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OfflineSubfinder
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Registered: 07/01/18
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25917930 - 04/05/19 04:29 PM (5 years, 9 months ago)

I thought so too. Looks like satellite colonies.

Still potentially salvageable. You'll need to work on your sterile technique. Never allow your hands or tools to go over your work.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Subfinder]
    #25917962 - 04/05/19 04:54 PM (5 years, 9 months ago)


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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25917970 - 04/05/19 04:57 PM (5 years, 9 months ago)

So this is the recommended tub design? Little 3/4 inch holes? They are placed right correct?

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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918047 - 04/05/19 05:34 PM (5 years, 9 months ago)

tub looks about right but it seems like your holes are super high. How high is that line?

And your plates.... I hate to be the one to inform you, but.... Things aren't looking too good my friend. I see plenty of bacteria in a lot of your dishes but most dominantly in the first. You see all that weirdness around the pin. Was that there before you put the pin in there? That's either a serious bacterial bloom or the sneakiest lighting trick ever. you may be able to snatch some myc up from the 6 oclock position after and if it outgrows the bacteria. Don't jump the gun too much. Wait for the myc to clear the bacterial goo.

Even then you may pick some up that is riding along with the myc. a  few more transfers and you  may be able to clean it up.

the second pick looks like it maybe doing well but i can't tell due to the blur. Third pick looks alright but definitely has a little bacterial colony at the 7 oclock position. This means there is probably more lurking around.

third and fourth LC pics look totally fucked. If that is a test from an LC i'd recommend trashing the LC.

I can't tell much of anything from the last two pics.

I understand that the pins are  from an open grow and that they are not going to be clean, but the amount of bacteria on the first plate is highly concerning and almost certainly did not come from that pin (in my opinion)

subfinder, the advice to "let it grow out 3/4 of the plate" is not generally considered to be "correct". When working to get away from contams, i like to snatch it up as soon as it sufficiently clears the contam. This is never the same, plate to plate. Sometimes i'm snatching up clean myc while the contaminated side of the transfer hasn't even leapt off the wedge yet.

Edited by verum subsequentis (04/05/19 05:38 PM)

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918079 - 04/05/19 05:51 PM (5 years, 9 months ago)

First pic is a close up of the lc. The second two are of the gt pin clones that may be able to be saved. The holes on the tub are 4” high is that to high?

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918128 - 04/05/19 06:28 PM (5 years, 9 months ago)

The holes will be fine at 4 inches. I don't know why they looked so high to me. Your agar has bacteria all over the place. maybe you aren't pressure cooking it well enough. How'd you go about cooking the agar?

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918131 - 04/05/19 06:31 PM (5 years, 9 months ago)

I mixed it well put it in a mason jar and I cooked it on high in my presto for 15 minutes and then gave it time to cool before pouring in a Sab

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Offlineverum subsequentis
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918414 - 04/05/19 08:44 PM (5 years, 9 months ago)

Do you have any signs of growth on leftover uninnoculated plates?

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: verum subsequentis]
    #25918846 - 04/06/19 04:48 AM (5 years, 9 months ago)

They are in a ziplock in the fridge should I take them out and watch? There’s deffinatly some plates that are horrible with bacteria but some look clean to me🤷‍♂️ Do I just not know what I’m looking at or could it be that you are all seeing some dust and some left over Lysol on the outside of the dishes? I’m gonna take up close pics and seperate what I “think is clean and what I think is not”

Edited by Hippiebait (04/06/19 06:37 AM)

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918915 - 04/06/19 06:19 AM (5 years, 9 months ago)

Some of the mycelium in a few of my plates has outrun the bacteria unless I’m missing something. I’ll post pics in a little while and see if I should transfer yet

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25918975 - 04/06/19 07:23 AM (5 years, 9 months ago)

So to me “after cleaning the outside of the petris of Lysol.” It looks like the first 4 are relatively clean besides maybe a little spec or two? The last 3 are trash?

Should I begin transfers from the first two pins and the grain? if so Where from? The grain is the truely amazing equador cluster I would love to get going!! The two pins are from a big cluster of golden teacher that wasn’t aloud to mature🤷‍♂️

Edited by Hippiebait (04/06/19 07:29 AM)

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Invisiblenube424
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Registered: 12/03/17
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919022 - 04/06/19 07:57 AM (5 years, 9 months ago)

U got bacteria all over the place. Ur best bet would be to let those first 3 pin clones grow out for another day then transfer.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: nube424]
    #25919040 - 04/06/19 08:12 AM (5 years, 9 months ago)

Thanks! That’s what I was thinking but unsure. The last 3 plates I may as well just throw away correct?

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Invisiblenube424
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919043 - 04/06/19 08:15 AM (5 years, 9 months ago)

Definitely. It's not even worth it. But atleast there isn't TOO MUCH bacteria on the first 3. Just avoid it when u take transfers. Before u transfer, always hold the plate up to the light so u can see if there's bacteria hidden under the mycelium.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: nube424]
    #25919052 - 04/06/19 08:22 AM (5 years, 9 months ago)

That makes sense for sure! I have the genetics I would like to use on the first plate and the second best on the second two plates so that works out.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919067 - 04/06/19 08:29 AM (5 years, 9 months ago)

What would u folks do wit 40 quarts of grain u didn’t clean up on agar but recover with the shake test? Should I throw them all in a tub and cover with damp verm and hope for the best?

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Invisiblenube424
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919072 - 04/06/19 08:32 AM (5 years, 9 months ago)

40 quarts? U can make like 8-10 monos... pics? If they're clean fruit them. If they don't look good, throw them out :shrug:

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: nube424]
    #25919101 - 04/06/19 08:58 AM (5 years, 9 months ago)

They fully recover in 48 hours after a shake but I don’t want to even waste the materials if it’s most likly just an experiment as I have plenty of cakes going I’ll just wait for a clean culture but if I could get a pound or so off those grains it’s worth growing them out.

Edited by Hippiebait (04/06/19 09:01 AM)

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Invisiblenube424
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Re: Teach a newb to grow magic [Re: Hippiebait]
    #25919126 - 04/06/19 09:15 AM (5 years, 9 months ago)

That's a pint jar... not a quart. But Yea, I would grow them out as long as they're not contaminated.

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OfflineHippiebait
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Re: Teach a newb to grow magic [Re: nube424]
    #25919132 - 04/06/19 09:18 AM (5 years, 9 months ago)

I’ll give it a try. I was gonna just throw them away cause it seams like I get horrible results as far as ms grows go. Maybe I’ll just go with a super high spawn ratio🤷‍♂️

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   MagicBag.co All-In-One Bags That Don't Suck


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