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Hippiebait
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Registered: 01/06/19
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Cool I have been taping the insides of the holes with packing tape and letting it colonize. Yet another unneeded step🤦‍♂️
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verum subsequentis
seeker of truth



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Re: Teach a newb to grow magic [Re: Hippiebait]
#25920029 - 04/06/19 07:21 PM (5 years, 9 months ago) |
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not everyone agrees but i like it.
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Hippiebait
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I’ll have to try it
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Hippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25920714 - 04/07/19 05:48 AM (5 years, 9 months ago) |
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I do believe it’s transfer time. Maybe not from the grain it’s a day behind but the 2 I believe need one?
Does it matter where I start long as it’s clean?
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verum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921126 - 04/07/19 11:05 AM (5 years, 9 months ago) |
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sure. always look very very closely before transferring. Can you see the bacteria in the third picture. If not, look harder. take a transfer from the right side (3 oclock) and you should be good
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Hippiebait
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I do see what may be bacteria but honestly I think it’s just were I sliced the agar because I couldn’t get the clone off the scalpel lol. Now I might be right though bacteria may have grown in that slice. There are some little spots of bacteria appearing around the grain plate and that sucks because that’s the one I really want to save.
Edited by Hippiebait (04/07/19 11:34 AM)
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Hippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921182 - 04/07/19 11:35 AM (5 years, 9 months ago) |
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By the way I took a look and your right there’s little spots of bacteria growing at the end of the slices in the agar.
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verum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921205 - 04/07/19 11:48 AM (5 years, 9 months ago) |
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i know. You have to learn to see these things or you'll transfer until your arms fall off and never get clean cultures.
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verum subsequentis
seeker of truth



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You can learn a lot by observing your plates. Take some time and study the appearance of the myc that is close to the bacteria and the myc on the other side. It's a perfect real life example of how to spot contam weakened myc. You can learn to know if there is some bacteria (that you can't even see) by learning what clean healthy myc looks like.
That third plate shows two great examples. The one part, by the bacterial scalpel slice, is showing you weak growth. This is indicative of either poor genetics (that you don't want anyway) or some myc that's fighting something off. Just above that you see another prime example. Disorganized/ chaotic/ un uniform growth. This (in my opinion) is indicative of the myc trying to grow away from the bacteria and growing off the agar and over itself.
This is why you'll hear folks who know their mycology say, "take a small, careful transfer from the leading edge of the fastest, healthiest, most organized myc"
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Hippiebait
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That all makes perfect sense when explained like that. Now I just have to learn to spot it.
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Hippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921571 - 04/07/19 04:08 PM (5 years, 9 months ago) |
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Before and after
 It was hard to get the mycelium agar off the loop. Tricks? I can already see how I took from the wrong spot on the first tray.🤦‍♂️
Edited by Hippiebait (04/07/19 04:19 PM)
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verum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921618 - 04/07/19 04:37 PM (5 years, 9 months ago) |
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you don't take transfers with a loop. Scalpels are for agar transfers and loops are for spores. I have no tips for getting agar off a loop because i've never done it.
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nube424

Registered: 12/03/17
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921630 - 04/07/19 04:42 PM (5 years, 9 months ago) |
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If ur transfer tool is hot, it melts the agar and makes it hard to get off. Try cutting while it's hot, then allow a few seconds for it to cool before u scoop it up.
And yes, the first plate has alot of good transfers. I'd keep that one for a few more days and see if u want to transfer anything else from it
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Hippiebait
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Re: Teach a newb to grow magic [Re: nube424]
#25921646 - 04/07/19 04:54 PM (5 years, 9 months ago) |
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Im deff gonna keep all the plates for now. I have 40 more petris showing up Wednesday so I’ll have more to work with by Thursday. On a side note how does this look. It’s ms golden teacher from pf tek to bulk.
Edited by Hippiebait (04/07/19 06:30 PM)
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Crackatoa
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25921879 - 04/07/19 07:29 PM (5 years, 9 months ago) |
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I gotta follow this, learning so much
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verum subsequentis
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Re: Teach a newb to grow magic [Re: Crackatoa]
#25922007 - 04/07/19 09:01 PM (5 years, 9 months ago) |
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the ucolonized portions are concerning. it's not normal to see hyphal knots along side uncolonized substrate unless contams are present. Most likely bacteria (which may not ruin your grow) but not a good sign. Otherwise, as far as i can tell, it looks alright.
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Hippiebait
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I may be wrong but I don’t see any hyphal knots. That tub was just spawned 5 days ago. There’s lots or verm with lots of tiny little white chunks? Am I missing the knots? This tub is however half verm. It was made just before y’all told me to just use coir.
Edited by Hippiebait (04/08/19 05:48 AM)
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Hippiebait
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Re: Teach a newb to grow magic [Re: Crackatoa]
#25922426 - 04/08/19 06:14 AM (5 years, 9 months ago) |
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Quote:
Crackatoa said: I gotta follow this, learning so much
I’m glad, that was the goal for myself and others to learn.
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Vibe_Enthusiast
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25922487 - 04/08/19 07:06 AM (5 years, 9 months ago) |
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Quote:
Hippiebait said: I may be wrong but I don’t see any hyphal knots. That tub was just spawned 5 days ago. There’s lots or verm with lots of tiny little white chunks? Am I missing the knots? This tub is however half verm. It was made just before y’all told me to just use coir.
Yeah there wont be any hyphal knots yet. Those chunks can definitely be mistaken for them though.
Time will tell. It's weird that its skipping those smaller portions. But line I said.. time will tell. And I wish you luck on this one here!
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Geinstein
Shroomery addict



Registered: 01/25/18
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Re: Teach a newb to grow magic [Re: Hippiebait]
#25922493 - 04/08/19 07:08 AM (5 years, 9 months ago) |
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Quote:
Hippiebait said:
So here’s the coir I been experimenting with. Is it ok? Mycelium does eat it up. Also I’m in northern Maine it is very very very dry is that matters any.
Mmmmmm use bricks
Ye claimed matters Don't worry you'll figure it out.
--------------------
Nothing breads nothing
Edited by Geinstein (04/08/19 01:15 PM)
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