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Offlineeagle12
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Registered: 04/11/19
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Spore syringe pf tek help
    #25939044 - 04/16/19 10:46 PM (5 years, 8 months ago)

Room is 75 degrees half pint jars are in darkness. how many days should it be for me to start seeing growth. Inserted spore syringe 1cc to e

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OfflineStrohngBahd
Not The Cheat
I'm a teapot


Registered: 04/15/19
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Re: Spore syringe pf tek help [Re: eagle12]
    #25939092 - 04/16/19 11:11 PM (5 years, 8 months ago)

How long has it been? I've ranged from 1-3 weeks to seeing growth.  Coming from a noob though, also how did you prep the jars?


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No Turn Un-Stoned

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OfflineSFS96
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Re: Spore syringe pf tek help [Re: StrohngBahd] * 1
    #25939107 - 04/16/19 11:19 PM (5 years, 8 months ago)

Could be a couple of days could be a week or so. Just leave them and don’t look at them for a week. Also mycelium needs light at all stages. It’s outdated info that they need to be kept in the dark.


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OfflineFleeceManeLion
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Re: Spore syringe pf tek help [Re: SFS96]
    #25939118 - 04/16/19 11:30 PM (5 years, 8 months ago)

Took about 3 or 4 days for me to see any growth. This was with a small amount of indirect sunlight.

Edited by FleeceManeLion (04/16/19 11:30 PM)

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OfflineRJ Tubs 202
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Re: Spore syringe pf tek help [Re: StrohngBahd]
    #25939122 - 04/16/19 11:34 PM (5 years, 8 months ago)

Quote:

StrohngBahd said:

. . . how did you prep the jars?




:rockon:

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OfflineSingularFusion
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Re: Spore syringe pf tek help [Re: RJ Tubs 202]
    #25939201 - 04/17/19 01:34 AM (5 years, 8 months ago)

I just started cultivating, only done three varieties in pf jars, but I would agree so far that you can put them away for a week and not look at them... and everything else said above

patience is key, and the more you look at them, turn them, move them around etc, the more chance something bad is introduced to them and they end up contaminated, or that if one is contam'd already somehow during the process, you accidentally spread something to the others

best of luck with it

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OfflineJQM
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Re: Spore syringe pf tek help [Re: SingularFusion] * 1
    #25939215 - 04/17/19 01:52 AM (5 years, 8 months ago)

inoculate, then don't look at them for 10 days to 2 weeks. Then check for contains and remove contaminated jars, if any, and find a good use for them elsewhere. Then just every 2 days when you're around 80%. Then every day around 95%. Fruit either after the first pins, or after 100%. Just my plan of attack to not get antsy.

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Offlineeagle12
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Re: Spore syringe pf tek help [Re: StrohngBahd]
    #25939556 - 04/17/19 09:03 AM (5 years, 8 months ago)

Quote:

StrohngBahd said:
How long has it been? I've ranged from 1-3 weeks to seeing growth.  Coming from a noob though, also how did you prep the jars?


it's been 2 days. For me.  Bed cakes in half pint jars half in dry verm on top.  Some jars have 2 holes some jars have 4.

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OfflineStrohngBahd
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Re: Spore syringe pf tek help [Re: eagle12]
    #25939597 - 04/17/19 09:32 AM (5 years, 8 months ago)

I've been out of the loop for a decade until recently, my first batch of cakes didn't show any myc until 3 weeks after innoc, was before I got a PC so I was steam cleaning.  2nd batch I made too wet I believe, will be 3 weeks tomorrow and I haven't seen anything, but its PE which I've heard can take a long time.  3rd batch I got the cake mix right and pressure cooked these jars and one of them is showing myc growth on the 6th day. 

As others have said, leave them be for the most part, give them time and keep an eye out for contams! Good luck!


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OfflineCorundum
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Re: Spore syringe pf tek help [Re: StrohngBahd] * 1
    #25939883 - 04/17/19 12:32 PM (5 years, 8 months ago)

Quote:

eagle12 said:
Quote:

StrohngBahd said:
How long has it been? I've ranged from 1-3 weeks to seeing growth.  Coming from a noob though, also how did you prep the jars?


it's been 2 days. For me.  Bed cakes in half pint jars half in dry verm on top.  Some jars have 2 holes some jars have 4.



2 days is not panic time yet. It can take a while for cube myc to start showing. I agree with JQM and Strongbahd in that its best to just leave them for a while because after you inoculate there's nothing you can really do to accelerate the process. If they do contam you will want to dispose of them, though.

Quote:

StrohngBahd said:
I've been out of the loop for a decade until recently, my first batch of cakes didn't show any myc until 3 weeks after innoc, was before I got a PC so I was steam cleaning.  2nd batch I made too wet I believe, will be 3 weeks tomorrow and I haven't seen anything, but its PE which I've heard can take a long time.  3rd batch I got the cake mix right and pressure cooked these jars and one of them is showing myc growth on the 6th day. 

As others have said, leave them be for the most part, give them time and keep an eye out for contams! Good luck!



I have experience only with PF since I have not taken the plunge to get a pressure cooker yet. 3 weeks is way too long to see progress on a PF jar. Jars are usually done or close to being done at 3 weeks. At that point I would be unsure that what I had in my jar was even cube myc.


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:samus::samus:

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InvisibleAlchemycologist
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Registered: 04/09/19
Posts: 285
Re: Spore syringe pf tek help [Re: Corundum]
    #25939998 - 04/17/19 01:47 PM (5 years, 8 months ago)

It can take 2-3 weeks for Cubensis spores to germinate. That's not weird.

Just wait. It's all you can do anyway.


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Yep.

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OfflineStrohngBahd
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Re: Spore syringe pf tek help [Re: Alchemycologist]
    #25940069 - 04/17/19 02:23 PM (5 years, 8 months ago)

Well the PE jars I prepped pretty badly.  I don't really have hope for them but they haven't shown any bacterial growth either yet so I'm leaving them be.  I just got started with agar so I'm gonna try the PE on plates and see.  The other syringes I got from the same vendor are definitely good.  Time will tell for the PE!


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OfflineCorundum
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Re: Spore syringe pf tek help [Re: Alchemycologist]
    #25940279 - 04/17/19 04:52 PM (5 years, 8 months ago)

Quote:

Alchemycologist said:
It can take 2-3 weeks for Cubensis spores to germinate. That's not weird.

Just wait. It's all you can do anyway.



Given 2/4 inoculation points per jar, the average jar would germinate by then. Seeing absolutely no progress by cube myc would make me think that some kind of contaminant is likely present. It's not impossible but given my experience inoculating hundreds of jars it can be a sign that something isn't going right, and I wouldn't call it normal.


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:samus::samus:

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InvisibleAlchemycologist
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Posts: 285
Re: Spore syringe pf tek help [Re: Corundum]
    #25940511 - 04/17/19 07:33 PM (5 years, 8 months ago)

Well I may have a new registration date but I've been at this for 16 years. I've done thousands of plates and jars and LC and everything else. I know anyone could claim that but it's true. My old account was 36fuckin5 if you wanna look it up, I just forgot the password.

Cube spores can take weeks to germinate. It's uncommon but it happens, I promise. Do some searching and you'll see.


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Yep.

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OfflineCorundum
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Re: Spore syringe pf tek help [Re: Alchemycologist]
    #25941932 - 04/18/19 04:10 PM (5 years, 8 months ago)

Quote:

Alchemycologist said:
Well I may have a new registration date but I've been at this for 16 years. I've done thousands of plates and jars and LC and everything else. I know anyone could claim that but it's true. My old account was 36fuckin5 if you wanna look it up, I just forgot the password.

Cube spores can take weeks to germinate. It's uncommon but it happens, I promise. Do some searching and you'll see.



That's exactly what I was saying, though. It's uncommon. Another word for that is weird, bud.


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:samus::samus:

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OfflineMushroomGrub
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Registered: 04/16/19
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Re: Spore syringe pf tek help [Re: SFS96]
    #25941946 - 04/18/19 04:25 PM (5 years, 8 months ago)

Quote:

SFS96 said:
Could be a couple of days could be a week or so. Just leave them and don’t look at them for a week. Also mycelium needs light at all stages. It’s outdated info that they need to be kept in the dark.




Just wanted to respond to this from a quote from RogerRabbit that suggests keeping Mycelium in the dark when colonizing w/ bulk substrate (to discourage pre-emptive pinning):

"The time to protect from light is during spawn run of bulk substrates and casing layers. During the initial colonization of grains or brf cakes, it doesn't matter. Light is required for primordia formation as well. Bulk substrate colonization is the last step prior to fruiting. The only reason for keeping a bulk substrate, and especially the casing layer dark during colonization is for the timing of the pinset initiation. It allows you to introduce all the major pinning triggers simultaneously, resulting in an explosion of pins. A bulk substrate will colonize just fine if exposed to light from day one, but then you aren't maximizing potential by synchronizing the pinning triggers"

Find the quote in the lighting megathread here:
https://www.shroomery.org/forums/showflat.php/Number/12406127#124061277


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