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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Legend21]
    #26117840 - 07/19/19 02:10 PM (1 year, 4 months ago)

I cut it up as fine as I can but that will work also the more you cut it up with the easier a time you’re going to have maintaining consistent moisture. I don’t bother pre-hydrating my straw I mix it in with everything else and then bring all of it to field capacity at once


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26117846 - 07/19/19 02:13 PM (1 year, 4 months ago)

Yeah that’s what I tried doing with this is bringing it all to field capacity at once. I was going to cut it smaller but thought somewhere along the line I read 1-2 inches :shrug:



So in the future if I used a blender that wouldn’t be too fine for the straw? I appreciate you always helping man!


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Legend21]
    #26117910 - 07/19/19 02:51 PM (1 year, 4 months ago)

Yup blender is fine, I actually found these bales of fine chopped straw at the local feed store they are $25 a bale but a bale lasts a good while


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26117954 - 07/19/19 03:17 PM (1 year, 4 months ago)

Awesome so it doesn’t matter how fine it is. Thanks man next time I’ll do the straw in smaller chunks. Probably could add more straw this go around since I didn’t pack it in when I measured.


Do you usually add a quart of spawn plus your four quarts of substrate all into one tray?


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Re: JOC TEK Panaeolus cyanescens [Re: Legend21]
    #26117971 - 07/19/19 03:27 PM (1 year, 4 months ago)

Yeah it’s about that I don’t measure my bulk in the jars I make 2 1/2 gallon Ziploc bags and eyeball it by hand as I fill the tray but yes 1 quart jar to a tray and the ratio looks about 1:4. Keep ur sub under 3”. The thicker the sub (max out 3.25) the more flushes you will get it however it is also more difficult to get full pin sets you really gotta crank the cycling full blast and unless everything is on the sane time table (which it never is) this can cause some problems. if you go thin (around 2 inches) it’s easier to get a good flush but you usually only get one


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26135474 - 08/14/19 02:26 PM (1 year, 3 months ago)

Quote:

Jakeoncid419 said:




Anyway here is the recipe:
  • 65% chopped straw (no need to pre hydrate)
  • 35% manure
  • 5% verm
  • 1/2 cup gypsum (optional)
  • Mix in water and bring to field capacity







Are these volumes by weight or?


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Legend21]
    #26135531 - 08/14/19 02:52 PM (1 year, 3 months ago)

They Don’t have to be exact just an approximation


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
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[


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InvisibleChanga Alchemist
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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26135543 - 08/14/19 03:03 PM (1 year, 3 months ago)

Are you still using fulvic acid instead of lime?


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Offline1WithU
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Re: JOC TEK Panaeolus cyanescens [Re: Changa Alchemist]
    #26135689 - 08/14/19 04:10 PM (1 year, 3 months ago)

:threadmonitor:


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: Changa Alchemist]
    #26135740 - 08/14/19 04:34 PM (1 year, 3 months ago)

Quote:

Changa Alchemist said:
Are you still using fulvic acid instead of lime?



No not for the pans, I noticed to difference (except cost) just as long as ur ph is 7-7.5


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26144873 - 08/20/19 05:16 PM (1 year, 3 months ago)

Amazing write up!!


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Re: JOC TEK Panaeolus cyanescens [Re: newtomyc]
    #26145510 - 08/21/19 12:32 AM (1 year, 3 months ago)

Well I've been saying I was gonna make one of these for a while and I finally did. But I forgot the damn Velcro lmao

About to go get some Velcro and then dial this baby in. :biggrin:


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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26148965 - 08/23/19 06:59 AM (1 year, 3 months ago)

Hi man, I hope you don't mind but I have a few questions regarding your set-up:

Quote:

Jakeoncid419 said:

The next day I take it out and set on shelf in Lab for the microbe count to Build.






Do you let the substrate sit in sealed ziplock bags? Or can it be sat in unsealed bags? I would imagine sealed bags with no filter will cause the substrate to go anaerobic no? Or do unsealed bags allow contaminants to take hold?

Quote:

Jakeoncid419 said:

Then the fogger kicks off and the fan continues to run for 45 seconds  to 1min (I'm constantly adjusting times slightly as the room rh changes)






I'm going to be setting up in a small empty bedroom, I've been concerned about the potential of moisture buildup in the room, so I will be using a dehumidifier outside of the automated FC setup, I was thinking that maybe this could prevent you from having to monitor and adjust the automation to suit the RH of the room, seeing as a dehumidifier, in theory, should maintain a constant RH regardless of ambient condition of the weather etc. What do you think to this?


Ragarding your Pano Tub TEK, I'm a little confused by the description, and seeing as the images supplied show a variety of setups, I'm a little worried about getting my setup to a point where I know for certain that it conforms to your specifications. I've drawn a small diagram of what I think the Pano Tub TEK is supposed to look like based on the description and images given (bearing in mind that I will NOT be using an air condenser):


The small arrows pointing from the side of each tub represent the air leaving through the filters above the level of the substrate.

Quote:

Jakeoncid419 said:

I have 2 stacks of 3 tubs on either side of a center tub that has been fitted with a fogger and bathroom fan (blowing in).







Do you not filter the air that is blown into the 'expansion chamber'? I was thinking of finding a way to use a HEPA filter before the fan, but I'm sure I read on this thread that you were having poor yields when using a filter for the Pano Tub system.

Quote:

Jakeoncid419 said:

I have 2 stacks of 3 tubs on either side of a center tub that has been fitted with a fogger and bathroom fan (blowing in).

-
-

This is your air handling system. Off of this center tub are 3 hoses on either side which lead into high side of your tubs. Use a 2.5 gallon water jug and attach it to the humidifier (same model as the jartha). Off of it i have 6 hoses leading into each tub right next to the air line.






So are you saying here that you have BOTH the centre tub and this other 2.5 gallon jug fitted to humidifiers/foggers? Thus the jug is like a second 'expansion chamber'? If this is the case, how do you regulate the final RH in the FCs? Do you aim for a particular RH for the hot air entering the middle tub and then adjust the additional humidity line direct to the FCs by directly monitoring the RH in the FCs?

I have a hygrometer for fogger switching, do you think if I measured the final RH in one FC it could be assumed that it would match the other 5? Thus using the on hygrotherm to trigger the fogger on the humidity humidity lines, maintaining the correct RH across all 6 FCs?

Here is another diagram according to this setup description:


Please take a look and let me know if I'm getting the right or wrong idea.

I've got some cultures going from a print and I'm about to do my first transfers to clean up (this is my second attempt as my first culture was a bit of a mess and I went straight to grain with stalling across all jars, my grain may have also been a bit too wet - rye & millet). Here's some pictures, I'll do my plate transfers in a few days when my new dishes arrive (I pre-pour agar to borosillicate plates before PCing as I won't be working on a big enough scale to justify PCing a large agar batch then pouring to plates - it's convenient and as far as I'm aware, totally secure as I PC the plates inside plastic freezer bags that create a vacuum seal when cooked, so when the plates come out they're sterile and vacuum sealed!).
I wanted to share some pictures of my cultures as the spore print I'm using I had tried to re-hydrate due to it having been stored in a freezer for 3/4 years. I used sterile water but there is still a good chance I caused some contamination in the process, as the first time I cultured these spores to agar the culture looked so perfect, but on these attempts there seems to be some surface wrinkling being caused between the mycelial mat and the upper surface of the agar. I'm sure it's just down to the process of the myceilum feeding on the agar, but I'm still unfamiliar with this and would like some reassurance that the culture would likely be viable after 2 transfers to clean up prior to colonising grain. Anybody feel free to chime in; sorry for the photos too, I had the wrong micro pore tape and had to go OTT with it to ensure the petri dishes were closed securely.






Thanks in advance for any responses! Hope yer all good.


--------------------
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Re: JOC TEK Panaeolus cyanescens [Re: Jericho_Button]
    #26149112 - 08/23/19 10:10 AM (1 year, 3 months ago)

Posting to reserve. Thank you.


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OfflineJakeoncid419
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Re: JOC TEK Panaeolus cyanescens [Re: p9hu7]
    #26149684 - 08/23/19 04:49 PM (1 year, 3 months ago)

yeah I went thru several designs before I found one I liked the best.
the most optimal setup had a bathroom fan pull air in thru a heap into a sterlite container (expansion chamber A) then feeding into the otherside is your fogger equipted with a check valve so the fan doesn't backfeed your fogger. hoses branch off of this chamber and feed into your grow tubs. this is your fae system. I found that with the tubs you still want to slightly hydrate the air or you will fry fruits (this is because the tub is also getting fae thru your cut outs) . then you have another smaller expansion chamber ( I like the 2.5 gal water jugs) this is your rehydration system. its is fed with a fogger then branches off into your grow tubs. you then cycle these to systems just like on the tent set up. I had little rh gauges in the tubs but really you will learn to just look at your sub and see how it needs adjusted.


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26154687 - 08/27/19 04:59 AM (1 year, 3 months ago)

Hey Jake, Just wondering if this pan Cam growth looks good to you mate?



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Re: JOC TEK Panaeolus cyanescens [Re: YarnDi]
    #26160112 - 08/30/19 02:36 AM (1 year, 2 months ago)

Let's see how it goes



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Re: JOC TEK Panaeolus cyanescens [Re: YarnDi]
    #26160719 - 08/30/19 01:57 PM (1 year, 2 months ago)

Yup culture  looks good from here, and the tent looks food also! Excellent work! What size fan you got on there? Looks smaller than mine but I should still work you man just have slightly different cycle times. Don’t forget  you still need to lay out a sheet of plastic under your tent then fold it up and tape it around the sides of the tent. Then I use a Velcro strip for where it goes across the door. You need to make sure most of the air is drawn in they your filters up top.


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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Re: JOC TEK Panaeolus cyanescens [Re: Jakeoncid419]
    #26160787 - 08/30/19 02:59 PM (1 year, 2 months ago)

Thanks Jake, I was thinking that culture was to thick. I was going to chuck it and start again. The fan is 100mm bathroom exhaust fan it works good, when it kicks in I can feel all the air come through the filters,the gh sucks in then when it kicks off it expands kind of like its breathing I guess. But for controlling it I have an inkbird IHC-200 ever used that b4, I brought it before I read this tek?


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Re: JOC TEK Panaeolus cyanescens [Re: YarnDi]
    #26160806 - 08/30/19 03:17 PM (1 year, 2 months ago)

I mean it’s hard to see but no I don’t think it looks to thick. I’ve had good cultures that looked like that. Pan cam myc does grow a lil thicker on average than cyan anyway


--------------------
I’m most things... less if you don’t ask me, however the things that I’m not...
Make up the rest of me...mostly

JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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