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OfflineJericho_Button
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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26312872 - 11/11/19 01:37 AM (4 years, 6 months ago)

I have some Pan jars that are fully colonised, maybe a bit too much. I had to go away for 10 days and when I got back some of my jars had maxed out. It's been a week since then. I wanted to store some jars in the refrigerator for a few weeks but I had read not long ago that Pan Cyan mycelium loses vitality when subjected to cold temperatures. What would you recommend? I have just pasteurized my first batch of manure (2 days ago), but I may spawn early as I'm eager to see if my culture will fruit, and at this stage aiming for 3 flushes seems unnecessary. I also need the space.

As for the casing, do you pasteurize and leave to recuperate like the substrate? Or do you case immediately after pasteurization?

Cheers for any advice.


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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: Jericho_Button] * 2
    #26313221 - 11/11/19 08:34 AM (4 years, 6 months ago)



(Pasteurization time 4 hours).

According to that Image, you can see how population of good microbes builds up over time after the pasteurization has been done, days after. Also it seems that pasteurizing at a constant 150F kills less beneficial microbes population vs 160F.

150F ended up with around 250,000 more population compared to 160F after the same recovery time.  150F needs half the time to reach to the same level of population that 160F reached at the end of the row.

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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Jericho_Button] * 1
    #26314802 - 11/11/19 10:29 PM (4 years, 6 months ago)

Quote:

Jericho_Button said:
I have some Pan jars that are fully colonised, maybe a bit too much. I had to go away for 10 days and when I got back some of my jars had maxed out. It's been a week since then. I wanted to store some jars in the refrigerator for a few weeks but I had read not long ago that Pan Cyan mycelium loses vitality when subjected to cold temperatures. What would you recommend? I have just pasteurized my first batch of manure (2 days ago), but I may spawn early as I'm eager to see if my culture will fruit, and at this stage aiming for 3 flushes seems unnecessary. I also need the space.

As for the casing, do you pasteurize and leave to recuperate like the substrate? Or do you case immediately after pasteurization?

Cheers for any advice.




So I had a pan Alabama jar that was ready about 2 weeks before I gotta see it. It’s the tray I just posed in the pan thread. It’s a iso that has always given me canopies but this time I just got some sparse fruits. The only thing I can think of is the jars age. I’d still run it tho.
As for the case I also let the microbe count build after pasteurization


--------------------
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OfflineJericho_Button
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26315023 - 11/12/19 02:13 AM (4 years, 6 months ago)

Ok, it still looks pretty good, tons of growth, but also a few metabolite secretions on a couple of jars. I had 10, of which 3 grew really slowly. I shook these ones prior to going away, and returned to them being the only fully colonised. I shook the rest, 3 of which have also fully colonised, but 4 haven't really recovered. It's not a complete stall as I see some fresh mycelium being produced, but I'm guessing it's a poor culture. I'm not entirely sure I had managed to get the ideal phenotype, it was still somewhat tomentose before going to grain, but after 4 transfers I thought I may as well go ahead and give it a shot! At this point, a single dose for all my efforts would be satisfaction enough. I'm hoping if I get anything, performing a clone will set me towards getting a culture that will produce a yield similar to what you seem to achieve.

Thanks to you & Mr. Alien for the advice. I will keep patient, I'm just really worried about spoiling everything by leaving my jars too long. I reckon by the time I get them spawned half will be nearly 3 weeks since full colonisation, and half around 2 weeks, so I guess there's still a good chance of at least getting some fruits. I have another plate on the go with some fairly good looking Pan mycelium, so I can always set up another batch of jars, but I will have to wait to spawn these ones, as the rest of my jars are currently occupied by Treasure Coast mycelium haha. Thought I'd try and do everything all at once.

I've nearly got all my gear for the martha too, just waiting on my drip tray and my timers.... I'll post pics of the set up.


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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26318733 - 11/13/19 09:44 PM (4 years, 6 months ago)

Have you tried this with millet?

https://www.shroomery.org/forums/showflat.php/Number/23322748/fpart/3/vc/1#23322748

No soak/no simmer sounds soooo convenient.

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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26318760 - 11/13/19 09:59 PM (4 years, 6 months ago)


I ran my wbs and millet like that off and on for years and if I'm lazy I still do.
It works but when they get starchy it slows shit down.
For consistency I prefer a hands on prep, perfect grain can be a huge key to good colonization IMO
I used volume, cup and a half of millet and 3/4 cup of water .
Toss in the pc and shake while hot


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Re: JOC TEK Panaeolus cyanescens [Re: cronicr]
    #26318774 - 11/13/19 10:09 PM (4 years, 6 months ago)

:bow2: this tek....
:pantytail:


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Tek's I use

LAGM2020

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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: cronicr]
    #26318803 - 11/13/19 10:31 PM (4 years, 6 months ago)

Quote:

cronicr said:

I ran my wbs and millet like that off and on for years and if I'm lazy I still do.
It works but when they get starchy it slows shit down.
For consistency I prefer a hands on prep, perfect grain can be a huge key to good colonization IMO
I used volume, cup and a half of millet and 3/4 cup of water .
Toss in the pc and shake while hot




Nice! I think i will prepare it properly then. Most guys just soak 24hr and load the PC, any benefit in simmering after the 24 hr soak with millet?

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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26318813 - 11/13/19 10:36 PM (4 years, 6 months ago)

No I just start with scolding water


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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: cronicr]
    #26318850 - 11/13/19 11:15 PM (4 years, 6 months ago)

Quote:

cronicr said:
No I just start with scolding water




Sorry for my ignorance but what does "scolding water" means in English?

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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26318853 - 11/13/19 11:16 PM (4 years, 6 months ago)

I start by pouring boiling water on it( I use an old pc or a bucket)


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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: cronicr]
    #26318859 - 11/13/19 11:20 PM (4 years, 6 months ago)

Quote:

cronicr said:
I start by pouring boiling water on it( I use an old pc or a bucket)




Got ya! thanks.

Jake, Once spawn reaches 100% colonization, do you shake to see if it recovers? or nah.

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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26318865 - 11/13/19 11:24 PM (4 years, 6 months ago)

Not at 100 I usually do a shake at about 90% then bulk it once it bounces back


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26319364 - 11/14/19 08:47 AM (4 years, 6 months ago)

Quote:

Jakeoncid419 said:
Not at 100 I usually do a shake at about 90% then bulk it once it bounces back




But what if you shake at 90% and it bounces back but not fully at 100% but at 99%? I would be very scare to shake at 90% and bulk at 99%.

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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26319445 - 11/14/19 09:25 AM (4 years, 6 months ago)

That doesn’t ever really happen for me normally if the jar is going to stall it does it at about 40% a good culture will eat a jar in seven days 10 days being the most much beyond that and I consider the culture “not worth it”


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26319456 - 11/14/19 09:31 AM (4 years, 6 months ago)

Quote:

Jakeoncid419 said:
That doesn’t ever really happen for me normally if the jar is going to stall it does it at about 40% a good culture will eat a jar in seven days 10 days being the most much beyond that and I consider the culture “not worth it”




I see, thanks a lot jake! :grin:

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OfflineJericho_Button
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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26337413 - 11/22/19 04:26 AM (4 years, 5 months ago)

Hey man, got my trays ready to case, and my Jartha is set up and ready to go! Gonna give my trays another 2 days to fully colonise before casing, then I was thinking 24 hours to consolidate after casing, what do you think? And is it important that the casing is at 'field capacity' during that 24 hour consolidation period? Or can I just mist when I put them out to fruit?

Take a look and let me know what you think, it's not the best looking culture, and the jars were a little slow and were also way overdone when I finally spawned, but it took 3/4 days to get to what looked like 90% substrate colonisation so I'm anticipating at least a couple of fruits!



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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Jericho_Button]
    #26337748 - 11/22/19 09:19 AM (4 years, 5 months ago)

Looks good to me! Keep ur case paper thin. I only give them 12 hours back in incubation after applying casing. I usually case at night & place in FC in the morning. The case doesn’t have to be super wet durning 12 hour incubation period. But mist it down good when u first put in FC, then just keep a close eye on it while you dial in ur timers. I’m excited to see how they turn out bud keep me posted!!!


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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InvisibleMr. Alien
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Re: JOC TEK Panaeolus cyanescens [Re: gtshroom]
    #26340446 - 11/23/19 02:39 PM (4 years, 5 months ago)

Quote:

65% chopped straw (no need to pre hydrate)
35% manure
5% verm
1/2 cup gypsum (optional)
Mix in water and bring to field capacity




so 105%?  :wow: Is not 100% based? this is volume right not weight.

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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Mr. Alien]
    #26340557 - 11/23/19 03:39 PM (4 years, 5 months ago)

Lol sry supposed to be 30% manure. I don’t measure exactly tho I just approximate that ratio in a bucket


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

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