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OfflineNassCorda
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Agar noob - are these contams? Please help!
    #25934289 - 04/14/19 01:45 PM (5 years, 9 months ago)

Hi shroomery! I'm a long time lurker but this is my first post.

I've been trying my hand at agar as I have trouble getting spores/syringes at the moment. This is my very first go at agar and so far I've made a few plates that were inoculated with MS syringes. Of these plates only a few (I think) turned out clean, most of the others were contamed with staph. aureus (possibly, as they were contamed by bright golden/yellow spots), so I made a transfer to new plates saving as much mycelium as possible.

These plates have now grown out a little bit and the "mycelium" has colonized all of the agar that I cut out and is appearing to start to spread outward a bit. This mycelium however looks fuzzy to me and I'd like to submit some pics for you guys to see so I can make sure I didn't accidentally isolate a lot of mold and that it is in fact mycelium from many strains growing at once.

The fluffiest ones were transfered from the bacterial plates from my first try. I will try to label them as best as I can and circle the parts that I think are contams.

Can you pros please help me out figuring out which parts are mycelium and which are contams?

Thank you!


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InvisibleMateja
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Re: Agar noob - are these contams? Please help! [Re: NassCorda]
    #25934363 - 04/14/19 02:28 PM (5 years, 9 months ago)

I would use plates 6,8 and 9 :thumbup:
Make tiny transfers the size of a rice grain. Gl


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OfflineNassCorda
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Re: Agar noob - are these contams? Please help! [Re: Mateja]
    #25934713 - 04/14/19 05:10 PM (5 years, 9 months ago)

Quote:


Make tiny transfers the size of a rice grain. Gl





So basically when I transferred the biggest possible slice of clean agar is where I fd up? Transfers should be tiny?

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InvisibleMateja
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Re: Agar noob - are these contams? Please help! [Re: NassCorda]
    #25934769 - 04/14/19 05:38 PM (5 years, 9 months ago)

The first link in my sig called 'getting started' is a good place to start.
Now I'm wondering if you've even done nearly enough reading on the subject before
attempting to do it becsue the first basic thing you learn when reading about cultivating agar cultures is that the transfer piece should be very small exactly
to promote clean cultures and not anything else hitching along.


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OfflineNassCorda
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Re: Agar noob - are these contams? Please help! [Re: Mateja]
    #25934783 - 04/14/19 05:46 PM (5 years, 9 months ago)

I read plenty but wasn't trying to isolate anything, I just wanted to see if I could get rid of the bacterial contams on my very first plates and save as many substrains of "amazon" as I could.

But hey, learning is half the fun. So thanks :smile:

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InvisibleMateja
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Re: Agar noob - are these contams? Please help! [Re: NassCorda]
    #25934804 - 04/14/19 05:58 PM (5 years, 9 months ago)

Yes the goal is to 'isolate' clean myc away from contamination :super:
Hey at least you're learning and that the importantest :takingnotes:


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