fragrant as it is toxic.  :thumbup:

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Re: Help With Trich Outbreak [Re: bodhisatta]
    #25959227 -

RogerRabbit said:
Search that phrase here for the many times I've typed exactly that.  It will kill most mold spores, but not live mycelium.

It helps after mixing a 10% bleach/water solution to add as much vinegar to the solution as you used in bleach.  The vinegar will reduce the pH to the point it will penetrate cell walls easier, increasing the sanitizing power.  However, it's still just a disinfectant, and much more effective against bacteria and virus than mold.
RR


https://www.shroomery.org/forums/showflat.php/Number/12927534#12927534

Learnt from the best. It was his advice in another thread that gave me the idea to do it the first place. It was stated after mass trich sporulation, that was all you could do.

I'd be a lot more concerned if it wasn't so diluted and actually produced any of the effects associated with chlorine gas.

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Re: Help With Trich Outbreak [Re: Ara16w]
    #25959233 -

Or dont spray the sab with weird chemicals? Completely unnecessary and borderline dangerous.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Help With Trich Outbreak [Re: mushboy]
    #25959243 -

RogerRabbit said:
To the original poster, spray the still-air-box inside with water and then use.  You don't need to wait for spore settling time.  The water you spray will knock the contaminants to the walls or floor of the box and hold them there.  Keep your flame on the outside of the box.  Pull your arm out to flame the tools and then re-insert and work.

Still air boxes are not sterile, nor is the air inside them sterile.  It's only a way to stop drafts while you work, quickly.
RR


Close the thread, it's ran its course. Dialogue only serves as an excuse for some of the people here to be assholes.

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