[Agar] Contamination or tomentose mycelium? - Mushroom Cultivation

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FiatFirmamentum
Stranger

Registered: 04/15/19
Posts: 57
Loc: Central EU

[Agar] Contamination or tomentose mycelium?

#25935889 - 04/15/19 01:07 PM (1 year, 10 months ago)

MEYA, 4% nutrient. Inoculated 15 days ago, first visible growth around 6 days ago. Incubated in 22*C.

Color: white as white paper. Holes in growth are on inoculation line. Note: media was probably caramelized due too long autoclaving.

MEYA, 2% nutrient with chloramphenicol. Inoculated 7 days ago. Incubated in 31*C.

Color: white as white paper in the middle, a bit yellowish on outside (though, it's translucent).

MEYA, 2% nutrient with chloramphenicol. Inoculated 7 days ago. Incubated in 31*C.

Color: white as white paper in the middle, a bit grayish on outside (though, it's translucent, it can be agar).

All plates were inoculated using sterile (medical one) swab from spore print.

Most of control plates (poured, not opened since) show no sign of contamination (1/10 was contaminated with whitish mold). Some of chloramphenicol plates (not shown here) were exposed to fresh air to deliberately contaminate them to check if chloramphenicol is working - only molds were found on them.

Species: P. cubensis.

Post Extras:

Shroomspective
Looking into the void

Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away

Re: [Agar] Contamination or tomentose mycelium?

[Re: FiatFirmamentum]
#25936097 - 04/15/19 02:46 PM (1 year, 10 months ago)

Top plate looks like germination mycelium, it takes 2-3 transfers to get back to looking normal, middle plate might be suspect, and bottom plate needs a few more days

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