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Xerbia
Stranger

Registered: 02/24/10
Posts: 544
Last seen: 1 month, 19 days
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How do you clean up wild spore prints on agar?
#25933319 - 04/13/19 10:39 PM (5 years, 9 months ago) |
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So I recently received some prints that I’ve been trying to work with on agar. They are ps. Ovoids, ps. Azure’s, ps. Alleni, and I also use some prints from an old grow that the fruits had vermiculite, and another print from an earlier generation before the verm hit.
I work in a VERY clean room in front of a hepa filter. I pour plates fine without ever getting any contams. I did some other cleaner spores as well I printed and all those had no contams. I use a sterile swab to wipe the spores from the print in 3 lines on the agar plate.
I’ve included pics of each plate I’m having issues with. When working with a dirty wild spore print, how the heck can you get a good transfer?? (In the pics the ones labeled perfect spec #2 and big B + were the only non wild, but had troubles with verm during their grows. Does verm carry over through spores as well? The rest are wild prints)
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Geinstein
Shroomery addict



Registered: 01/25/18
Posts: 1,784
Last seen: 3 years, 20 days
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Re: How do you clean up wild spore prints on agar? [Re: Xerbia]
#25933346 - 04/13/19 11:08 PM (5 years, 9 months ago) |
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For me it looks like the contam to spore rate is fucking you You could dilute the spore solution a crap amount
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Nothing breads nothing
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Xerbia
Stranger

Registered: 02/24/10
Posts: 544
Last seen: 1 month, 19 days
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Re: How do you clean up wild spore prints on agar? [Re: Geinstein]
#25933354 - 04/13/19 11:18 PM (5 years, 9 months ago) |
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Quote:
Geinstein said: For me it looks like the contam to spore rate is fucking you You could dilute the spore solution a crap amount
I;m intrigued. How could I go about doing this?
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gamer4life
Natures Child



Registered: 05/29/10
Posts: 810
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Re: How do you clean up wild spore prints on agar? [Re: Xerbia]
#25933380 - 04/13/19 11:55 PM (5 years, 9 months ago) |
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You could also go to market place here at the shroomery and maybe find someone with some cleaner prints. I had recieved some prints and scored with making some nice lc syringes after a little agar work. I mean first try and 8 plates all of them made a perfect nickel size of mycelium. I also bought already poured agar plates from out-grow a shroomery sponsor.
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SFS96
AstroMan



Registered: 12/09/18
Posts: 2,146
Loc: Valleys Of Neptune
Last seen: 7 months, 29 days
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Re: How do you clean up wild spore prints on agar? [Re: Xerbia]
#25933396 - 04/14/19 12:09 AM (5 years, 9 months ago) |
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I don’t think there is mycelium on any of those dishes. I haven’t worked with wild prints much yet but maybe try and not swab so many spores on the plates. The more spores you swab from a wild print I would think the more contams your going to transfer over. These are just my thoughts lol I hope someone more experienced chimes
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: How do you clean up wild spore prints on agar? [Re: SFS96]
#25933664 - 04/14/19 07:20 AM (5 years, 9 months ago) |
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I've had a very easy time isolating from wild prints, I would source other prints and try again.
I think I have a load of ovoid prints from 2016 somewhere, if only I knew where you'd be getting one.
It is the season for them though so you may be in luck.
Consider using a sab if you think the hood is blowing contams, as a final thought.
Good luck.
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: How do you clean up wild spore prints on agar? [Re: Xerbia]
#25933724 - 04/14/19 08:19 AM (5 years, 9 months ago) |
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Quote:
Xerbia said:
Quote:
Geinstein said: For me it looks like the contam to spore rate is fucking you You could dilute the spore solution a crap amount
I;m intrigued. How could I go about doing this?
With water
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Alchemycologist
1 in 10,000

Registered: 04/09/19
Posts: 285
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Re: How do you clean up wild spore prints on agar? [Re: bodhisatta]
#25933784 - 04/14/19 09:07 AM (5 years, 9 months ago) |
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Using a swab on wild prints is asking for trouble. That's way too many spores on one spot. Use an inoculation loop and dilute, as said above.
-------------------- Yep.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: How do you clean up wild spore prints on agar? [Re: Alchemycologist]
#25933828 - 04/14/19 09:33 AM (5 years, 9 months ago) |
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Its interesting every dish has the same aspergillus mold. Makes me think its operator more than prints. If the prints were that bad it would be a myriad of different contaminants. One dish has bacteria(or maybe yeast), the alleni one. I just think it would be extremely rare every print is loaded with the same mold.
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Geinstein
Shroomery addict



Registered: 01/25/18
Posts: 1,784
Last seen: 3 years, 20 days
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Re: How do you clean up wild spore prints on agar? [Re: bodhisatta]
#25934002 - 04/14/19 11:04 AM (5 years, 9 months ago) |
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Quote:
bodhisatta said:
Quote:
Xerbia said:
Quote:
Geinstein said: For me it looks like the contam to spore rate is fucking you You could dilute the spore solution a crap amount
I;m intrigued. How could I go about doing this?
With water
Lol
Quote:
bodhisatta said: Its interesting every dish has the same aspergillus mold. Makes me think its operator more than prints. If the prints were that bad it would be a myriad of different contaminants. One dish has bacteria(or maybe yeast), the alleni one. I just think it would be extremely rare every print is loaded with the same mold.
Was thinking the same but didn't want to say
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Nothing breads nothing
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Xerbia
Stranger

Registered: 02/24/10
Posts: 544
Last seen: 1 month, 19 days
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Re: How do you clean up wild spore prints on agar? [Re: filthyknees]
#25934080 - 04/14/19 11:53 AM (5 years, 9 months ago) |
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Quote:
filthyknees said: I've had a very easy time isolating from wild prints, I would source other prints and try again.
I think I have a load of ovoid prints from 2016 somewhere, if only I knew where you'd be getting one.
It is the season for them though so you may be in luck.
Consider using a sab if you think the hood is blowing contams, as a final thought.
Good luck.
The hood is fine, i've not had contams on any other plates, and they only came up where I swabbed. I may try a different agar recipe to to make the myc more vigorous.
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Xerbia
Stranger

Registered: 02/24/10
Posts: 544
Last seen: 1 month, 19 days
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Re: How do you clean up wild spore prints on agar? [Re: bodhisatta]
#25934083 - 04/14/19 11:55 AM (5 years, 9 months ago) |
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Quote:
bodhisatta said: Its interesting every dish has the same aspergillus mold. Makes me think its operator more than prints. If the prints were that bad it would be a myriad of different contaminants. One dish has bacteria(or maybe yeast), the alleni one. I just think it would be extremely rare every print is loaded with the same mold.
Well they did all come from the same person so who knows. I've not had any contam issues with other plates at all.
I talked with who I got them from and he gave a good recommendation I'll be trying.
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