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Offlineplanit
natural
Registered: 01/30/04
Posts: 165
Loc: hilly glacier washout
Last seen: 12 years, 10 days
Cloned tissue in corn syrup water
    #2592026 - 04/22/04 07:31 PM (13 years, 4 months ago)

tissue sample was dropped into a half pint jar with rougfly 100ml of water + a teaspoon of corn syrup,problem is cat forgot to put any h2o2 in , MY QUESTION IS , should cat risk contamination and let the jar incubate or should cat risk contam by poking through the filter disk and inject h202 through the needle hole ?


Edited by planit (04/22/04 09:28 PM)


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InvisibleJoshua
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Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Cloned tissue in corn syrup water [Re: planit]
    #2593720 - 04/23/04 05:01 AM (13 years, 4 months ago)

Yes.
:grin:
Joshua


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InvisibleCitric
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Registered: 03/19/04
Posts: 4,207
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Post deleted by Anno [Re: Joshua]
    #2593796 - 04/23/04 05:29 AM (13 years, 4 months ago)



--------------------
Self Healing lid tek
Fast, easy, fool proof inoculation
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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InvisibleJoshua
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Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Cloned tissue in corn syrup water [Re: Citric]
    #2593856 - 04/23/04 06:13 AM (13 years, 4 months ago)

I know, that's why I smiled. My answer wasn't really an answer.

Here's my answer.

If the wedge was sterile and transfered in a sterile manner, then there is no need for the peroxide other that as an extra precaution.

If there is a concern about the sterility of the wedge, then yes some peroxide is likely to help. However, if too much time has elapsed it is possible any contaminant spores may have already germinated. It is best to add the peroxide at or before the time of transfer for best results.

I was just too lazy to type that out before.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


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InvisibleCitric
 User Gallery

Registered: 03/19/04
Posts: 4,207
Trusted Cultivator
Post deleted by Anno [Re: Joshua]
    #2593886 - 04/23/04 07:15 AM (13 years, 4 months ago)



--------------------
Self Healing lid tek
Fast, easy, fool proof inoculation
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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Offlineplanit
natural
Registered: 01/30/04
Posts: 165
Loc: hilly glacier washout
Last seen: 12 years, 10 days
Re: Cloned tissue in corn syrup water [Re: Citric]
    #2595303 - 04/23/04 04:50 PM (13 years, 4 months ago)

cat did this in a glovebox "sterilized" by 99% iso alc prayed from inside the box with a hair spray nossle which produced a fine mist, when cat smells the box with the plexi top slightly open its an intense alchaho smell , ccat doent think much could survive that.oh and thanks again josh,kittys starting an outdoor bed this summer  :grin:


Edited by planit (04/23/04 04:55 PM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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