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Offlinesporesandsuch
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: bodhisatta]
    #25934024 - 04/14/19 01:22 PM (11 days, 25 minutes ago)

Quote:

bodhisatta said:
...
cakes just take a long time sometimes




Understood, thanks. I think I'll do grain jars next, and am looking forward to kickstarting the next set with agar cultures instead. Our organic chicken feed looks like it could be an awesome medium: cracked corn, whole millet.

Anyway, any general tips on *spawning* in the high desert, whether it's jars, bags, or whatever? (I'm pretty sure I've got a good solution for FAE & humidity in my fruiting chamber.)


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InvisiblebodhisattaM
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: sporesandsuch]
    #25934035 - 04/14/19 01:28 PM (11 days, 20 minutes ago)

Worst case run a humidifier in the room. Will help your energy bill those days the ac is stuck on


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Offlinesminklejoe
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: bodhisatta]
    #25936236 - 04/15/19 03:51 PM (9 days, 21 hours ago)

Hello Bodhisatta! New here on the forum, but been reading your posts for a while. Great stuff and much appreciated. Figured I was gonna try out a laminar flow build. Live in Northern Europe so not too easy to get a hold on a HEPA, but I got one of the Astrocel HCX I filters, which apparently were built to allow a larger air flow (which might be a problem in my case?)

Asked the company for a graph like the one you show in your easy AF guide, this is the reply:
"This is what our product manager said, "At 400 CFM the initial resistance of a 18 x 24 x 11.5 AstroCel I HCX would be approx. 0.3 in wg.  At 200 CFM the initial resistance will be approx. 0.14 in wg." We hope this helps!"

Thats a very low resistance, you said the filter should have about 0.8 WG at desired flow rate. Is this filter useless for this purpose then, or could I still make use of it?

Thanks, best regards, Sminklejoe

EDIT: Might it be so easy I just need a smaller fan?


Edited by sminklejoe (04/15/19 04:00 PM)


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OfflineJakeoncid419
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: sminklejoe]
    #25937950 - 04/16/19 02:09 PM (8 days, 23 hours ago)

So I’m trying to come up with a system to take large amounts of sterile prints. I suppose one way to do it is make a few sab’s just for printing. (My flowhood is small) the other thing I was thinking about doing is attaching a hepa filter fan I have to an expansion tub (so I can branch off multiple lines) and feed into my old mono tubs (filter patches 3inch from botto) so the filtered air would blow in straight down from the top and constantly be pushing out the vent filters around the bottom. I could see how this could help but I could also see it blowing potential contams off the tops of caps onto foil. I tend to have a desire to over complicate things lol. What do you think? I have tons of tubs to use I also have plastic shelves I could plastic wrap but I think the tubs will be better


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I’m most things. Less if you don’t ask me. However the things that I’m not make up the rest of me........... mostly

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OnlinePuduwoke
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Jakeoncid419]
    #25939692 - 04/17/19 12:45 PM (8 days, 1 hour ago)

Hi Jake, nice to see you around here!
Since you are here I would be interested in here you take on this also.

So, I know pasteurizing should be 1-2 hours at 140-160F and if you go over 170F for more then about 10 min, it might be a good idea to toss it at start over.
Might be a bit dumb but since I have pros around here like you Bod I would really appreciate you comment on this. I think I found the perfect settings on my stove to keep the temp where I want it, im your curious if you think this is too close to 170F or if maybe you would read is as 170F.

Thank you for your time and help like always :cheers:


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OfflineJakeoncid419
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Puduwoke]
    #25939706 - 04/17/19 12:56 PM (8 days, 51 minutes ago)

Quote:

Puduwoke said:
Hi Jake, nice to see you around here!
Since you are here I would be interested in here you take on this also.

So, I know pasteurizing should be 1-2 hours at 140-160F and if you go over 170F for more then about 10 min, it might be a good idea to toss it at start over.
Might be a bit dumb but since I have pros around here like you Bod I would really appreciate you comment on this. I think I found the perfect settings on my stove to keep the temp where I want it, im your curious if you think this is too close to 170F or if maybe you would read is as 170F.

Thank you for your time and help like always :cheers:




Check it with a thermometer see what temp it holds


--------------------
I’m most things. Less if you don’t ask me. However the things that I’m not make up the rest of me........... mostly

JOC Pan TEK


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OnlinePuduwoke
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Jakeoncid419]
    #25939737 - 04/17/19 01:17 PM (8 days, 30 minutes ago)

Isn't that what I'm using now?

Only have 1 more and I know for a fact that one is not correct so guess I will borrow from a friend and test it next to this one, but right now I was asking for what you guys would say its currently reading and/or if it should be fine?


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OfflineJakeoncid419
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Puduwoke]
    #25939743 - 04/17/19 01:19 PM (8 days, 28 minutes ago)

Yeah lol sry I meant to say test core of bulk (test run) sry


--------------------
I’m most things. Less if you don’t ask me. However the things that I’m not make up the rest of me........... mostly

JOC Pan TEK


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Jakeoncid419]
    #25939749 - 04/17/19 01:21 PM (8 days, 27 minutes ago)

Yeah guess I will just have to do that. If the temp say 170F for more then 1 hour it should almost for sure contam right? Or im I wrong about that?
Thanks


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Puduwoke]
    #25939758 - 04/17/19 01:25 PM (8 days, 22 minutes ago)

No sure to contam but helpful microbes start to die off at 170.  Now most people drop their bulk and 285° water or hotter and the bulk lowers the temperature to around 170 and they hold an approximate temperature for an hour or so most people use coco Coir So it’s not that big of a deal and if some of the microbes are killed on the outer layers the microbes on the inner portion that didn’t get as hot will re-planted shit but then takes longer for your micro of the count to build I am a strong believer in large microbe counts in sub to fight contams


--------------------
I’m most things. Less if you don’t ask me. However the things that I’m not make up the rest of me........... mostly

JOC Pan TEK


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Jakeoncid419]
    #25939802 - 04/17/19 01:50 PM (7 days, 23 hours ago)

alright thanks, yes I remember reading you talking about letting substrate sit for up to 1 week for that reason


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Offlinenewtomyc
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: Puduwoke]
    #25941033 - 04/18/19 08:24 AM (7 days, 5 hours ago)

Hey Bod,

I'm about to buy a microscope. I found a moderatly priced on Amazon (omax compound microscope/ magnification up to 2000).

Do you think that is a ok starter or should I save and spend more?

Was curious of your first one. And what one is looking for in a microscope (besides magnification)


Edited by newtomyc (04/18/19 08:30 AM)


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InvisiblebodhisattaM
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: newtomyc]
    #25941151 - 04/18/19 10:33 AM (7 days, 3 hours ago)

2000x isn't truely 2000x. 1000x is pretty well the limit of optical magnification. 2000x doesn't increase the resolution at all it literally just makes 1000x look bigger.

My amscope was like 120-140 or something like that. The next step up in quality you're looking at a couple thousand dollars. The only thing more $ will buy you until you get to that next price point is gimmicks and less plastic components. But scopes don't get abused so who cares.


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: bodhisatta]
    #25941177 - 04/18/19 10:46 AM (7 days, 3 hours ago)

Thanks!!


--------------------
JJ

Draw unto others as they have been drawn to you.... WSP


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: newtomyc]
    #25947119 - 04/21/19 11:07 AM (4 days, 2 hours ago)

hey bod! so, I have followed your easy AF guides up to this point (other than using hard red wheat berries instead of oats) and this is my 3rd set of shoeboxes. These are growing short round fruits and I can't figure out why, I've read things on the forums like it may be too much humidity or not enough or too much light or not enough or even just bum genetics. What would you do to fix this? I have 5-6 more jars of colonized grain with these genetics so I'd like to see if I can save it through environmental tweaks.
Right now I am using your S3 tek, leaving the lid on (not fully pushed down) during colonization, and then dubtubbing once pins appear. They get gentle natural light from above and I mist 2-3 times a day, keeping the surface wet (the mycelium does look more hardened than it did at first, so maybe it's a moisture problem). I am not getting any side pins.

What would you do?







--------------------
¸.·´¯`·.´¯`·.¸¸.·´¯`·.¸><(((º>


Edited by sunset (04/21/19 11:08 AM)


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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: sunset]
    #25947121 - 04/21/19 11:08 AM (4 days, 2 hours ago)

Those pins look funny like bacterial spawn or using a penis envy type variety


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OfflinePixies
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Coir: 2nd attempt better but contamination? [Re: bodhisatta]
    #25952167 - 04/24/19 06:23 AM (1 day, 7 hours ago)

Hi Bod,

Initially things went much better with second attempt with saf coir method - solid white surface within 6 days but on 7th day strange pinky (later brownish) discolouration appeared in areas of tomentose growth. Contam? Parallel tub using same spawn with hpoo looks fine.



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Re: Coir: 2nd attempt better but contamination? [Re: Pixies]
    #25954135 - 04/25/19 08:20 AM (5 hours, 27 minutes ago)




So first pic is a tub with exposed grains and second I put 1/4 coir layer on.

Question: does my first one's Surface conditions look off? It's starting to pin but not like the other. Spawned same day with same set of plates from MS master plate.


--------------------
JJ

Draw unto others as they have been drawn to you.... WSP


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