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OfflineSubfinder
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Registered: 07/01/18
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915496 - 04/04/19 01:21 PM (5 years, 9 months ago)

I'd go spores to agar. Keep what you have going.

I'd skip the LC for now, stick to agar. Cut a wedge when it's cleaned up and drop it to grain.

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915517 - 04/04/19 01:41 PM (5 years, 9 months ago)

Hi

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OfflineFrankRhizo
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915584 - 04/04/19 02:25 PM (5 years, 9 months ago)

I use a 2in roll of saran wrap, double wrapped plate. Hasn't come off yet. Probably easier to take off than MP tape.

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: FrankRhizo]
    #25915611 - 04/04/19 02:39 PM (5 years, 9 months ago)

Definitely easier two take off than mp tape. Way way way easier. You don't really need two inch rolls though. i cut mine at about 3/4 of an inch. Just a smidge taller than a petri dish.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: FrankRhizo]
    #25915612 - 04/04/19 02:40 PM (5 years, 9 months ago)

This plate is joining the group. It’s is a piece of grain knocked up with a lc clone from this cluster maybe I can clean it up.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915615 - 04/04/19 02:41 PM (5 years, 9 months ago)

3/4 inch sounds like a plan. Thats way easier then what I have been doing.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915624 - 04/04/19 02:46 PM (5 years, 9 months ago)

I truely appreciate all the support I’ll put prints out to everyone that is interested and helps out. Atleast until I run out of them.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915632 - 04/04/19 02:52 PM (5 years, 9 months ago)

Quote:

Subfinder said:
I'd go spores to agar. Keep what you have going.

I'd skip the LC for now, stick to agar. Cut a wedge when it's cleaned up and drop it to grain.



Gonna drop some spores on agar right now just for you sense you have been answering this thread from the beginning.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915694 - 04/04/19 03:26 PM (5 years, 9 months ago)

1 drop Amazonia spores from a spore syringe.

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait] * 1
    #25915700 - 04/04/19 03:29 PM (5 years, 9 months ago)

nice. to up your chances of isolating clean growth, I'd suggesting making or buying an inoculation loop and using that to "streak" the agar. It helps.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25915712 - 04/04/19 03:33 PM (5 years, 9 months ago)

Awsome I’ll get one

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OfflineSubfinder
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915797 - 04/04/19 04:14 PM (5 years, 9 months ago)

Yup, do a zig-zag on the plate with a loop. I usually try to transfer a small bit before it grows out too much. The second and third transfers are the money transfers...youll start to see uniform growth.

Always drop your transfers to the center. I like to cut a transfer when it's about 3/4 spread out. Before it hits the edge of the plate.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917615 - 04/05/19 01:27 PM (5 years, 9 months ago)

Had to run errands all day today. The petris are taking off and I will post pics when I get home. I also had to order more petris today as I only have 8 made up so they will be here Monday. I didn’t want to wait for the inoculation loop or pay 20 shipping on a $3 item so I hit the hardware store and I’m going to rig one up I’ll post the project tonight.

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917738 - 04/05/19 02:33 PM (5 years, 9 months ago)

Thin wire, folded in half, both ends locked into a drill, small round object held in the fold of wire (small drill bit, Think: small enough to hold a drop), spin drill until it tightens up. Don't over do it or you'll snap the wire.

Be sure to make it long enough that you don't burn your hand when flaming or make a handle out of rtv.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25917752 - 04/05/19 02:38 PM (5 years, 9 months ago)

Cool idea I was also thinking about attaching it to the handle of my scalpel?

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917756 - 04/05/19 02:40 PM (5 years, 9 months ago)

By the way anyone that helps out and wants a print from this pm me so I can keep track

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917817 - 04/05/19 03:24 PM (5 years, 9 months ago)

Ok so here’s the situation I only have 7 new plates made up till mine show up in the mail Wednesday. I have two empty petris I could fill with agar if I get desperate. But I think we’ll be ok. So here’s photos of the plates I got going right now. It may be time for first transfers on some?
First three pics are pin clones from a cluster of a multi spore grow.(golden teacher) time to transfer some tips of let it grow out? the next 2 pics are from lc made from a cluster from an equador grow. They have satellite bacteria colonies and I’m pretty sure the second one should be discarded and maybe the mycelium grabbed and transferred from the first? next on the list is a piece of colonized grain from the same equador cluster lc and last but not least is the Amazon spores they haven’t taken off yet so the gt pin cluster was really nice and would be cool to see what happens but that equador cluster is amazing. If u missed it I have posted it multiple times previously in this thread. I don’t wanna loose that one. What do you guys think when would u start your first transfer and from what?

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917831 - 04/05/19 03:33 PM (5 years, 9 months ago)

Super simpler to make with just a screw driver and pliers

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OfflineSubfinder
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917898 - 04/05/19 04:08 PM (5 years, 9 months ago)

Let it grow out to about 3/4 of the plate. Then you give the contaminants time to show their ugly faces....then you transfer the good myc away from the contaminants.

Your plates are nowhere near ready to take transfers.

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917922 - 04/05/19 04:25 PM (5 years, 9 months ago)

I'd be hesitant to say for sure but i think those plates have some super heavy bacteria in them. Try uploading some back lit pictures of the first and second to last pics (that's where i see it the worst).

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