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OfflineMushtache
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Registered: 04/04/19
Posts: 3
Last seen: 4 years, 8 months
Agar Syringe Inoculation
    #25944278 - 04/19/19 06:18 PM (5 years, 8 months ago)

6 days ago I inoculated my first attempt at agar dishes with a spore syringe. At the two day mark, I could already see this contamination forming on all of the inoculated dishes.



I left three of the poured plates as control subjects and all three are crystal clear, so I believe it would be a fair conclusion that this was not my tek but that the syringes had too much contamination. I also inoculated some WBS jars a few weeks ago with another syringe from this vendor and after shaking them they have yet to reconsolidate. Originally I thought it was my tek but since this round with agar I'm lead to believe that all these syringes are not up to standard. If this is the case should I just throw these out and try the other syringes/vendors, or hope to see some kind of mycelium and try to purify it through transfers? If anyone could identify it or could share their way of moving forward from this, it would be greatly appreciated.

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InvisibleShroomspective
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Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Agar Syringe Inoculation [Re: Mushtache]
    #25944543 - 04/19/19 08:57 PM (5 years, 8 months ago)

Looks like your syringes are heavy bacterial..I'd buy some new ones from an alternate supplier

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