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OfflineIndigoSpirit
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Contamination?
    #25938822 - 04/16/19 08:17 PM (5 years, 8 months ago)

Is there ANY chance this is not contamination? And if it is, could it be due to the syringe spores themselves? Because I boiled, I made the syringe red hot, I used alcohol on my hands and light and tools, etc. I don't think I could have done anything else to take the necessary precautions.


https://drive.google.com/file/d/1QmYDPAGD7oWvmyMVoCnqh2xig1eFGk5G/view?usp=sharing

https://drive.google.com/file/d/1Ql0auXaOrbXv7uvqx1Yz2ZNrjETguXlw/view?usp=sharing

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OfflineEl Chupacabra
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Re: Contamination? [Re: IndigoSpirit]
    #25938841 - 04/16/19 08:26 PM (5 years, 8 months ago)

Nah that's trich, or something else that looks a lot like trich to my untrained eyes. Either way it is contaminated.
Is that just a glass cup with a ghetto foil lid? Did you add a dry verm layer?

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OfflineIndigoSpirit
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Re: Contamination? [Re: El Chupacabra]
    #25938853 - 04/16/19 08:33 PM (5 years, 8 months ago)

I did add a dry verm layer. And yes it is a ghetto tin foil top lol. What do you recommend using instead?

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OfflineEl Chupacabra
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Re: Contamination? [Re: IndigoSpirit]
    #25938880 - 04/16/19 08:49 PM (5 years, 8 months ago)

If you can find half pint jars and modify the lids for pf tek that would be best. I have to wonder if the jar(s) were steamed or sterilized properly anyway. Could also have been a filthy syringe too.
There's a few less than ideal factors that could have given you contams.

I would get the half pint jars and get a spore syringe from a sponsor and follow the pf Tek to a T and you will likely have good results.

Do you by chance live in the UK?

EDIT: check your private messages too.

Edited by El Chupacabra (04/16/19 08:51 PM)

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OfflineJtimlin
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Re: Contamination? [Re: El Chupacabra]
    #25938931 - 04/16/19 09:17 PM (5 years, 8 months ago)

If using the PF Tek method, is there a reason that I shouldn't wait until after cooking the jars to poke the holes in the lids? I would, of course, sterilize the nail.

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OfflineEl Chupacabra
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Re: Contamination? [Re: Jtimlin]
    #25938958 - 04/16/19 09:34 PM (5 years, 8 months ago)

Quote:

Jtimlin said:
If using the PF Tek method, is there a reason that I shouldn't wait until after cooking the jars to poke the holes in the lids? I would, of course, sterilize the nail.




4 holes should already be in the jar lids before getting steamed/PC'd. The jars should get loaded with the BRF mixture and a dry verm layer on top. Lids are then put on, usually upside down because it's easier to take off later down the road. Then it gets a piece of foil over that to keep your jars safe during steaming/PCing.

It just sounds like it would be bad practice doing the holes after. You wanna leave that dry verm layer as undisturbed as possible and you already have to disturb it 4 times anyway with inoculations. The tops of the lids won't be sterile so if you drive a sterile nail thru it then it's not sterile anymore and you can push nasties in... Then when you inoc you might drive the nasties beyond the dry verm filter.:shrug:

I'm trying to not talk out of my ass too, I haven't done brf cakes in years. Maybe someone else can chime in with a better reason why that's bad practice.

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OfflineJtimlin
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Re: Contamination? [Re: El Chupacabra]
    #25939048 - 04/16/19 10:47 PM (5 years, 8 months ago)

Thanks for the response. I thought that I might wipe the top of the lids with alcohol to prevent as much contamination as possible. I'm going to try it on some of the jars and see if it works. I'll re-post how it goes. I'm going to feel pretty silly if they blow up. Lol.

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OfflineEl Chupacabra
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Re: Contamination? [Re: Jtimlin]
    #25939385 - 04/17/19 06:14 AM (5 years, 8 months ago)

Quote:

Jtimlin said:
Thanks for the response. I thought that I might wipe the top of the lids with alcohol to prevent as much contamination as possible. I'm going to try it on some of the jars and see if it works. I'll re-post how it goes. I'm going to feel pretty silly if they blow up. Lol.



Yea they probably will pull a vacuum, maybe if you do the upside down trick it MAY not. It seems so risky I just don't understand why you would do it in that order.
If you do it that way anyway I would love to see updates tho.

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OfflinePumpJackTeX
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Re: Contamination? [Re: El Chupacabra]
    #25939441 - 04/17/19 07:19 AM (5 years, 8 months ago)

I would recommend you stop boiling your syringes & purchase some from * until you have more experince & this should prevent your problem as long as you dont mess up the inoculation but this will narrow down your problems significantly.


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Edited by bodhisatta (04/17/19 10:45 AM)

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OfflineEl Chupacabra
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Re: Contamination? [Re: PumpJackTeX]
    #25939487 - 04/17/19 07:46 AM (5 years, 8 months ago)

Quote:

TexXx said:
I would recommend you stop boiling your syringes & purchase some from ------- until you have more experince & this should prevent your problem as long as you dont mess up the inoculation but this will narrow down your problems significantly.




Agreed, but also I would recommend not mentioning specific vendors or sponsors by name... Might wanna edit that bud.

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OfflinePumpJackTeX
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Re: Contamination? [Re: El Chupacabra]
    #25939565 - 04/17/19 09:09 AM (5 years, 8 months ago)

What kinda of Nazi shit is going on here in 2019?


I have talked about them since 2008 and 11 years later it's a fucking problem but my city is voting to legalize mushrooms while spores are complety legal.


Man I'm so fucking done with this website.


Good fucking job Yathan


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Ascension Energy | UFOs | 2021

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OfflinePumpJackTeX
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Re: Contamination? *DELETED* [Re: El Chupacabra]
    #25939568 - 04/17/19 09:12 AM (5 years, 8 months ago)

Post deleted by bodhisatta

Reason for deletion: Ignorance


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Edited by bodhisatta (04/17/19 10:28 AM)

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OfflineEl Chupacabra
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Re: Contamination? [Re: PumpJackTeX]
    #25939635 - 04/17/19 10:05 AM (5 years, 8 months ago)

Calm down dude. Smoke a damn bowl or something.
It's a RULE here and has been that way since I can remember. You should know that since you've been here for 11 years.
* You dont mention sponsors in mushroom cult.
It's kinda like courtesy my man, spores are for microscopy technically. Thats how we stay on the right side of the law.
Quote:

3.  Spores of active species are sold for microscopy use only so mycologists can ID mushrooms. Cultivation supplies are for legitimate legal growing. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. No one needs to know where you got your spores, kit, or other supplies.




I was even nice about it. Can't say the same about you with responses like that. I can't ban nor would I but if I were a mod I would edit that bullshit for you and dish out a warning.

Theres just no need to conduct yourself like this. We're all here to help one another not to attack one another.

Edited by El Chupacabra (04/17/19 10:08 AM)

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OfflineIndigoSpirit
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Re: Contamination? [Re: El Chupacabra]
    #25939642 - 04/17/19 10:08 AM (5 years, 8 months ago)

Thanks buddy. No, I live in Canada. I ordered from the 101 website in canada...won't specify exact name because i don't think we're supposed to given the previous messages, but 101 should give enough of a hint. And from the sounds of things many people have had bad experiences with them.

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OfflineSFS96
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Re: Contamination? [Re: El Chupacabra]
    #25939643 - 04/17/19 10:11 AM (5 years, 8 months ago)

What the fuck?:whathesaid: Dude why do u think they label prints for microscopy only? There is a fourm for discussing vendors. U don’t need to put the sponsors on blast in a cultivation fourm :shrug:


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InvisibleMad Season
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Re: Contamination? [Re: PumpJackTeX]
    #25939812 - 04/17/19 11:55 AM (5 years, 8 months ago)

Quote:

TexXx said:
Man I'm so fucking done with this website.


Good fucking job Yathan



A great man was lost today :lmafo:


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OfflineCorundum
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Re: Contamination? [Re: Mad Season]
    #25939896 - 04/17/19 12:42 PM (5 years, 8 months ago)

I am tempted to say that your use of tin foil instead of a mason jar lid might be contributing to your contam here


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OfflineJtimlin
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Re: Contamination? [Re: El Chupacabra]
    #25940248 - 04/17/19 04:27 PM (5 years, 8 months ago)

Lol. My wife is trying to apply principles of home canning, by letting the jars build pressure the contents can reach higher temps and therefore be more likely to succesfully sterilize. I was hesitant to change my steps, but could think of no honest reason why. I was curious if anyone could think of something we hadn't. We are going to try this method and I'll definitely post updates. Thanks again for the feedback.

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