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thisbliss
Stranger

Registered: 02/27/19
Posts: 399
Last seen: 4 years, 7 months
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Storing fruit tissue
#25917221 - 04/05/19 09:44 AM (5 years, 9 months ago) |
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People how could I store a piece of mushroom meat so it would keep for at least a couple weeks and then I could clone it.
I'm waiting on agar equipment to arrive but I have a great cake fruiting a number of great flushes now that I think would be worthy of cloning.
I was thinking of just cutting a sterile section from the centre of a fruit and placing in a sterile jar. But this will probably mold in a week before I get to place on agar. Any ideas ? Thanks in advance
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Mr. Funguy
Quasi Frodo


Registered: 01/19/19
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Loc: Terra
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Re: Storing fruit tissue [Re: thisbliss]
#25917257 - 04/05/19 10:04 AM (5 years, 9 months ago) |
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Freezer is my best guess...
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SFS96
AstroMan



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Re: Storing fruit tissue [Re: Mr. Funguy] 1
#25917263 - 04/05/19 10:06 AM (5 years, 9 months ago) |
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Id just wait untill u have the agar stuff in honesty  Hopefully you will have some new fruits to clone by then.
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
Edited by SFS96 (04/05/19 10:07 AM)
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Buck513
Registered: 04/17/14
Posts: 5,682
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Re: Storing fruit tissue [Re: SFS96]
#25917286 - 04/05/19 10:20 AM (5 years, 9 months ago) |
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I would probably just wait as well. Maybe try some experiments with sterile water or something?
Dont freeze them either. When they start to thaw they’ll turn all nasty.
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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thisbliss
Stranger

Registered: 02/27/19
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Re: Storing fruit tissue [Re: SFS96]
#25917307 - 04/05/19 10:34 AM (5 years, 9 months ago) |
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True it's possible there'll be another flush after this one but cakes looking a bit spent.
I think I'll try the freezer sure why not. Also I could just put some in a new brf jar - that's like cloning right?
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mushboy
modboy



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Posts: 35,378
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Re: Storing fruit tissue [Re: thisbliss]
#25917318 - 04/05/19 10:39 AM (5 years, 9 months ago) |
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freezing fresh shrooms will fuck with the cell structure. i dont think youll want to clone thawed out liquid mushroom goop.
just wait. there will always be more clones to take
Quote:
thisbliss said: Also I could just put some in a new brf jar - that's like cloning right?
you could but i wouldnt. just be patient.
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UncleHoyphae
Curious noob



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Re: Storing fruit tissue [Re: mushboy]
#25917473 - 04/05/19 12:15 PM (5 years, 9 months ago) |
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Clone them now onto cardboard. Read up on the cardboard cloning tek, it's very simple and it does work. Once your agar arrives yu can transfer the healthy MYC from your cardboard to agar
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thisbliss
Stranger

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Thanks buds 👍
Must have a look at that cardboard.
Another thing I was wondering. Say I just let the cake sit about to keep the myc alive. Surely I can cut into the middle of it where it should still be sterile and take some myc from that? I know lot will probably frown at this but I'm thinking it might be a good project to learn on agar to clean it up - hone some skills
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UncleHoyphae
Curious noob



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Re: Storing fruit tissue [Re: thisbliss]
#25918394 - 04/05/19 08:34 PM (5 years, 9 months ago) |
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I'm a noob so not really sure RE brf cakes. I've read that a cake can look super healthy on the outside but be contaminated in the middle when you cut it in half. I don't think the inside is necessarily sterile.
Oh and with the cardboard cloning tek. One of them talks about dipping in hydrogen peroxide first. DON'T do that. I tried and it killed all my samples and no MYC grew
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thisbliss
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Sweet don't have any peroxide so was thinking of just skipping that stage lol
Might use the oven to sterilise some cardboard and then add sterile water to give it right consistency. Seems easiest. Should buy me a couple weeks
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UncleHoyphae
Curious noob



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Re: Storing fruit tissue [Re: thisbliss]
#25919035 - 04/06/19 08:08 AM (5 years, 9 months ago) |
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Just boil it, put it in a small jar and then steam sterilise that 👍
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thisbliss
Stranger

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Unclehoyphae cheers dude for the heads up about the cardboard Tek. Got it going and managed to preserve enough mycelium to inoculate my agar plates when they arrived. Here's one plate I've done. I'm super proud it's my first forage into agar!
url=https://files.shroomery.org/files/19-16/585441696-IMG_20190421_143027746.jpg][image]http://www.shroomery.org/forums/thumbs/19-16/585441696-thumb_IMG_20190421_143027746.j
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thisbliss
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Re: Storing fruit tissue [Re: thisbliss]
#25947097 - 04/21/19 08:44 AM (5 years, 8 months ago) |
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Yep
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UncleHoyphae
Curious noob



Registered: 03/31/19
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Re: Storing fruit tissue [Re: thisbliss]
#25948514 - 04/22/19 01:34 AM (5 years, 8 months ago) |
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Looks awesome dude! Well done 
Got my first EVER pins today (been doing agar work with dried mushys for my first ever grow) and I'm super excited!
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thisbliss
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👍 nice one. Some lovely mycelium action there. What you got going on in those tubs?
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UncleHoyphae
Curious noob



Registered: 03/31/19
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Re: Storing fruit tissue [Re: thisbliss]
#25950288 - 04/23/19 04:53 AM (5 years, 8 months ago) |
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I'm just top fruiting a spawn jar cos I wanted to try get fruits quickly and with minimal risk before a 2 week trip beginning may. It's whole brown rice with a coir casing
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thisbliss
Stranger

Registered: 02/27/19
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Oh yeah? Lot of nice hyphae knots and pins there. Do you put a tub or plastic bag over the top to hold humidity?
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UncleHoyphae
Curious noob



Registered: 03/31/19
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Loc: Vietnam
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Re: Storing fruit tissue [Re: thisbliss]
#25950884 - 04/23/19 01:20 PM (5 years, 8 months ago) |
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Those ones are in a zip loc bag/an old plastic French bread stick bag. I also have some others inside 1l plastic containers with perlite at the bottom.
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thisbliss
Stranger

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Good idea is that the french bag with the tiny holes in it? B perfect for fae. I use a lot of 2l soda bottles for FC among other things. Nearly best of the lot. Just a pita when fruits get big
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UncleHoyphae
Curious noob



Registered: 03/31/19
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Re: Storing fruit tissue [Re: thisbliss]
#25952550 - 04/24/19 10:14 AM (5 years, 8 months ago) |
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Nah, just a long plastic bag type of thing really, but the perforated one would be a great idea.
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