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Teach a newb to grow magic
    #25914147 -

Hey gals and guys, I have been having hella trouble figuring out the bulk in a mono. I don’t no what I am doing wrong but I get tons of pins that just never really mature or super short mature fruit or 1-2 really large fruits and nothing else. So I have had enough of this. Today I made agar (1 3/4 cups grain soak to 7.5 grams agar pressure cooked for 20 minutes) I poured petris and took 3 fresh pins off a small gt cluster with a scalpel in a still air box. I plan on letting mycelium grow to dime size and then doing a transfer. I’m going to post in this thread every time I make a move and ask for help each step of the way. I’m going to post what I have to work with and hope for as many suggestions as possible. I’m hoping with help I can stop failing at bulk. Please chime in at any point if u have a suggestion or an idea what to do next. I’m hoping this can be very helpful to myself and other people struggling to learn these methods. Maybe other people will even grow along with us. Here’s the tub I have to turn to a mono.
Materials list:
Coir, verm, peat moss, crushed oyster shell, lime, petris, agar, oats, canning jars, pressure cooker, Polyfil, and more.
I will be posting pics of the plates as they grow out and asking advice where to take my next transfer from. Thanks everyone

Edited by Hippiebait (04/03/19 06:17 PM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25914219 -

This should be cool.

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Re: Teach a newb to grow magic [Re: FrankRhizo]
    #25914245 -

I hope it turns out cool and I hope it’s very helpful

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25914456 -

What are you growing? cubes? pans??

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Re: Teach a newb to grow magic [Re: woofwoof]
    #25914482 -

:takingnotes:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: Teach a newb to grow magic [Re: woofwoof]
    #25914888 -

woofwoof said:
What are you growing? cubes? pans??


Golden teacher cubensis

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25914979 -

1 bag black kow cow shit added to available supplies. I don’t think I’ll need it but what do I know.

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Re: Teach a newb to grow magic [Re: Hippiebait] * 1
    #25915015 -

Jesus, cut half that shit off your list. Start out simple...just coir for your sub. All that other shit is not necessary for cubes. 650 grams of coir and 3.5 quarts of boiling water is all you need for cubes. All that other shit will only complicate your grow.

You need to establish a base line before you play with all the fancy stuff or you won't have anything to compare your results to.

Simple is better.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915172 -

So here’s the coir I been experimenting with. Is it ok? Mycelium does eat it up. Also I’m in northern Maine it is very very very dry is that matters any.

Edited by Hippiebait (04/04/19 09:25 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915197 -

I know this is to much direct light it’s here for photo purpose only. But what do y’all think of the tub design. It’s got a seal in the lid so no extra air flow. The filters on the side I made by ironing 4 layers of this together. Keep it or start over? (The actually pic is of an old project that still may fruit) I have more tubs I have not modified yet

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915211 -

It’s very hard to see but on Top two plates the mycelium is starting to jump onto the agar.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915245 -

Dry air is something we all deal with in the north. Just requires an occasional mist to keep the surface looking right.

The polyfill will work but 3M micropore tape is much easier and cleaner looking.

I'd cut that liner down so it's even with the surface of your substrate.

Don't overthink the fruiting part. Concentrate on clean mycillium on agar. Get that to grain and fruiting will happen. Clean spawn fruits easy.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915253 -

I have 3m tape how do u recomend I use it that would be way easier! And really cut the liner all the way to the sub? I left extra because I figured I needed for a casing🤷‍♂️ Do you recomend not casing or does it just not matter if the liner is below the casing? I’m on top of that agar I’m taking more clones tonight or tommorrow and I will post them

Edited by Hippiebait (04/04/19 10:00 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915352 -

Cubes do not need casing. Like I said, best to keep it simple as possible and experiment later after you have some successful grows under your belt. I use a single layer of mp tape on the bottom holes, 2 layers on the top. The tape isn't wide enough for 3/4" holes, so you'll have to overlap the tape slightly.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915354 -

How are you cloning? Pin from agar? I like to grow out multispore and take a fruit from the best clusters and take a tissue sample from inside the stem.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915361 -

Subfinder said:
Jesus, cut half that shit off your list. Start out simple...just coir for your sub. All that other shit is not necessary for cubes. 650 grams of coir and 3.5 quarts of boiling water is all you need for cubes. All that other shit will only complicate your grow.

You need to establish a base line before you play with all the fancy stuff or you won't have anything to compare your results to.

Simple is better.


Agree 100%


--------------------
“You don’t have a soul, you are a sole. You have a body.”
C.S. Lewis

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Re: Teach a newb to grow magic [Re: Leon Sabanna]
    #25915381 -

So everything I have come from a multi spore originally. I have lc of multispore Amazon, b+, equador, and golden teacher. I have lc from cluster clones of equador, b+, and golden teacher. I also have pins and fresh fruit from all 3 at my disposal. This is equador I grew on cakes and cloned the cluster to lc I’m trying to grow that out on agar. Here’s my progress I was gonna add these to the thread later tonight.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915384 -

My holes have been 2 Inch is this to big should I cut back to 3/4?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915406 -

I've only run 3/4" holes. 2" seems awfully big. If it doesn't dry the surface too fast it should be good.

I can't tell what I'm looking at with your plates. Kinda looks like some satellites of bacteria colonies popping up on your agar. Could just be my bad interpretation of your photo.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915414 -

I think you may be right with the liquid culture clones. The tape came undone a little and I think they got some fresh air. Should I grab the mycelium that’s forming and transfer it or just start over on those plates.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915496 -

I'd go spores to agar. Keep what you have going.

I'd skip the LC for now, stick to agar. Cut a wedge when it's cleaned up and drop it to grain.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915517 -

Hi


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915584 -

I use a 2in roll of saran wrap, double wrapped plate. Hasn't come off yet. Probably easier to take off than MP tape.

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Re: Teach a newb to grow magic [Re: FrankRhizo]
    #25915611 -

Definitely easier two take off than mp tape. Way way way easier. You don't really need two inch rolls though. i cut mine at about 3/4 of an inch. Just a smidge taller than a petri dish.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: FrankRhizo]
    #25915612 -

This plate is joining the group. It’s is a piece of grain knocked up with a lc clone from this cluster maybe I can clean it up.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915615 -

3/4 inch sounds like a plan. Thats way easier then what I have been doing.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915624 -

I truely appreciate all the support I’ll put prints out to everyone that is interested and helps out. Atleast until I run out of them.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25915632 -

Subfinder said:
I'd go spores to agar. Keep what you have going.

I'd skip the LC for now, stick to agar. Cut a wedge when it's cleaned up and drop it to grain.


Gonna drop some spores on agar right now just for you sense you have been answering this thread from the beginning.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915694 -

1 drop Amazonia spores from a spore syringe.

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Re: Teach a newb to grow magic [Re: Hippiebait] * 1
    #25915700 -

nice. to up your chances of isolating clean growth, I'd suggesting making or buying an inoculation loop and using that to "streak" the agar. It helps.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25915712 -

Awsome I’ll get one

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25915797 -

Yup, do a zig-zag on the plate with a loop. I usually try to transfer a small bit before it grows out too much. The second and third transfers are the money transfers...youll start to see uniform growth.

Always drop your transfers to the center. I like to cut a transfer when it's about 3/4 spread out. Before it hits the edge of the plate.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917615 -

Had to run errands all day today. The petris are taking off and I will post pics when I get home. I also had to order more petris today as I only have 8 made up so they will be here Monday. I didn’t want to wait for the inoculation loop or pay 20 shipping on a $3 item so I hit the hardware store and I’m going to rig one up I’ll post the project tonight.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917738 -

Thin wire, folded in half, both ends locked into a drill, small round object held in the fold of wire (small drill bit, Think: small enough to hold a drop), spin drill until it tightens up. Don't over do it or you'll snap the wire.

Be sure to make it long enough that you don't burn your hand when flaming or make a handle out of rtv.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25917752 -

Cool idea I was also thinking about attaching it to the handle of my scalpel?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917756 -

By the way anyone that helps out and wants a print from this pm me so I can keep track

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917817 -

Ok so here’s the situation I only have 7 new plates made up till mine show up in the mail Wednesday. I have two empty petris I could fill with agar if I get desperate. But I think we’ll be ok. So here’s photos of the plates I got going right now. It may be time for first transfers on some?
First three pics are pin clones from a cluster of a multi spore grow.(golden teacher) time to transfer some tips of let it grow out? the next 2 pics are from lc made from a cluster from an equador grow. They have satellite bacteria colonies and I’m pretty sure the second one should be discarded and maybe the mycelium grabbed and transferred from the first? next on the list is a piece of colonized grain from the same equador cluster lc and last but not least is the Amazon spores they haven’t taken off yet so the gt pin cluster was really nice and would be cool to see what happens but that equador cluster is amazing. If u missed it I have posted it multiple times previously in this thread. I don’t wanna loose that one. What do you guys think when would u start your first transfer and from what?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917831 -

Super simpler to make with just a screw driver and pliers

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917898 -

Let it grow out to about 3/4 of the plate. Then you give the contaminants time to show their ugly faces....then you transfer the good myc away from the contaminants.

Your plates are nowhere near ready to take transfers.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917922 -

I'd be hesitant to say for sure but i think those plates have some super heavy bacteria in them. Try uploading some back lit pictures of the first and second to last pics (that's where i see it the worst).


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25917930 -

I thought so too. Looks like satellite colonies.

Still potentially salvageable. You'll need to work on your sterile technique. Never allow your hands or tools to go over your work.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917962 -


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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917970 -

So this is the recommended tub design? Little 3/4 inch holes? They are placed right correct?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918047 -

tub looks about right but it seems like your holes are super high. How high is that line?

And your plates.... I hate to be the one to inform you, but.... Things aren't looking too good my friend. I see plenty of bacteria in a lot of your dishes but most dominantly in the first. You see all that weirdness around the pin. Was that there before you put the pin in there? That's either a serious bacterial bloom or the sneakiest lighting trick ever. you may be able to snatch some myc up from the 6 oclock position after and if it outgrows the bacteria. Don't jump the gun too much. Wait for the myc to clear the bacterial goo.

Even then you may pick some up that is riding along with the myc. a  few more transfers and you  may be able to clean it up.

the second pick looks like it maybe doing well but i can't tell due to the blur. Third pick looks alright but definitely has a little bacterial colony at the 7 oclock position. This means there is probably more lurking around.

third and fourth LC pics look totally fucked. If that is a test from an LC i'd recommend trashing the LC.

I can't tell much of anything from the last two pics.

I understand that the pins are  from an open grow and that they are not going to be clean, but the amount of bacteria on the first plate is highly concerning and almost certainly did not come from that pin (in my opinion)

subfinder, the advice to "let it grow out 3/4 of the plate" is not generally considered to be "correct". When working to get away from contams, i like to snatch it up as soon as it sufficiently clears the contam. This is never the same, plate to plate. Sometimes i'm snatching up clean myc while the contaminated side of the transfer hasn't even leapt off the wedge yet.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


Edited by verum subsequentis (04/05/19 05:38 PM)

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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918079 -

First pic is a close up of the lc. The second two are of the gt pin clones that may be able to be saved. The holes on the tub are 4” high is that to high?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918128 -

The holes will be fine at 4 inches. I don't know why they looked so high to me. Your agar has bacteria all over the place. maybe you aren't pressure cooking it well enough. How'd you go about cooking the agar?


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918131 -

I mixed it well put it in a mason jar and I cooked it on high in my presto for 15 minutes and then gave it time to cool before pouring in a Sab

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918414 -

Do you have any signs of growth on leftover uninnoculated plates?


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918846 -

They are in a ziplock in the fridge should I take them out and watch? There’s deffinatly some plates that are horrible with bacteria but some look clean to me🤷‍♂️ Do I just not know what I’m looking at or could it be that you are all seeing some dust and some left over Lysol on the outside of the dishes? I’m gonna take up close pics and seperate what I “think is clean and what I think is not”

Edited by Hippiebait (04/06/19 06:37 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918915 -

Some of the mycelium in a few of my plates has outrun the bacteria unless I’m missing something. I’ll post pics in a little while and see if I should transfer yet

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918975 -

So to me “after cleaning the outside of the petris of Lysol.” It looks like the first 4 are relatively clean besides maybe a little spec or two? The last 3 are trash?

Should I begin transfers from the first two pins and the grain? if so Where from? The grain is the truely amazing equador cluster I would love to get going!! The two pins are from a big cluster of golden teacher that wasn’t aloud to mature🤷‍♂️

Edited by Hippiebait (04/06/19 07:29 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919022 -

U got bacteria all over the place. Ur best bet would be to let those first 3 pin clones grow out for another day then transfer.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919040 -

Thanks! That’s what I was thinking but unsure. The last 3 plates I may as well just throw away correct?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919043 -

Definitely. It's not even worth it. But atleast there isn't TOO MUCH bacteria on the first 3. Just avoid it when u take transfers. Before u transfer, always hold the plate up to the light so u can see if there's bacteria hidden under the mycelium.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919052 -

That makes sense for sure! I have the genetics I would like to use on the first plate and the second best on the second two plates so that works out.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919067 -

What would u folks do wit 40 quarts of grain u didn’t clean up on agar but recover with the shake test? Should I throw them all in a tub and cover with damp verm and hope for the best?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919072 -

40 quarts? U can make like 8-10 monos... pics? If they're clean fruit them. If they don't look good, throw them out :shrug:

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Re: Teach a newb to grow magic [Re: nube424]
    #25919101 -

They fully recover in 48 hours after a shake but I don’t want to even waste the materials if it’s most likly just an experiment as I have plenty of cakes going I’ll just wait for a clean culture but if I could get a pound or so off those grains it’s worth growing them out.

Edited by Hippiebait (04/06/19 09:01 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919126 -

That's a pint jar... not a quart. But Yea, I would grow them out as long as they're not contaminated.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919132 -

I’ll give it a try. I was gonna just throw them away cause it seams like I get horrible results as far as ms grows go. Maybe I’ll just go with a super high spawn ratio🤷‍♂️

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919137 -

Hippiebait said:
What would u folks do wit 40 quarts of grain u didn’t clean up on agar but recover with the shake test? Should I throw them all in a tub and cover with damp verm and hope for the best?


And back to this, I wouldn't just dump them in a tub and cover with verm... can u get coir? I would mix with coir and throw it in some monos. 4 bricks of coir with 40 pints would work.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919143 -

:thumbup:

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Re: Teach a newb to grow magic [Re: nube424]
    #25919156 -

nube424 said:
Hippiebait said:
What would u folks do wit 40 quarts of grain u didn’t clean up on agar but recover with the shake test? Should I throw them all in a tub and cover with damp verm and hope for the best?


And back to this, I wouldn't just dump them in a tub and cover with verm... can u get coir? I would mix with coir and throw it in some monos. 4 bricks of coir with 40 pints would work.


Do you mix everything first and then put it in the mono? This sounds way easier then trying to mix each individual mono but wouldn’t it spread and contams there may be?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919173 -

There shouldn't be any contams in any jars u spawn 😐 and they don't look very bacterial. Do it however u wish. If I was spawning that much I would just mix all of it and put it in the monos after. Depends how confident u are. The biggest problem I see people do is making their coir (Or any bulk sub) too wet. When voir is at field capacity, it will be nice and fluffy and it wont feel wet. And ull only get a couple drops of water from squeezing a handful. I like to make mine on the dry side, it's easier to add water after than take it out.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919177 -

Cool I’m gonna give these jars a shot after all. You saved them from the garbage or a sabotage experiment 🧪.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919202 -

nube424 said:
There shouldn't be any contams in any jars u spawn 😐 and they don't look very bacterial. Do it however u wish. If I was spawning that much I would just mix all of it and put it in the monos after. Depends how confident u are. The biggest problem I see people do is making their coir (Or any bulk sub) too wet. When voir is at field capacity, it will be nice and fluffy and it wont feel wet. And ull only get a couple drops of water from squeezing a handful. I like to make mine on the dry side, it's easier to add water after than take it out.


What about contams that enter the sub while transferring to the mono? Am I understanding that maybe this doesn’t need to be as sterile as the spawn prep? I usually try to mix everything quick after i sterilize the mono best I can and then get the lid shut? Now I feel like that’s a lot of drama for nothing?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919262 -

A whole lot of drama for nothing lol. Just the agar/spawn making process needs to be sterile. Once ur jars are colonized u don't need to be sterile anymore. It's a whole lot to explain on here. But basically ur bulk substrate is naturally contams resistant. Grains, are contam magnets. But once grain is colonized mycelium can fight things off. So when u spawn ur tubs, d ont worry about contamination getting in. U could sneeze on a monotub and it would be fine.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919289 -

good info!!

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Re: Teach a newb to grow magic [Re: nube424]
    #25919299 -

If those jars came from the same syringe that is showing tons of bacteria on agar, I'd just throw a healthy top layer of coir in each pint, place as many as possible in tubs and mist as needed.

I can't tell from the picture of the grain but if they're heavily bacterial you'll have much more luck hunting for clones if you top fruit them.

And yes, as nob said, high nutrition = high contam possibilities. Spawn, agar, LC... are all very susceptible to contams because they are very high in nutrition. We use coir as a substrate specifically because it holds a ton of water and is low in nutrition.

It is a good idea to be as clean as practically possible while spawning tubs but you really don't have to. I often spawn bare handed without showering or putting n freshly laundered clothes first.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25919330 -

The jars and from a multispore syringe different from any of the plates. I thought the jars looked pretty clean myself and they break up easy and rocolonize fast so I’m figuring I should give them a try? And great to no about preparing the tubs! I will still be careful but all that extra work takes a lot of time.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919381 -

If they look clean and break apart easy I'd say ur good to go. Just inspect them all before u spawn them. U seem kinda anal tho so I'm sure u would have noticed mold already lol. No offense.

Sometimes u can't go by what other people say. Gotta use ur own judgment and just see what happens. I've had plenty of people say my jars were bad but everything went just fine.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919477 -

truth


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25919508 -

I’ve looked over them pretty well just as u suspected! Lol. I don’t see anything wrong with them so I’m gonna go for it.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919515 -

The 3 good trays really eating up the agar

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919549 -

Those are actually lookin pretty good. right on.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25919591 -

😎

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919938 -

The 3rd one looks like a sperm :rolleyes:

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919946 -

So on a mono tub do u guys wait for consolidation before fruiting or just colonization? If so how long I usually do my cakes till I see knots.

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Re: Teach a newb to grow magic [Re: nube424]
    #25919949 -

nube424 said:
The 3rd one looks like a sperm :rolleyes:


Lol it’s a colonized piece of grain

Edited by Hippiebait (04/06/19 06:39 PM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25920011 -

i usually introduce my tubs to fruiting conditions right when i spawn them. Works like a charm. When you see knots on cakes is all about what genetics you have.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25920021 -

Cool I have been taping the insides of the holes with packing tape and letting it colonize. Yet another unneeded step🤦‍♂️

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25920029 -

not everyone agrees but i like it.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25920037 -

I’ll have to try it

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25920714 -

I do believe it’s transfer time. Maybe not from the grain it’s a day behind but the 2 I believe need one?
Does it matter where I start long as it’s clean?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921126 -

sure. always look very very closely before transferring. Can you see the bacteria in the third picture. If not, look harder. take a transfer from the right side (3 oclock) and you should be good


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25921176 -

I do see what may be bacteria but honestly I think it’s just were I sliced the agar because I couldn’t get the clone off the scalpel lol. Now I might be right though bacteria may have grown in that slice. There are some little spots of bacteria appearing around the grain plate and that sucks because that’s the one I really want to save.

Edited by Hippiebait (04/07/19 11:34 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921182 -

By the way I took a look and your right there’s little spots of bacteria growing at the end of the slices in the agar.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921205 -

i know. You have to learn to see these things or you'll transfer until your arms fall off and never get clean cultures.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25921223 -

You can learn a lot by observing your plates. Take some time and study the appearance of the myc that is close to the bacteria and the myc on the other side. It's a perfect real life example of how to spot contam weakened myc. You can learn to know if there is some bacteria (that you can't even see) by learning what clean healthy myc looks like.

That third plate shows two great examples. The one part, by the bacterial scalpel slice, is showing you weak growth. This is indicative of either poor genetics (that you don't want anyway) or some myc that's fighting something off.  Just above that you see another prime example. Disorganized/ chaotic/ un uniform growth. This (in my opinion) is indicative of the myc trying to grow away from the bacteria and growing off the agar and over itself.

This is why you'll hear folks who know their mycology say, "take a small, careful transfer from the leading edge of the fastest, healthiest, most organized myc"


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25921258 -

That all makes perfect sense when explained like that. Now I just have to learn to spot it.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921571 -

Before and after

It was hard to get the mycelium agar off the loop. Tricks? I can already see how I took from the wrong spot on the first tray.🤦‍♂️

Edited by Hippiebait (04/07/19 04:19 PM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921618 -

you don't take transfers with a loop. Scalpels are for agar transfers and loops are for spores. I have no tips for getting agar off a loop because i've never done it.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921630 -

If ur transfer tool is hot, it melts the agar and makes it hard to get off. Try cutting while it's hot, then allow a few seconds for it to cool before u scoop it up.

And yes, the first plate has alot of good transfers. I'd keep that one for a few more days and see if u want to transfer anything else from it

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Re: Teach a newb to grow magic [Re: nube424]
    #25921646 -

Im deff gonna keep all the plates for now. I have 40 more petris showing up Wednesday so I’ll have more to work with by Thursday. On a side note how does this look. It’s ms golden teacher from pf tek to bulk.

Edited by Hippiebait (04/07/19 06:30 PM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25921879 -

I gotta follow this, learning so much


--------------------

Crack's Tek's and Shit


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Re: Teach a newb to grow magic [Re: Crackatoa]
    #25922007 -

the ucolonized portions are concerning. it's not normal to see hyphal knots along side uncolonized substrate unless contams are present. Most likely bacteria (which may not ruin your grow) but not a good sign. Otherwise, as far as i can tell, it looks alright.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25922384 -

I may be wrong but I don’t see any hyphal knots. That tub was just spawned 5 days ago. There’s lots or verm with lots of tiny little white chunks? Am I missing the knots? This tub is however half verm. It was made just before y’all told me to just use coir.

Edited by Hippiebait (04/08/19 05:48 AM)

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Re: Teach a newb to grow magic [Re: Crackatoa]
    #25922426 -

Crackatoa said:
I gotta follow this, learning so much


I’m glad, that was the goal for myself and others to learn.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25922487 -

Hippiebait said:
I may be wrong but I don’t see any hyphal knots. That tub was just spawned 5 days ago. There’s lots or verm with lots of tiny little white chunks? Am I missing the knots? This tub is however half verm. It was made just before y’all told me to just use coir.


Yeah there wont be any hyphal knots yet. Those chunks can definitely be mistaken for them though.

Time will tell. It's weird that its skipping those smaller portions. But line I said.. time will tell. And I wish you luck on this one here!


--------------------
:greyalien:



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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25922493 -

Hippiebait said:
So here’s the coir I been experimenting with. Is it ok? Mycelium does eat it up. Also I’m in northern Maine it is very very very dry is that matters any.


Mmmmmm use bricks

Ye claimed matters
Don't worry you'll figure it out.


--------------------


Nothing breads nothing

Edited by Geinstein (04/08/19 01:15 PM)

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Re: Teach a newb to grow magic [Re: Geinstein]
    #25922860 -

damn, those aren't knots? This is why I'm hesitant to tell people what's up with their pictures. I looked at it again.... and again, and it really looks like knots. 5 days is quick though. It could be pinning early to try and survive what could be lurking in the uncolonized portions. But, now that i know how young it is, it's possible that those are just chunks of coir that didn't get broken up. Myc takes a while to eat through chunks of unbroken coir.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25923147 -

It looks a lot like knots in the picture that for but I think it’s just continuing to colonize as normal. This is where it usually goes all haywire for me though. I usually end up with 1-2 really big fruits and tons of fruits to tiny to harvest.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25923175 -

My transfers aren’t doing anything yet. I may be jumping the gun but nothing is growing yet. Gonna try a few more tonight. I wonder if I fried it all with my hot loop🤷‍♂️

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25923179 -

Try to take SLIGHTLY bigger pieces and try not to melt the agar lol

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Re: Teach a newb to grow magic [Re: nube424]
    #25923302 -

Will do👍🏻

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Re: Teach a newb to grow magic [Re: Hippiebait] * 1
    #25923582 -

Took some more transfers but I’ll be very surprised if h
They are clean I need practice badly.

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Re: Teach a newb to grow magic [Re: Hippiebait] * 1
    #25923587 -

Hippiebait said:
Took some more transfers but I’ll be very surprised if h
They are clean I need practice badly.


Just imagine that your hands have a pile of baby powder on them (tools too) and your trying to do each step without spilling any of the baby powder into your work.

You could actually practice this way if you wanted!

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25923656 -

It’s just really hard to see what I’m doing inside my still air box. And I have trouble getting the transfers off my tools.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25923693 -

Put a light on top of your SAB. It takes a feel to get the poke right for picking up a wedge and being able to stick the landing.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25923707 -

So practice!
Hippiebait said:
It’s just really hard to see what I’m doing inside my still air box. And I have trouble getting the transfers off my tools.


What's ur agar recipe? Is it kinda soft like jello? Stiffer agar doesn't stick like that.

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Re: Teach a newb to grow magic [Re: Subfinder] * 1
    #25923809 -

Just imagine that your hands have a pile of baby powder on them (tools too) and your trying to do each step without spilling any of the baby powder into your work.

You could actually practice this way if you wanted!


That’s some really good advice! I’m gonna try start thinking of my SAB work like that.


--------------------
“You don’t have a soul, you are a sole. You have a body.”
C.S. Lewis

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Re: Teach a newb to grow magic [Re: Leon Sabanna]
    #25924198 -

Yes my agar is soft. I’m pouring more in the am I’ll try a little firmer thanks!

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25924203 -

I'm using less and less agar every day
I'm at 12g per liter and it holds firm. Going to do 10g per liter next

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Re: Teach a newb to grow magic [Re: Geinstein]
    #25924458 -

So my first transfers that I thought I fryed  with the loop are showing life!! Not enough to photo yet but the mycelium has started to crawl.

Edited by Hippiebait (04/09/19 07:27 AM)

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25924791 -


I received my Petri dishes last night so I will be pouring this evening. Gonna stiffen it up with 10g agar and 1 3/4 cups water instead of 7.5 grams agar. Do y’all think any of these fruits are worthy of a fresh inside tissue clone? Not much for clusters maybe a few but it was grown on a bulk sub and then cased and only fruited like a cake because the tub it was in sucked. So in theory it’s fruiting conditions are one step closer to the main goal?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25925003 -

How do u make ur agar? U could just put 10g agar 10g nutes in a mason jar and fill it to the 500ml line and pc it. Agar is supposed to make up around 2% of the total liquid, so it's easier to calculate recipes if u use ml as a measurement.

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Re: Teach a newb to grow magic [Re: nube424]
    #25925032 -

I have just been using a mason jar in the pc and the pouring in a sab. I use straight grain soak water and agar.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25926248 -

Just poured 20 fresh plates!! Gonna take about ten transfers to try and get something clean. That will leave me with 30 plates. I hope that’s enough as I’m purchasing a food trailer and so I’m gonna be on the broke side for a while. That’s ok though I own my land and house and now I will own my own business. Plus one kick ass hobby!!!!! Anyways im gonna do my best to make my supply’s last for now but I will purchase the necessities for sure.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25926375 -

Did my transfers. I think I got it right and will have plenty of good plates on these transfers.😎 they went much smoother then previous attempts. Will post pics later if my earlier transfers. They may have some good spots but not like the recent.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25926669 -

right on man! you'll have an easier time pouring agar if you switch from a mason jar to a long neck whiskey bottle. It makes pouring way smoother. I use a captain morgan handle but any glass liquor bottle with a neck will be superior to a mason jar.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25926794 -

Hi Everyone!

I'm browsing the forum for a while and my eyes were grabbed by the grass seed tek. Thankfully with PF tek i had plenty of success before! :smile: So with agar i succeeded to colonize 8 little jars with no contams. Then i made the 50/50+ casing, the recipe was same what is used for the PF cakes. I used boxes inside the terrarium for growing. Put a 1.5 cm casing layer on the bottom, 4 cm colonized grass seed in the middle and 1 cm casing on the top. After two day the mycelium started to take over the casing and about two weeks later i got my first flush. And here is my question.. i got only 3-4 tall and normal cap sized mushrooms and a few aborts. After the first flush its giving 1-2 normal mushrooms about every week. I think i missed something with this tek.. :frown: Used shotgun terrarium with 4-5 cm perlite layer on the bottom. On the top of the perlite the boxes were put. Maybe you can give me some advice what did i do wrong..


Here is the box what i talked about:


Here is a strange one, which was growed on pure grass seed (no casing or whatsoever, just watering the bottom of its container). I wonder why it get so fuzzy :smile:


Many thanks!


--------------------
Save water, dilute it!

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Re: Teach a newb to grow magic [Re: Inorpos]
    #25926920 -

Never thought of a liquor bottle. Shame I don’t drink. So what you just cover it with tinfoil to pressure cook?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25927050 -

So most of my fist transfers were totally fucked so I didn’t bother photoing them all. However these 2 plates may make it. I beleive the few black spots to be sediment from the grain soak and should be sterile.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25927386 -

How does one know when a plate is clean enough/ enough transfers in to take grain. If my new t’1s I just did tonight turn up super clean are they good to go? Do I need to be on atleast t’2 or t’3 to assume it’s really clean dispite appearance?

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25927394 -

Let them grow out more. It's better to let them spread some so you can see the cleanest looking growth. Plus, you'll give hidden contams a chance to show themselves. Look at some of the agar pics on the agar threads. You'll see lot's of good examples.

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Re: Teach a newb to grow magic [Re: Subfinder] * 1
    #25927620 -

good advice. you just have to learn what clean myc looks like. The problem is, it can look a few different ways. Most of the time contams are pretty obvious but some times they are very sneaky. The best way any of us have ever learned to put it in words, in my opinion, is "clean myc looks.... clean and organized". I think organized is a great word for it. One reason that a few tiny transfers is a good idea is that it slims down the genetic variation enough that you are not having to look at myc on top of myc on top of myc on top of ... All of these layers make it kind of hard to tell clean myc from mostly clean myc with a little bacteria or mold in it.... Or just all mold. "thinning the herd" makes organized and clean myc much easier to see.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis] * 1
    #25928097 -

These detailed descriptions keep helping tremendously!!! I new to let it grow out but wasn’t sure about more transfers. Now I know though!!

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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25928111 -

FrankRhizo said:
I use a 2in roll of saran wrap, double wrapped plate. Hasn't come off yet. Probably easier to take off than MP tape.


verum subsequentis said:
Definitely easier two take off than mp tape. Way way way easier. You don't really need two inch rolls though. i cut mine at about 3/4 of an inch. Just a smidge taller than a petri dish.


This was amazing advice. It took half of the ducking around out of making my plates making them way less likly to be contained. Thanks

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25928840 -

Do these jars look contamed or just really rhizo? Got a few like this

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25928857 -

When taking pics for id purposes. Take a few and look closely at them. If you can't tell what the hell you're looking at, we certainly won't be able to. I can't tell you much from those pics. I don't think it looks good but the light reflections are really fucking up my view.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25928883 -


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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25928893 -

Almost certainly fucked. Cultures can sometimes d strange things and be fine but it looks very problematic. Those super rizho shoots are almost definitely trying t get away from the mold (and bacteria) that are colonizing those grains. If you want, let it grow out 90 % and then shake the bastard and see how it recovers.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25928965 -

Their 90/more now I’ll shake them all. They are g2g from what I beleive to be somewhat clean but who knows I’m still learning.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25929259 -

So the jars broke up easy and shook well. They were mostly rhizo where eating up the uncolonized last little bits. Well see🤷‍♂️ Anyways I feel as if my transfers are making progress. Hopefully after 1 or 2 more transfers I’ll be clean neat and organized. Here’s the progress.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25929796 -

that's lookin pretty good man. nice


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25929902 -

Sweetness!!!

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25930014 -


I think this one looks even better.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25930026 -

You're certainly heading the right direction now. Let that plate grow out to the size of a quarter to 50 cent piece, and post a couple good clear pics and we'll help you decide where to take transfers from.

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Re: Teach a newb to grow magic [Re: Subfinder]
    #25930044 -

Cool I have a bunch of plates that are clean like this just a few days behind so the fun is about to start!! Atleast for a little while.

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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25932412 -

Hey all I got a few plates that may be ready for transfer🤷‍♂️ I have about 28 plates left and I’m trying to limit spending. Although I will buy plates if I need to. I have lots of clean t’1s that are a few days behind also so I really don’t want to transfer junk as I don’t need to. So where would u transfer from if u would.
Golden teacher clone. 2 plates.

Equador cluster clone. I have 3-4 clean t1s just 2 days behind.

B+. This is from a big fruit I cloned and then grew out on brf. I put it on this crappy plate of chunky agar with lots of sediment that I thought was for sure no good. Surprise!!! No contams as of yet and it’s clean neat and organized!! Thoughts?

Can I make transfers right from this b+ in a few days and spawn with those transfers long if it’s clean?

Edited by Hippiebait (04/13/19 12:53 PM)

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