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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: LongJonSilver]
    #26835063 - 07/20/20 09:06 PM (3 years, 9 months ago)

2 hours


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26835082 - 07/20/20 09:11 PM (3 years, 9 months ago)

Oh so I posted this in the official pan thread But I should probably put it in here also
but I should probably put it in here also
So I had been having a lot of trouble with my bulk contaminating after I add my spawn as spring started. now at first I thought it was my manure quality and part of it was as the stuff I was using for a little while there was total garbage but now I have my optimal Pooh and I was still having problems with my pasteurized trays almost all of them were contaminating I had changed nothing about my process the only difference is the season so for the last month and a half or so I’ve been sterilizing my bulk and Myco bags then inoculating with grain. It’s Working really well so far the reason I normally stuck with pasteurization was because in my experience those trays just seemed to resist contamination longer so far these trays are holding up about the same amount of time 2.5 flushes average. I would imagine it has something to do with The summer air and it finding its way in through small cracks in the trays while they’re colonizing (pasteurized tek). So yeah if you find yourself running into contamination after contamination switching over to the sterilized  bags is a good way to isolate your problem.


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OnlineA.k.aM
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26835148 - 07/20/20 09:56 PM (3 years, 9 months ago)

How long were they taking to contaminate?

I sterilized all my subs before but just recently started pasteurization because they were blowing up with mold while the second flush was still pins.

So far all six pasteurized ones have either hit 100% colonization or will in a day so I’m hoping they do ok. The only thing that’s got me worried is the last tub I did a month ago colonized great but then never pinned and went green 5-6 days into fruiting. The spawn was iffy though.


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: A.k.a]
    #26835157 - 07/20/20 10:00 PM (3 years, 9 months ago)

See that’s the only advantage to the pasteurized sub on average pasteurized trays made it to three flushes Whereas the sterilize trays average 1.5, So far I’ve had pretty good results in terms of how far in this sterile sub was making it I’m actually starting to think that it’s more likely an issue with my electric cookers they have been getting worked pretty hard I should see if I can’t test the temperature


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26835164 - 07/20/20 10:03 PM (3 years, 9 months ago)

But you Gotta keep your tent clean you got To clean your humidifier every few weeks I try and wipe the inside of the tent down as often as I can and replace the plastic every few months all of these things will extend the life of your trays but the beauty of pans is how fast they move even if you only get one good flush per tray that’s still more than enough whether you’re looking for prints or fruits, And because of how fast they move you can just line them up to replace each other one after the next


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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26835189 - 07/20/20 10:22 PM (3 years, 9 months ago)

I noticed with the tropicalis I had on agar looked like crap no matter how many different germ plates I did and transfers I tried. Don’t know if it was genetics or just how tropicalis var. is now. I noticed though that it does a whole lot better in Liquid culture and on grain. It’s like it just doesn’t get what it needs out of the agar at all.


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Celestialexplorer1]
    #26835205 - 07/20/20 10:51 PM (3 years, 9 months ago)

Yeah the tropicalis that goes around is a very finicky strain most of my attempts and in failures I think I’ve only ever gotten three or four trays of it to come up ever however the pan Australia is tropicalis according to its microscopic features (still no PCR results yet but I’m hoping it won’t be too much longer we’re just waiting on getting enough samples together so that we get a discount on the sequencing we need a total of 96) But the pan Australia is very aggressive and not at all like the tropicalis labeled under tropicalis that circulates the vendor sites. That one is very picky perhaps the most picky pan I’ve worked with yet well pan Africa may soon be taking its place because I still don’t have any pens on those trays and they looked perfect so I’m pretty confused at this point


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OfflineCelestialexplorer1
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26835246 - 07/20/20 11:27 PM (3 years, 9 months ago)

Last time I tried tropicalis I made 4 trays that sat for two weeks until the begging got of summer and then the gnats came and made a big fuck tent right in one of the trays so I ended up chucking everything. One of the trays though had this white mass on it that just grew and grew until it was about golf ball size and when I plucked it off and went to clone it I did a mid section and the whole thing turned dark dark blue. I really wish I had taken pictures it was kinda crazy. I believe the clone was too badly contamed. Hopefully have better luck
This time. Got my fogger coming Wednesday.


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Edited by Celestialexplorer1 (07/20/20 11:34 PM)

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OfflineAlphaDog52
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Re: JOC TEK Panaeolus cyanescens [Re: Celestialexplorer1]
    #26840172 - 07/23/20 09:54 AM (3 years, 9 months ago)



Would this type of filter be suitable to install at the top?

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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: AlphaDog52]
    #26840368 - 07/23/20 11:08 AM (3 years, 9 months ago)

Yep that would work just fine you do not have to use a hepa


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OfflineCelestialexplorer1
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26840447 - 07/23/20 11:44 AM (3 years, 9 months ago)

So watching the tent after I set it up I was able to get it 78 in there by placing a heater close to the filter and the rh fluctuates between 75% near the end and after the fan run during evap and then goes back up to about 95% until the fan starts again. That seems like a pretty big dip and I know that hydro meters are trash don’t use them and all that but I wanted to see lol also put a humidifier on the lowest setting on the bottom to stabilize humidity till I get a bigger fogger. Without that there I would be a lot lower in the rh I’m sure. Do you know what ranges your tent is in


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Edited by Celestialexplorer1 (07/23/20 11:45 AM)

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OfflineLongJonSilver
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Re: JOC TEK Panaeolus cyanescens [Re: Celestialexplorer1]
    #26840853 - 07/23/20 03:00 PM (3 years, 9 months ago)

What about the fuck tent?!! The gnat pirates were cleaning the mycelium barnacles off your cakes in prep for hunting down the Urca De Lima. (Black Sails)

I noticed the same thing with the tropicalis. I did a bunch of agar plates and most of them all looked weird. The first 3 or four of the bisporous and the Australia ones all looked way better. I mean I dont really know that they are better cause this is my first time trying any pans but they looked more uniform I guess.

Also I just ordered some dehydrated horse manure. I think the manure I was using was part of the problem also. I noticed when I used it for cubes it took a long time to colonize. I sifted a bunch of it and it was more than half wood chips. I'm going to try using the sifted stuff while I am waiting on the horse manure.

I think improving the straw length and manure quality will help things on my next try.

I also tried just taking the covers off two of those trays that werent colonizing to see if it might help. It looks like the mycelium is covering a little more of the cake but not much.

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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26840880 - 07/23/20 03:19 PM (3 years, 9 months ago)

Here are some of the agar plates I have going right now. Do any of them look more promising than the others? I am about to put 7 of the Pan Bis. and Pan As into jars tomorrow morning when the PC is cooled.

Im going to try every one prob but rather do the promising looking ones first if I can. By the way how long will these agar plates last?





Edited by LongJonSilver (07/23/20 03:26 PM)

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OfflineCelestialexplorer1
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Re: JOC TEK Panaeolus cyanescens [Re: LongJonSilver]
    #26840909 - 07/23/20 03:39 PM (3 years, 9 months ago)

What kind of nutrition are you using for those plates there? On some of those plates the myc looks like it’s starved for something when it gets hard like that. Some look like they might be alright but I can’t see them too well. I never could trust the ketchup cups though but it seems to work good enough for some.

Also
I have a question with the joc tent do you do anything different when the pins start to show like change the timing or dial it different at all? Only ask cuz once the pins start I would feel like you wouldn’t need evap as much.


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Edited by Celestialexplorer1 (07/23/20 03:44 PM)

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Re: JOC TEK Panaeolus cyanescens [Re: Celestialexplorer1]
    #26841473 - 07/23/20 08:15 PM (3 years, 9 months ago)

I am using a pre made mea mix for the agar. I made it with a little less of the mix than called for this time cause I thought maybe less nutes would make it spread out more looking for more nutes. Didnt seem to really work though. I was wondering if that is normal or not the way they are doing that. You think I should add more of the mix to the water next time?

I also have some on potato yeast agar but they look pretty similar. The ketchup cups seem to work ok for me I have like ten of the real agar plates but they seemed pretty expensive and I wanted to do quite a few to experiment with. I have only had a couple with any contams. I think I got a pack of 100 for like 5 or 10 dollars.

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Re: JOC TEK Panaeolus cyanescens [Re: LongJonSilver]
    #26841517 - 07/23/20 08:39 PM (3 years, 9 months ago)

If you drop the nutes too low you can slow down the culture but it could also be your genetics I like peptone but I’ve used other new profiles and most of them work most of the time but peptone usually works the best for me


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Edited by Jakeoncid419 (07/23/20 08:40 PM)

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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26841548 - 07/23/20 09:00 PM (3 years, 9 months ago)

I was kind of thinking it was more the genetics cause of the Pan Trops but Celestial brought up the ketchup cups and I was wondering if the cups were letting more air in than the plates would and if that could change how the culture spreads if it dried out a little or something. IDK but they seem to be doing ok. I just gotta get one for each thats decent out of all of them then I can transfer from the plate that ended up being good and take some clones. I remember what you said about safety in numbers.

The Pan Trops were originally no good cause I think they were over heated in transit or something but I was able to get a small quarter size of mycelium on one jar out like 10 or more and used that little bit to put one rye berry onto agar. It wasnt ideal but I wanted to keep doing something while the other prints were on the way. I thought maybe once I got the new pan B and Pan C prints they wouldnt do that anymore and they didnt at first but looks like they are now.

I was putting off getting peptone just because the price was pretty high but maybe I will if it will change the results. For now I will just use what I got and hopefully get at least one good tray of each.

I also have one thing of liquid culture I made but I have no idea if its made from a good sample I just used grain water and was already sterilizing some q-tips so figured I would try it.

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Re: JOC TEK Panaeolus cyanescens [Re: LongJonSilver]
    #26841646 - 07/23/20 10:08 PM (3 years, 9 months ago)

What model electric rice cooker/pressure cooker holds a perfect 162F?

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Re: JOC TEK Panaeolus cyanescens [Re: Hartford]
    #26841709 - 07/23/20 10:43 PM (3 years, 9 months ago)

The me I have is a fagor electric cooker


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Re: JOC TEK Panaeolus cyanescens [Re: Hartford]
    #26841714 - 07/23/20 10:45 PM (3 years, 9 months ago)

Everytime I’ve put pan trop spores to agar they never look right. There never white more of a gray. But the cultures in that picture the ones that are white and lumpy almost look like they are cracked or something I’ve seen this same thing using agar plates bought online and the online thing I could possibly pin it to was not enough nutes in the mix maybe. Or I don’t know maybe too much. I actually don’t know I just know that other plates I was using at the same time with the same cultures were doing fine and with those plates this happened




First two pics are the bad plates and last one is a transfer from the bad plates to a good one. You can clearly see something is wrong there.

Edited by Celestialexplorer1 (07/23/20 10:48 PM)

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