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Offlinecrabbs
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Registered: 07/19/18
Posts: 89
Last seen: 4 years, 9 months
sad day
    #25910894 - 04/02/19 03:42 AM (5 years, 9 months ago)

This was my third run of mini tubs. The first 2 came out ok.

I just lost all three tubs to green mold about 8-10 days into colonization.

ratio was 1:1.5 spawn to verm/core.

What I did different.

* no lining in the tubs
*didn't colonize in the dark
*only had one layer of micro pore tape over the holes.
*I pre soaked the spawn before mixing into tubs with sub.

The temp was similar to the other tubs if anything a bit cooler.

I noticed from day one a massive amount of water coating the roof heavy drops and running down the walls. Could this be because I pre soaked the spawn? mmm

... I'm a fool, i didn't notice that the season change caused the midday sun to shine directly onto my tubs for hours and over 2-3 days before I noticed. Moved them, seven days later green mold everywhere.

Was is the direct sun in the early days of colonization?

Thanks for listening.

Edited by crabbs (04/19/19 09:47 PM)

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OfflineSubfinder
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Registered: 07/01/18
Posts: 1,025
Loc: Pennsylvania
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Re: sad day [Re: crabbs]
    #25910935 - 04/02/19 04:25 AM (5 years, 9 months ago)

How did you get things started? MS to grain? Agar?

Before I started using agar I had similar problems pretty regularly. Get your spawn cleaned up and your problems will likely go away.

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InvisibleFeasoghorm


Registered: 10/24/18
Posts: 4,384
Re: sad day [Re: Subfinder]
    #25910938 - 04/02/19 04:40 AM (5 years, 9 months ago)

:whathesaid: Apparently contams can go unnoticed and then bloom during fruiting.

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OfflineSubfinder
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Re: sad day [Re: Feasoghorm]
    #25910941 - 04/02/19 04:45 AM (5 years, 9 months ago)

Quote:

Timboslice said:
:whathesaid: Apparently contams can go unnoticed and then bloom during fruiting.



That's for sure. A little fresh air always gets it to show it's ugly green face!

Agar is not a step you want to skip...unless you don't mind cultivating mold. I prefer shrooms.

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Offlinecrabbs
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Registered: 07/19/18
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Re: sad day [Re: Subfinder]
    #25910959 - 04/02/19 05:18 AM (5 years, 9 months ago)

wild print to agar, transfer about 5 times to get clean (RR vids) then onto grain. All was going well I thought grain to grain+master each time to keep the strain going.
I still have the grain going now, I really hope it's not in the current grain masters.
I'm kinda freak'n a bit.

I gave to much away me thinks, getting nervous :crazy:


--------------------
*DISCLAIMER*
Everything I say on here is BULLSHIT!! I LIE.

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InvisibleMr Piggy
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Re: sad day [Re: crabbs] * 1
    #25911086 - 04/02/19 07:49 AM (5 years, 9 months ago)

Contaminates come from dirty spawn, sorry buddy.

5 transfers from wild with not much experience under your belt means it's probably still contaminated.

Keep doing transfers and work on your sterile technique, you'll get it.


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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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InvisibleSunnyDayze
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Re: sad day [Re: Mr Piggy]
    #25911197 - 04/02/19 08:52 AM (5 years, 9 months ago)

It also sounds to me like the water was over field capacity.  The red flag for me is you pre-soaking the spawn.  I am not an experienced grower like many on here so I could be wrong.  I do also agree with Mr.Piggy about your spawn probably having contams.

Sorry for your loss!

:feelssadman:


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OfflineJoeyddr
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Registered: 11/08/18
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Re: sad day [Re: SunnyDayze]
    #25911210 - 04/02/19 08:58 AM (5 years, 9 months ago)

It will get better the more you practice man, when i first started doing this I got so bummed about a tub turning green, then i learned proper agar tek, how to use a sab, and G2G, and finally i have jars on rotation.  Now if i see contam i just shrug dump it into the earth and start a new tub/shoebox.


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InvisiblemushboyMDiscord
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Re: sad day [Re: crabbs]
    #25911246 - 04/02/19 09:13 AM (5 years, 9 months ago)

Quote:

crabbs said:
* no lining in the tubs
*didn't colonize in the dark
*only had one layer of micro pore tape over the holes.
*I pre soaked the spawn before mixing into tubs with sub.





i dont line.
no one colonizes in the dark.
so do most of us
dunking spawn is dumb imo.

the sun didnt ruin the grow. your spawn waas probably bad:shrug:

got pics:begger:

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OfflineGeinstein
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Re: sad day [Re: mushboy]
    #25911366 - 04/02/19 10:56 AM (5 years, 9 months ago)

Don't soak spawn if done it a few times its a bad idea
Most probably your spawn like MushBoy said
Or
It could be the pooling water

Again as MushBoy said photos


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Nothing breads nothing

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Offlinecrabbs
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Registered: 07/19/18
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Re: sad day [Re: Geinstein]
    #25919573 - 04/06/19 02:52 PM (5 years, 9 months ago)

Here's a pic.

Cheers


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*DISCLAIMER*
Everything I say on here is BULLSHIT!! I LIE.

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OfflineLogicaL ChaosM
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Re: sad day [Re: crabbs]
    #25919574 - 04/06/19 02:53 PM (5 years, 9 months ago)

Is it still a sad day?

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Offlinecrabbs
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Re: sad day [Re: LogicaL Chaos] * 1
    #25919944 - 04/06/19 06:32 PM (5 years, 9 months ago)

well yep, still sad days haha There is  green shit all over it the tub in the pic.
I tossed em days ago.

I have 12 jars of the same spawn at full colonization. I don't want to put it all to tubs if it's contaminated grain.

Can I keep 9 of the 12 jars in the fridge and just do one tub with the remaining 3 grain jars?

Thanks


--------------------
*DISCLAIMER*
Everything I say on here is BULLSHIT!! I LIE.

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Offlinecrabbs
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Registered: 07/19/18
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Re: sad day [Re: crabbs]
    #25940958 - 04/18/19 04:14 AM (5 years, 8 months ago)

Anyway, things have gotta be on the up from here...
Trich and a sour smell has been everywhere, so jars have been cleaned and grains tossed.

Since before things went to green shit, another thing I noticed I had changed was, where I buy my rye grain, they both look the same. And I always sterilize at 15psi for 90min.

*could changing rye grain be a problem? sterilizing should sterilize right into the grain right?


--------------------
*DISCLAIMER*
Everything I say on here is BULLSHIT!! I LIE.

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OfflineSubfinder
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Registered: 07/01/18
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Re: sad day [Re: crabbs]
    #25940970 - 04/18/19 04:48 AM (5 years, 8 months ago)

Bump it up to 2 hours. I found that when I did that my contam rate went way down.

Could be I got better at SAB and agar work too. Probably both.

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Offlinecrabbs
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Registered: 07/19/18
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Re: sad day [Re: Subfinder]
    #25944656 - 04/19/19 09:51 PM (5 years, 8 months ago)

Does this look right to you?


They look thick and full. The master has been in the fridge for a few months... You help would be greatly appreciated.
Cheers


--------------------
*DISCLAIMER*
Everything I say on here is BULLSHIT!! I LIE.

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InvisibleFeasoghorm


Registered: 10/24/18
Posts: 4,384
Re: sad day [Re: crabbs]
    #25944670 - 04/19/19 10:00 PM (5 years, 8 months ago)

Looks like 3 transfers of healthy white myc.

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Offlinesouthbounpachyderm
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Registered: 07/20/18
Posts: 282
Last seen: 4 years, 5 months
Re: sad day [Re: crabbs]
    #25944683 - 04/19/19 10:04 PM (5 years, 8 months ago)

The picture is a little too blurry to see detail but yeah it doesn't look too bad.


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