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OfflineSilentwolf88
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Can I rehydrate colonizing wbs with a humidifier?
    #25940925 - 04/18/19 03:31 AM (5 years, 8 months ago)

I have a couple jars that I shook at about 60-70% and haven’t shown any growth for about a week now.  They were colonizing very slow to begin with and I believe it’s because they’re too dry.  I would just restart but I’m out of the treasure coast LC, I won it from a giveaway and I’d really like to use the genetics. It hasn’t shown any signs of cotams, just very slow colonization. I decided to put the jars in a box with a humidifier, I filled it with distilled water and a bit of peroxide. Would this be a viable method to rehydrate them? The RH is at about 65 right now

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Silentwolf88] * 1
    #25940992 - 04/18/19 05:36 AM (5 years, 8 months ago)

No

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: bodhisatta] * 1
    #25941089 - 04/18/19 07:08 AM (5 years, 8 months ago)

You know, colonization has never stopped for me because of dry grains. In fact, it's better to have things dry. Kinda doubt that's the problem here. Need pics.


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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mad Season]
    #25941106 - 04/18/19 07:32 AM (5 years, 8 months ago)

:whathesaid:

If anything it's too wet.

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OfflineSFS96
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: bodhisatta]
    #25941219 - 04/18/19 09:17 AM (5 years, 8 months ago)

If u shook it a week ago and it still hasn’t recovered it’s probably contaminated. I shook a jar 2 days ago at 30% and today it’s already recovered :shrug: has nothing to do with how dry your grain is.


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Edited by SFS96 (04/18/19 09:18 AM)

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InvisibleMateja
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mad Season]
    #25941298 - 04/18/19 10:16 AM (5 years, 8 months ago)

Quote:

Mad Season said:
You know, colonization has never stopped for me because of dry grains. In fact, it's better to have things dry. Kinda doubt that's the problem here. Need pics.



Idk if anyone remembers these two experiments I did concerning colonization times and water content?

WBS experiment https://www.shroomery.org/forums/showflat.php/Number/24824234
Quote:

Mateah said:
Quart jars, 7h room temp soak, 5ml LI, inoculated Nov 21, shaken Nov 29 (I wrote on the jars "shaken 19/11" but its suppose to say 29/11" (I also wrote "shook") :facepalm:  :bigweed:


Quart jars, 48h room temp soak, 5ml LI, inoculated Nov 21, shaken Nov 29


Quart jars, 60 min hot soak, 5ml LI, inoculated Nov 21, shaken Nov 29

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------



300ml jars 7h room temp soak, 1ml LI, inoculated Nov 21


300ml jars 48h room temp soak, 1ml LI, inoculated Nov 21


300ml jars 60 min hot soak, 1ml LI, inoculated Nov 21
[/url]






And then BRF cakes https://www.shroomery.org/forums/showflat.php/Number/24834832
Quote:

Mateah said:
24 BRF cakes were made, 12 with 4:1:2 ratio and 12 with 2:1:1 ratio (verm, water, flour)

For batch one I used 12dl vermiculite, 3dl water and 6dl brown rice flour. (4:1:2)
For batch two I used 12dl vermiculite, 6dl water and 6dl brown rice flour. (2:1:1)

This test is determine if colonization times will be affected by the different ratios of water.
All jars were inoculated with 1ml blenderless LI each, a clone was used. Inoculated on Dec 2

6 days after inoculation
These the dryer BRF cakes made with 4:1:2 ratio      and these are the regular PF cakes with 2:1:1 ratio
                             



A few days down the road
2:1:4 ratio


2:1:1 ratio







The data from my experiments shows that myc prefers wetter over drier, at least in this experiment.

Has anyone else done similar tests with clones?


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Cakes inside Water Tub

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Offlineverum subsequentis
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25941321 - 04/18/19 10:38 AM (5 years, 8 months ago)

I have had wbs stall out because it was to dry. I know because i thought it was to dry when i loaded it, it stalled out, I injected some sterile water and it took back off and did fine. I'll agree that most people don't run into grain that is too dry. Most newer folks make their grain on the wet side. BUT, it can happen.

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: verum subsequentis]
    #25941355 - 04/18/19 11:06 AM (5 years, 8 months ago)

It seemed to me at first like Mad was referring to colonization times being better with drier grain
but now I read it as he was saying (implying) that from a bacterial spreading perspective its better being drier?
I'm not totally sure what he meant tho (what I misinterpreted) :typing:


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Cakes inside Water Tub

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25941402 - 04/18/19 11:35 AM (5 years, 8 months ago)

Sorry I meant to say it's better to have grains more on the dry side than risking things being too wet. Grains are rarely too dry for colonization. Maybe like the top in some cases, but nothing a shake shouldn't fix.


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OfflineSilentwolf88
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Silentwolf88]
    #25941695 - 04/18/19 02:08 PM (5 years, 8 months ago)

Thanks for all the replies. The reason I think it could be a bit too dried out is because I have a sort of faulty PC, I realized after an hour of it just boiling and not reaching pressure that I have to tape down the handles for it to pressurize correctly. All the water evaporated and I had to refill it, so I PC’d it correctly for another 90 minutes after that.

These are the before and after pics

Edited by Silentwolf88 (04/18/19 02:09 PM)

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OfflineSilentwolf88
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25942048 - 04/18/19 05:21 PM (5 years, 8 months ago)

Those dryer ones definitely resemble the growth of mine, did they end up stalling at all?

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Silentwolf88]
    #25942074 - 04/18/19 05:39 PM (5 years, 8 months ago)

The dry cakes finished a week later.


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OfflineSilentwolf88
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25942142 - 04/18/19 06:13 PM (5 years, 8 months ago)

Ah ok, well the jar in the picture recovered well the first shake, it was after the second that it started to show signs of no progress.  I was told I need to shake it at 30% and then once more at 70%

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Silentwolf88]
    #25942191 - 04/18/19 06:38 PM (5 years, 8 months ago)

Sometimes an additional shake will help speed up full colonization and sometimes it will not.
If I for example have a patch of uncolonized grain the size of a pingpong ball or two in the spawn jar after mostly being colonized and if the uncolonized grain seems kind of dry then I know from experience it take a week for that patch to finish. But breaking the culture up an additional time at >90% full instead of letting it to continue to crawl onto the grain slowly, then the culture will usually regenerate evenly all over in 2-3 days tops and finish full colonization.


Make lots of spawn jars and experiment with different routines :super:


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OfflineFungshoo
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25942251 - 04/18/19 07:15 PM (5 years, 8 months ago)

Mateah that kinda is along the lines with the post i just made,
i didnt dunk my cakes going to bulk and now im kinda wishing i did.
i guess i could just mist a bit more.

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Fungshoo]
    #25942281 - 04/18/19 07:31 PM (5 years, 8 months ago)

Don't dunk the cakes if you're about to spawn them, you'll only end up with a
lower volume spawn and it will be all slushy and it will recover slower if spawned wet and smooshy.


I'd recommend grating the cakes 'dry' and on the finest grating side of the cheese grater (goal=entropy) more inoculation points = faster colonization of bulk sub and a faster overall sub IME.


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OfflineSilentwolf88
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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Mateja]
    #25942352 - 04/18/19 08:03 PM (5 years, 8 months ago)

Thanks for the info! I’m really hoping the humidity could help it get back up to pace.. I live in a real dry climate to begin with so I’m hoping that keeping them around 80% RH box could give them another chance to grow.

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Re: Can I rehydrate colonizing wbs with a humidifier? [Re: Silentwolf88]
    #25942440 - 04/18/19 08:44 PM (5 years, 8 months ago)

For Grain jars and BRF cakes to evaporate off so quickly that they dry out ANY considerable amount in a relatively short time would almost defy the concept of 'sealing' inside properly functioning containers made for specifically sealing lol :shrug:

Sealed substrates and grains don't just dry out like that. But I did have a couple of qt WBS jars dry out long time ago back when I had a 1inch hole in the lid stuffed not so tightly with polyfil. And I haven't heard of anyone experiencing problems with this ever so I wouldn't worry :super:


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Cakes inside Water Tub

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