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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Monocultures & cloning & PE oh my!
#25904009 - 03/29/19 01:33 PM (5 years, 9 months ago) |
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Im still super green. Feel the strong need to know what im looking at here. Wud appreciate advice.
 Done some 40 plates in my little sab. Plates not shown have no myc yet.

 Had some interesting contams.
Prolly gonna transfer from this bugger right here:

At the moment im curious as to why these two plates have this kinda ringed growth.

Edited by Feasoghorm (04/03/19 01:55 PM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Feasoghorm]
#25904024 - 03/29/19 01:44 PM (5 years, 9 months ago) |
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Quote:
Timboslice said: At the moment im curious as to why these two plates have this kinda ringed growth.
 
I've heard people say both that ringed growth is based on a light cycle (the light affects how it grows when it is on/off), also that it means possibly hidden contams.
I buy into the light cycle theory! Would love to hear other's thoughts.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: Feasoghorm]
#25904034 - 03/29/19 01:50 PM (5 years, 9 months ago) |
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You have everything from nice plates to moldy plates ... some of those you can save and others might as well toss, the very 2 on bottom might as well dump, the one you say you want to transfer has alot of strains going on and I would transfer from all around that plate... the four plates at top I would only try to save the bottom one, most of the ones in the group pic looks nice and you're just going to have to decipher the good fromc bad ...
You're far very far from an isolate but can get there with time and patience
Shaper there is myc that will push rings like that and sometimes its juts the myc but that to me looks moldy and not healthy myc
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25904046 - 03/29/19 02:00 PM (5 years, 9 months ago) |
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Quote:
Boogieman47 said: Shaper there is myc that will push rings like that and sometimes its juts the myc but that to me looks moldy and not healthy myc
Yeah, I refrained from commenting on that part because I wasn't sure. It seemed likely that it wasn't good simply from the size of the myc compared to others.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: ShaperDreaming]
#25904051 - 03/29/19 02:03 PM (5 years, 9 months ago) |
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If that was a clone I def would clean it up but if its ms and have other plates of the same def no need to even play with it ...
Shit like that is hard to tell with myc cause sometimes it is so finicky I probably have tossed healthy plates away due to them being off in my eyes .. it's just something we gotta decipher you know ?
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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25904084 - 03/29/19 02:18 PM (5 years, 9 months ago) |
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Gotcha. Much abliged to the both of you.
Ya them four contam plates i keep above my fridge jst to see what will happen. Satisfies my inquisitive nature.
The proposed transfer plate is really where my attention is at. I made all these to learn, try isolating, and kinda jst to weed out obvious contams and tiger drop the rest in rye, and haphazardly do a tub or two with wat turns out to have only mild bac contam, jst to get some fruits going. But i may be at the point where that jst wont due for me anymore.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25904086 - 03/29/19 02:19 PM (5 years, 9 months ago) |
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Quote:
Boogieman47 said: Shit like that is hard to tell with myc cause sometimes it is so finicky I probably have tossed healthy plates away due to them being off in my eyes .. it's just something we gotta decipher you know ?
Lol I'm still so new I've played out most of my plates. When I hit 100 plates I bought a box of petris, so now I have 500. I'll likely start tossing shit that looks funny.
Here's one that took me back 3 transfers after I decided... yup! I'd isolated not-mushrooms:
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: ShaperDreaming]
#25904117 - 03/29/19 02:35 PM (5 years, 9 months ago) |
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Apes hard to clean up from spores... you could do hot pours on that or a sandwich
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 11 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25904125 - 03/29/19 02:40 PM (5 years, 9 months ago) |
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Quote:
Boogieman47 said: Apes hard to clean up from spores... you could do hot pours on that or a sandwich
Yeah, lol, I went back and cleaned it up well enough to get NAPES, which fucking blew my damn mind. That's my fav part of all this work, taking new routes and finding new places. Agar lead me to something I want to try to clone and isolate for good phenos. A year ago I thought mushrooms might grow from spores like plants do from seeds Weird journeys ya'll
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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Re: Agar porn, isolates, and rather serious undertones [Re: ShaperDreaming]
#25907836 - 03/31/19 01:17 PM (5 years, 9 months ago) |
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 Transfers are fun.
 Cud someone advise me as to wat im looking at here?..With the myc almost avoiding certain spots. Those spots have that sorta halo thing going on though, like 2mrrw morn them spots be filled in. What is that halo shiny sheen that stretches out to meet the outer myc anyway? Jst light hyphal growth that appears as a shiny halo cuz my eyes aint 25x magnification?
Ya know wat, fuck it...Imma get me some magnification today.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: Feasoghorm]
#25907849 - 03/31/19 01:22 PM (5 years, 9 months ago) |
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I dont know what causes that I avoid those spots on transfers but I have alot of plates like that as well even the most cleanest looking plates... every plate has a small transparent ring of myc it is where the myc is colonizing... but when there are big spots of it it sketches me out ... so get the nice rhizo that's further away
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25908930 - 04/01/19 03:51 AM (5 years, 9 months ago) |
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agreed. Or... in the name of science, also take a transfer specifically from that spot (i suspect bacteria) and see how she grows out on the new plate. I think i've done that a few hundred times but can't remember. I definitely avoid any and all fucky situations on agar as a rule of thumb. And, as stated earlier, i have learned to throw fucking ugly ms plates in the trash and not waste anymore time on them
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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Gotcha.
Some transfers from the two "best" plates:
  

These appear generally healthy & clean so i'll prolly run some grain with them:

These were all nasty looking so imma tossin' em.

And these never did anything. Have no idea why. Exposed one to free air this morn, fucking coughed and kinda spat on it. Keeping it with the other contam plates. See if it even grows anything:
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: Feasoghorm]
#25911973 - 04/02/19 04:50 PM (5 years, 9 months ago) |
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Sometimes you can take too small of a cut and if the blade is still hot it will stunt the growth ... happens to me alot I'll try to cut too small and sizzle the myc too much Haha. .. those transfers look nice .. there are some nice plates in that batch pretty much all of them are transferable if you wanted to take them to more agar but some of those I wouldnt get near grain with
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25911980 - 04/02/19 04:55 PM (5 years, 9 months ago) |
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if you coughed on and spat on a plat, it'll absolutely grow some shit. I tested that out. Still haven't gotten around to the semen test. I wonder if i can clone myself.
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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Lol!..the semen test. Ahh that's good shit. I did, i hacked all in it like someones ill mannered shitty kid.
Them blanks jst had spores dropped on them. Hey, which ones wouldnt you go near grain with Boogie?
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 2 years, 2 months
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25912006 - 04/02/19 05:09 PM (5 years, 9 months ago) |
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Quote:
Boogieman47 said: Sometimes you can take too small of a cut and if the blade is still hot it will stunt the growth ... happens to me alot I'll try to cut too small and sizzle the myc too much Haha. .. those transfers look nice .. there are some nice plates in that batch pretty much all of them are transferable if you wanted to take them to more agar but some of those I wouldnt get near grain with
That's why you dip the blade into uncolonised agar first.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Agar porn, isolates, and rather serious undertones [Re: poisoned]
#25912008 - 04/02/19 05:09 PM (5 years, 9 months ago) |
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truth
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Agar porn, isolates, and rather serious undertones [Re: Feasoghorm]
#25912014 - 04/02/19 05:12 PM (5 years, 9 months ago) |
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the ones with the red dots I would def transfer again the just the yellow I dont think are too bad but I'd still transfer atleast another round ... the others I would drop
Yeah I get too excited sometimes and just dive in lmao
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Feasoghorm


Registered: 10/24/18
Posts: 4,384
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Re: Agar porn, isolates, and rather serious undertones [Re: Boogieman47]
#25912076 - 04/02/19 05:46 PM (5 years, 9 months ago) |
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Koolsville. Got enough left to drop in grain to get some boomers going. Still waiting on that god damn PE to pump out anything more that a few side dongers.
Trash:

Imma mess with them plates that did nothing. Mine are self explanitory but my roomy isnt very "scientific"; i made him contaminate one and he put a piece of steak and a piece of garlic in it. More afraid of that one than i am the ass plate.

Edited by Feasoghorm (04/02/19 07:20 PM)
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